Ethanol Extract Of Plant Research Articles

ISOLATION, CHARACTERIZATION OF Β-SITOSTEROL FROM THE HERBAL EXTRACTS AND EVALUATION OF IN VITRO ANTI-OXIDANT AND ANTI-DIABETIC ACTIVITIES

Plants naturally produce phytochemicals, physiologically active chemical compounds that serve as a natural defence mechanism and have considerable potential for treating a wide range of ailments. Cyperus rotundus and Tinospora cordifolia are the potent traditional herbs used in Ayurveda medicine, especially for diabetes. The current study aimed to isolate and characterize the bioactive compound β-sitosterol from the combined ethanolic extracts of the rhizome of Cyperus rotundus (C. rotundus) and the stem of Tinospora cordifolia (T. cordifolia). Furthermore, the in vitro antioxidant and anti-diabetic activities of isolated compounds are tested by DPPH assay, alpha amylase inhibition assay, alpha glucosidase inhibition assay and glucose uptake activity in 3T3L1 cells. The combined ethanolic extract of both medicinal plants was initially subjected to column and preparative thin layer chromatography for the isolation of bioactive compounds, followed by their characterization by spectral methods like UV, FT-IR, 1H and 13C NMR. The structure of the isolated compound is identified as β-sitosterol and further confirmed by structural elucidation. Similarly, the isolated compound β-sitosterol exhibited higher antioxidant scavenging activity of 64.54±1.38 µg/ml against DPPH at a concentration 100µg/ml. The study also confirmed that β-sitosterol showed good inhibitory activities towards alpha-amylase (62.21±1.25 µg/ml) and alpha-glucosidase (65.48±1.47 µg/ml). β-sitosterol showed significantly increased glucose uptake (28% at 100μg/ml). Based on the conclusion that the organic compound β-sitosterol exhibited good antioxidant and anti-diabetic efficacy, it could have been used in the treatment of free radical disease and can be utilized in the management of diabetes mellitus.

Biogenic Copper Nanoparticles from combined leaf extracts of Catharanthus roseus (L.) and Abutilon indicum(L.) and their biological effects – an In vitro study

Plant-based (Green) synthesis of Copper nanoparticles is of much interest to researchers currently because of its important applications in various fields of biotechnology. In this study, the production of copper nanoparticles (CuNPs) utilizing an ethanolic extract of Catharanthus roseus (L.) and Abutilon indicum (L.) leaves extract (CALE) and assess the therapeutical use of these nanoparticles. Preliminary phytochemical analysis was done by using this ethanolic crude extract of the combined plant. The phytochemical tests showed positive results for the phytochemicals alkaloids, phenols, tannins, terpenoids, and phytosterols. The leaf extract was used for the synthesis of copper nanoparticles and in the reduction and capping of the combined leaf extract CuNPs, it was discovered that the bioactive phytoconstituents condensed tannins, played a crucial role by the reduction of copper ions was identified by the change in color from blue to light green. SEM image showed the morphology and size of the nanoparticles and their binding ability with the combined leaf extracts. The spherical biogenic combined leaf extracts of CuNP’s were measured to have a size between 5 and 25 nm by Scanning Electron Microscopy (SEM) examination, Ultraviolet-Visible spectroscopy (UV-Vis) showed maximum wavelength at 224nm and XRD showed three strong peaks for its crystallinity and maximum was observed at 2063.0. From their characterization of synthesized CuNPs anti-microbial and anti-inflammatory methods were assessed. The samples showed different diameters of zone of inhibition at different concentrations by using Ampicillin as positive control. The synthesized nanoparticles showed a concentration-dependent anti-inflammatory activity, and the protection percentage increased with an increase in the concentration of the samples.

