Excess Sludge Production Research Articles

Effective sludge activation in a sequencing batch reactor (SBR) towards aerobic granular sludge (AGS) technology

Activated sludge is a biological treatment that commonly deals with the problem of excess sludge production. As advanced technology, aerobic granular sludge is expected to address the drawbacks of the activated sludge system, including the excess sludge issue. This study aims to investigate the characteristics and performance of the activation process of excess sludge. The study was conducted in a sequencing batch reactor with different substrate compositions in three phases. The activation process with biodegradable substrate supported by nutrients and micronutrients consisting of cations successfully enhanced the growth of microorganisms to 6720 mg/L of MLSS and 5450 mg/L of MLVSS. The settling performance of the sludge increased, marked by a low average of SVI5 of 63.89 mL/gTSS and SVI30 of 45.16 mL/gTSS. The treatment improved COD removal to 90 % and ammonia, nitrate, and phosphate removal to 98 %, 47 %, and 37 %, respectively. The granulation process with micronutrients as cation addition in 25 mg/L has not been achieved through the experiment in 18 days indicated by the ratio of SVI30 to SVI5 on the average of 0.71. Therefore, the findings suggest that the study of substrate and cation concentration to accelerate the granulation is proposed towards AGS technology and its application to achieve a sustainable environment.

Overlooked pyrite-mediated heterogeneous Fenton processes: Mechanisms of surface hydroxyl radical generation and associated decontamination performance

Pyrite-based Fenton-like processes have been extensively studied for wastewater decontamination; however, most relevant studies placed excessive emphasis on the homogeneous Fenton reaction mediated by aqueous Fe2+, resulting in the proposed technologies facing issues such as additional acid requirements for pH adjustment and excessive iron sludge production. Herein, through in situ shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS), custom dual-chamber reactor experiments, and a series of control experiments, significant hydroxyl radical generation was identified during the pyrite/H2O2 process, while the dominant reactive iron species was verified to the structural Fe sites on the pyrite surface, rather than structural Fe(II) in secondary iron minerals and surface adsorbed Fe2+. Consequently, even with significant suppression of the homogeneous Fenton pathway, the pyrite/H2O2 process exhibited significant degradation efficiency for sulfamethoxazole (SMX) at pH 4. Moreover, the pyrite/H2O2 process was found to selectively remove 50 μM of pollutants with high affinity for pyrite (bisphenol A, carbamazepine, nitrobenzene, and SMX), even in the presence of 50–100 mM methanol. Compared to the typical iron-based reductive catalyst (zero-valent iron, ZVI), pyrite mediated a Fenton process with greater potential for practical applications at pH 4, achieving a 43.75-fold reduction in iron sludge production and almost doubling the H2O2 utilization efficiency. Additionally, in contrast to ZVI, minimal iron oxide formed on the pyrite surface during the oxidation process. Thus, after seven cycles of degradation experiments, the decontamination efficiency of the pyrite/H2O2 process remained stable. These findings are crucial for understanding the complex environmental behavior of pyrite in both natural and engineering processes and provide a new perspective for the efficient utilization of pyrite resources as well.

Influence of the oxidative phosphorylation uncoupler m-chlorophenol on activated sludge production, nutrients removal efficiency and bacterial community structure of a membrane reactor system

In this work, the effects of the metabolic uncoupler meta-chlorophenol (m-CP) on excess sludge production and performance of an MBR system, as well as on activated sludge communities, were investigated. MLVSS were significantly reduced from 8410 mg/L to 5175 mg/L during the first week of the addition of 125 mg/L m-CP, while, thereafter, biosolids were restored to the level prior to uncoupler’s addition. Adding m-CP resulted in more severe effects on nitrifiers rather than organoheterotrophs. Both alkalinity, dissolved oxygen (DO), electrical conductivity (EC) and pH data evidenced the adverse effect of m-CP on the nitrification process. PO43--P concentration drastically raised immediately after m-CP addition from 2.48 ± 0.35 mg/L to 11.84 mg/L, possibly due to the uncoupling of P-release and the hydrolysis of phosphorus reserves. Diversity was highly affected by the addition of m-CP, resulting in the significant decrease of Chao1, Shannon and Fisher indices. By establishing stable operating conditions after m-CP addition, Pseudomonas, Arcanobacterium, Trichococcus, Kocuria and Actinobaculum raised from almost undetectable levels (0.12 % of the total relative abundance) to 35.9 %, 17.8 %, 15.1 %, 11.5 % and 5.7 %, respectively, indicating a massive shift in bacterial community structure, while no nitrifying taxa were detected after m-CP addition.

