Ethyl Biodiesel Research Articles

Stirring and mixing in ethylic biodiesel production

This study aimed to analyze the influence of stirring and mixing on the production of soybean biodiesel. It was verified the influence of the baffle, different stirring speeds and two types of impellers (turbine and blade). The experiments were conducted by full experimental design 23 with the following fixed parameters: temperature (70 °C), oil/ethanol molar ratio (1/10), catalyst amount (1.5%), and reaction time (30 min). The independent variables were impeller type (turbine or blade), baffle (presence or absence), and stirring speed (150 or 350 rpm). Chromatographic analyses helped to determine the yield of biodiesel production at different operating times. The optimized parameters turbine impeller, absence of baffle, and stirring speed of 350 rpm provided high biodiesel yields within the first minutes of reaction. The analysis of the effect over time allows to verify in which period the variables had more influence on the biodiesel production and in which time there is no increase of the yield.

Evaluating Antioxidant Eugenol on Oxidation Stability of Biodiesel Synthesized from Used Frying Oil

Due the environmental appeal, the demand of new processes and inputs has been increasing considerably in the last decade. Biodiesel is a biodegradable fuel obtained from vegetable oils or animal fats, including those discarded after use. The methyl and ethyl transesterification routes for biodiesel production were tested by using residual frying oil as raw material through the homogeneous basic catalyst, such as sodium hydroxide. In present work, it was evaluated the efficiency of the eugenol on the oxidation stability of the methyl and ethyl biodiesels synthesized from residual frying oil. The chromatographic analysis determined that the conversion of the yield to the methyl and ethylic esters (biodiesel) was 90.6 % and 87.3 %(m/m) respectively. For both biodiesel samples a rise of the eugenol concentration added resulted in an increase of the induction period (oxidation stability parameter evaluated by Rancimat method), with a consequent reduction of the acid number. Taking these results into account, it can be concluded that the samples obtained after eugenol addition can be used as biodiesel. DOI: http://dx.doi.org/10.17807/orbital.v10i1.1030

Comparing the environmental impacts of ethyl biodiesel production from soybean oil and beef tallow through lca for brazilian conditions

The present paper sought compare the environmental impacts throughout the life cycle of biodiesel production obtained from the two raw materials most used in Brazil (soybean oil and beef tallow) through the process ethyl transesterification in an alkaline medium. The reference flow adopted for the work was the generation of power supplied 1GJ from the produced biodiesel. The data used in the inventory life cycle were calculated based on similar scientific papers. The method of assessment of environmental impacts chosen was the CML 2001 modified. Altogether, it were analyzed nine categories of environmental impacts for both processes (abiotic depletion (kg Sb eq), land use (m2a), global warming (kg CO2 eq), ozone layer depletion (kg CFC-11 eq), human toxicity (kg 1,4-DB eq), freshwater ecotoxicity (kg 1,4-DB eq), terrestrial ecotoxicity (kg 1,4-DB eq), acidification (kg SO2 eq) and eutrophication (kg PO43- eq)). The results of evaluation of environmental impacts show that the biodiesel production process from soybean oil presents major environmental damage in seven categories of analyzed impacts (destruction of abiotic resources, destruction of the ozone layer, human toxicity, freshwater ecotoxicity, terrestrial ecotoxicity, acidification and eutrophication). The production process of biodiesel from tallow presents major environmental damage in two categories of impacts analyzed (land use and global warming). However, the results show that the absolute values of environmental damage caused by impacts of the production process using beef tallow are much more aggressive.

Deacidification and ethyl biodiesel production from acid soybean oil using a strong anion exchange resin

The objective of this work was to investigate simultaneously the deacidification and the ethyl biodiesel production from soybean oil using the Amberlyst A26 OH anion exchange resin. The behavior of the process was investigated as a function of resin loading, ethanol concentration and initial free fatty acids (FFAs) content. To obtain the acidified oil, degummed soybean oil and commercial linoleic acid were mixed. The Langmuir model was able to better describe the FFAs adsorption isotherms in comparison to the Freundlich equation. A high removal of FFAs with high deacidification rate could be obtained using lower initial ratios of FFAs per mass of dry resin, being this the more favorable condition for oil deacidification. Ester formation showed to be affected by the resin loading, initial FFA content and ethanol concentration in the medium. The adsorption of FFAs seemed to deactivate the catalytic activity of the resin sites. For this reason, a high resin loading was required, although it was still possible to obtain a satisfactory biodiesel production. The present work makes possible deeper understanding of oil processing by anion exchange resins in the presence of an alcoholic solvent.

