Excessive Fluoride Research Articles

‘Off–On’ determination of lead (Pb2+) and fluoride (F−) ion in fish and wastewater samples using N, S co-doped carbon quantum dots (N, S-CQDs)

Lead is a global priority pollutant. Its presence in aquatic systems is harmful to the human health. Fluoride is essential to the human body, especially in dental health. However, excess fluoride in the body can lead to serious health concerns. Therefore, a simple approach to monitoring lead and fluoride in environmental samples is paramount. In this study, we synthesized N and S co-doped carbon quantum dots under the hydrothermal method by employing citric acid, glutamine, and sodium sulphide (Na2S) as precursors. Characterization of the developed nanosensor was carried out using Fourier transform infrared spectroscopy (FTIR), high-resolution transmission electron microscopy (HRTEM), photoluminescence (PL) spectrophotometer, ultraviolet–visible spectroscopy (UV–Vis), and X-ray diffraction (XRD). The as-prepared nanosensor is spherical with an average particle diameter of 3.45 ± 0.86 nm and emits light in the green region of the spectrum. This material was employed as an ‘on–off’ and ‘off–on’ fluorescent sensor to determine Pb2+ and F− rapidly and selectively. The fluorescence was quenched (turned off) in the presence of Pb2+ because of the strong interaction between Pb2+ ions and the surface functional groups of the as-synthesized material. Subsequently, the quenched fluorescence of the N, S-CQDs + Pb2+ system was restored (turned on) upon the introduction of F− ions, owing to the formation of ionic bonds between Pb2+ and F−. The N S-CQDs were selective towards Pb2+. At the same time, the N, S-CQDs + Pb2+ system exhibited selectivity towards F− ions amidst other ions with low detection limits (LODs) of 13.35 nM and 43.17 nM for Pb2+ and F−, respectively. The dynamic quenching mechanism was suggested based on the absorption spectra and lifetime results. Satisfied recoveries of 89.30–116.40% for Pb2+ and 90.22–115.05% for F- (RSD < 5) were obtained in practical samples of wastewater and fish. We believe that the as-synthesized N S-CQDs can effectively serve as reliable, accurate, and swift nanosensor for detecting Pb2+ and F− in environmental samples.

Alleviation of fluoride-induced colitis by tea polysaccharides: Insights into the role of Limosilactobacillus vaginalis and butyric acid

Endemic fluorosis has gained increasing attention as a public health concern, and the escalating risk of colitis resulting from excessive fluoride intake calls for effective mitigation strategies. This study aimed to investigate the potential mechanisms underlying the alleviation of fluoride-induced colitis by Tea polysaccharides (TPS). Under conditions of excessive fluoride intake, significant changes were observed in the gut microbiota of rats, leading to aggravated colitis. However, the intervention of TPS exerted a notable alleviating effect on colitis symptoms. Antibiotic intervention and fecal microbiota transplantation (FMT) experiments provided evidence that TPS-mediated relief of fluoride-induced colitis is mediated through its effects on the gut microbiota. Furthermore, TPS supplementation was found to modulate the structure of gut microbiota, enhance the relative abundance of Limosilactobacillus vaginalis in the gut microbiota, and promote the expression of short-chain fatty acid (SCFAs) receptors in colonic tissue. Notably, L. vaginalis played a significant role in alleviating fluoride-induced colitis and facilitating the absorption of butyric acid in the rat colon. Subsequent butyric acid intervention experiments confirmed its remarkable alleviating effect on fluoride-induced colitis. Overall, these findings provide a potential preventive strategy for fluoride-induced colitis by TPS intervention, which is mediated by L. vaginalis and butyric acid.

