Evaluation Of Platelet-rich Plasma Research Articles

Evaluation of Platelet-rich Plasma and Microneedling for Facial Skin Rejuvenation

Background: The regenerative properties of platelet growth factors make platelet-rich-plasma (PRP) an attractive modality for treatment of aging skin. The main objective of this study was to determine efficacy and safety of PRP injections and microneedling compared with saline injections in women with aging skin of the face. Methods: In this prospective, randomized clinical trial, 18 women with facial aging were randomized to receive either PRP injections to the unilateral face and saline injections to the contralateral side, or vice versa. Microneedling was performed after injections on the entire face. Physician assessment, photographs, and treatment satisfaction questionnaires were used for outcome assessment at baseline and 16- and 24-week follow-ups. Results: There was no evidence of improvement and suggestion of worsening in skin laxity and rhytides from baseline to weeks 4, 16, and 24 for PRP and saline (all P ≤ 0.004) and no notable difference in skin roughness between baseline and follow-up time points for PRP or saline (all P ≥ 0.19). The degree of change in skin laxity, rhytides, and skin roughness from baseline to follow-up time points was similar for PRP and saline. All patients experienced some degree of pain/discomfort and burning/stinging sensation at treatment weeks 4, 8, and 12 for both saline and PRP. Conclusions: PRP injections did not seem to be effective for treatment of aging skin of the face in women, with no notable macroscopic improvement in appearance when compared with baseline or saline injections. Advanced age of study participants (>45 years) and less-sensitive methods of evaluation may be potential contributing factors to the lack of detected response.

Evaluation of Platelet-Rich Plasma by means of PRGF®-Endoret® protocol in leukemia cats: PDGF-BB and TGF-ß1 valuation.

Feline leukemia virus (FeLV) is a chronic disease that leads to the weakening of a cat's immune system. Platelet-rich plasma (PRP) offers therapeutic effects for multiple diseases, the use of PRP and growth factors (GFs) determination could be an alternative treatment to improve the quality of life in these patients. The objectives of this study were to determine and compare the concentration of platelets (PLTs), red blood cells (RBCs) and white blood cells (WBCs) between samples of whole blood (WB), PRP and platelet-poor plasma (PPP) fractions, and to evaluate the concentration of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor β1 (TGF-β1) in both fractions in FeLV cats using a PRGF®-Endoret® protocol previously standardized in this species. WB was collected from 11 asymptomatic FeLV-positive cats. PRP and PPP was obtained following PRGF®-Endoret® technology according to centrifugation at 265 g for 10 min. Cellular components, RBCs, WBCs, PLTs, and the PDGF-BB and TGF-β1 concentrations in PRP and PPP fractions were determined. PLT in the PRP fraction was statistically higher than WB and PPP fraction, with no statistical differences between WB and PPP. PLT concentration increased 1.4 times in PRP fraction compared to WB. Mean platelet volume (MPV) did not differ significantly between the WB, PRP, and PPP fractions. Compared to WB, the absolute numbers of RBCs and WBCs were decreased by 99% and more than 95% in the PRP and PPP fractions, respectively. TGF-ß1 concentrations increased in PRP vs. PPP, with no changes in PDGF-BB. Based on the degree of PLT enrichment and the absence of RBCs and WBCs, this blood product could be classified as a Pure Platelet-Rich Plasma (P-PRP). The presence of GFs in PRP and PPP samples suggests that the PRGF®-Endoret® methodology is suitable for obtaining PRP in FeLV cats, despite future studies are necessary to optimize the technique, standardize the results and assess clinical efficacy.

Evaluation of platelet-rich plasma (PRP) in the treatment of rotator cuff tendinopathy

Evaluation of platelet-rich plasma (PRP) in the treatment of rotator cuff tendinopathy

Evaluation of Platelet Rich Plasma for Treatment of Female Pattern Hair Loss: A Placebo-Controlled Case Study

