Euryale Ferox Salisb Research Articles

Hepatoprotective Effect of Seed Coat ofEuryale ferox Extract in Non-alcoholic Fatty Liver Disease Induced by High-fat Diet in Mice by Increasing IRs-1 and Inhibiting CYP2E1.

Non-alcoholic fatty liver disease (NAFLD), a common chronic liver disease characterized by hepatic steatosis, affects 30-40% of the population in the world. The seed of Euryale ferox salisb. possesses several pharmacological actions, including metabolic syndrome. However, the seed coat of E. ferox was usually discarded as waste, which contains comparatively abundant polyphenols, and its biological activity has been rarely investigated. In this work, we evaluate the hepatoprotective effect of E. ferox seed coat extract (EFSCE), in NAFLD mice induced by high-fat diet (HFD). The HPLC-MS analysis indicated that the main components of EFSCE were polyphenols. And then, mice were treated with HFD for 4 weeks to induce NAFLD. The result showed that the body weight, weight of adipose tissue, the ratio of liver to body weight in NAFLD mice increased compared with control group. In addition, blood lipids parameters including total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL) also increased in NAFLD mouse model. It was showed that, after treated with EFSCE (15 and 30 mg/kg/day) for 4 weeks, the body weight, lipids deposition in the liver and blood lipids in HFD-induced NAFLD mice markedly reduced. Compared with NAFLD mice, EFSCE administration could also prevent malondialdehyde (MDA) overproduction and strengthen Superoxide Dismutase (SOD) activity to counteract oxidative stress. Moreover, EFSCE was also found effective in reducing alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity in HFD-induced NAFLD model, which indicated liver injury in NAFLD. Therefore, EFSCE (rich in polyphenols) is indicated as bioactive nature product for HFD-induced NAFLD treatment, by eliminating lipid accumulation and oxidative stress via regulation of IRs-1 and CYP2E1.

Novel pentacyclic triterpene isolated from seeds of Euryale Ferox Salisb. ameliorates diabetes in streptozotocin induced diabetic rats.

The present research was carried out to study the effect of 2β-hydroxybetulinic acid 3β-oleiate (HBAO), a novel compound isolated from the seeds of Euryale ferox salisb. on glycemic control, antioxidant status and histopathological morphological alterations in the liver, pancreas, kidney and heart in streptozotocin induced type-2 diabetes in rats. HBAO was isolated from the seeds of Euryale ferox salisb. according to Lee. Isolation of the active principle HBAO was performed for the first time. To date there are no reports on the isolation and evaluation of 2β-hydroxybetulinic acid 3β-oleiate (HBAO) from Euryale ferox salisb. Assessment of different biochemical parameters like the effect of HBAO on glycemic control, plasma insulin, glycosylated hemoglobin, hepatic glucose-6-phosphate dehydrogenase, glucose-6-phosphatase and fructose-1-6-biphosphatase, hepatic hexokinase, lipid profile, antioxidant marker and histopathology of pancreas, liver and kidney examination was done at the end of the experimentation, i.e. on day 45. HBAO exhibited remarkable improvement in glycemic control, lipid levels, plasma insulin, glycogenic liver enzymes and antioxidant activity in diabetic rats, along with progressive enhancement of distortive histopathological morphology of liver, pancreas and kidney. The results strongly suggest that HBAO could be a potential therapeutic agent in diabetes.

Aquacultural, Nutritional and Therapeutic Biology of Delicious Seeds of Euryale ferox Salisb. : A Minireview.

Euryale ferox Salisb. (Makhana) is a rooted macro-hydrophyte, grown as a crop in north Bihar wetlands, India. Makhana refers to yummy pops prepared through mechanical smacking of the baked seeds of E. ferox. New techniques have been evolved to produce pops from seeds at a brisk pace. It is specially associated with marital rituals possibly in view of its spermatogenic properties. It has a high amino acid index but a low glycemic value. The plant carries medicinal values against cardiovascular and diabetes-related diseases. Makhana pops with a high volume to mass ratio, is developed as a reliable system for drug delivery in the recent times. There is a fair prospect of raising multi-faceted industry based on Makhana culture, harvest, post-harvest safety, preparation of pops and various types of food products therefrom and marketing in areas where water-logging is considered as a curse. It may generate employment and strengthen regional economy propping check on population migration primarily due to non-employment and poverty. Active research is required to harness this macro-hydrophyte along with co-culture of compatible fish varieties and other hydrophytes into a sustainable livelihood and economic prosperity.