Should wastewater treatment plants' operational mode radically change to minimize GHG emissions?

The operation of municipal wastewater treatment plants (WWTPs) invariably results in significant emission of greenhouse gases (i.e., CH4, N2O, and CO2) into the atmosphere. We propose to consider a radical change in the way municipal WWTPs are operated, with the aim of minimizing GHG emissions while recycling most of the nutrient mass. The means to this end are to reduce the WWTP energy demand while maximizing the recovery of resources (phosphorus, ammonia, methane). The suggested concept involves operating the activated sludge process at a low sludge retention time (SRT < 2 d), i.e., under conditions that maximize the heterotrophic mass yield and eliminate nitrification. The ammonia concentration that remains in the water (considering N in the excess sludge and struvite production in the sludge-dewatering supernatant line) would be separated from the WWTP effluents using a unique ion-exchange material (ZnHCF), which would be regenerated using a low-volume 4 M NaCl solution. The ammonia would be then stripped at high pH and re-adsorbed by an acidic solution for reuse as fertilizer. The high bacterial yield and lack of nitrification in the aerobic step are expected to boost methane yield 3–4-fold, induce lower oxygen consumption, and most importantly, yield much lower N2O release. An approximate energy mass balance shows the concept to merit further consideration, owing to the potential significant reduction in N2O(g) emissions and recovery of resources. Empirical work followed by LCA is required to corroborate the hypothesis presented herein.

The anaerobic exposure time (AET) as a novel process parameter in the anaerobic side-stream reactor (ASSR)-based process for excess sludge minimization

Minimization of excess sludge produced by wastewater treatment plants has become a topical theme nowadays. One of the most used approaches to achieve this aim is the anaerobic side-stream reactor (ASSR) process. This is considered affected by the hydraulic retention time (HRT) of the anaerobic reactor, the anaerobic sludge loading rate (ASLR) and the sludge interchange ratio (SIR), although, studies available in the literature did not reflect a clear relationship with the sludge minimization yields.To overcome this, a novel parameter namely anaerobic exposure time (AET) was defined and related to reduction of the observed yield coefficient (Yobs) in a lab-scale plant implementing the ASSR process. Furthermore, the AET was validated by performing a detailed and thorough review of previous literature. Excess sludge production was successfully reduced (10–60 %) with the increase of the AET (7.9–13 h/d), although maintaining the same HRT in the ASSR and a constant sludge interchange ratio (SIR) (100 %). A strong correlation (Pearson = 0.763) was found between the AET, and the Yobs reduction reported in previous studies, also indicating a linear relationship (R2 = 0.92) between these parameters. Contrarily, the correlation between the Yobs with the ASLR and the ASSR-HRT resulted moderate (Pearson = 0.186) or weak (Pearson=−0.346), respectively. Overall, while operating at low AET (< 6 h), maintenance and uncoupling metabolism were found the main sludge reduction mechanisms. Increasing the AET (>8 h) favoured the occurrence of extracellular polymeric substances (EPS) hydrolysis and endogenous decay mechanisms, which improved excess sludge reduction. To conclude, the AET could be considered a reliable parameter to be used for design or control purposes for the ASSR-based process.

