Evaluation of Platelet-Rich Plasma and Neutrophil Antimicrobial Extract as Two Autologous Blood-Derived Agents

Tomasz Szponder,Anna Smolira,Joanna Wessely-Szponder

doi:10.1007/s13770-017-0035-4

Abstract

The platelet-rich plasma (PRP) and antimicrobial peptides neutrophil extract (AMP extract) were prepared from rabbit neutrophils as two autologous blood-derived preparations, which could be applied locally to enhance healing process of tissues. Both preparations were analyzed using the MALDI TOF method for accurate qualitative assay. Growth factors (PDGF and VEGF) and microbicidal protein were reported in PRP. In AMP extract α-defensins, namely; NP-1, -2, -3a, -3b, -4, and -5 and cathelicidins represented among other by 15-kDa antibacterial protein (p15s) were detected. In the second part of our study the influence of antimicrobial extract on macrophages in vitro was tested. Then, degranulation of neutrophils in vitro and generation of reactive intermediates by these cells under the influence of AMP extract were assessed. As estimated, the addition of AMP extract into cultures of macrophages decreased superoxide anion generation after 5 days of incubation. Furthermore, AMP extract inhibited degranulation and respiratory burst in neutrophils, therefore in this regard it suppress proinflammatory effect of two studied populations of leukocytes.

Highlights

Some naturally occurring substances, so called ‘‘autologous biomaterials’’ have a potential for enhancing the bone and soft tissue healing and help fight infection [1]
The polarization phenotype adopted by a macrophage can have a major influence on healing process, and antimicrobial peptides i.e. defensins and cathelicidins may participate in this regulation [8]
In the second part of our study we evaluated the influence of this antimicrobial peptides (AMP) extract on activity of neutrophils and Mfs in vitro

Summary

Introduction

Called ‘‘autologous biomaterials’’ have a potential for enhancing the bone and soft tissue healing and help fight infection [1]. Antimicrobial peptides are microbicidal molecules and exert many regulatory functions during inflammation, they can act as signalling molecules with potential for modulation of wound repair and inflammation by influence on inflammatory cells [5,6,7]. Among these cells, macrophages (Mfs) initially contribute to the elimination of pathogens and the elicitation of an inflammatory reaction (as M1). The polarization phenotype adopted by a macrophage can have a major influence on healing process, and antimicrobial peptides i.e. defensins and cathelicidins may participate in this regulation [8]

Methods

Results

Conclusion