Background: Platelet Rich Plasma (PRP) is an autologous concentration of human platelets contained in a small volume of plasma, has been proposed as a potential adjuvant therapy to Female Pattern Hair Loss (FPHL). Despite the widespread use of PRP in numerous fields of medicine, there have been few studies that investigated the clinical efficacy of PRP in FPHL. Methods: Fourteen female patients, age ranged from 20-45 years, diagnosed with female pattern hair loss were enrolled in this single-blinded randomized placebo-controlled study. Fourteen patients were divided randomly into two groups: Group 1 were injected with PRP using insulin syringe and group 2 were injected with normal saline as placebo. All patients were followed at 3 and 6 months after the last treatment sessions. Of the total subjects enrolled, on ten subjects completed the study. In total, 7 (70%) individuals received PRP and 3 (30%) received placebo. During all the treatment sessions, there were no severe adverse events reported. The study yielded investigator, subject and photographic assessments. Results: On the basis of the independent-blinded investigator assessment of change in the patient’s scalp hair growth from baseline, treatment in PRP group demonstrated as scale 1 improvement in 5 subjects (26-50%) and scale 2 improvement in 2 subjects (51-75%) at the final visit as compared to baseline (Fig. 1,2). In contrast, in the placebo group were rated as scale 0 improvement (0-25%). Conclusion: PRP injection for FPHL is a simple, cost-effective and feasible treatment option with good safety profile. However more randomized and multicentric trials with a large number of patients will be required to prove the validity of these results, to obtain these answers and to get more robust statistical results.

Evaluation of platelet-rich plasma vs. platelet lysate: VEGF and PDGF concentration, stability, and shelf life

Cell-free biologicals are a novel method of treating clinical conditions which involve chronic inflammation such as tendonitis and osteoarthritis. Standard approaches for addressing such conditions involve steroids and powerful pain killers, which predispose patients to unfavorable clinical outcomes. Cell-free biologicals like platelet-rich plasma (PRP) and platelet lysate (PL) derived from blood provide a reliable approach to treat chronic inflammation. aPRP is the activated form of PRP and is assumed to contained higher concentration of cytokines. These blood derivatives are safe and can be administered with ease over long periods. Although this approach is widely popular among physicians, it requires scientific standardization of its growth factors and a complete analysis of its shelf life and stability. In this study, we compared platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) in PRP, activated PRP (aPRP), and PL. We analyzed the shelf life at different storage temperature over three weeks. We hypothesized that PL would contain higher concentrations of growth factors than PRP and that different storage temperatures for PL would diminish cytokine expression. Our results demonstrated PL had the highest concentrations of both cytokines, with concentrations slightly diminishing at-80C. aPRP and PRP demonstrated lower concentrations of PDGF and VEGF than PL.

Evaluation of platelet-rich plasma vs steroid in the treatment of sacroiliitis by ultrasound-guided injection in patients with seronegative axial spondyloarthropathies

ObjectiveThe aim was to evaluate the efficacy of ultrasound-guided (USG) sacroiliac injection of platelet-rich plasma (PRP) for sacroiliitis in seronegative axial spondyloarthropathies (SPAs) vs triamcinolone acetonide injection.BackgroundSacroiliitis is considered a hallmark for the diagnosis of seronegative SPA. Sacroiliac joint steroid injection is a commonly used technique. A novel promising injective treatment is PRP.Patients and methodsThis interventional study included 70 patients with seronegative axial SPA, from 2017 to 2019. The patients were divided into two groups: group I received USG injection of triamcinolone acetonide, and group II received USG injection of PRP. Patients were evaluated for pain intensity by visual analog scale (VAS) and Modified Oswestry Disability Questionnaire (MODQ) 4 and 8 weeks after injection and with MRI 8 weeks after injection.ResultsSteroid and PRP were effective in the treatment of sacroiliitis by USG injection. The improvement was significant and lasted longer in group II (68.6% became with mild pain regarding VAS and 74.3% of minimal disability regarding MODQ) as compared with group I after 8 weeks of injection. Patients who received steroids improved dramatically at 4 weeks (60% of patients experience mild pain regarding VAS and 45.7% became with minimal disability regarding MODQ), with decreased efficacy after 8 weeks. With follow-up with MRI, active sacroiliitis can be fully improved by PRP injection (57.1% with normal MRI result after 8 weeks).ConclusionsSteroid and PRP were effective in the treatment of sacroiliitis in seronegative SPA by USG injection, but PRP had a persistent and long-lasting effect.