Chamotte clay as potential low cost adsorbent to be used in the palm kernel biodiesel purification

Solid waste from the red ceramic industry (chamotte clay) was assessed as glycerol adsorbent for biodiesel purification by dry washing. Its structure and composition was analyzed by several techniques, such as X-ray diffraction, FT-IR, scanning electron microscopy and particle size. By using a simulated medium (5w/v% glycerol in an ethanol solution), a face centered composite design was used to analyze the combined effect of chamotte concentration and temperature on glycerol removal. Chamotte levels of 2.5w/v% at 45°C maximized glycerol removal (1282mgglycerolg−1) with minimum loss of adsorbate. Kinetic data on glycerol adsorption by chamotte clay could be adjusted using the pseudo-second order model, which indicates that the rate-limiting step may be chemisorption. Moreover, chamotte clay was used for purifying ethyl biodiesel samples from palm kernel oil by chemical and biochemical catalysis. In the presence of chamotte clay, low glycerol levels (<0.02wt%) were found in comparison with crude biodiesel (0.29±0.03wt%). Chamotte clay was also able to remove residual levels of monoacylglycerols and diacylglycerols and provided samples with properties that are similar to those attained by traditional wet washing.

Study ecotoxicity of biodiesel from residual oils and fats and the effects of salinity aquatic ecosystems

The increasing use of biodiesel raised concerns about its toxicological effects on the environment. However, few studies have analyzed the impact of biodiesel, especially ethyl biodiesel from residual frying oils and fats (RFOF), on aquatic organisms. Here, were used biodiesel produced by acid esterification followed the alkali transesterification and biodiesel produced by saponification, acidification and two acids esterification, both in the laboratory and their mixtures (B100, B20, and B7). The eluates were obtained by mixing biodiesel with saline water and tested the effect of several concentrations of them on the mortality rate of nauplii of the brine shrimp Artemia salina. The results of study showed that blend of diesel with greater proportion of biodiesel caused less mortality rate to A. salina nauplii. The saline eluate with 93% diesel and 7 % biodiesel (E4 and E10) showed the mortality of A. salina on 50% eluate exposure while E7 it was 90%. The mixture does increase toxicity with the presence of diesel, B100, only in E8 mortality occurred (LD50 = 100%). Conversely, the eluates ER1/100 and ER2/100 produced from ethyl biodiesel of RFOF R1 and R2 biodiesel, respectively, did not have toxicity.

Crystallization behaviors and rheological properties of biodiesel derived from methanol and ethanol

Take palm oil and soybean oil biodiesels as example, crystallization behavior and rheological property of methyl and ethyl biodiesels were investigated. Differential scanning calorimeter (DSC) technique was used to analyze the crystallization behaviors of methyl and ethyl biodiesels, including the crystallization onset temperature and the variation of amount of crystal precipitation with temperature. Meanwhile, a thermodynamic model of regular solution was proposed and compared with the crystallization experiments. Comparisons suggested that the model can give a reasonable description of crystal precipitation of biodiesel. The rheological properties of methyl and ethyl biodiesels including the viscosity temperature curves as well as the variation of viscoelastic parameters with temperature during the cooling gelation process were measured by rheometer. Experimental results suggested that the crystallization onset temperature and the gelation temperature of ethyl biodiesels are decreased as compared with methyl biodiesels. When temperature is just below the crystallization onset temperature of methyl biodiesel, the crystal precipitation of ethyl biodiesel is much lower than methyl biodiesel, meanwhile, the same characteristic can be observed for the viscosity and storage modulus of methyl and ethyl biodiesels. As the temperature decreased, the crystal precipitation of ethyl biodiesel is gradually close to until equal to the methyl biodiesel. So as the temperature decreased, the differences between the viscosity, storage modulus of methyl and ethyl biodiesels are getting smaller, and then the viscosity and storage modulus of ethyl biodiesel are greater than those of methyl biodiesel.