Enhancement of medium-chain fatty acids production from sludge anaerobic fermentation liquid under moderate sulfate reduction

In recent years, there has been a marked increase in the production of excess sludge. Chain-elongation (CE) fermentation presents a promising approach for carbon resource recovery from sludge, enabling the transformation of carbon into medium-chain fatty acids (MCFAs). However, the impact of sulfate, commonly presents in sludge, on the CE process remains largely unexplored. In this study, batch tests for CE process of sludge anaerobic fermentation liquid (SAFL) under different SCOD/SO42− ratios were performed. The moderate sulfate reduction under the optimum SCOD/SO42− of 20:1 enhanced the n-caproate production, giving the maximum n-caproate concentration, selectivity and production rate of 5.49 g COD/L, 21.4% and 4.87 g COD/L/d, respectively. The excessive sulfate reduction under SCOD/SO42− ≤ 5 completely inhibited the CE process, resulting in almost no n-caproate generation. The variations in n-caproate production under different conditions of SCOD/SO42− were all well fitted with the modified Gompertz kinetic model. Alcaligenes and Ruminococcaceae_UCG-014 were the dominant genus-level biomarkers under moderate sulfate reduction (SCOD/SO42− = 20), which enhanced the n-caproate production by increasing the generation of acetyl-CoA and the hydrolysis of difficult biodegradable substances in SAFL. The findings presented in this work elucidate a strategy and provide a theoretical framework for the further enhancement of MCFAs production from excess sludge.

First application of the novel anaerobic/aerobic/anoxic (AOA) process for advanced nutrient removal in a wastewater treatment plant

The stringent effluent quality standards in wastewater treatment plants (WWTPs) can effectively mitigate environmental issues such as eutrophication by reducing the discharge of nutrients into water environments. However, the current wastewater treatment process often struggles to achieve advanced nutrient removal while also saving energy and reducing carbon consumption. The first full-scale anaerobic/aerobic/anoxic (AOA) system was established with a wastewater treatment scale of 40,000 m3/d. Over one year of operation, the average TN and TP concentration in the effluent of 7.53 ± 0.81 and 0.37 ± 0.05 mg/L was achieved in low TN/COD (C/N) ratio (average 5) wastewater treatment. The post-anoxic zones fully utilized the internal carbon source stored in pre-anaerobic zones, removing 41.29 % of TN and 36.25 % of TP. Intracellular glycogen (Gly) and proteins in extracellular polymeric substances (EPS) served as potential drivers for post-anoxic denitrification and phosphorus uptake. The sludge fermentation process was enhanced by the long anoxic hydraulic retention time (HRT) of the AOA system. The relative abundance of fermentative bacteria was 31.66 - 55.83 %, and their fermentation metabolites can provide additional substrates and energy for nutrient removal. The development and utilization of internal carbon sources in the AOA system benefited from reducing excess sludge production, energy conservation, and advanced nutrient removal under carbon-limited. The successful full-scale validation of the AOA process provided a potentially transformative technology with wide applicability to WWTPs.

Effects of ozone on activated sludge: performance of anaerobic digestion and structure of the microbial community

Abstract The treatment and disposal of activated sludge are currently challenging tasks in the world. As a common biological engineering technology, biological fermentation exists with disadvantages such as low efficiency and complex process. Ozone pretreatments are commonly applied to improve this problem due to their high efficiency and low cost. In this study, the significant function of ozone in anaerobic fermentation gas production was verified with excess sludge. Compared with other untreated sludge, ozone pretreatment can effectively degrade activated sludge. After ozone treatment and mixing with primary sludge, the methane production of excess sludge increased by 49.30 and 50.78%, and the methanogenic activity increased by 69.99 and 73.83%, respectively. The results indicated that the mixing of primary sludge with excess sludge possessed synergistic effects, which contributed to the anaerobic fermentation of excess sludge. The results of microbial community structure exhibited that methanogenic processes mainly involve hydrogenogens, acidogens and methanogens. The relative abundance of both bacteria and microorganisms changed significantly in the early stage of hydraulic retention time, which coincided exactly with the gas production stage. This study provided a feasible pretreatment strategy to improve sludge biodegradability and revealed the role of microorganisms during anaerobic digestion.