Evaluation of platelet-rich plasma on hair regrowth and lesional T-cell cytokine expression in alopecia areata: A randomized observer-blinded, placebo-controlled, split-head pilot study

Platelet-rich plasma has shown some promise in the treatment of alopecia areata. To evaluate the effect of platelet-rich plasma on hair regrowth and lesional T-cell cytokine expression in alopecia areata. This was a randomized, placebo-controlled, split-head study involving 27 patients with alopecia areata (Severity of Alopecia Tool score ≥25%). Alopecia patches on either side of the scalp were randomized to receive 3 intradermal injections of platelet-rich plasma or normal saline at monthly intervals and evaluated 3months after the last session. Lesional T-cell cytokine messenger RNA expression was compared pre- and posttreatment in the platelet-rich plasma-treated sites. The mean Severity of Alopecia Tool score did not change significantly compared with baseline with either platelet-rich plasma or placebo injections at any visit; however, the mean percentage reduction in the score in the platelet-rich plasma arm was more than in the placebo arm (9.05%±36.48% vs 4.99%±33.88%; P=.049) at final assessment. The mean interferon gamma (P=.001) and interleukin 17 cytokine (P=.009) messenger RNA expression decreased, whereas the mean interleukin 10 (P=.049) and FOXP3 (P=.011) messenger RNA expression increased significantly after platelet-rich plasma treatment. Small sample size and a relatively short follow-up. Platelet-rich plasma was found to have limited efficacy in alopecia areata. However, it may play a role in restoring immune balance in the alopecic patches.

Evaluation of platelet-rich plasma as a treatment for androgenetic alopecia: A randomized controlled trial

Platelet-rich plasma (PRP) shows promise as an androgenetic alopecia (AGA) treatment. To conduct a randomized placebo-controlled split-scalp study to investigate the effects of PRP on hair regrowth and thickness. Two 7.6-cm×7.6-cm squares were tattooed on the scalps of 35 study participants with AGA. Areas were randomly assigned to intradermal injection with PRP or saline. Participants received 3 monthly treatment sessions with evaluation 3months after the final treatment. Hair density in the PRP-treated area was significantly increased compared with baseline at all visits. At the final assessment, hair density in PRP-treated areas increased from 151±39.82 hairs/cm2 at baseline to 170.96±37.14 hairs/cm2, a mean increase of approximately 20 hairs/cm2 (P<.05). However, hair density in placebo-treated areas also increased from 151.04±41.99 hairs/cm2 to 166.72±37.13 hairs/cm2 (P<.05). There was no significant difference in hair density change between the 2 groups (P>.05). No serious adverse events were reported. Possible PRP diffusion due to split-scalp study design as well as microinjections causing microinjury to both sides. PRP may have benefit in increasing hair density.

Evaluation of platelet-rich plasma from diabetic donors shows increased platelet vascular endothelial growth factor release.

Platelet-rich plasma (PRP) contains pro-angiogenic growth factors including vascular endothelial growth factor (VEGF). Angiogenesis is a necessary component of wound healing in instances of diabetic foot ulcers (DFU). PRP composition varies depending on methods and donor health status. Our group has developed an improved PRP protocol for diabetes treatment. The aims of this study were to examine the levels of the pro-angiogenic factor VEGF in these patient populations with and without diabetes. PRP was prepared using 24 mL of whole blood from 13 diabetic and 10 non-diabetic patients registered at Klinik Hayandra. Whole blood in sodium citrate tubes were centrifuged at 1,000 rpm for 5 minutes followed by plasma separation. Plasma samples were centrifuged at 3,000 rpm for 5 minutes. Upper platelet-poor plasma layers were discarded, leaving 5 mL of concentrated platelet containing plasma (PRP). Concentrated plasma samples were mixed, aliquoted, stored at -86 °C, and pooled for platelet count, VEGF, and total protein analyses. Platelet counting was also performed using fresh whole blood and PRP to measure changes following PRP preparation. Diabetic donors had higher whole blood platelet counts than non-diabetic donors, but this difference was not statistically significant. An average increase of more than 250% in platelet number after PRP preparation using our method was noted in both groups. Freezing-thawing samples at -86 °C lysed more than 90% of PRP platelets regardless of diabetes status. Diabetic PRP had lower mean total protein and higher VEGF concentrations. Lysed platelets from diabetic donors released more VEGF than those from non-diabetic donors. PRP from diabetic donors had higher VEGF content making autologous PRP application a promising treatment for DFU. However, this should be investigated another appropriate clinical trial.

