Abstract
Several biological enhancements were implemented in the aerobic tank to address the challenges of treating expressway service sewage (ESS) with low-carbon and high-ammonia nitrogen using A/O-MBR technology, aiming to improve TN removal efficiency and reduce excessive sludge production. A novel moving bed biofilm reactor (MBBR) inoculated with heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria was developed for ESS, and the results showed that HN-AD bacteria significantly improved TN removal efficiency, with an increase of 65% compared to the traditional activated sludge system. High-throughput sequencing revealed that Bacteroidotas contributed significantly to MBBR denitrification, and the genes nirK and nosZ played a significant role in denitrification. The HN-AD biofilm-forming MBBR achieved the transition of ESS treatment from “normal-sludge” mode to the more environmentally-friendly “low-sludge” and “no-sludge” modes by reducing the sludge concentration.
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