Sewage sludge minimisation by OSA-MBR: A pilot plant experiment

This study presents the excess sludge minimisation in a Membrane Bioreactor (MBR) system by an Oxic Settling Anaerobic (OSA) process. The pilot plant was fed with real wastewater and OSA was operated with two different hydraulic retention times (HRT), respectively 4 (Period II) and 6 h (Period III) and compared to an MBR (Period I). Multiple parameters/variables were monitored: sludge minimisation, nitrogen and carbon removal, membrane fouling, and biokinetic behaviour through respirometry. With respect to the current literature, greenhouse gas emissions were also here monitored, often neglected. Results demonstrated that combining MBR and OSA systems can significantly reduce excess sludge production (89.7%, in Period III and 59.7% in Period II, compared to Period I). However, Period III presented better PO4–P removal efficiencies but worse performances in the other parameters (COD, NH4 and Total Nitrogen). No substantial variation in membrane fouling was obtained over the experimental periods. Finally, the HRT increase in the anaerobic reactor promoted a N2O–N increase inside the unaerated reactors, highlighting the need for a trade-off between sludge minimisation and GHG emission.

Optimization of Fe(II)/SPC Sludge Conditioning and Dewatering Process Based on Response Surface Methodology: Full-Scale Application

Sludge conditioning is a crucial step in sludge dewatering aimed at minimizing excessive sludge production. The Fenton process, which harnesses oxidative radicals to dismantle extracellular polymeric substances (EPS) and microorganisms, has been unequivocally proven to enhance sludge dewaterability. However, the widespread adoption of the Fenton process is hampered by its high costs and logistical challenges in transportation. In contrast, the Fe(II)-activated sodium percarbonate (Fe(II)/SPC) process has emerged as a promising technology for sludge conditioning due to its remarkable performance and safe operation. However, limited information is available regarding the optimization of Fe(II)/SPC for sludge conditioning and dewatering at full scale. This study conducted the sludge conditioning and dewatering process within a full-scale wastewater treatment plant, utilizing the response surface methodology (RSM) to optimize the Fe(II)/SPC process. Furthermore, this study investigated its impact on sludge structure and compared the economic benefits of the Fe(II)/SPC process with other full-scale conditioning processes. The results of bound water and LDH analysis revealed that the Fe(II)/SPC process not only degraded EPS but also disrupted microbial cells, thereby releasing intracellular water. Based on the RSM results, we successfully established a polynomial prediction model to determine the optimal capillary suction time (CST) and moisture content. The optimal parameters determined through RSM were an initial pH of 3.02, Fe(II) dosage of 0.05 g/g TSS, and SPC dosage of 0.07 g/g TSS. The validation test confirmed the accuracy of the prediction results, with the conditioned sludge exhibiting a CST of 31.6 s and a moisture content of 51.47%. Furthermore, when compared to the PFS and Fenton processes, the Fe(II)/SPC process demonstrated higher economic efficiency and safety, while maintaining good dewatering performance. Overall, the Fe(II)/SPC treatment shows promise as a prospective sludge dewatering and conditioning process.

Ascorbic acid-enhanced Fe(III)/peracetic acid process for the degradation of diclofenac: Treatment efficiency, mechanism and influencing factors

