Ethanol Extract Of Aerial Parts Research Articles

Antimicrobial activity, acute toxicity and cytoprotective effect of Crassocephalum vitellinum (Benth.) S. Moore extract in a rat ethanol-HCl gastric ulcer model.

BackgroundA decoction of Crassocephallum vitellinum (Benth.) S. Moore (Asteraceae) is used in Kagera Region to treat peptic ulcers. This study seeks to evaluate an aqueous ethanol extract of aerial parts of the plant for safety and efficacy.MethodsAn 80% ethanolic extract of C. vitellinum at doses of 100, 200, 400 and 800 mg/kg body wt was evaluated for ability to protect Sprague Dawley rats from acidified ethanol gastric ulceration in comparison with 40 mg/kg body wt pantoprazole. The extract and its dichloromethane, ethyl acetate, and aqueous fractions were also evaluated for acute toxicity in mice, brine shrimp toxicity, and antibacterial activity against four Gram negative bacteria; Escherichia coli (ATCC 25922), Salmonella typhi (NCTC 8385), Vibrio cholera (clinical isolate), and Streptococcus faecalis (clinical isolate). The groups of phytochemicals present in the extract were also determined.ResultsThe ethanolic extract of C. vitellinum dose-dependently protected rat gastric mucosa against ethanol/HCl insult to a maximum of 88.3% at 800 mg/kg body wt, affording the same level of protection as by 40 mg/kg body wt pantoprazole. The extract also exhibited weak antibacterial activity against S. typhi and E. coli, while its ethyl acetate, dichloromethane and aqueous fractions showed weak activity against K. pneumonia, S.typhi, E. coli and V. cholera. The extract was non-toxic to mice up to 5000 mg/kg body wt, and the total extract (LC50 = 37.49 μg/ml) and the aqueous (LC50 = 87.92 μg/ml), ethyl acetate (LC50 = 119.45 μg/ml) and dichloromethane fractions (88.79 μg/ml) showed low toxicity against brine shrimps. Phytochemical screening showed that the extract contains tannins, saponins, flavonoids, and terpenoids.ConclusionThe results support the claims by traditional healers that a decoction of C.vitellinum has antiulcer activity. The mechanism of cytoprotection is yet to be determined but the phenolic compounds present in the extract may contribute to its protective actions. However, the dose conferring gastro-protection in the rat is too big to be translated to clinical application; thus bioassay guided fractionation to identify active compound/s or fractions is needed, and use of more peptic ulcer models to determine the mechanism for the protective action.

Antibacterial and Antioxidant Activities of Ursolic Acid and Derivatives

Ursolic acid, an important bioactive compound, was isolated from ethanol extract of aerial parts of Sambucus australis. In order to develop bioactive ursolic acid derivatives, two semi-synthetic compounds were obtained through modification at C-3. The antibacterial activity of the ursolic acid and its derivatives was investigated. The microdilution method was used for determination of the minimal inhibitory concentration (MIC), against twelve bacterial strains. The influence of ursolic acid and its derivatives on the susceptibility of some bacterial pathogens to the aminoglycosides antibiotics neomycin, amikacin, kanamycin and gentamicin was evaluated. The most representative synergistic effect was observed by 3β-formyloxy-urs-12-en-28-oic acid at the concentration of 64 μg/mL in combination with kanamycin against Escherichia coli (27), a multidrug-resistant clinical isolate from sputum, with reduction of MIC value from 128 μg/mL to 8 μg/mL. Ursolic acid and its derivatives were examined for their radical scavenger activity using the DPPH assay, and showed significant activity.

Studies on Chemical Constituents of Caesalpinia bonduc L. Roxb

A new compound caesanol 1 and a known diterpene 6β, 7β-dibenzoyloxyvouacapen-5α-ol 2 is isolated for the first time from ethanolic extract of aerial part of this plant. Structures of both were elucidated on the basis of spectroscopic analysis.

Antioxidant Property of Aerial Parts and Root ofPhyllanthus fraternusWebster, an Important Medicinal Plant

In present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC50 of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis of Fe3+-Fe2+ transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus, P. fraternus extract can be used as potent natural antioxidant.