Rapid deterioration of wetland in Bangladesh: a scenario of Noikandi Beel

Wetland aggregates at least a part of human heritage. It has direct impact on human culture and society. Besides, wetland comprises significant components in the context of local and national biodiversity. Beel wetland is a unique environment of floral and faunal association. In Bangladesh, wetland plays a vital role in its economic, industrial, ecological, socioeconomic and cultural attitudes. A study was conducted in the Noikandi beel where rapid destruction was observed due to anthropogenic activities. In the present study, seasonality and macro aquatic diversity and of the Noikandi beel Bangladesh was examined. Plant materials were collected from the beel once in a month for a period of 24 months. In the first 12 months, a total of 52 taxa have been recorded. Among them 40.38% was aquatic, 46.77% amphibians and 13.46% were found to be terrestrial. In addition, a rare taxon Euryale ferox Salisb was found. In the consecutive year of the study, 22.03% were aquatic, 52.54% terrestrial, 22.03% amphibians and1.69% was found to be macro algae. The rapid disappearance of aquatic plants is alarming. On the contrary, a good number of plants have been noted that is not associated with wetland environment indicating the transformation of wetland in to terrestrial ecosystem. Along with declining water depth and siltation other anthropogenic activities, agricultural, developmental initiatives have been identified as major threats, towards the Noikandi beel. The recommendations of the present study could be implemented by the government with the help of local people, as well as, this information may be useful for other beels of Bangladesh.J. bio-sci. 24: 33-43, 2016

Transcriptome sequencing and analysis during seed growth and development in Euryale ferox Salisb

BackgroundEuryale ferox Salisb., an annual aquatic plant, is the only species in the genus Euryale in the Nymphaeaceae. Seeds of E. ferox are a nutritious food and also used in traditional Chinese medicine (Qian Shi in Mandarin). The molecular events that occurred during seed development in E. ferox have not yet been characterized. In this study, we performed transcriptomic analysis of four developmental stages (T1, T2, T3, and T4) in E. ferox seeds with three biological replicates per developmental stage to understand the physiological and biochemical processes during E. ferox seeds development.Results313,844,425 clean reads were assembled into 160,107 transcripts and 85,006 unigenes with N50 lengths of 2052 bp and 1399 bp, respectively. The unigenes were annotated using five public databases (NR, COG, Swiss-Prot, KEGG, and GO). In the KEGG database, all of the unigenes were assigned to 127 pathways, of which phenylpropanoid biosynthesis was associated with the synthesis of secondary metabolites during E. ferox seed growth and development. Phenylalanine ammonia-lyase (PAL) as the first key enzyme catalyzed the conversion of phenylalanine to trans-cinnamic acid, then was related to the synthesis of flavonoids, lignins and alkaloid. The expression of PAL1 reached its peak at T3 stage, followed by a slight decrease at T4 stage. Cytochrome P450 (P450), encoded by CYP84A1 (which also called ferulate-5-hydroxylase (F5H) in Arabidopsis), was mainly involved in the biosynthesis of lignins.ConclusionsOur study provides a transcriptomic analysis to better understand the morphological changes and the accumulation of medicinal components during E. ferox seed development. The increasing expression of PAL and P450 encoded genes in phenylpropanoid biosynthesis may promote the maturation of E. ferox seed including size, color, hardness and accumulation of medicinal components.