Biogenic Copper Nanoparticles from Combined leaf extracts of Catharanthus roseus (L.) and Abutilon Indicum(L.) and their biological effects – an In vitro study

Plant-based (Green) synthesis of Copper nanoparticles is of much interest to researchers currently because of its important applications in various fields of biotechnology. In this study, the production of copper nanoparticles (CuNPs) utilizing an ethanolic extract of Catharanthus roseus (L.) and Abutilon indicum (L.) leaves extract (CALE) and assess the therapeutical use of these nanoparticles. Preliminary phytochemical analysis was done by using this ethanolic crude extract of the combined plant. The phytochemical tests showed positive results for the phytochemicals alkaloids, phenols, tannins, terpenoids, and phytosterols. The leaf extract was used for the synthesis of copper nanoparticles and in the reduction and capping of the combined leaf extract CuNPs, it was discovered that the bioactive phytoconstituents condensed tannins, played a crucial role by the reduction of copper ions was identified by the change in color from blue to light green. SEM image showed the morphology and size of the nanoparticles and their binding ability with the combined leaf extracts. The spherical biogenic combined leaf extracts of CuNP’s were measured to have a size between 5 and 25 nm by Scanning Electron Microscopy (SEM) examination, Ultraviolet-Visible spectroscopy (UV-Vis) showed maximum wavelength at 224nm and XRD showed three strong peaks for its crystallinity and maximum was observed at 2063.0. From their characterization of synthesized CuNPs anti-microbial and anti-inflammatory methods were assessed. The samples showed different diameters of zone of inhibition at different concentrations by using Ampicillin as positive control. The synthesized nanoparticles showed a concentration-dependent anti-inflammatory activity, and the protection percentage increased with an increase in the concentration of the samples.

In Silico Exploration and Experimental Validation of Camellia sinensis Extract against Rhipicephalus microplus and Sarcoptes scabiei: An Integrated Approach.

Sarcoptes scabiei is an ectoparasite of humans and animals that causes scabies. The Rhipicephalus (Boophilus) microplus is a blood-sucking ectoparasite that transmits various pathogens. These two parasites have caused great losses to a country's dairy and agriculture sectors. The aim of this study was to determine the in vitro and in silico efficacy of Camellia sinensis plant extracts. Different concentrations of C. sinensis ethanolic plant extracts were prepared using the maceration method and were used against mites and ticks (in adult immersion test AIT and larval packet test LPT) to evaluate their in vitro acaricidal activity. Additionally, in silico molecular docking was performed to investigate the inhibitory interactions between the phytochemicals of the plant and S. scabiei and R. microplus glutathione transferase proteins (SsGST and RmGST). This study observed that the plant extract showed high efficacy in vitro against mites and different tick stages in adult immersion and larval packet tests. Additionally, the in silico study revealed a strong binding interaction between ellagic acid and SsGST protein, with a binding energy of -7.3 kcal/mol, with respect to permethrin (-6.7 kcal/mol), whereas quercetin and RmGST resulted in a docking score of -8.6 kcal/mol compared to deltamethrin (-8.2 kcal/mol). Overall, this study explored the potential of C. sinensis as a natural alternative for controlling tick and mite infestations and provided insights into the inhibitory mechanisms of its phytochemicals.

Novel acaricidal and growth-regulating activity of Aloe vera and Rheum rhabarbarum extracts and their oil/water nanoemulsions against the camel tick, Hyalomma dromedarii

Hyalomma dromedarii is an important tick species infesting livestock. This work evaluated the novel adulticidal, insect growth-regulating, and enzymatic efficacy of ethanol plant extracts of Aloe vera and Rheum rhabarbarum and their nanoemulsions against males and engorged females of the camel tick, H. dromedarii. The physicochemical properties of nanoemulsions were evaluated. The High-Performance Liquid Chromatography (HPLC) analyses indicated that the extracts contained polyphenols and flavonoids, which could enhance their acaricidal effect. Dynamic light scattering (DLS) of the nanoemulsions of A. vera and R. rhabarbarum were 196.7 and 291 nm, whereas their zeta potentials were − 29.1 and − 53.1 mV, respectively. Transmission electron microscope (TEM) indicated that nanoemulsions showed a regular spherical shape (less than 100 nm). Fifteen days post-treatment (PT) with 25%, the mortality% of A. vera and R. rhabarbarum were 88.5 and 96.2%, respectively. Five days PT, the median lethal concentration values of A. vera, R. rhabarbarum, and their nanoemulsions were 7.8, 7.1, 2.8, and 1.02%, respectively, and their toxicity indices were 91.02, 100, 36.4, and 100%, respectively. Their median lethal time values PT with 3.5% were 6.09, 5.09, 1.75, and 1.34 days, respectively. Nanoemulsions enhanced the efficacy of the crude extract 1–7 folds, 5 days PT, and accelerated their speed of killing ticks 2–4 times. The total protein and carbohydrates, Acetylcholinesterase, Alpha esterase, and Amylase were affected PT. The reproductive potential of engorged females was adversely impacted. In conclusion, the novel A. vera and R. rhabarbarum extracts were promising acaricides, and their nanoformulations enhanced their efficacies.

