Identification and characterization of phenolic compounds in hydro methanolic extract of Achyranthes aspera (HMEA) by UPLC and MALDI-TOF-MS and in vivo antioxidant activity

Abstract

Achyranthes aspera is an omnipresent herb of medicinal herbal group, used traditionally as medicine to cure different types of diseases. The components of hydro-methanolic extract of Achyranthes aspera (HMEA), are screened and tentatively identified using UPLC-PDA and MALDI-TOF-MS techniques. The phenolic compounds tentatively identified are quinic acid (m/z 192.040), chlorogenic acid (m/z 354.0894), kaempferol (m/z 286.0917), quercetin (m/z 302.0895) and chrysin (m/z 254.0654) present in the various ratios in which concentration level of chlorogenic acid and kaempferol are determined considering their peak intensity. To the best of our knowledge this is the first report on the characterization of HMEA using UPLC and MALDI-TOF-MS techniques to demonstrate the presence of various phenolic compounds and their antioxidant enzymes activity in vivo. The enzymes which showed increased activity are GST, GR, CAT, and SOD while activity of LDH decreased. These phenolic compounds have potential to be used as anti-cancerous molecules.