Ethnic uses of Makhana (Euryale ferox Salisb.) in Mithila (north Bihar) and other parts of India

The paper provides a report on the ethnic uses of Makhana (Euryale ferox Salisb.) in different parts of India including Mithila area of North Bihar that is known for being a site of its organized cultivation over centuries. In major parts of northern India E. ferox is available in wild or semi-wild forms. A survey on the ethnic uses of Makhana in Mithila and other parts of India has revealed its about a dozen distinct uses. These include its specific association with (i) ‘Kojaagaraa’ (a marital ritual) (ii) ‘Parikramaa’ (for maintaining counts during ritualistic circumambulation around a temple or tree) (iii) ‘Shraaddha Karma’ (last Hindu rites) (iv) Devotional offering to deities or distinguished persons in the form of non-flower garlands (v) ‘Prasaad’ (edible offering to deities) (vi) Sacred thread ‘Yajnopaveet’ as stiffening item (vii) ‘Makhaan Paagal’ i.e., caramelised pops (during Navratri and other festivals). It is used as a ‘Havan Saamagri’ and as a component of ‘Panchamevaa’ (i.e., five auspicious dry fruits). ‘Ghunes’, a head gear ceremonially worn by the bridegroom on the occasion of wedding has streams of Makhana pops hanging downward. This practice is observed by the Srotriyas in this area. The paper further incorporates its other traditional and modern uses mostly in the form of culinary preparations. These are also in practice in other areas of the country and abroad in the form of (i) 2 forms of snacks (ii) Dessert (iii) Sweetened Makhana (iv) Porridge (v) Dal Makhani (vi) Makhana Kofta and (vii) Makhana Dum Aloo.

Extract of Euryale ferox Salisb exerts antidepressant effects and regulates autophagy through the adenosine monophosphate-activated protein kinase-UNC-51-like kinase 1 pathway.

Positive regulation of autophagy by Euryale ferox Salisb (ES) leads to antidepressant effects. This study focused on the potential antidepressant mechanisms induced by the petroleum ether fraction of ES (ES-PE) in the chronically unpredictable mild stress (CUMS) mouse model. Deficits in the sucrose preference test, open field test, tail suspension test, and forced swim test were observed following CUMS, and were reversed following ES-PE administration. Contrary to the expression of mammalian target of rapamycin, autophagy was decreased after establishment of the CUMS model. We also observed trends for downregulation of adenosine mononphosphate-activated protein kinase (AMPK) and mammalian autophagy-initiating kinase (ULK1), which were differentially affected by ES-PE. HT22 cells and Compound C, an inhibitor of AMPK, were used to verify the results in mice. Gas chromatography-mass spectrometry analysis revealed high level of vitamin E acetate in ES-PE. Taken together, our data indicate that ES-PE activated autophagy by regulating the AMPK pathway. © 2018 IUBMB Life, 70(4):300-309, 2018.

An Investigation of the Prescription Patterns of Chinese Herbal Products for Chronic Glomerulonephritis Patients: A Hospital-Based Cross-Sectional Study.

Chronic kidney disease (CKD) has a high incidence and prevalence worldwide, and chronic glomerulonephritis (CGN) is one of the main causes of CKD. Therefore, it is important to diagnose and treat CGN early. The purpose of this study is to analyze the prescription patterns and frequencies of Chinese herbal products (CHPs) for CGN by using a hospital-based database from the Chang Gung Memorial Hospital (CGMH), a large, tertiary hospital system in Taiwan, and to evaluate the safety and possible efficacy of CHPs by blood test. The International Classification of Disease Ninth Revision (ICD-9) code 582 was used to identify patients with CGN. From 2004 to 2015, a total of 54726 CHP prescriptions for CGN were provided. Association rule mining was used to analyze the prevalent of CHP combination patterns in treating CGN. Jia-Wei-Xiao-Yao-San (JWXYS) and Gorgon (Euryale feroxSalisb.) were the most frequently prescribed herbal formula (HF) and single herb (SH), respectively. The most frequently prescribed combination of CHPs was that of JWXYS with Bu-Yang-Huan-Wu-Tang (BYHWT) in CGMH. In statistical, the level of eGFR in Stage 3a and 3b group was increasing after treatment in 6 and 12 months and might not cause the renal function to worsen within 12-month treatments. To the best of our knowledge, this is the first pharmacoepidemiological study to review CHP treatments for CGN. However, additional studies and clinical trials are needed to provide data on the safety and efficacy of these CHPs.

