Antidiabetic, antioxidant, antihyperlipidemic effect of extract of Euryale ferox salisb. with enhanced histopathology of pancreas, liver and kidney in streptozotocin induced diabetic rats.

Danish Ahmed,Manju Sharma,Amita Verma,Vikas Kumar,Girija Shankar Shukla

doi:10.1186/s40064-015-1059-7

Abstract

BackgroundEthanolic extract of Euryale ferox salisb. (EFx) may have an effect on the activity of hepatic antioxidant enzymes, glycemic control and lipid profile and histopathology of pancreas, liver and kidney of streptozotocin (STZ)—induced diabetic wistar rats.MethodsWistar albino rats were divided into eight groups viz. non-diabetic (normal control), diabetic control (STZ-induced), diabetic treated (infused with different doses of Euryale ferox. Salisb. ethanolic extract) and diabetic conventional treated (treated with Glibenclamide). Diabetes was induced by administering streptozotocin (60 mg/kg body weight) intraperitoneal (i.p). The ethanolic extract was supplemented in different doses through oral route. Biochemical investigations were carried out according to previously reported methods. Histopathological examinations were done accordingly.ResultsThe EFx supplemented diabetic rats significantly (p < 0.001) decreased the blood glucose level in a dose dependent manner. Plasma insulin level was significantly increased in EFx treated rats. The hepatic gluconeogenic enzymes activities were restored to normal in EFx treated rats. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) were significantly increased (p < 0.001) among EFx treated rats. Lipid profile was reinstated to nearly normal level among EFx treated rats. Histopathological investigations revealed that microscopic architecture of pancreatic, hepatic and renal cells improvised in EFx treated diabetic rats.ConclusionEFx supplement could improve the glycemic control as well as lipid profile in diabetic rats along with improvised antioxidant enzymes which has beneficial effect in preventing the diabetic complications by scavenging the free radicals in diabetic rats.

Highlights

Ethanolic extract of Euryale ferox salisb. (EFx) may have an effect on the activity of hepatic antioxidant enzymes, glycemic control and lipid profile and histopathology of pancreas, liver and kidney of streptozotocin (STZ)— induced diabetic wistar rats
There are several evidences that demonstrate that diabetic complications are closely associated with oxidative stress persuaded by free radicals generation (Pitkänen et al 1992) Type-2 diabetes mellitus (T2DM) is a multifunctional disease which is associated with hyperglycemia and lipoprotein abnormalities (Ugochukwu and Babady 2002)
Ferox salisb. seeds, we aimed to investigate the plausible role of EFx on STZ-induced diabetic rats

Summary

Introduction

Ethanolic extract of Euryale ferox salisb. (EFx) may have an effect on the activity of hepatic antioxidant enzymes, glycemic control and lipid profile and histopathology of pancreas, liver and kidney of streptozotocin (STZ)— induced diabetic wistar rats. There are several evidences that demonstrate that diabetic complications are closely associated with oxidative stress persuaded by free radicals generation (Pitkänen et al 1992) Type-2 diabetes mellitus (T2DM) is a multifunctional disease which is associated with hyperglycemia and lipoprotein abnormalities (Ugochukwu and Babady 2002). Free radicals formed during diabetic state, serves an important role in the genesis of diabetic complications (Ahmed et al 2014a). Antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) provides an important assistance in scavenging the free radicals by breaking down the ROS. Prior researches have reported that a defect in the ROS scavenging machinery in T2DM patients as compared to the normal control (Ahmed et al 2013)

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