Comparative evaluation of Platelet Rich Plasma (PRP) versus Platelet Rich Fibrin (PRF) scaffolds in regenerative endodontic treatment of immature necrotic permanent maxillary central incisors: A double blinded randomized controlled trial

ObjectivesThe research aims to assess the regenerative potential of Platelet Rich Plasma (PRP) versus Platelet Rich Fibrin (PRF) scaffolds in immature permanent maxillary central incisors with necrotic pulps, clinically and radiographically. Trial designDouble blinded parallel randomized controlled trial was implemented to identify the results. Subject & methodsThe proposed study was conducted among 30 patients with maxillary necrotic permanent immature central incisors but only 26 patients fulfilled the study requirements. Group I was treated with PRP and Group II with PRF scaffolds. Follow up has been done every 3 months for one year. Primary outcomes were measured clinically: Pain, Mobility, Swelling, and Sinus/fistula. Radiographically: increase root length and width. Secondary outcomes were clinically: Discoloration and Sensibility test. Radiographically: increase in bone density measurements and decrease in apical diameter. Standardized radiographs were collected during the follow up period, and radiographic changes were measured by using Image J software. Statistical analysis was performed on 25 patients who had completed the study. ResultsAll 25 patients' teeth were survived during the 12-month follow-up period. PRP showed marginal increase in radiographic root length and width, periapical bone density and a decrease in apical diameter. No statistical significant differences were observed when it was compared with PRF. The teeth which were treated did not respond to sensibility test at the end of the study. PRF displayed statistical significant higher amount of crown discoloration when compared to PRP group. ConclusionsFor necrotic immature teeth, revascularization using PRP is an appropriate alternative to PRF and showed excellent 12-months prognosis.

Comparative Histopathological Evaluation of Platelet-Rich Plasma Injection and Autologous Blood Injection to Temporomandibular Joint: A Pilot Experimental Study

Background: Chronic recurrent TMJ dislocation adversely affects patients’ lives. Various surgical and non-surgical methods have been used for treating recurrent TMJ dislocation. Some non-surgical methods include physiotherapy, occlusal splint, and avoidance of activities causing large-mouth- opening, and autologous blood or Platelet- Rich Plasma- injection to the Temporomandibular Joint (TMJ). Objectives: This experimental study aimed to compare the histopathological evaluation of Platelet-Rich Plasma (PRP) injection and autologous blood (AB) injection to the Temporomandibular Joint. Methods: This experimental study was conducted in an animal lab affiliated to the Gazi University of Medical Sciences, Ankara, Turkey, in 2016. Bilateral TMJs from 12 young New Zealand White rabbits were assessed in the study. The animals were divided into two experimental groups with equal numbers (n = 6) including PRP and AB injection groups. For the experimental groups, 1 mL was injected into the upper joint space and 0.5 mL to the pericapsular tissue. The injections were administered unilaterally. Moreover, 1 mL of isotonic saline was injected into the contralateral sides of TMJ upper joint space and 0.5 mL into the pericapsular tissue to serve as controls for both groups. One month after the experimentation, the animals were sacrificed, and histological analyses were performed for fibrosis and tissue reactions. Results: Collagen fibers and adipose tissue production in the retrodiscal ligament were more in the PRP group than in the AB group. According to the fibrosis classification, similar fibrotic changes were observed in the AB and PRP-injected tissue samples compared to controls. No cartilage degeneration was seen in either of the two groups. Conclusions: Considering the limitations of this study, PRP injection may be an alternative to AB injection for treating chronic mandibular dislocation. Clinicians could safely use the PRP injection technique to increase the success rate of the treatment.

Evaluation of platelet-rich plasma vs intralesional steroid in treatment of alopecia areata.

Alopecia areata (AA) is nonscarring patchy area of hair loss. Platelets rich plasma (PRP) promotes development of new hair follicles. Dermoscopy is a diagnostic tool that helps in evaluation of skin microstructures which are not visible to naked eye. To evaluate safety and efficacy of (PRP) vs intralesional corticosteroid (ILCs) in treatment of AA. This study was conducted on 80 patients of both sexes who had AA. Patients were classified into Group I treated by ILCs and group II treated by (PRP). Results were assessed by dermoscopic evaluation and by hair re-growth score (RGS) at (baseline), 2, 4, 6, 8, and 12weeks. Patients were followed up for 6months. There was greater hair re-growth after treatment in both groups. In group I, (26) patients (65%) showed improvement >70% compared to 29 patients (72.5%) in group II. There was significant re-growth of pigmented hair and decrease in dystrophic hair (P<0.001) by dermoscopic evaluation in both groups. The difference between both groups was insignificant (P=0.57). At follow-up, two (5%) patients in group II had relapse compared to 10 (25%) patients in group I. Platelets rich plasma is safe and promising therapeutic option in AA.