To overcome the sluggish reduction from Fe(III) to Fe(II) and excessive iron sludge production in Fe(III)/peracetic acid (PAA) process, ascorbic acid (AA), an environmental-friendly and natural reductant, was proposed in this study to boost the oxidation ability of Fe(III)/PAA process by facilitating the cycling of Fe(III)/Fe(II). AA-enhanced Fe(III)/PAA process exhibited remarkable acceleration in the degradation of diclofenac (DCF), with the removal rate of DCF being around 51 times higher than that without AA. Multiple reactive species of FeIVO2+, R-O, and OH were involved in the abatement of DCF in the AA/Fe(III)/PAA process. FeIVO2+ was confirmed as the predominant contributor for the elimination of DCF, whereas R-O and OH also participated in DCF abatement. In the AA-enhanced Fe(III)/PAA process, AA rapidly complexed with Fe(III) to produce the Fe(III)-AA complex, which was subsequently decayed to generate Fe(II), and then reactive species were generated by activating PAA with Fe(II) to degrade DCF through the pathways of dichlorination-cyclization, decarboxylation, and hydroxylation. The acute toxicity of the reaction solution employing BioFixlumi as a toxicity indicator decreased with the abatement of DCF. Common inorganic ions, including Cl−, SO42−, CO32−, and NO3−, had no adverse influence on DCF degradation, while humic acid and common cations (Zn2+, Ca2+, and Mg2+) inhibited DCF abatement. AA/Fe(III)/PAA process was highly effective for DCF removal in actual water samples, including surface water and underground water. AA/Fe(III)/PAA process was prone to degrading micropollutants with higher fractions of deprotonated species and lower ionization potentials, and performed with high selectivity towards micropollutants. Overall, the outstanding oxidation capacity and environmental friendliness of AA/Fe(III)/PAA process render it a potential technology to remove micropollutants in wastewater treatment.

Reduction of sewage sludge and N2O emissions by an Oxic Settling Anaerobic (OSA) process: The case study of Corleone (Italy) wastewater treatment plant

Biosolid management is becoming one of the most crucial issues for wastewater treatment plant (WWTP) operators. The application of the Oxic Settling Anaerobic (OSA) process allows the minimisation of excess sludge production. This study compares conventional activated sludge (CAS) and OSA layouts in a full-scale WWTP (namely, Corleone - Italy). Extensive monitoring campaigns were conducted to assess treatment performances regarding carbon and nutrient removal, greenhouse gas (GHG) emissions, excess sludge production, and biomass activity (by means of respirometric analysis). Results showed that the effluent quality consistently met the Italian discharge limits. However, with the implementation of the OSA process, there was a decrease in ammonium removal efficiency, which could be attributed to reduced nitrifier activity related to reduced biomass production and extended anaerobic conditions affecting the nitrification process. On the other hand, the OSA configuration significantly increased phosphorus removal, indicating a high phosphorus content in the resulting waste sludge. A worsening of the sludge settling properties was observed with the OSA configuration likely due to decreased EPS concentrations. The sludge production in the OSA configuration decreased by 17.3 % compared to CAS. Nitrous-oxide measurements did not show a variation between CAS and OSA configurations, confirming that the OSA process can be a suitable solution for reducing WWTP's carbon footprint.

Effects of the Co-treatment to Municipal Wastewater with Microwave-Irradiated Excess Sludge via an A2/O Process and Its’s Impact on the Excess Sludge Production

Effects of the Co-treatment to Municipal Wastewater with Microwave-Irradiated Excess Sludge via an A²/O Process and Its’s Impact on the Excess Sludge Production

Sulfide recovery using fluidized bed homogeneous crystallization technology to produce nickel sulfide from wastewater that contains sulfides

Sulfide-containing wastewater, characterized by its foul odor, corrosiveness, and toxicity, can endanger human health. Fluidized-bed homogeneous crystallization (FBHC) avoids the excessive sludge production commonly associated with conventional chemical precipitation methods. In this study, FBHC is used to treat sulfur-containing synthetic wastewater. Furthermore, nickel-containing wastewater was utilized as a precipitant in the system, hence the advantage of simultaneous sulfur and nickel removal from the wastewater. The operating parameters, including pH, a precipitant dosage of [Ni2+]0/[S2−]0, and cross-sectional surface loading (LS, kg/m2h) are optimized. The optimum operating conditions of pH 9.8 ± 0.3, [Ni2+]0/[S2−]0 = 0.8, and LS = 1.5 kg/m2h results in total sulfur removal (TR) of 95.7% and crystallization ratio (CR) of 94.8%. The effect of organic compounds (acetic acid, oxalic acid, EDTA, and citric acid) and inorganic ions (NO3−, CO32−, PO43−, F−, and Cl−) on the nickel sulfide granulation process was discussed.