Fluorinated benzothiadiazole fluorescent probe based on ICT mechanism for highly selectivity and sensitive detection of fluoride ion

Excessive fluoride ion (F-) in the environment can affect health and even endanger life when ingested by the human body. However, most fluoride probes have the disadvantages of low sensitivity and long detection time. Herein, fluorescent probe 3a is successfully synthesized by linking two acetylenyltrimethylsilyl groups at both ends of the fluorinated benzothiadiazole core. After the addition of F- to 3a, the emission at 436 nm is significantly quenched and slightly blue-shifted. It is confirmed by electrospray ionization high-resolution mass spectrometry (ESI-HRMS) and density functional theory calculations (DFT) that these changes are due to the F- triggered Si-C bond cleavage and the subsequent inactivation of intramolecular charge transfer (ICT). The detection limit and response time of probe 3a for F- are 10-8 mol/L and 25 s, respectively. Importantly, fluorescent material 3a can be processed into portable test tools for the visual detection of fluoride ion.

Efficient removal of fluoride from water: Adsorption performance of MgO-x adsorbents derived from Mg-MOF-74 and insights from DFT calculations

Magnesium oxide (MgO), an inexpensive and non-toxic substance, is considered an excellent and environmentally friendly fluoride adsorbent. In this study, a series of MgO-x materials (MgO-300, MgO-500, MgO-700 and MgO-800) were obtained by calcination with Mg-MOF-74 as a precursor, and they were used for efficient fluoride removal from wastewater. Both MgO-500 and MgO-700 exhibit excellent fluoride adsorption capacity in the pH range of 3.00–10.00. Specifically, their maximum adsorption capacities are 151.11 mg g−1 and 173.30 mg g−1, respectively. By analyzing the adsorption isotherm and kinetic data, it is confirmed that the adsorption of F− by MgO-x is more in line with the Langmuir isotherm model and the pseudo-second-order kinetic model. Interestingly, the adsorption rate of MgO-500 was significantly faster than that of MgO-700. MgO-x exhibited excellent repeat application performance, with MgO-500 maintaining 75.5 % fluoride ion removal efficiency and 81.5 % regeneration efficiency after five adsorption/desorption cycles. FTIR and XPS analyses show that the adsorption mechanism of fluorine on MgO-x is mainly the result of ligand exchange and electrostatic attraction. Density functional theory calculations show that the adsorption of fluorine on the MgO (110) plane is more favorable than on the (100) plane. This study demonstrates that magnesium oxide derived from Mg-MOF-74, as an economical and environmentally friendly material, has great potential to remove excess fluoride from water.

Design and development of rare earth elements anchored pectin/chitosan integrated magnesia hybrid composite for effective defluoridation of water

Fluoride pollution in drinking water is a worldwide concern arises from natural and human actions. The excessive fluoride present in drinking water leads to fluorosis. In this study, dual polysaccharide biopolymers namely pectin and chitosan were incorporated with magnesia followed by imprinted with lanthanum and cerium ions gives PCML (pectin/chitosan/magnesia/lanthanum) and PCMC (pectin/chitosan/magnesia/cerium) hybrid materials for fluoride adsorption investigation. The enhanced defluoridation capacities (DCs) of PCML and PCMC composites were seen as 4.976 and 4.972 mg g−1 by 30 min individually. To decide the impact of various affecting parameters viz., dosage, initial fluoride concentration, pH, contact time and challenging anions were examined in batch method. The pH results show the utmost DCs of PCML and PCMC composites was noted at pH 6 and in co-ions experiments, the significant competition found with bicarbonate ion owing to the superior electronic building of bicarbonate ion. The defluoridation equilibrium data of PCML and PCMC composites follows Langmuir, pseudo-second-order and intraparticle diffusion models. The positive ΔH° value as 3.04 kJ mol−1 for PCML composite and 2.88 kJ mol−1 for PCMC composite were exposed that the endothermic nature of fluoride adsorption occurs onto PCML and PCMC composites. The defluoridation mechanism of PCML and PCMC composites were attained by complexation, ion-exchange and electrostatic interaction. The reusability results shows that the synthesized PCML and PCMC composites can be reused upto six cycles. Field trail result demonstrates the synthesized PCML and PCMC composites were applicable at field conditions by reducing fluoride concentration below 1.5 mg/L.