PR575: Evaluation of platelet rich plasma (PRP) with freeze‐dried bone allograft (FDBA) for site preservation of extracted dental sockets for dental implantation

PR575: Evaluation of platelet rich plasma (PRP) with freeze‐dried bone allograft (FDBA) for site preservation of extracted dental sockets for dental implantation

Evaluation of platelet-rich plasma in treatment of alopecia areata

BackgroundAlopecia areata (AA) has major effects on the quality of life and self-esteem, especially in the young patients. Platelet-rich plasma (PRP) promotes hair growth and survival both in vivo and in vitro because of its high concentration of growth factors that stimulate the formation of hair e

Evaluation of Platelet Rich Plasma in the Management of Psoriasis

Psoriasis is a chronic immune-mediated disease affecting approximately 2–3% of general population. It isassociated with significant negative impact on patient’s physical and psychological quality of life along withvulnerability to co-morbidities such as cardiometabolic dysfunction, depression, and so on [1]. to evaluate theefficiency of platelet rich plasma (PRP) in the management of patients with chronic localized recalcitrant plaquepsoriasis.This was a prospective pilot study conducted on 30 cases with chronic localized recalcitrant psoriasisattended Dermatology, Venereology and Andrology outpatient clinics, Benha University Hospitalis. This study wasapproved by Dermatology, Venereology and Andrology Department and Ethics Commitee in the Faculty ofMedicine, Benha University. The study details were discussed with all patients and informed consents wereobtained from them before the begining of the study.PASI score had significantly decreased in group I after 8 and 16weeks of treatment when compared to the baseline (p =0.001). There was also significant decrease in PASI score betweenweek 8, 16 (p =0.0018). PASI score showed no significant difference in group II neither at week 8 nor 16 compared tobaseline (p =0.894).

Comparative evaluation of platelet rich plasma in socket healing and bone regeneration after surgical removal of impacted mandibular third molars.

Background. Surgical removal of mandibular third molars results in pain, swelling and bony defects, causing prolonged postoperative recovery. The growth factors present in platelet-rich plasma (PRP) can accelerate the healing, thereby shortening postoperative recovery period. This study was undertaken to evaluate the role of PRP in postoperative socket healing, pain, swelling and bone regeneration following surgical removal of impacted mandibular third molars. Methods. The present case‒control study was conducted on 20 patients with identical bilateral mandibular third molar impaction. PRP was placed randomly on one side of 3rd molar extraction socket and the contralateral side was used as control. Evaluation of soft tissue healing, pain, swelling and radiologic bone density was carried out. Results. Soft tissue healing was better in the PRP compared to the control site. Immediate postoperative assessment of pain scores showed no significant difference between the two groups (Mann-Whitney U test). On the 7th day, pain scores were lower in case site compared to the control site. Measurement of swelling on the 1st, 3rd and 7th day showed statistically significant differences between the case and control sites (P<0.0001). Postoperative mean bone density at the 3rd and 6th postoperative months was significantly higher in the case site compared to the control site (P=0.00001). Conclusion. The results showed an improvement in wound healing and swelling and an increase in the bone density at PRP site. The growth factors in PRP would improve the hard and soft tissue healing 3 months after molar surgery.

Evaluation of Platelet-Rich Plasma and Neutrophil Antimicrobial Extract as Two Autologous Blood-Derived Agents

The platelet-rich plasma (PRP) and antimicrobial peptides neutrophil extract (AMP extract) were prepared from rabbit neutrophils as two autologous blood-derived preparations, which could be applied locally to enhance healing process of tissues. Both preparations were analyzed using the MALDI TOF method for accurate qualitative assay. Growth factors (PDGF and VEGF) and microbicidal protein were reported in PRP. In AMP extract α-defensins, namely; NP-1, -2, -3a, -3b, -4, and -5 and cathelicidins represented among other by 15-kDa antibacterial protein (p15s) were detected. In the second part of our study the influence of antimicrobial extract on macrophages in vitro was tested. Then, degranulation of neutrophils in vitro and generation of reactive intermediates by these cells under the influence of AMP extract were assessed. As estimated, the addition of AMP extract into cultures of macrophages decreased superoxide anion generation after 5 days of incubation. Furthermore, AMP extract inhibited degranulation and respiratory burst in neutrophils, therefore in this regard it suppress proinflammatory effect of two studied populations of leukocytes.