Phase equilibrium data and modeling of ethylic biodiesel, with application to a non-edible vegetable oil

Contributing to extending the knowledge for the design and operation of biodiesel production processes, isobaric PTxy vapor-liquid equilibria data of ethanol+ethyl hexanoate, 1-pentanol+ethyl hexanoate and 1-pentanol+ethyl octanoate at two different pressures are reported for the first time. Consistency tests were applied to attest the quality of the collected data, for these especially complex measurements. Besides that, vapor pressures of the pure ethyl esters have also been measured. For modeling purposes, the Lyngby and Dortmund UNIFAC variants were used to predict the VLE phase diagrams. Generally, the predictions are of very good quality, being the UNIFAC-Do (Dortmund) better, as the deviations in temperature and vapor compositions are always lower to 1.0K and 0.020, respectively. Checking for the viability for extrapolations in pressure, CPA EoS was also applied to the modeling of the experimental data with very good results. Finally, aiming at examining the model capabilities to describe multicomponent systems, VLE measurements involving two alcohols and the fatty acid ethyl ester mixture obtained from non-edible vegetable oil have been carried out showing the good performance of the predictive models.

Anaerobic Biodegradation of Ethylic and Methylic Biodiesel and Their Impact on Benzene Degradation

The aim of the present work was to study the anaerobic biodegradation of benzene in the presence of ethylic and methylic biodiesel. Biodiesel was produced by methanolysis and ethanolysis of virgin sunflower oil and ethanolysis of waste frying oil, and further characterized according to the European Biodiesel Standard EN 14214 for water content, fatty acid esters content, acid value, kinematic viscosity at 40°C, and oxidation stability at 110°C. Microcosms were set up in order to assess the biodegradation of the different biodiesel types and their impacts on benzene biodegradation. Methane production and benzene biodegradation were monitored for 85 days. Denaturing gradient gel electrophoresis was used to assess impacts on archaeal and bacterial community fingerprints. The results showed that the three types of biodiesel were readily biodegraded, even in the presence of benzene. In microcosms with only biodiesel, the highest biodegradation rate and extent was observed for sunflower methylic biodiesel followed by sunflower ethylic biodiesel and waste frying oil ethylic biodiesel. Similar results were observed in the presence of benzene. The presence of any biodiesel type negatively impacted benzene biodegradation with soybean ethylic biodiesel impacting rates the least. Microbial community fingerprint analyses showed that biodiesel composition selected distinct communities according to the alcohol employed in the transesterification reaction.

The thermal processing in air of ethylic soybean biodiesel after accelerated aging, with and without antioxidant

Biodiesel is highly susceptible to oxidative degradation during storage or when submitted to thermal processing in air, which is influenced by the composition and the physical–chemical properties of the raw materials, from which it was originated. This work evaluates the influence of synthetic commercial antioxidants on the oxidative degradation of ethylic soybean biodiesel after being submitted to accelerated aging. Biodiesel–antioxidant blends were prepared using a concentration of 500 mg kg−1 of the following commercial antioxidants: 2,6-di-tert-butyl-4-metyl phenol (Ph); 2,2′-methylene-bis (4-methyl-6-tert-butylphenol) (BPh); N,N′-di-sec-butyl-p-phenylenediamine + 2,6-di-tert-butylphenol (PA); and 2-tert-butylhydroquinone (HCA). Samples with and without antioxidant were held for 30, 60 and 90 days in an oven at 43 °C, to accelerate their aging process (ASTM D 4625-04), after which, they were analyzed by thermogravimetry in air, and DSC with real-time view (DSC-RV). The present paper discusses the main volatilization and thermal decomposition steps that occur during the analyses, quantifying the still non-degraded components, as well as the residual carbonaceous products that are formed during the accelerated aging processes or during analysis. HCA and BPh antioxidants showed a more effective action in the maintenance of the biodiesel oxidative stability than Ph and PA antioxidants. The higher was the biodiesel oxidative degradation during aging, the higher was the formation of high molecular weight products, which were quantified by thermogravimetry.

Evaluation of different aging procedures on biodiesel thermal degradation process

The sediments that form during biodiesel oxidation decrease the ignition quality, damaging the injector nozzle, the filter and the motor operation system. They may form in a very fast way, and their correct quantification is still a problem. The insoluble oxidation products due mainly to biodiesel degradation in diesel/biodiesel blends may be characterized by several analytical techniques. The conventional methods currently used for sediment quantification are gravimetric, but do not present repetitive results, still presenting a technical challenge to be solved. In this work, the thermal degradation process of a commercial soybean ethylic biodiesel (BS100) was evaluated by thermogravimetry, derivative thermogravimetry and by infrared spectroscopy, after being submitted to different aging procedures. Analyses of samples aged at 43 °C, 110 °C and 150 °C were compared to the original non-aged sample. As diesel engines operate under oxidizing conditions, thermal analyses were performed with synthetic air and the results were compared with the analyses in nitrogen.