High-performance tin perovskite transistors through formate pseudohalide engineering

The lack of high-performance p-type semiconducting materials hinders the integration of complementary metal-oxide semiconductors with well-established n-type metal-oxide counterparts. Although tin halide perovskites are promising p-type material candidates, their practical implementation is hindered by excessive hole concentrations and difficulties in precisely controlling crystallization, which leads to poor device performance and yield. In this paper, we propose a formate pseudohalide engineering method to overcome these issues and demonstrate high-performance tin perovskite thin-film transistors (TFTs). The incorporation of formate anion greatly suppresses the vacancy defects at the surfaces of the perovskite films with an increase in crystallinity and grain size. This reduces the hole concentration and eliminates the dependence on the addition of excessive tin fluoride for hole suppression. Hence, high-performance TFTs with a high average field-effect hole mobility of 57.34 cm2 V−1 s−1 and on/off current ratios surpassing 108 can be achieved, approaching p-channel low-temperature polysilicon devices.

Groundwater chemical evolution characteristics and human health risk assessment in Shicheng County, Jiangxi Province.

Groundwater plays a pivotal role in the water resources of Shicheng County; however, the issue of excessive fluoride content in groundwater and its associated health risks often goes unnoticed. Groundwater assumes a crucial role in the hydrological dynamics of Shicheng County; nevertheless, the matter concerning elevated levels of fluoride within groundwater and its accompanying health hazards frequently evades attention. The hydrogeochemical analysis, obscure comprehensive water quality assessment based on cloud model, and probabilistic human health risk assessment using Monte Carlo simulation were conducted on 34 collected water samples. The findings indicate that the predominant groundwater hydrochemical types are SO4·Cl-Na and HCO3-Na. The processes of rock weathering and cation exchange play crucial roles in influencing water chemistry. Groundwater samples generally exhibit elevated concentrations of F-, surpassing the drinking water standard, primarily attributed to mineral dissolution. The concentrations of F- in more than 52.94% and 23.53% of the groundwater samples exceeded the acceptable non-carcinogenic risk limits for children and adults, respectively. Considering the inherent uncertainty in model parameters, it is anticipated that both children and adults will have a probability exceeding 49.36% and 30.50%, respectively, of being exposed to elevated levels of F ions in groundwater. The utilization of stochastic simulations, in contrast to deterministic methods, enables a more precise depiction of health risks. The outcomes derived from this investigation possess the potential to assist policymakers in formulating strategies aimed at ensuring the provision of secure domestic water supplies.

Regulating effect of miR-132–3p on the changes of MAPK pathway in rat brains and SH-SY5Y cells exposed to excessive fluoride by targeting expression of MAPK1

BackgroundAlthough the changes of mitogen-activated protein kinase (MAPK) pathway in the central nervous system (CNS) induced by excessive fluoride has been confirmed by our previous findings, the underlying mechanism(s) of the action remains unclear. Here, we investigate the possibility that microRNAs (miRNAs) are involved in the aspect. MethodsAs a model of chronic fluorosis, SD rats received different concentrations of fluoride in their drinking water for 3 or 6 months and SH-SY5Y cells were exposed to fluoride. Literature reviews and bioinformatics analyses were used to predict and real-time PCR to measure the expression of 12 miRNAs; an algorithm-based approach was applied to identify multiply potential target-genes and pathways; the dual-luciferase reporter system to detect the association of miR-132–3p with MAPK1; and fluorescence in situ hybridization to detect miR-132–3p localization. The miR-132–3p inhibitor or mimics or MAPK1 silencing RNA were transfected into cultured cells. Expression of protein components of the MAPK pathway was assessed by immunofluorescence or Western blotting. ResultsIn the rat hippocampus exposed with high fluoride, ten miRNAs were down-regulated and two up-regulated. Among these, miR-132–3p expression was down-regulated to the greatest extent and MAPK1 level (selected from the 220 genes predicted) was corelated with the alteration of miR-132–3p. Furthermore, miR-132–3p level was declined, whereas the protein levels MAPK pathway components were increased in the rat brains and SH-SY5Y cells exposed to high fluoride. MiR-132–3p up-regulated MAPK1 by binding directly to its 3′-untranslated region. Obviously, miR-132–3p mimics or MAPK1 silencing RNA attenuated the elevated expressions of the proteins components of the MAPK pathway induced by fluorosis in SH-SY5Y cells, whereas an inhibitor of miR-132–3p just played the opposite effect. ConclusionMiR-132–3p appears to modulate the changes of MAPK signaling pathway in the CNS associated with chronic fluorosis.