Composite hydrogels based on calcium alginate and polyethyleneimine for wastewater treatment

AbstractWater shortage has risen severely in recent years, confronting mankind with a worldwide challenge, especially as the accessible water resources are further limited by diverse contamination. The most widespread industrial process for water treatment is the activated sludge process, in which, however, excessive sludge production has become an enormous environmental problem worldwide. To overcome this problem, hydrogels possess outstanding potential in view of adsorptive removal of contaminants like heavy metal ions, fertilizers, and dyes. In this paper, we report about the synthesis of biobased alginate hydrogel beads along with polyethyleneimine‐modified composite hydrogels for water treatment. The adsorption of methylene blue as a positively charged and of congo red as a negatively charged model dye is quantitatively investigated, both separately and in combination. In addition, the pH‐dependent adsorption of the dyes is determined. The use of alginate‐based hydrogel systems combines several prospects: they are bio‐based, inexpensive, easily available to a sufficient extent, sustainable, and are applicable in a broad range of wastewater treatment by its charged groups.

Up-flow anaerobic sludge blanket treatment of swine wastewater: Effect of heterologous and homologous inocula on anaerobic digestion performance and the microbial community

The effects of heterogenous (anaerobic sludge from treating distillery sewage, ASDS) and homologous (anaerobic sludge from treating swine wastewater, ASSW) inocula on anaerobic digestion and the microbial community in an up-flow anaerobic sludge blanket treating swine wastewater were compared. The highest chemical oxygen demand removal efficiencies with ASDS (84.8%) and ASSW (83.1%) were obtained with an organic loading rate of 15 kg COD/m3/d. For ASSW compared with ASDS, methane production efficiency was 15.3% higher and excess sludge production was 73.0% lower. The abundance of the cellulose hydrolyzing bacterium Clostridium sensu stricto_1 with ASDS (36.1%) was 1.5 times that with ASSW, while that of Methanosarcina with ASSW (22.9%) was > 100 times that with ASDS. ASDS reduced the content of pathogenic bacteria by 88.0%, while ASSW maintained a low level of pathogenic bacteria. ASSW greatly improved the methane production efficiency of wastewater and is more suitable for treating swine wastewater.

Design and operation insights concerning a pilot-scale S0-driven autotrophic denitrification packed-bed process

Elemental sulfur autotrophic denitrification (S0AD) is viewed as a promising alternative to conventional heterotrophic denitrification due to lower running costs, zero carbon dioxide emission, and minimum excess sludge production. However, its scale-up capability and robustness in treating real-life wastewater have not been convincedly demonstrated. In this study, a pilot-scale S0AD packed-bed with over 1000 m3/d of actual wastewater treatment capacity was operated for 197 days. The S0AD packed-bed could effectively remove nitrate to below 12 mg-NO3−-N/L that is 20% stricter than China’s national standard (15 mg-TN/L). The temperature effect coefficient Q10 was calculated as 1.01, illustrating that denitrification efficiency could be doubled when the temperature increased every 10 °C. Mass balance calculations indicated that 85% of removed nitrate was contributed by S0AD process, and the rest 15% was by heterotrophic and assimilative processes. A total of 2684 kg sulfur was consumed during the course of the experiment which was attributed to 16.1% DO oxidation and 83.9% denitrification. Nitrogen gas produced through denitrification, could be trapped in the S0 packed-bed that was the primary causality of clogging. Daily gas venting instead of conventional back washing, could effectively recover the packed-bed flux and promote denitrification efficiency. In addition, a weekly thorough back washing was deemed necessary to deeply clean the trapped SS and sloughed overgrowing biofilm. The predominant S0AD bacteria belonged to the genus Thiobacillus, was enriched throughout the packed-bed providing robust and stable denitrification. Overall, we provided some guidance to the design and operation of S0AD packed bed in practical engineering.