The hypoglycemic and antioxidant effects of polysaccharides from the petioles and pedicels of Euryale ferox Salisb. on alloxan-induced hyperglycemic mice.

The present study investigated the potential hypoglycemic and antioxidant effects of polysaccharides extracted from the petioles and pedicels of Euryale ferox Salisb. (EFPP) on alloxan-induced hyperglycemic mice. The EFPP had a total carbohydrate of 65.72 ± 2.81%, uronic acid of 4.56 ± 0.62% and protein of 0.58 ± 0.12%, with an average molecular weight from 1.02 kDa to 11.45 kDa and monosaccharide composition of Man, GlcA, Rha, Glc, Gal and Ara at a molar ratio of 0.12 : 0.01 : 9.57 : 0.41 : 1.00 : 0.24. Administration with EFPP, especially high dose EFPP, was beneficial to reverse body weight loss, reduce blood glucose levels, enhance serum insulin levels, improve oral glucose tolerance, increase hepatic glycogen content and GCK activity, and modulate the mRNA expression of GCK in the liver. Meanwhile, EFPP had protective effects against alloxan-induced oxidative injury in mice, via increasing the activities of SOD, CAT and GSH-Px and decreasing the MDA contents in the liver and kidney of the mice. EFPP ameliorated the damage in pancreas, kidney and liver tissues, which was confirmed by histopathological observation. The results suggested that EFPP possess hypoglycemic and antioxidant activities, and could be a potential source of natural hypoglycemic and antioxidant agents for functional foods or complementary medicines.

Nutritional status of recently developed Makhana (Gorgon Nut) variety “Swarna Vaidehi”

Makhana is an important aquatic, annual and seed propagated crop with gigantic floating leaves. It is known as Gorgon nut (Euryale ferox Salisb) belongs to family Nympheaceae. Swarna Vaidehi has been released as an ever first variety of makhana originated from selection-6. Nutritional profiling was undertaken for popped seeds of Swarna Vaidehi and local cultivar along with raw seeds of “Swarna Vaidehi”. Standard procedure was applied for nutritional profiling of the Makhana samples. Results reveled that maximum moisture content (34.7%) %) was recorded in case of raw seeds of swarna vaidehi. Maximum ash content (0.4%) was recorded in case popped seeds of both tested samples i.e. swarna vaidehi, lowest value (0.3%). Maximum seed protein (8.7%) was obtained by the swarna vaidehi. Maximum crude fiber (0.5%) was obtained in the raw seeds of swarna vaidehi as compare to popped one. Maximum total carbohydrate (79.8%) was recorded in popped seeds of swarna vaidehi; however the lowest value (57.0%) was also noticed in raw seeds of swarna vaidehi. It worth to notice that maximum calorific value (358) was recorded in popped seeds of swarna vaidehi. Maximum (18.5mg) calcium content was recorded in case of popped seeds of swarna vaidehi. Likewise maximum (1.3) manganese content was recorded in case of popped seeds of swarna vaidehi. It was worth to mention that maximum (1.1) zinc content was recorded in case of popped seeds of swarna vaidehi.