A Comparative Evaluation of Arginine Complex Combined With Flouride and Two Standard Non-Fluoridated Remineralizing Agents: An In Vitro Study.

Background Dental caries represents a dynamic process, often reversible in its early stages. Fluoride has conventionally served as the cornerstone for remineralization and early caries arrest. However, excessive fluoride intake can lead to both local and systemic toxicity. Hence, there's a pressing need to develop adjunct therapies that enhance fluoride's efficacy while minimizing its dosage. This study aims to assess and compare the remineralization potential of a novel combination comprising arginine bicarbonate and fluoride against established technologies such as Bioactive glass (NovaMin Technology; Sensodyne Repair and Protect, GlaxoSmithKline, UK) and CPP-ACP technology (GC Tooth Mousse; Tokyo Japan). Materials and methods The experiment utilized extracted premolars designated for orthodontic extraction. The initial evaluation employed the DIAGNOdentTMfluorescence method. Subsequently, teeth underwent demineralization and were measured for values. Following this, the teeth were subjected to seven cycles of remineralization, after which moment values were reassessed. Statistical analysis was performed on the recorded values. Results Participants were divided into six groups (BR-A, AR-A, BR-B, AR-B, BR-C, AR-C). T-tests demonstrated significant reductions in moment values within each group, indicating the effectiveness of all remineralizing agents. Group C exhibited the most substantial difference (-6.900 ± 0.4), followed by Group A and Group B. ANOVA analysis revealed statistically significant differences among all three groups (p=0.016). Tables showed significant distinctions between the remineralizing values of Groups A and C and Groups B and C (p=0.02 and 0.002, respectively), with no discernible distinction between Groups A and B. Conclusion The study elucidates the superior efficacy of the arginine complex with fluoride combination compared to CPP-ACP and Bioactive Glass individually. This finding underscores the potential of the novel combination therapy in enhancing remineralization while minimizing fluoride dosage, thus presenting a promising strategy for addressing early-stage dental caries.

Mediation of mitochondrial DNA copy number and oxidative stress in fluoride-related bone mineral density alteration in Chinese farmers.

Excessive fluoride can adversely affect bone mineral density (BMD). Oxidative stress and mitochondrial dysfunction are crucial mechanisms of health damage induced by fluoride. Here, a cross-sectional survey involving 907 Chinese farmers (aged 18-60) was carried out in Tongxu County in 2017, aiming to investigate the significance of mitochondrial DNA copy number (mtDNAcn) and oxidative stress in fluoride-related BMD change. Concentrations of urinary fluoride (UF), serum oxidative stress biomarkers, including total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA), as well as relative mtDNAcn in peripheral blood were determined. The multivariable linear model and mediation analysis were performed to assess associations between UF, oxidative stress, and relative mtDNAcn with BMD. Results showed that GSH-Px levels increased by 6.98 U/mL [95% confidence interval (CI) 3.41-10.56)] with each 1.0mg/L increment of UF. After stratification, the T-AOC, relative mtDNAcn, and BMD decreased by 0.04mmol/L (-0.08 ~ -0.01), 0.29-unit (-0.55 ~ -0.04), and 0.18-unit (-0.33 ~ -0.03) with every 1.0mg/L elevation of UF in the excessive fluoride group (EFG, adults with UF > 1.6mg/L), respectively. Furthermore, T-AOC and relative mtDNAcn were favorably related to the BMD in the EFG (β = 0.82, 95%CI 0.16-1.48 for T-AOC; β = 0.11, 95%CI 0.02-0.19 for relative mtDNAcn). Mediation analysis showed that relative mtDNAcn and T-AOC mediated 15.4% and 17.1% of the connection between excessive fluoride and reduced BMD, respectively. Findings suggested that excessive fluoride was related to lower BMD in adults, and the decrement of T-AOC and relative mtDNAcn partially mediate this relationship.