Ethyl biodiesels derived from non-edible oils within the biorefinery concept – Pilot scale production & engine emissions

Procedures and operating conditions optimized in laboratory scale for the production of ethyl biodiesels from non-edible vegetable oils (NEVOs) were successfully transferred at pilot scale, with implementation of separation and purification stages. The three NEVOs candidates are Balanites aegyptiaca (BA), Azadirachta indica (AI), and Jatropha curcas (JC), converted into BAEEs, AIEEs and JCEEs respectively via homogeneous catalysis. Quality specifications of the produced biofuels were used to explain pollutant emissions and engine performance observed via a power generator. Under the same conditions, blends of petrodiesel with crude BA or JC oil (50 wt.%) were also investigated.The selected overall methodology “feedstock-conversion-engine” led to the proposal of a sustainable alternative fuel. The candidate NEVO is BA oil to which the proposed alkali route should lead to a low cost biodiesel production process thanks to easy operating conditions, associated with a two-stage procedure (glycerol recycling) and a dry-purification method (rice husk ashes). Glycerol addition should be carried out at ambient temperature to play positively at phenomena occurring in the reacting medium (chemical kinetics, chemical equilibrium, phase equilibrium). Tests on power generator demonstrated that BAEEs led to cleaner combustion than petrodiesel, particularly for the most harmful emissions (light carbonyls and ultrafine particulate matter).

Biodiesel production from bitter almond oil as new non-edible oil feedstock

ABSTRACTBitter almond (Prunus dulcis var. amara) seed oil was tested as new non-edible feedstock for producing ethylic biodiesel by base-catalyzed transesterification with ethanol. The oil was extracted from bitter almond seed with maximum yield of 42.0 wt%, which can be considered as significant feedstock for biodiesel production. The experimental parameters involved in the optimization process were the type and concentration of the catalyst, ethanol-to-oil molar ratio, reaction temperature, reaction time, and rate of stirring. The maximum yield of biodiesel (97.1% w/w) was obtained using 0.60 wt% KOH, 7:1 ethanol-to-oil molar ratio, 65°C reaction temperature, 60 min of reaction, and 700 rpm rate of stirring. The fuel properties of the biodiesel were within the acceptable limits prescribed by ASTM D 6751. The 1H NMR spectroscopy assured the conversion of bitter almond oil into biodiesel. Based on these results, it was concluded that bitter almond seed oil is an acceptable non-edible feedstock for biodiesel production.

Development of Energetic Alternatives to Use of Waste of Annona muricata L.

Two new types of biodiesel were assessed, produced by transesterification of the oil from Annona muricata L. seeds, using basic catalysis with methanol or ethanol, obtaining yields of 91 and 83%, respectively. The values of esters showed that the conversion of the oil into biodiesel was quite high 95.5 and 95.2% for methyl and ethyl biodiesel, respectively, this shows that the transesterification reaction was efficient producing a quality biodiesel. Both types were analyzed according to the parameters set by Brazil’s National Petroleum, Natural Gas and Biofuels Agency (ANP), responsible for overseeing the quality and sale of fuels in the country. These analyses demonstrated that both new products can be used as fuels. No significant quality differences were observed between them. The new biofuels were also assessed when blended with petroleum diesel at different concentrations (up to 30%), presenting excellent results with respect to the parameters set by the ANP.

Kinetic of the formation of short-chain carboxylic acids during the induced oxidation of different lipid samples using ion chromatography

Short-chain carboxylic acids are commonly formed in the oxidation of vegetable oils, long carbon-chain fatty acids and biodiesel. The formation of short-chain (up to six carbons) carboxylic acids during induced oxidation in the Rancimat procedure for oleic acid (OA), linoleic acid (LA), linolenic acid (LnA), soybean oil (SO), methyl biodiesel from soybean oil (MBSO) and ethyl biodiesel from soybean oil (EBSO) was studied. Samples of OA, LA and LnA were submitted to oxidation, and the short-chain carboxylic acids formed were identified in their anionic forms using ion chromatography (IC). Formate, acetate, valerate and hexanoate were identified in the OA and LA samples. For the LnA sample formate, acetate, valerate, propionate and butyrate were found. For the MBSO, EBSO and SO, formate, acetate, propionate, valerate and hexanoate were identified. The rate of formation of formic acid was highest for each sample. The quantitative analysis of short-chain carboxylic acids present in lipid samples can help in determining product age, degree of oxidation, oxidative stability and corrosiveness.