정원 연못내 가시연꽃(Euryale ferox Salisbury) 도입을 위한 기초연구 II - 토양과 수환경을 중심으로 -

본 연구는 연못내 가시연꽃 도입을 위한 일련의 연구로, '식물상과 식생환경'에 이어 '토양과 수환경' 기초자료 획득을 목적으로 한 후속연구이다. 전라북도 내의 가시연꽃 자생지 14곳에 대한 문헌 및 현장조사를 통해 자생지의 토양환경과 수문환경에 대한 분석을 통해 얻은 결과는 다음과 같다. 최소한 저토와 수환경의 부영양화는 가시연꽃 생육의 장애가 되지는 않다고 판단되며 약 70년 이상의 지령(池齡)을 갖는 서식지에서 가시연꽃 생육이 왕성한 것으로 파악된다. 가시연꽃 생육지의 토성은 점토 80.2%, 미사 16.7%, 모래 3.1%로 '중식토(重埴土)'로 분류된다. 토양(저니)의 유기물 함량은 평균 36g/kg으로 가시연꽃 생육지와 비생육지 사이에 주목할 만한 차이는 없었다. 한편 가시연꽃 자생지의 수질 환경은 pH 6.5~7.9, 용존산소 농도 <TEX>$1.8{\sim}8.8mg/{\ell}$</TEX>, COD 농도 <TEX>$6.8{\sim}74mg/{\ell}$</TEX>, 부유물질 <TEX>$2.0{\sim}213mg/{\ell}$</TEX>, 총질소 <TEX>$0.422{\sim}10.723mg/{\ell}$</TEX>, 인산염 인 <TEX>$0.003{\sim}0.126mg/{\ell}$</TEX>로 나타났다. 가시연꽃의 녹피율과 활력도가 높은 김제 명덕지, 임실 대정저수지 그리고 정읍 애당저수지의 평균 용존산소(DO) 농도는 <TEX>$3.5mg/{\ell}$</TEX>, 총질소 농도 <TEX>$1.33mg/{\ell}$</TEX>, 인산염 인 <TEX>$0.06mg/{\ell}$</TEX>로 조사되었다. 이를 전체 평균값과 비교해보면 DO농도와 총질소 농도는 다소 낮고 인산염 인의 농도는 2배 이상 높은 것으로 추후 인산염 인의 농도와 가시연의 활력도의 상관관계에 대한 심도 있는 연구가 필요하다. 지금까지 확인된 본 연구 결과를 가시연꽃 관상을 목적으로 하는 정원 연못 조성의 기초자료로 제시한다. Through the research and analysis on the hydrological environment and soil environment of habitats through documentary and field studies over 14 habitats of Euryale ferox Salisb. within Jeollabukdo, with the objective of acquiring the basic data for forming an environment based on plantation of reservoirs that are composed with Euryale ferox, the following results were obtained. As a result of analyzing the construction period of the habitats of Euryale ferox from a total of 14 places, the average period of duration after construction of all subject reservoirs appeared to be 71.8 years. Moreover, when examining the relationship between the age of reservoirs and eutrophication, it could be judged that at least the eutrophication of subsoil and water environment is not an obstacle to the growth of Euryale ferox grows in habitats that have a reservoir age of approximately 70 years or more. As a result of analyzing the gardening of soil sediment of the Euryale ferox habitats, the component ingredients appeared to be composed of 80.2% of clay, 16.7% of silt and 3.1% of sand, and the soil class pursuant to such was classified as 'heavy clay'. The organic matter contents of soil sediment appeared to be an average of 36g/kg, and there appeared to be no noticeable difference between the habitats and non-habitats of Euryale ferox. The water quality environment of Euryale ferox habitat appeared to be pH 6.5~7.9, concentration of dissolved oxygen to be <TEX>$1.8{\sim}8.8mg/{\ell}$</TEX>, concentration of COD to be <TEX>$6.8{\sim}74mg/{\ell}$</TEX>, floating materials to be <TEX>$2.0{\sim}213mg/{\ell}$</TEX>, total nitrogen to be <TEX>$0.422{\sim}10.723mg/{\ell}$</TEX>, and phosphate to be <TEX>$0.003{\sim}0.126mg/{\ell}$</TEX>. The average DO concentration of Aedang Reservoir at Jeongeup, Daejeong Reservoir at Imsil, and Myeongdeokji at Gimje with high vitality and green coverage ratio of Euryale ferox appeared to be <TEX>$3.5mg/{\ell}$</TEX>, total nitrogen to be <TEX>$1.33mg/{\ell}$</TEX>, and concentration of phosphorus-phosphate to be <TEX>$0.061mg/{\ell}$</TEX>. When comparing such with the entire average value, the DO and total nitrogen concentration appeared to be rather low, and the phosphorus-phosphate concentration appeared to be higher by two times or more, thus, an in-depth study on the correlation of the vitality of Euryale ferox Salisb. and concentration of phosphorate-phosphorus will be needed in the future.