Carbon doping enhances the fluoride removal performance of aluminum-based adsorbents.

Excessive fluoride presence in water poses significant environmental and public health risks, necessitating the development of effective remediation techniques. Conventional aluminum-based adsorbents face inherent limitations such as limited pH range and low adsorption capacity. To overcome these challenges, we present a facile solvent-thermal method for synthesizing a carbon-doped aluminum-based adsorbent (CDAA). Extensive characterization of CDAA reveals remarkable features including substantial carbon-containing groups, unsaturated aluminum sites, and a high pH at point of zero charge (pHpzc). CDAA demonstrates superior efficiency and selectivity in removing fluoride contaminants, surpassing other adsorbents. It exhibits exceptional adaptability across a broad pH spectrum from 3 to 12, with a maximum adsorption capacity of 637.4mg/g, more than 110 times higher than alumina. The applicability of the Langmuir isotherm and pseudo-second-order models effectively supports these findings. Notably, CDAA exhibits rapid kinetics, achieving near-equilibrium within just 5min. Comprehensive analyses utilizing Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) offer detailed insights into the mechanisms involving electrostatic attraction, ion exchange, and ligand exchange. Carbon-based groups play a role in ligand exchange processes, synergistically interacting with the unsaturated aluminum structure to provide a multitude of adsorption sites. The exceptional attributes of CDAA establish its immense potential as a transformative solution for the pressing challenge of fluoride removal from water sources.

Optimal fluoride levels in pediatric toothpastes: A review of the literature

Aim:Through this literature review, we seek to determine the optimal level of fluoride present in pediatric toothpastes, identifying the recommended amount according to scientific literature, in addition to evaluate the effects of fluoride on children's dental health and its effectiveness in prevention of caries and also identify the risks of excessive fluoride exposure. Materials and methods: Searches were carried out for articles published in the last 5 years in electronic databases such as PubMed, Scopus, Scielo, ScienceDirect, UpToDate, Google Academic. The search used a combination of the following keywords: "toothpaste" AND "pediatrics" AND "dosage" AND "fluoride". Results: 14,118 abstracts were registered: 1,407 from PubMed, 1,043 from Scopus, 355 from ScienceDirect, 10,200 from Google Academic, 913 from Scielo, 200 from UpToDate. After reviewing the abstracts, 14,095 records were removed according to the inclusion and exclusion criteria, leaving 31 full-text articles for examination. Finally, 19 full-text articles were included and evaluated in this review. Conclusion: The presence of adequate concentrations of fluoride in toothpastes has a positive influence in oral health, especially on the prevention of dental caries. Several reviewed studies reveal that the optimal amount of fluoride in pediatric toothpastes should be greater than 1000 ppm but not exceed 1500 ppm to obtain the maximum anti-caries effect and prevent adverse effects such as dental fluorosis.

New insights into defluoridation via induced crystallization: Implications of phosphate species regulation for process efficiency and economics

Excess fluoride (F) at low concentrations in groundwater remains a challenge. F removal via fluidized-bed induced crystallization has been receiving increasing attention; however, the precipitant cannot be fully utilized. This results in generation of by-products, inadvertently increases the treatment cost, and impedes the assessment of product purity. In this study, phosphate species regulation was employed to improve the F removal efficiency without decreasing the phosphate utilization efficiency. It shows that F concentration could be reduced from 4.75 to 0.45 mg·L−1, with P concentration in the effluent being only 0.05 mg·L−1 at a P:F molar ratio of 3, involving appropriately mixed phosphate species. Hydroxyapatite (HAP) crystallization, subsequent F adsorption, and directly induced fluorapatite (FAP) crystallization occurred simultaneously in the fluidized bed, which was indirectly confirmed through intermittent operation of the process. The products of the induced crystallization process were FAP and a small amount of HAP, as determined qualitatively using X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR) tests. Overall, by regulating the phosphate species, the F removal efficiency is maintained, the cost of the precipitant is lowered, and the residual P concentration in the effluent is reduced. The process results in products with FAP as the main component, which can be used as raw material for the phosphoric acid purification process.