Bazı etanolik bitki ekstrelerinin histon deasetilaz inhibitör ve antioksidan aktiviteleri: Kanser için yeni bir terapötik yaklaşım

Purpose: The aim of this study was to evaluate histone deacetylase inhibitory and antioxidant activities of some ethanolic plant extracts. 
 Materials and Methods: In this study, the inhibitory effects of ethyl alcohol extracts prepared from various plants and some chemical compounds on histone deacetylase activity were investigated. In addition, the antioxidant activity of plant-derived active substances, which have had an important value in alternative medicine in recent years, has been determined to replace chemicals.
 Results: All the plant extracts and chemical substances used in the study showed histone deacetylase inhibitory effect (with IC50 range of 0.000078-319.0 µg/mL) and antioxidant activity. The results indicated that the percentage of histone deacetylase inhibition and antioxidant activities increased with increasing concentrations of the plant extracts and chemical compounds. 
 Conclusion: These plant extracts which are potential sources of histone deacetylase inhibitors may be appropriately used as an alternative support to drug treatment rather than the use of toxic chemicals.

Validating Interactions of Pathogenic Proteins of Staphylococcus aureus and E. coli with Phytochemicals of Ziziphus jujube and Acacia nilotica.

This study focused on the assessment of the antimicrobial resistance of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) isolated from bovine mastitis milk samples and the revealing anti-mastitis potential of phytocompounds of Ziziphus jujube and Acacia nilotica through molecular docking analysis. The mastitis milk samples were collected from various dairy farms for the isolation of the bacteria (S. aureus and E. coli) and their response to antibiotics. Ethanolic extracts of both plants were prepared. Their antibacterial activity was evaluated, and they were processed for phytochemical analysis after which, molecular docking analysis with pathogenic proteins of the bacteria was carried out. Parametric and non-parametric statistical analyses were performed to reach the conclusions of this study. The findings of the study revealed a higher drug resistance (≥40%) of E. coli against ampicillin, amikacin, and vancomycin, while S. aureus exhibited the highest resistance to ampicillin, erythromycin, and ciprofloxacin. The ethanolic extracts of the Ziziphus jujube and Acacia nilotica plants produced a ZOI between 18 and 23 mm against multidrug-resistant S. aureus and E. coli. Gas chromatography-mass spectrophotometry (GC-MS) was used to explore 15 phytocompounds from Ziziphus jujube and 18 phytocompounds from Acacia nilotica. The molecular docking analysis of 2cyclopenten-1-one,3,4,4 trimethyl and Bis (2ethylhexyl) phthalate of Ziziphus jujube showed a binding affinity of -4.8 kcal/mol and -5.3 kcal/mol and -5.9 kcal/mol and -7.1 kcal/mol against the DNA Gyrase and toxic shock syndrome toxin-1 proteins of S. aureus and E. coli, respectively. The suberic acid monomethyl ester of Acacia nilotica showed a binding affinity of -5.9 kcal/mol and -5 kcal/mol against the outer membrane protein A and Topoisomerase IV protein of E. coli and -5.1 kcal/mol and -5.8 kcal/mol against the toxic shock syndrome toxin-1 and Enterotoxin B proteins of S. aureus. Similarly, 2,2,4-trimethyl-1,3-pentanediol di-iso-butyrate showed a binding affinity of -6.5 kcal/mol and -5.3 kcal/mol against the outer membrane protein A and Topoisomerase IV of E. coli and -5.2 kcal/mol and -5.9 kcal/mol against the toxic shock syndrome toxin-1 and Enterotoxin B proteins of S. aureus, respectively. The study concluded that there was an increasing trend for the antimicrobial resistance of S. aureus and E. coli, while the Ziziphus jujube and Acacia nilotica plant extracts expressed significant affinity to tackle this resistance; hence, this calls for the development of novel evidence-based therapeutics.