Relative importance of hydrological variables in predicting the habitat suitability ofEuryale feroxSalisb.

Aquatic ecosystems are a priority for conservation as they have become rapidly degraded with land-use changes. Predicting the habitat range of an endangered species provides crucial information for biodiversity conservation in such rapidly changing environments. However, the complex network structure of aquatic ecosystems restricts spatial prediction variables and has hitherto limited the use of habitat models to predict species occurrence in aquatic ecosystems. We used the maximum entropy model to evaluate the potential distribution of an endangered aquatic species, Euryale ferox Salisb. We tested the relative influence of (i) climatic variables, (ii) topographic variables, and (iii) hydrological variables derived from remote sensing data to improve the prediction of occurrence of aquatic plant species. We considered the southern part of the Korean Peninsula as the modeling extent for the potential distribution of E. ferox. Occurrence records for E. ferox were collected from the literature and field surveys. We applied maximum entropy modeling using remotely sensed environmental variables and evaluated their relative importance as prediction variables with variation partitioning. The species distribution model predicted potential habitats of E. ferox that matched the actual distribution well. Floodplain wetlands and shallow reservoirs were the favored habitats of E. ferox. Quantitative loss and fragmentation of wetland habitats appeared to be a major reason for the decrease of E. ferox populations. Our results also imply that hydrological variables (i.e. normalized difference water index) derived from remote sensing data greatly increased model prediction (relative contribution: 10.5–37.0%) in the aquatic ecosystem. However, interspecific competition within a similar niche environment should be considered to increase the accuracy of the distribution model.

Antidiabetic, antioxidant, antihyperlipidemic effect of extract of Euryale ferox salisb. with enhanced histopathology of pancreas, liver and kidney in streptozotocin induced diabetic rats.

BackgroundEthanolic extract of Euryale ferox salisb. (EFx) may have an effect on the activity of hepatic antioxidant enzymes, glycemic control and lipid profile and histopathology of pancreas, liver and kidney of streptozotocin (STZ)—induced diabetic wistar rats.MethodsWistar albino rats were divided into eight groups viz. non-diabetic (normal control), diabetic control (STZ-induced), diabetic treated (infused with different doses of Euryale ferox. Salisb. ethanolic extract) and diabetic conventional treated (treated with Glibenclamide). Diabetes was induced by administering streptozotocin (60 mg/kg body weight) intraperitoneal (i.p). The ethanolic extract was supplemented in different doses through oral route. Biochemical investigations were carried out according to previously reported methods. Histopathological examinations were done accordingly.ResultsThe EFx supplemented diabetic rats significantly (p < 0.001) decreased the blood glucose level in a dose dependent manner. Plasma insulin level was significantly increased in EFx treated rats. The hepatic gluconeogenic enzymes activities were restored to normal in EFx treated rats. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) were significantly increased (p < 0.001) among EFx treated rats. Lipid profile was reinstated to nearly normal level among EFx treated rats. Histopathological investigations revealed that microscopic architecture of pancreatic, hepatic and renal cells improvised in EFx treated diabetic rats.ConclusionEFx supplement could improve the glycemic control as well as lipid profile in diabetic rats along with improvised antioxidant enzymes which has beneficial effect in preventing the diabetic complications by scavenging the free radicals in diabetic rats.

Simultaneous Determination of 16 Nucleosides and Nucleobases in Euryale ferox Salisb. by Liquid Chromatography Coupled with Electro Spray Ionization Tandem Triple Quadrupole Mass Spectrometry (HPLC-ESI-TQ-MS/MS) in Multiple Reaction Monitoring (MRM) Mode.