A V-shaped bis-coumarin based fluorescence probe for F- detection in tea infusions and potable water and bioimaging applications in living systems

Fluorine (F) is a pivotal element in the formation of human dental and skeletal tissues, and the consumption of water and tea constitutes a significant source of fluoride intake. However, prolonged ingestion of water and tea with excessive fluoride content can lead to fluorosis, which poses a serious health hazard. In this manuscript, a novel turn-on fluorescent probe DCF synthesized by bis-coumarin and tert-butyldiphenylsilane (TBDPS) was introduced for detecting F- in potable water and tea infusions. By leveraging the unique chemical affinity between fluoride and silicon, F- triggers the silicon-oxygen bond cleavage in DCF, culminating in a conspicuous emission of yellow fluorescence. Validated through a succession of optical tests, this probe exhibits remarkable advantages in terms of superior selectivity, a low detection limit, a large Stokes shift, and robust interference resistance when detecting inorganic fluoride. Moreover, it can serve as portable test strips for on-site real-time identification and quantitative analysis of F-. Furthermore, the application of DCF for in-situ monitoring and imaging of F- in zebrafish and soybean root tissues proved its significant value for F- detection in both animal and plant systems. This probe potentially functions as an efficient instrument for delving into the toxic mechanisms of fluoride in physiological processes.

Analysis of Toxic Effects of Fluoride on Ovine Follicular Granulosa Cells Using RNA-Seq.

Fluoride is abundant in the environment and is an essential trace element in living organisms. However, prolonged excessive fluoride intake can lead to fluorosis, which poses a threat to the reproductive health of animals and humans. Although previous research has mainly focused on animal models, the impact of fluoride on ovine follicular granulosa cells (GCs) has not been comprehensively elucidated. This study employed RNA-Seq technology to elucidate the toxic effects of fluoride on ovine follicular GCs and its mechanism of action. Culturing primary ovine follicular GCs in vitro and subjecting them to fluoride treatment revealed 3218 differentially expressed genes (DEGs), with 2278 upregulated and 940 downregulated. Significantly, this study unveiled fluoride's induction of endoplasmic reticulum (ER) stress in cells, triggering a cascade involving the PERK pathway factor ATF4, leading to cell death via DDIT3/CHOP activation and the subsequent upregulation of CHAC1, ATF3, ERO1α, and TRIB3. These findings provide crucial insights into the toxicity of fluoride in ovine, offering a foundation for mitigating fluoride-related losses in the farming industry.

Water quality and sustainable solutions for drinking water supply in the Taquari-Antas watershed, RS, Brazil

Access to safe drinking water is a fundamental human right and a critical component of sustainable development. This study is conducted in the context of the Taquari-Antas Hydrographic Basin, Rio Grande do Sul, Brazil, and assesses the quality of drinking water in 101 rural properties, focusing on identifying problems of potability, especially regarding excessive fluoride concentration, which is a known problem for the water in this region. The study involves the collection of water samples from rural properties, a survey to understand water use and quality perception by users, and water quality analysis using established analytical methodologies. Results show that 58.4% of the investigated rural properties have potability issues, with excessive fluoride being the most prevalent concern, affecting 39.6% of properties. High fluoride levels have significant health implications, including dental and skeletal fluorosis, with global relevance. To address this issue, activated bone charcoal filtration systems were installed in ten properties, effectively reducing fluoride concentrations to within acceptable limits for human consumption. These defluoridation systems are characterized as sustainable, cost-effective, and scalable, offering a practical solution for regions facing similar challenges. Keywords: groundwater, rural properties, water stewardship.