Evaluation the efficacy of Ethanol Extract Derived from Wild Mint on the Life Parameters of the Aphis fabae (Hemiptera: Aphididae) (Sco-poli) under laboratory conditions

The black bean aphid, Aphis fabae is a significant economic pest with a global presence, impacting a wide range of plant species. This study aimed to assess the effectiveness of ethanol extract derived from wild mint (Mentha longifolia) under laboratory conditions on various life performance criteria of A. fabae. The evaluation involved measuring mortality rates of nymphs and adults, as well as adult productivity, utilizing three different extract concentrations (1%, 2%, and 3% w/v). The results revealed that the ethanol extract of plant leaves had a substantial effect on all life stages of the aphid, with a more pronounced impact observed at higher concentrations and longer exposure periods. In the first nymphal instar, the mortality rate reached 81% at a concentration of 3%, and in the second nymphal instar, it was 80% at the same concentration. For the third and fourth nymphal instars, the mortality rates were 82% and 82.5%, respectively, at a concentration of 3%. As for adult aphids, the mortality rate was 81.333% at a concentration of 3%. Moreover, the extract significantly reduced the productivity of adult aphids, with rates of 1.68%, 1.82%, and 1% at concentrations of 1%, 2%, and 3%, respectively.

Neuroprotective evaluation of Marsilea quadrifolia L. and Salvinia molesta D.S Mitchel in aluminium chloride induced Alzheimer disease

Phenolic compounds are very effective for the cure of various neurological and pathological diseases. Current study was conducted to estimate the Phenolic compounds through HPLC and to perform the anti-Alzheimer activity from two aquatic Leptosporangiate ferns; Marsilea quadrifolia L. and Salvinia molesta D. Crushed leaves were subjected to Microwave Assisted Extraction to prepare plant leave ethanolic extract of plants under study. Aluminum chloride induced Alzheimer’s disease model in rats by estimating behavioural and biochemical parameters were measured for ethanolic extract of both plants. Different behavioural tests were performed for neuromuscular coordination estimation and hole board test for exploratory behaviour were performed. The levels of AChE inhibitory activity and antioxidant enzymes were estimated on brain homogenates and Histopathological analysis were also performed. Phenolic compounds of leaf extracts were noted for HPLC chromatograms of standard compounds with Gallic acid (33.76 ± 0.08) higher in M. quadrifolia L. and Salicylic acid (12.45 ± 0.07) higher in S. molesta D. S Mitchel. Histopathological analysis showed that M. quadrifolia and S. molesta reversed the neurofibrillary tangles, degenerative changes, neuronal loss and neuroinflammation in the brain architecture. Both ferns at a dose of 500 mg/kg and 1000mg/kg significantly improved the Aluminium chloride induced neurotoxicity by modulating cognitive disabilities and motor dysfunctions, and normalized the biochemical alteration of acetylcholinesterase and antioxidant enzymes. In conclusion, both plants M. quadrifolia and S. molesta improved behavioural and biochemical parameters and therefore will be effective in treatment of neurodegenerative disorders via countless underlying mechanistic pathways.

Total phenolic, flavonoid, protein contents and biological activities of wild mustard

AbstractThe nutritional and therapeutic benefits of plants are outstanding. Wild mustard (Sinapis arvensis L.) collected from different regions was analysed for its phenolic, flavonoid, protein, and biological activity levels. In this case, the plant ethanol extract was obtained using a soxhlet apparatus. Rel assay kits and the DPPH test were used to assess the plant's antioxidant activity. The agar dilution test was used to determine antimicrobial efficacy. A549 lung cancer cells were used in an MTT assay to measure antiproliferative activity. The Folin−Ciocalteu reagent was used to quantify the total phenolic content of the sample. The amount of flavonoids was determined using an aluminium chloride test. The amount of protein was calculated using the AOAC's standard technique. Based on the research conducted, it was shown that the maximum total antioxidant status (TAS) value for the ethanol extract of wild mustard from various places was 5.232 ± 0.047 mmol L−1. At a concentration of 2 mg mL−1, DPPH activity was measured to be 82.06 ± 1.01. The maximum levels of total phenolic, flavonoid, and protein were 80.57 ± 2.19 mg g−1, 154.07 ± 2.79 mg g−1, and 7.75 ± 0.24%, respectively. Doses of 25–100 μg mL−1 of plant extracts were effective against fungal strains, whereas doses of 50–200 μg mL−1 were beneficial against bacterial strains. The plant extracts were shown to have potent antiproliferative properties. It was found that wild mustard's phenolic, flavonoid, protein, and biological activity levels varied according to the location from which it was gathered. It was also concluded that wild mustard had significant biological activity.