In this study, a simple, rapid, efficient analytical method was established for the qualification and quantification of 16 nucleosides and nucleobases in Euryale ferox Salisb. by using liquid chromatography coupled with electrospray ionization tandem triple quadrupole mass spectrometry (HPLC-ESI-TQ-MS/MS) in multiple-reaction monitoring (MRM) mode. Ideal separation of 16 target compounds was achieved on Xbridge Amide HILIC column (4.6 × 150 mm, 3.5 μm) with gradient elution in 11 min by optimized conditions. Variations of nucleosides and nucleobase in samples from different cultivation regions ranging from 190.50 to 1594.30 μg/g were obvious. The total nucleoside contents were higher than total nucleobases, especially in the compositions of guanosine, cytidine and 2'-deoxyguanosine. Samples 1-18 with dense thorns were better characters than samples 19-26 without thorns in terms of nucleosides and nucleobases concentrations in general. The limits of detection (LODs) and quantification (LOQs) for 16 analytical substances were investigated to be 0.11-6.33 ng/mL and 0.35-21.1 ng/mL, respectively. And the method was first applied to large aquatic plants with good linearity, precision, repeatability and accuracy. All present information provided a scientific and rational reference for quality assessment and control of Euryale ferox Salisb.

2β-hydroxybetulinic acid 3β-caprylate: an active principle from Euryale Ferox Salisb. seeds with antidiabetic, antioxidant, pancreas & hepatoprotective potential in streptozotocin induced diabetic rats.

The aim of the present study was to evaluate the glycemic control, antioxidant, pancreas and liver protective effect of 2β-hydroxybetulinic acid 3β-caprylate (HBAC) from Euryale ferox Salisb. seeds on streptozotocin induced diabetic rats. The active principle was isolated from Euryale ferox Salisb. seeds extract by utilizing chromatographic techniques. The rats were divided into seven experimental groups: Gp 1-normal; Gp2- normal + HBAC (60mg/kg p.o.); Gp3- diabetic control; Gp 4- Diabetic + HBAC (20mg/kg p.o.); Gp5- Diabetic + HBAC (40mg/kg p.o.); Gp6- Diabetic + HBAC (60mg/kg p.o.) and Gp 7- Diabetic + Glibenclamide (10mg/kg p.o.). Biochemical estimation, free radical scavenging examination and histopathological study was performed at the end of experimentation i.e. on 28th day. The active principle isolated and identified with spectral data as 2β-hydroxybetulinic acid 3β-caprylate (HBAC). It was detected for the first time that HBAC has improvised the glycemic control in streptozotocin induced diabetic rats. Furthermore, it is remarkable to note that it exhibited excellent free radical scavenging property and pancreas and hepatoprotective property as well, supported by histopathological examination. One of the mechanisms of action of HBAC appears to be stimulating the release of insulin from pancreatic β-cells. HBAC improved the glycemic control, reduced the free radical activity along with corrected glycemic control, lipid profile, and enhanced level of insulin alongh with improvement in pancreas and hepatoprotective architecture. Considering the above results, HBAC shows potential to develop a medicine for diabetes as combinatorial or mono-therapy.

Seed dormancy and germination traits of an endangered aquatic plant species, Euryale ferox Salisb. (Nymphaeaceae)

Populations of Euryale ferox Salisb. have declined throughout its global range because of habitat loss and degradation. The present study aimed to evaluate the influence of storage temperature (4 and 20°C), storage period (0, 90, 180, 270, and 360 days), light condition (light and darkness), germination temperature (constant 10, 15, 20, 25, and 30°C), and seed size (two classes from 0.5cm to less than 1.2cm, and from 1.2cm to less than 1.6cm) on germination of E. ferox seeds. Our results indicated that seeds were dormant when shed and 4°C stratification promoted germination more effectively than 20°C. After stratification at 4°C, the germination frequency of the larger seeds peaked at 90 days’ stratification, whereas the smaller seeds maintained high germination frequencies up to 180 days’ stratification. The different germination responses between smaller and larger seeds may reflect a difference in the rate of induction of dormancy in the annual dormancy cycle. Seeds germinated in both light and darkness, which demonstrated that light is not involved in the regulation of E. ferox seed germination. The optimal temperature for germination was 25°C. Light condition×germination temperature interaction caused significantly higher germination frequency at 30°C in light than in darkness, and the opposite trend at 15°C.

