Preliminary physico – phytochemical and phytocognostical evaluation of the leaves parts and evaluation of herbal ointment using leaves of Achyranthes aspera L. Leaf extract

Abstract

Herbal plants are huge sources of neutraceuticals, prevent the different disease or maintain healthy life. Plant produces primary metabolites for their basic survival and secondary metabolites for their ecological, taxonomical and biochemical differentiation and diversity. Achyranthes aspera L. (Family Amaranthaceae) are broadly used in traditional system of medicine throughout different part of India, South Andaman Island, Pacific Island, Baluchistan, Tropical Asia, America, and Australia. It has great medicinal importance like to stomach tonic, diuretic, laxative, anthelmintic, anti hyperlipidemic, expectorant, anti-inflammatory, anti-bacterial, anti-fungal, hypoglycemic, anti-asthmatic and anti-allergic etc. The study of diseases and their treatment are important part of our ancient time worldwide. The knowledge of medicinal plants must have been accumulated in the course of many centuries. Herbal medicine prepare different part of plant are used. Herbal drug is design as the alternative formulation for the external use in the form of ointment. For the clinical use the herbal ointment apply externally on human body. The main aims of this research are Preliminary physico–phytochemical & phytocognostical evaluation of the leaves parts and preparation of herbal ointment from the Achyranthes aspera. Achyranthes aspera L. plants used as antibacterial activity. Under this research work, it is design as herbal ointment with the help of Ethanolic extract of Achyranthes aspera and determines the anti-bacterial capacity on it. In vitro study the antibacterial activity of the Ethanolic extracts of dried leaves of Achyranthes aspera L. are determine by using the Agar cup plate method versus different bacteria such as Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa etc. By blending the Ethanolic extract of Achyranthes aspera L. (10 % w/w) into aqueous cream we formulated herbal ointment. The emulsifying agent and simple ointment bases are added in above herbal ointment for increase the antibacterial effectiveness during in vitro evaluation. The current study deals with the characterization of morphological features, determination of physical constant such as the total cash value, water soluble ash value were 11.25%, 5.25% respectively. Loss of weight drying was 12.9%, foaming index>100, swelling index were1.5cm, the percent yield for petroleum ether 5.25%, chloroform 6.34%, ethanol 7.65%, and aqueous 8.35%, and determination of formulation and evaluation of herbal ointment using Achyranthes aspera L. leave extract.