Antimicrobial properties of ethanolic plant extracts on microorganisms of the genus Staphylococcus aureus, Echerihia coli

Nowadays, the importance of medicinal plants has increased significantly as a source of biologically active substances, including antibacterial action. There is an intensive search for effective and safe drugs that could perform the function of preventive disinfection, reduce the negative impact on the animal's body and the environment as a whole, and be economically beneficial. Therefore, we chose 17 promising types of plant raw materials for the study due to the sufficient prevalence and diversity of chemical composition. The scientific work presents the results of the effectiveness of ethanolic plant extracts against aureus, Echerihia coli and Staphylococcus. The antibacterial effect of plant tinctures of Kalgan, Comfrey, Bearberry, Geranium, Nut membranes and Dandelion on St. Aureus strain 209 P and E. coli strain 1257 was revealed. According to the results of the studies, high and moderate antibacterial activity of plant materials was found, which is an effective means of preventive measures in veterinary medicine. It has also been found that the “wells” method is more effective than the paper disc method and gives a more accurate result, so it is relevant in further research. Herbal medicines account for more than 50 % of all pharmaceuticals. A significant place among them is occupied by prophylactic disinfectants used to prevent diseases. The main active substances of such products are secondary metabolites of medicinal plants, which include phenols and flavonoids, that in turn have antimicrobial effects. The content of these substances is controlled by analyzing extracts made from plant material. Calgan is a perennial medicinal plant of the Pink family that has a number of powerful properties. The rhizome of this plant contains flavonoids, wax, iron, starch, malic acid, essential oils, catechin, glycosides, vitamins PP, A, Z. Comfrey is a perennial herbaceous plant that belongs to the family of broadleaves. The highest concentration of nutrients is found in the rhizome of the three-year-old plant: tannins, amino acids, alkaloids, flavonoids, etc. Geranium is a perennial herb of the geranium family, rich in tannins – tannins are found in the rhizome and ground part of the plant. It also contains flavonoids, triterpene saponins, carotene, vitamin C, and starch. Dandelion is a perennial plant from the aster family. The roots contain inulin, fructose, organic acids, steroids, flavonoids, tannins, waxes, and higher fatty acids. The walnut membranes are rich in iodine, zinc, magnesium, ascorbic and nicotinic acids, PP and B vitamins, tannins, and essential oils. Bearberry is a genus of evergreen hardwood shrubs of the heather family. The main active ingredients are phenolic compounds.

Effect of ethanol and aqueous extract of Moringa oleifera on bacteria isolated from wound infection

The aqueous and ethanol extracts of Moringa oleifera used for the treatment of infectious disease were tested for their active against gram positive and gram-negative bacteria isolated from burns infection culture using the broth dilution and disc diffusion method. Results of this study revealed the presence of phytochemical which were active against gram positive and negative bacteria. Ethanol extracts of plant showed highest activity other than aqueous extract. The minimum inhibitory concentration (MIC) of the aqueous extracts on the test organism was 25- 100 mg\ml, while that of the ethanol extract ranged between 25 -50 mg\ml on the test organisms, the minimum bacterial concentration (MBC) ranging between 25-100 mg\ml for ethanol extract, and 25-200 mg\ml for aqueous extracts. The highest activity at 45 oC was demonstrated by the ethanol extracts of S. rosmarinus against Staphylococcus aureus and Klebsiella spp. In this study plant extracts against gram negative bacteria showed activity in acidic pH only in contrast of gram positive bacteria which were constant in plant extract. M. oleifera contained essential elements at higher levels. The results of this study suggest the possibility of using the ethanolic extracts of plant in treating diseases caused by the test organisms, especially when prepared at acidic pH.