Bifidobacterium Relieved Fluoride-Induced Hepatic and Ileal Toxicity via Inflammatory Response and Bile Acid Transporters in Mice.

Fluoride is a pervasive environmental contaminant. Prolonged excessive fluoride intake can inflict severe damage on the liver and intestines. Previous 16S rDNA sequencing revealed a decrease in ileal Bifidobacterium abundance during fluoride-induced hepatointestinal injury. Hence, this work aimed to investigate the possible mitigating function of Bifidobacterium on hepatointestinal injury caused by fluoride. Thirty-six 6-week-old C57BL/6J mice (equally divided between males and females) were allotted randomly to three groups: Ctrl group (distilled water), NaF group, and NaF + Ba group (100 mg/L NaF distilled water). After 10 weeks, the mice were given 1 × 109 CFU/mL Bifidobacterium solution (0.2 mL/day) intragastrically in the NaF + Ba group for 8 weeks, and the mice in other groups were given the same amount of distilled water. Dental damage, bone fluoride content, blood routine, liver and intestinal microstructure and function, inflammatory factors, and regulatory cholic acid transporters were examined. Our results showed that fluoride increased glutamic-oxalacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT) activities, and the levels of lipopolysaccharide (LPS), IL-1β, IL-6, TNF-α, and IL-10 levels in serum, liver, and ileum. However, Bifidobacterium intervention alleviated fluoride-induced changes in the above indicators. In addition, Bifidobacterium reduced the mRNA expression levels of bile acid transporters ASBT, IBABP, OST-α, and OST-β in the ileum. In summary, Bifidobacterium supplementation relieved fluoride-induced hepatic and ileal toxicity via an inflammatory response and bile acid transporters in the liver and ileum of mice.

Bonding to Fluorosed Teeth: A Review of the Literature.

Excessive fluoride availability during tooth formation can cause structural alterations in enamel and dentin. These alterations may negatively impact the adhesion of various dental materials to teeth with dental fluorosis. The aim of this review of literature is to identify updates in bonding to fluorosed teeth and summarize relevant recommendations. Findings from the available literature suggest that bonding procedures may be carried out reliably on most fluorosed teeth with consideration to the severity of fluorosis and the employment of additional bond-enhancing measures for the severely involved teeth.

The association between Dental Fluorosis and COL1A2 gene polymorphism among a Tunisian Population

Dental fluorosis (DF) is a prevalent developmental defect of tooth enamel caused by exposure to excessive fluoride, with the severity dependent on various factors. This study aimed to investigate the association between DF and a specific genetic polymorphism (rs412777) in the COL1A2 gene among a Tunisian population. A case-control study was conducted from July to November 2022, involving a total of 95 participants including 51 cases and 44 controls. Dental examinations and genetic analysis were performed to assess the relationship between the COL1A2 gene polymorphism and DF.The results of allelic distribution revealed that A allele carriers were significantly protected against (DF) when compared to those with the C allele (C vs. A, p = 0.001; OR = 0.375 (0.207–0.672)). This suggests a strong correlation between the presence of the C allele and the risk of developing DF. Additionally, significant association between the CC genotype of rs412777 and an increased risk of DF was found under both codominant and dominant genetic models (P = 0.002 and P < 0.001 respectively).The findings suggest that genetic predisposition plays a relevant role in the development of DF. Further research is needed to explore the potential use of genetic markers for DF and their implications for public health. This study provides the first insights into the genetic factors associated with DF in the Tunisian population, contributing to our understanding of this prevalent dental condition.

MiR-214-3p Regulates Apoptosis of Ameloblasts under Excessive Fluoride via PI3K/AKT Signaling Pathway

MiR-214-3p Regulates Apoptosis of Ameloblasts under Excessive Fluoride via PI3K/AKT Signaling Pathway