Clinical evaluation of platelet rich plasma when combined with an alloplastic bone graft material in the treatment of intrabony periodontal defects

Introduction: For periodontal regeneration, various modalities are available; among these,use of growth factors is a developing area for clinicians and researchers, as they stimulate cells responsible for periodontal regeneration. Growth factor is a general term used to denote a class of naturally occurring proteins that function in the body to promote the mitogenesis (proliferation), directed migration, and metabolic activity of cells. Platelet-rich plasma (PRP) is a new application and is a storage vehicle for growth factors, especially platelet-derived growth factor and transforming growth factor-b both of which influence bone regeneration. Aim: The aim of this study was to examine the clinical efficacy of autologous PRP combined with an alloplastic bone graft material (hydroxyapatite and β-tricalcium phosphate [HA/β-TCP], (Ossifi™)) in the treatment of three-wall intrabony periodontal defects. Materials and Methods: Twenty-four patients (15 females and 9 males with age ranging from 28 years to 47 years) with moderate to advanced chronic periodontitis with three-wall intrabony defects were selected for the study. All patients were divided into two groups, i.e., “Group-I” and “Group-II;” 12 patients in each group. “Group-I” was control group with patients treated with HA/β-TCP with saline and “Group-II” was test group entitled to patients who were treated with PRP + HA/β-TCP. Clinical parameters recorded were bleeding on probing, probing depth, and clinical attachment level. Furthermore, the radiographic parameters included depth of intrabony defects, calculated as the difference of distances between cementoenamel junction (CEJ) to the bony defect and CEJ to the alveolar crest. Results and Conclusion: Combination of PRP with alloplastic bone graft material, i.e., test Group-II (PRP + HA/β-TCP), showed a better result than control Group-I (saline + HA/β-TCP) in the treatment of intrabony defects.

Evaluation of platelet-rich plasma and fibrin matrix to assist in healing and repair of rotator cuff injuries: a systematic review and meta-analysis

Objective: To perform a meta-analysis examining the effectiveness of platelet-rich plasma and platelet-rich fibrin matrix for improving healing of rotator cuff injuries. Data sources/design: A meta-analysis of eligible studies was performed after searching Medline, Cochrane, and EMBASE on 14 December 2015. Setting: University hospital. Participants: Patients with rotator cuff injuries. Review methods/intervention: Databases were searched using the keywords “PRP or platelet-rich plasma,” “PRFM or platelet-rich fibrin matrix,” “rotator cuff,” and “platelet-rich” for studies comparing outcomes of patients with rotator cuff injuries that did and did not receive a platelet-rich product. Main measures: The primary outcome was a functional score change from pre- to post-treatment (Scorepost–Scorepre). The secondary outcome was a visual analogue scale (VAS) pain score change from pre- to post-treatment (VASpost–VASpre). Results: A total of 11 studies were included in the meta-analysis. The total number of patients that received platelet-rich plasma or platelet-rich fibrin matrix was 320 and the number of control patients was 318. The standard difference in means of the functional scores was similar between patients administered platelet-rich plasma/fibrin matrix and patients in the control group (standard difference in means for functional scores = 0.029; 95% confidence interval (CI): –0.132 to 0.190; p = 0.725). The standard difference in means was similar between patients administered platelet-rich plasma and the controls (standard difference in means = 0.142; 95% CI: –0.080 to 0.364; p = 0.209). Conclusion: The results of this meta-analysis do not support the use of platelet-rich plasma/platelet-rich fibrin matrix in patients with rotator cuff injuries.

Evaluation of platelet-rich plasma alone or in combination with demineralized freeze dried bone allograft in treatment of periodontal infrabony defects: A comparative clinical trial.

Aims:The use of platelet-rich plasma (PRP) alone in periodontal defects has been controversial and inconclusive. Hence, the present study was designed with the aim to assess the clinical and radiographic effectiveness of PRP alone in infrabony defects.Materials and Methods:Thirty infrabony defects were treated with either autologous PRP with open flap debridement (OFD) or autologous PRP + demineralized freeze dried bone graft (DFDBA) with OFD or OFD alone. Clinical parameters recorded were gingival index, plaque index, probing depth (PD), clinical attachment level (CAL), and gingival recession (REC). Radiographic parameters included defect depth reduction, defect resolution, and crestal bone level. All the parameters were recorded at baseline and 12 months postoperatively.Results:Mean PD reduction and CAL gain were greater in PRP + DFDBA (4.88 ± 1.12 mm and 4.26 ± 1.85 mm) and PRP (4.86 ± 2.12 mm and 4.10 ± 1.47 mm) groups than the control group (2.69 ± 1.37 mm and 1.27 ± 0.89 mm).Conclusions:Within the limits of the study, all the three groups showed significant improvement in clinical parameters from baseline to postoperative 12 months. The amount of defect depth reduction and defect resolution treated with PRP alone group were significantly < PRP + DFDBA. The results pertaining to these parameters were significantly better than the control group.