Antimalarial activity test and GC-MS analysis of ethanol and ethyl acetate extract of snake plant (Sansevieria trifasciata Prain)

Snake plant or Mother-in-law's tongue (Sansevieria trifasciata Prain) is a plant possessing of many ethnobotanical uses one of which is for medicinal. Previous pharmacological studies showed that this plant has several properties such as healing lesions, anthelmenthic, antimicrobial and cytotoxic. This research was conducted to investigate phytocompounds constituents of ethanol and ethyl acetate of S. trifasciata and their antimalarial activity potential. The phytochemical constituents were analyzed using Gas Cromatography and Mass Spectroscopy (GCMS). The antiplasmodial properties of the extract against malaria parasites were determined by calculating the percentage of parasitaemia suppression which was then used as a variable in probit analysis to determine the IC50 value of the extract. There were 4 and 24 constituents of compounds detected using GC-MS in the ethanol and ethyl acetate extracts of the plant leaves respectively. Antiplasmodial assay of the extracts showed that ethyl acetate solvent gave stronger suppression against Plasmodium falciparum with an IC50 value of 21,29 µg/mL compared to ethanol solvent with an IC50 value of 21,29 µg/mL In conclusion plant leaf extract of Sansevieria trifasciata is potential to be developed as antimalarial ingredient.

Detection of (CTX-M) resistance genes in Extended Spectrum Beta-lactamases bacterial isolates of Escherichia coli and Klebsiella pneumonia and the antibacterial effect of ethanolic extract of Neem plant (Azadirachta indica) against these bacteria.

Detection of (CTX-M) resistance genes in Extended Spectrum Beta-lactamases bacterial isolates of Escherichia coli and Klebsiella pneumonia and the antibacterial effect of ethanolic extract of Neem plant (Azadirachta indica) against these bacteria.

Determination of the antifungal activity of Dorema ammonium extract

The current investigation included estimating the efficiency of aqueous and ethanolic plant extracts of Dorema ammoniacum plant in inhibiting the growth of fungi, where the ethanolic extract recorded a higher rate of inhibition than the aqueous extract. It is more effective when the concentration is increased in inhibiting the growth of two types of Candida, C. albicans and C. krusei. The aqueous extracts of Dorema ammoniacum had no toxic effect on human red blood cells at concentrations (100, 200, 300) mg/ml at an incubation period of 3 hours. As a result of the widespread use of medicinal plants as antimicrobial drugs, the absence of toxicity on the host cells is considered essential because it is an inexpensive, easy-to-apply, quick-results method and is safe when used as a drug. Keywords: Dorema ammoniacum, D. Don, cytotoxicity activity, Antimicrobial activity.

Anti-cancer effects of the extracts of broad and spirale cultivars of Codiaeum variegatum (L.) Blume on MCF-7, HepG2, and HeLa cell lines

Introduction: Codiaeum variegatum (L.) Blume is a well-known ornamental foliage plant used as a vegetable in northern Thailand, and it is the source of numerous bioactive substances. This work explored the effects of leaf extracts of broad (BCE) and spirale (SCE) cultivars of C. variegatum on three cancer cells, including human breast, human liver, and human cervical cancer cells. Methods: Ethanolic plant extracts were prepared, and then, 2,2-diphenyl-1-picryhydrazyl (DPPH), ferrous iron chelating, and lipid peroxidation assays were used to examine the flavonoid and phenolic compounds. The proliferative inhibition, growth, and migration of MCF-7, HepG2, and HeLa cancer cells, as a result of exposure to the extracts, were investigated. The extracts were investigated for their anti-cancer activities using sulforhodamine B (SRB), clonogenic, and wound-healing methods. Results: The data demonstrated that BCE and SCE contained high phenolic compounds. However, both extracts showed inactive anti-oxidant activities. Both extracts had high cytotoxicity on three types of cancer cells in a dose- and time-dependent manner after 24-72 hours of incubation with IC50 values in a range of 208-830 µg/mL. Moreover, the prepared extracts of C. variegatum significantly inhibited colony-forming ability and cell migration on all types of cancer cells. Compared with BCE, SCE showed more potent anti-cancer activities. Conclusion: These findings revealed that SCE had higher anti-cancer activities on MCF-7, HepG2, and HeLa cancer cells than BCE. Consequently, the SCE might be used as an effective chemotherapeutic compound for the prevention and treatment of cancer.