Prática de sustentabilidade na administração pública: reaproveitamento de óleo de fritura para produção de biodiesel

O presente trabalho teve como objetivo produzir biodiesel a partir de óleo residual de fritura, coletado por meio de campanha educativa promovida no Campus Ipojuca do Instituto Federal de Educação, Ciência e Tecnologia de Pernambuco (IFPE), como uma prática de sustentabilidade na esfera pública federal. Diversos órgãos governamentais começaram a estabelecer ações voltadas a sustentabilidade e racionalização dos gastos e processos somente depois da criação da Instrução Normativa n° 10/2012, que os obrigou a criar um Plano de Gestão de Logística Sustentável (PLS). No entanto, poucas ações tem sido efetivadas no que diz respeito ao reaproveitamento do óleo de fritura. Assim, torna-se necessário buscar soluções para a reciclagem de resíduos gerados pela Administração Pública, em especial aqueles que representam riscos de poluição ambiental. Sabe-se que o óleo de fritura usado polui o ambiente quando descartado incorretamente, sendo a produção de biodiesel uma das formas mais nobres de reaproveitamento do óleo residual. O biodiesel é um biocombustível alternativo ao diesel de petróleo, podendo ser obtido a partir da reação de transesterificação entre triglicerídeos e alcoóis de cadeia curta, na presença de um catalisador enzimático, ácido ou alcalino, sendo a glicerina um coproduto. Neste estudo, amostras de biodiesel foram produzidas no Campus Ipojuca do IFPE, utilizando catálise básica (KOH e NaOH) em rotas metílica e etílica. Foi realizado um estudo sobre as variáveis do processo de conversão do óleo em biodiesel (como tipos de reagentes, tempo e temperatura da reação) para a obtenção de uma metodologia apropriada para produção de biodiesel em escala laboratorial. Para o óleo residual, foram determinados o índice de acidez, pH, teor de água, densidade e teor de ácidos graxos livres (AGL) e comparados com amostra de óleo virgem de milho e valores da literatura. Algumas propriedades das amostras de biodiesel produzidas foram comparadas com os limites estabelecidos na Resolução n° 45/2014, da Agência Nacional de Petróleo, Gás e Biocombustíveis (ANP). Como resultado, verificou-se que o óleo residual apresenta diferentes características físico-químicas em relação ao óleo virgem, decorrentes da degradação oxidativa induzida pelo processo de cocção dos alimentos. O maior rendimento da reação de transesterificação foi obtido pela rota metílica (93%) em comparação à rota etílica (76%), como esperado. Os valores experimentais de densidade e o teor de água obtidos para o biodiesel metílico e etílico estão de acordo com os parâmetros da ANP, porém a acidez encontrada para o biodiesel metílico supera em 8% o limite máximo de 0,5 mg KOH/g estabelecido na legislação, tornando necessário o aprimoramento das etapas de lavagem e neutralização para correção de pH e diminuição do índice de acidez do biodiesel. No teste de chama, as amostras de biodiesel produzidas apresentaram uma queima semelhante ao diesel comercial e biodiesel de algodão (produzido em Caetés-PE), demonstrando a viabilidade técnica da reciclagem de óleo de fritura para produção de biocombustível como uma prática de sustentabilidade em uma instituição pública federal voltada para o desenvolvimento local sustentável em todas as suas dimensões.

EXPERIMENTAL DATA, THERMODYNAMIC MODELING AND SENSITIVITY ANALYSES FOR THE PURIFICATION STEPS OF ETHYL BIODIESEL FROM FODDER RADISH OIL PRODUCTION

Abstract - The goals of this work were to present original liquid-liquid equilibrium data of the system containing glycerol + ethanol + ethyl biodiesel from fodder radish oil, including the individual distribution of each ethyl ester; to adjust binary parameters of the NRTL; to compare NRTL and UNIFAC-Dortmund in the LLE representation of the system containing glycerol; to simulate different mixer/settler flowsheets for biodiesel purification, evaluating the ratio water/biodiesel used. In thermodynamic modeling, the deviations between experimental data and calculated values were 0.97% and 3.6%, respectively, using NRTL and UNIFAC-Dortmund. After transesterification, with 3 moles of excess ethanol, removal of this component until a content equal to 0.08 before an ideal settling step allows a glycerol content lower than 0.02% in the ester-rich phase. Removal of ethanol, glycerol and water from biodiesel can be performed with countercurrent mixer/settler, using 0.27% of water in relation to the ester amount in the feed stream.