Makhana (Euryale ferox Salisb.) - cum - fish culture: An integrated management for better yield

Integration of makhana (Euryale ferox Salisb.)-cum-fish culture including sole system as sub-plot on the nutritional quality under wetland ecosystem reveal that seed yield as well as nutritional value of makhana seeds (starch, sugar, protein and minerals) vary significantly by different integrated nutrient management (INM) packages applied on the crop and fishes. The result exhibited highest value (75.04, 2.37, 9.45 and 0.52%) with N4 treatment (plants and fishes both received a well-balanced organic and inorganic sources of plant nutrients along with fish-feed including zinc spray). The combined effect of different farming system and INM on the nutritional status of makhana seeds was not significant. These important food ingredients as fresh as well as its different value-added products have great importance for small and cottage industries, particularly poor and marginal section of rural people as well. DOI: http://dx.doi.org/10.3329/bjsir.v48i4.11501 Bangladesh J. Sci. Ind. Res. 48(4), 281-286, 2013

Extraction optimization, isolation, preliminary structural characterization and antioxidant activities of the cell wall polysaccharides in the petioles and pedicels of Chinese herbal medicine Qian (Euryale ferox Salisb.)

Cell wall polysaccharides in the petioles and pedicels of Qian (Euryale ferox Salisb.) (EFPP) were extracted using ultrasound-assisted technique. Response surface methodology (RSM) based on Box–Behnken design (BBD) was employed to optimize extraction parameters for the maximum purity of polysaccharides. The results showed that the optimum extraction conditions were extraction temperature of 80°C, extraction time of 32min, ultrasonic power of 270W and liquid-to-solid ratio of 40mL/g. Under the optimal conditions, the experimental purity of polysaccharides was 62.57%±1.68%, which was very close to the predicted. The crude EFPP were isolated using DEAE-52 column and four major fractions (EFPP-1, EFPP-2, EFPP-3 and EFPP-4) were obtained. Typical functional groups of polysaccharides were characteristic for EFPP-1, EFPP-3 and EFPP-4 from FT-IR spectrum. Furthermore, the crude EFPP and three fractions (EFPP-1, EFPP-3 and EFPP-4) possessed appreciable in vitro antioxidant effects on α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,2′-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), hydroxyl radical scavenging and reducing powers. Then, the crude EFPP and EFPP-4 could effective against H2O2-induced injury on HUVEC and VSMC through enhancement of T-AOC, SOD and CAT activities and decrease of MDA content.

Antioxidant and anti-fatigue activities of phenolic extract from the seed coat of Euryale ferox Salisb. and identification of three phenolic compounds by LC-ESI-MS/MS.

This study investigated the antioxidant potential and anti-fatigue effects of phenolics extracted from the seed coat of Euryale ferox Salisb. The in vitro antioxidant potentials, including scavenging DPPH, hydroxyl radical activities and reducing power were evaluated. Antioxidant status in vivo was analyzed by SOD, CAT, GSH-Px activities and the MDA content in liver and kidneys of D-galactose-induced aging mice. The anti-fatigue effect was evaluated using an exhaustive swimming test, along with the determination of LDH, BUN and HG content. The phenolic extract possessed notable antioxidant effects on DPPH, hydroxyl radical scavenging and reducing power. The mice which received the phenolic extract showed significant increases of SOD, CAT (except for in the kidney), GSH-Px activities, and a decrease of MDA content. The average exhaustive swimming time was obviously prolonged. Meanwhile, increase of LDH content and decrease of BUN content were observed after mice had been swimming for 15 min. The HG storage of mice was improved in the high and middle dose extract groups compared with the normal group. The contents of total phenols and gallic acid of the extract were determined. Three compounds in the extract were identified as 5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-chroman-4-one, 5,7,4-trihydroxyflavanone and buddlenol E. These results suggest that the extract of E. ferox is a promising source of natural antioxidants and anti-fatigue material for use in functional foods and medicines.

Неотипификация Euryale ferox Salisb. (Nymphaeaceae)

Our analysis of the protologue and inquires in the Herbaria of the UK, namely Kew and the British Museum, revealed the absence of the type of Euryale ferox Salisb. in their collections. The neotype of E. ferox is designated due to co-operation with foreign colleagues.