Croton Oil-induced Hemorrhoidal Rats: Evaluation of Anti-inflammatory Mediated Anti-hemorrhoidal Activity of Tridax procumbens Whole Plant

Background: Hemorrhoids are anorectal disorders characterized by dilation of rectal blood vessels, inflammation, and prolapse of the anal tissue. The disease affects both men and women equally. In consequence, the increasing prevalence of the disease needs potential agents with efficiency and low cost to support the treatment. Objective: The treatment of hemorrhoids in modern medicine is still found to be in its infancy, as there is no precise treatment for the specific disease. Tridax procumbens Linn (T. procumbens) belongs to the family Asteraceae. From the literature, the plant was found to have a traditional use for the treatment of hemorrhoids. Hence, the present research was focused on evaluating the antiinflammatory mediated anti-hemorrhoidal potential of ethanolic whole plant extract of T. procumbens (EWETP) in croton oil-induced hemorrhoidal rats. Methods: An in vitro study was conducted to evaluate the percentage inhibition of protein (egg albumin) denaturation by Tridax procumbens. This study determined the initial protective role of plant extracts against inflammatory proteins. An in vivo, anti-inflammatory-mediated anti-hemorrhoidal study was carried out on T. procumbens in various groups of croton oil-induced hemorrhoidal rats. Wistar albino rats were selected for the present research, and hemorrhoids were induced in experimental animals using a croton oil preparation containing 6% croton oil, deionised water, pyridine, and diethyl ether. Pilex ointment was taken as a reference drug in this study. Inflammation seems to be a major pathway for the progression of hemorrhoids. Hence, in the present research, Evans blue (EB) extravasation technique was applied to quantify inflammatory proteins. On the last day of the study, blood samples were drawn from experimental animals to analyse serum and blood for TNF- α, IL-6, and the percentage of neutrophils count. Recto anal coefficient was calculated to measure croton oil-induced rectal inflammation in animals. Histopathological studies were carried out separately on a second set of animals to identify the protective role of T.procumbens on rectal tissue cell histology. Molecular docking studies were carried out to rule out the possible interaction of plant phytoconstituents with the COX-2 enzyme. Results: Results showed that intra rectal application of croton oil preparation in albino rats developed hemorrhoids by elevating serum TNF-α and IL-6 in positive control group animals compared to normal group rats. Treatment of albino rats with T. procumbens at doses of 200 mg/kg and 400 mg/kg in groups IV and V has shown a significant reduction in serum TNF-α and IL-6. Furthermore, in the study, T. procumbens exhibited a significant dose-dependent reduction of EB dye extravasation in study animals. The study observations also revealed the inhibitory effect of plant extract on the blood percentage of neutrophils count and recto anal coefficient compared to the positive control group rats. Histopathological studies conducted on rectoanal tissues showed the presence of minimal rectal tissue changes in plant extract-treated group animals compared to positive control group rats. Molecular docking studies explored the possible interaction of phenolic compounds of plant extract with the COX enzyme. Conclusion: It was concluded that Tridax procumbens had a protective role against inflammatory mediators in hemorrhoids. In hemorrhoidal rats, ethanolic leaf extract was found to reduce the plasma percentage of neutrophils and other inflammatory cytokines, TNF-α, and IL-6. It could be used as a therapeutic anti-inflammatory mediated anti-hemorrhoidal agent.

Acute Toxicity of Ethanol Extract and A Fraction Of Sansevieria Trifasciata Prain Using The Brine Shrimp Lethality Test (BSLT)

Sansevieria trifasciata Prain plants are simple to grow in Indonesia and have therapeutic potential. The potential toxicity effect present in medicinal plants is important to be identified for safety assurance. The objective of this study is to identify the potential acute toxicity of leaves ethanol extract and fraction of snake plants (S.trifasciata). This study used the Brine Shrimp Lethality Test (BSLT) method. S. trifasciata leaves are extracted with ethanol by maceration. The ethanol extract was then fractionated using n-hexane, ethyl acetate, and n-butanol solvents. Extract and fraction S. trifasciata leaves with concentrations of 1000, 500, 100, 50, and 10 µg/ml were exposed to Artemia salina (L.). larvae for 24 hours. The level of toxicity is determined based on LC50 obtained based on the number of dead larvae, through probit analysis. The ethanol extract and the fraction of S.trifasciata leaves are active cytotoxic characterized by LC50 of 251,187 ppm for the ethanol extract, 116,950 ppm for the n-hexane fraction, 76,033 ppm for the ethyl acetate fraction, 20,230 ppm for the n-butanol fraction and 34,751 for the remaining fraction. The ethanol extract, n-hexane fraction, ethyl acetate fraction, and the remaining fraction were categorized as toxic, while the n-butanol fraction was categorized as very toxic.