Mixed metal oxides from sucrose and cornstarch templated hydrotalcite-like LDHs as catalysts for ethyl biodiesel synthesis

Sucrose and cornstarch were used as organic templates in the synthesis of hydrotalcite-like layered double hydroxides (LDHs) prepared by the coprecipitation with partial replacement of Mg by Zn, and Al by Fe. Non-template materials were taken as references. All LDHs were used as precursors of mixed metal oxides (MMOs) by calcination and applied as catalysts for ethyl biodiesel synthesis via transesterification reactions of soybean oil and ethanol. The LDHs were characterized by XRD, TG, FTIR-ATR and the respective MMOs by SEM, BET, basicity and acidity. Crystallographic data from templated LDHs showed lower crystallinity compared to the reference homologous materials, and it was observed that cornstarch decreases the average surface areas (from 173 to 94m2g−1) and the size of the crystallites (from 147 to 92Å). FTIR spectra indicated carbohydrates were occluded inside the crystals. While this did not influence the number of acidic sites on the MMOs, it enhances the alkaline sites. Under the catalytic conditions of this experiment, cornstarch templated MMOs reach an average ethyl biodiesel conversion of 70% whereas those prepared with sucrose 45%, and the homologous reference materials only 36%. These results demonstrated the great potential of using carbohydrates as biotemplates in the preparation of heterogeneous catalysts for transesterification reactions.

Monitoring of biodiesel content and adulterant presence in methyl and ethyl biodiesels of jatropha in blends with mineral diesel using MIR spectrometry and multivariate control charts

In this work, fast and efficient analytical methodologies were developed for quality monitoring of the content of jatropha methyl and ethyl biodiesels in blends with mineral diesel, and to monitor the presence of adulterants using mid-infrared (MIR) spectrometry allied to the method of multivariate control charts based on net analyte signal (NAS). The methodology of multivariate control charts consists of dividing the spectral signal of calibration samples into three vectors, namely NAS, interference and residual, generating three charts. The NAS chart monitors the quality of the analyte of interest in blends (biodiesel content, in this case), while the interference chart monitors the diesel quality, i.e., monitors the presence of adulterants or contaminants in the binary mixtures. The residual chart monitors the information or variations not modeled by the NAS and interference vectors. The validation of control charts in both models was done in two ways: (i) in relation to the biodiesel content in the biodiesel/diesel blends and (ii) in relation to the presence of contaminants in blends. In relation to the biodiesel content, sets of samples with biodiesel content in and out of control of the quality specifications were prepared for validation. In relation to the presence of adulterants, validation was performed by applying other sets of samples adulterated by the partial substitution of biodiesel in the blends by soybean oil and used frying oil (UFO) in the range of 0–30%, and by partial substitution of diesel by residual automotive lubricant oil (RALO) and gasoline, also in the range of 0–30%. The results of the application of multivariate control charts demonstrated satisfactory monitoring for the identification of samples of B7 blends in or out of quality specifications and identification of the presence of adulterants in blends. That is, the proposed methodologies correctly identified samples as in- or out-of-control. Therefore, the methodologies were shown to be effective for use by the supervisory board of such blends of these biofuels.

Ethylic Biodiesel Production Using Lipase Immobilized in Silk Fibroin-Alginate Spheres by Encapsulation

The Amano AK lipase from Pseudomonas fluorescens was immobilized in fibroin-alginate spheres (FA-LPf) and used to catalyze the transesterification reaction of soybean oil under different conditions (solvents, time, amounts of immobilized lipase, and temperature) for the production of biodiesel (fatty acid ethyl esters, FAEE). The FAEE was quantified by GC-FID analysis, and tricaprin was used as an internal standard. The maximum FAEE yield (63%) was attained after 96 h at 32 °C and 400 rpm in 30% (v/v) n-hexane, which used 20% (w/w) of the immobilized lipase. However, it is noteworthy that with a period of 96 h, the spheres of FA-LPf were partially destroyed by friction from the magnetic stirring, and the long time for the transesterification reactions is impractical in these conditions. For this reason, the reaction time was reduced to 48 h, which resulted in 42% FAEE and assures better reuse conditions for four consecutive cycles. This study shows the efficacy of an enzyme immobilized in a biodegradable FA-LPf support.