Low-carbon and high-ammonia nitrogen dispersed wastewater treatment: From “normal-sludge” to “low-sludge” to “no-sludge” modes

Several biological enhancements were implemented in the aerobic tank to address the challenges of treating expressway service sewage (ESS) with low-carbon and high-ammonia nitrogen using A/O-MBR technology, aiming to improve TN removal efficiency and reduce excessive sludge production. A novel moving bed biofilm reactor (MBBR) inoculated with heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria was developed for ESS, and the results showed that HN-AD bacteria significantly improved TN removal efficiency, with an increase of 65% compared to the traditional activated sludge system. High-throughput sequencing revealed that Bacteroidotas contributed significantly to MBBR denitrification, and the genes nirK and nosZ played a significant role in denitrification. The HN-AD biofilm-forming MBBR achieved the transition of ESS treatment from “normal-sludge” mode to the more environmentally-friendly “low-sludge” and “no-sludge” modes by reducing the sludge concentration.

Influence of the Oxic-Settling-Anaerobic (OSA) Process on Methane Production by Anaerobic Digestion of Sewage Sludge

The present study evaluated different sludge-reduction mechanisms in the oxic-settling-anaerobic (OSA) process in terms of their effects on methane productivity by anaerobic digestion of sewage sludge. Two different layouts were investigated for the sludge return from an anaerobic side-stream reactor (ASSR) to the anoxic (scheme A) or the aerobic (scheme B) reactor of a pre-denitrification plant. Biochemical methane-potential (BMP) assays performed on the excess sludge revealed that scheme A promoted an overall increase of methane production in the OSA (20 mLCH4 gVSS−1d−1, +19%), although compared with a control CAS plant a significant decrease in the excess sludge production (31%) was obtained. Operating conditions in scheme A caused the occurrence of cell lysis and EPS hydrolysis, thereby increasing the biodegradability of sludge. In contrast, scheme B favoured the occurrence of uncoupling and a maintenance metabolism that did not involve sludge hydrolysis. Consequently, despite a higher reduction of excess sludge (82%), a significant decrease in methane productivity in the OSA (4 mLCH4 gVSS−1d−1, −41%) was observed. Based on the results, implementing the OSA process may allow high levels of methane production by anaerobic digestion to be maintained if specific sludge-reduction mechanisms are triggered in the waterline, also raising the possibility of co-digestion with other feedstocks.

Upgrading the MBBR Process to Reduce Excess Sludge Production in Activated Sludge System Treating Sewage

Excess sludge production is one of the limitations of the biological activated sludge process. Therefore, the study’s objective is to upgrade the MBBR process to an integrated fixed film-activated sludge (IFAS) process to reduce excess sludge production. Two scenarios were followed in this study to eliminate sludge production in the biological activated sludge process: first, modifying the moving bed biofilm reactor (MBBR) system by increasing the solid retention time (SRT) from 5 to 15 days; and second, upgrading the MBBR process to the integrated fixed-film activated sludge (IFAS) process by applying return activated sludge (RAS) of 50, 100 and 150% with operating hydraulic retention time (HRT) of 6, 12, 14 and 20 h. The results revealed that the first scenario reduced sludge production from 750 to 150 g/day, whereas the second scenario eliminated sludge generation. In the second scenario, operating the system as an IFAS process with complete SRT has eliminated sludge due to sludge decay and cell lysis. In part 3 of the second scenario, the results also showed that the system achieved low effluent pollutants concentrations of 3, 12, 8 and 45 mg/L for BOD, COD, TSS and NO3, respectively. Operating at complete SRT may eliminate sludge production but also result in higher NO3 effluent concentration due to the production of NH3 from sludge decay and cell lysis. To conclude, sludge elimination in an activated sludge system is possible by carefully controlling the process and applying RAS without additional treatment.