Pharmacological evaluation of whole plant ethanol extract of <i>Thesium viride</i> against <i>Plasmodium berghei</i> and <i>Trypanosoma brucei</i> inoculated into BALB/c mice

Malaria and African trypanosomiasis are major public health issues in Africa, and urgent efforts are needed to identify new anti-protozoal drugs with high effectiveness and low toxicity. This study was designed to determine the pharmacological activities of whole plant ethanol extract of Thesium viride on a Plasmodium berghei and Trypanosoma brucei in BALB/c mice model. The whole dried plant sample was collected from Zaria Local Government Area in Kaduna State, and the plant was processed according to standard methods. Forty (40) adult BALB/c mice (Mus musculus) were randomly allocated to two major groups, A and B, to determine the anti-plasmodial and anti-trypanosomal activities of the plant extract, respectively. In group A, each mouse in subgroups I to V was inoculated with 0.1 mL containing 106 P. berghei merozoites. In group B, each mouse in subgroups I to V was inoculated with 0.1 mL containing 106 T. brucei trypomastigotes. Following patency of 3 days, all mice in groups A and B were either non-treated, treated with standard drug or treated at varying dosages of the ethanol extract for four days. Parasitaemia level, packed cell volume, rectal temperature, and body weight of the experimental mice were measured. Results generated indicate established that whole plant ethanol extract of Thesium viride showed a significant (p< 0.01) suppressive ability against Plasmodium berghei in all the mice administered 200, 400 and 800 mg/kg/day, compared to Trypanosoma brucei in mice administered similar dosages. Additionally, the extract showed better anti-weight loss ability in Plasmodium berghei-infected mice compared to Trypanosoma brucei-infected mice. The study concludes that the ethanol extract of Thesium viride has anti-protozoal prospects and should be explored for further pharmacological investigation.

Antimalarial and Antioxidant Activities of Ethanolic Stem Bark Extract of Terminalia macroptera in Swiss Albino Mice Infected with Plasmodium berghei.

Reduction of oxidative stress during malaria infection is considered as being of great benefit so long as treatment and drug development approaches are concerned. This study had the aim of evaluating the antimalarial and antioxidant activities of the ethanolic extract of Terminalia macroptera in Swiss albino mice infected with the Plasmodium berghei NK65 strain. In vivo, the antiplasmodial activity of the plant ethanolic extract was tested in a four-day suppressive and curative assay using P. berghei in Swiss albino mice. The extract was administered to the mice at doses of 125, 250, and 500 mg/kg per day. Then, parameters, such as parasite suppression and survival time of the mice, were evaluated. Furthermore, the effect of plant extract on liver damage, oxidative stress indicators, and lipid profile changes in P. berghei-infected mice were studied. Administration of T. macroptera significantly suppressed P. berghei infection by 55.17%, 70.69%, and 71.10% at doses of 125, 250, and 500 mg/kg, respectively, whereas chloroquine had 84.64% suppression relative to the untreated group 1% Dimethyl sulfoxide (1% DMSO) at day 4 (post-infection) in the four-day suppressive test. This suppression activity rate was dose-dependent. The curative test also presented a significant reduction in parasitemia and an extension of the survival time of the treated groups. Treatment of infected parasitized mice with the extract of T. macroptera had a significant (p < 0.05) reduction in parameters, such as total protein, aspartate aminotransferase, and alanine aminotransferase. Infection may also lead to a significant increase in the enzymatic activity of liver catalase and superoxide dismutase compared with the normal control group. The non-enzymatic antioxidant activity in parasitized mice was significantly reduced in malondialdehyde and increased in glutathione and nitric oxide when compared with the normal control group. These findings support the ethnobotanical use of T. macroptera stem bark as an antimalarial remedy coupled with antioxidant activity. However, further in vivo toxicity tests are required to ascertain its safety.