Radical scavenging and antimicrobial activity of essential oil and extracts of Echinophora sibthorpiana Guss. from Macedonia

This study was undertaken to determine the antioxidant and antimicrobial effect of essential oil and extracts of Echinophora sibthorpiana Guss. (fam. Apiaceae) collected in Macedonia. The chemical composition of E. sibthorpiana essential oil was characterized by the presence of methyl eugenol (60.40%), p-cymene (11.18%) and ?-phellandrene (10.23%). The free radical scavenging activity of extracts and essential oil was evaluated by DPPH and ABTS assays. The aqueous extract of aerial parts exhibited the strongest scavenging activity (IC50=1.67 mg/ml); results of the ABTS test showed that the most effective was the ethanol extract of aerial parts (1.11 mg vit. C/g). The essential oil showed stronger antioxidant activity compared to hydroxyanisole, ascorbic acid and quercetin that were used in the DPPH and ABTS tests, respectively. The total phenolic and flavonoid concentrations in the extracts ranged between 38.65-60.72 mg GA/g, and 3.15-19.00 mg Qu/g, respectively. The antimicrobial properties of the extracts and essential oil were investigated using a micro-well dilution technique against human pathogenic strains. The results were comparable with the effects of the positive controls, streptomycin and fluconazole. These findings indicate that E. sibthorpiana extracts and oil can be used in preventive treatments and as an alternative for synthetic preservatives.

Anti-inflammatory and antioxidant activities of ethanolic extract of aerial parts of Vernonia patula (Dryand.) Merr.

ObjectiveTo investigate the inflammatory and antioxidant activities of ethanolic extract of aerial part of Vernonia patula (Dryand.) Merr (EAV). MethodsThe anti-inflammatory activity of EAV was studied using carrageenan and histamine-induced rat paw edema test at different doses (100, 200 and 400 mg/kg body weight). DPPH free radical scavenging, nitric oxide scavenging, reducing power and Fe2+ ion chelating ability were used for determining antioxidant activities. ResultsThe EAV, at the dose of 400 mg/kg, showed a significant anti-inflammatory activity (P<0.01) both in the carrageenan and histamine-induced oedema test models in rats, showing 62.86% and 64.42% reduction in the paw volume comparable to that produced by the standard drug indomethacin (67.26% and 66.01%) at 5 h respectively. In DPPH free radical scavenging test, IC50 value for EAV was found fairly significant 36.59 μg/mL when compared to the IC50 value of the reference standards ascorbic acid 8.97 μg/mL. The IC50 values of the extract and ascorbic acid were 47.72 and 12.39 μg/mL, respectively in nitric oxide scavenging assay. The IC50 value of the EAV (33.59 μg/mL) as percentage of Fe2+ ion chelating ability was also found significant compared to that of EDTA (9.16 μg/mL). The maximum absorbance for reducing power assay was found to be 1.928 at 100 μg/mL when compared to 2.449 for standard ascorbic acid. The total phenolic content was 198.81 mg/g of gallic acid equivalent. Acute toxicity test showed that the plant might be safe for pharmacological uses up to a dose level of 3 200 mg/kg of body weight in rats. ConclusionsTherefore, the obtained results suggest the acute anti-inflammatory and antioxidant activities of the EAV and thus provide the scientific basis for the traditional uses of this plant part as a remedy for inflammations.

Preliminary phytochemical and preclinical toxicity studies of Grewia serrulata DC

BackgroundThe present study was aimed to evaluate phytochemical constituents and the safety of aqueous and ethanolic extracts of aerial parts of Grewia serrulata DC (AEGS & EEGS) by determining their potential toxicity after acute and 28-day repeated dose administration in Wistar Albino rats. MethodsThe phytochemical analysis was done by standard laboratory grade reagents. Acute and 28-day repeated dose oral toxicity studies were performed by the following OECD test guide lines 423 and 407 respectively. ResultsThe present study reveals the presence of complex phytochemical constituents like flavanoids, saponins, glycosides, terpenoids, steroids and phenols. In acute toxicity study no treatment related death or toxic signs were observed with AEGS and EEGS administration. In repeated dose study no significant differences in body weight changes and hemotological paremeters were observed between control and AEGS and EEGS groups. Conversely there was a decrease in serum glucose and cholesterol levels and an increase in protein levels in treated rats compared to control. No gross pathological findings and difference in relative organ weights were observed between control and treated rats. Histopathological examination revealed no abnormalities with the test drug treatment. ConclusionPreliminary phytochemical evaluation shows the presence of various bioactive constituents. Acute toxicity study reveals that LD50 of AEGS and EEGS is greater than 2000 mg/kg body weight (b wt) in fasted female rats and can be classified under category 5. The 28-day repeated oral toxicity study justified that the No Observed Adverse Effect Level (NOAEL) of G. serrulata DC (GS) is greater than 800 mg/kg b wt/day P.O in rats. There were no delayed effects in GS satellite group. In conclusion GS was found to be non-toxic in tested doses and experimental conditions.

Antioxidant property of Grewia serrulata DC and its hypoglycemic effect on normal and hyperglycemic rats

AimThe present study was aimed to evaluate the antioxidant property and hypoglycemic activity of aqueous and ethanolic extracts of aerial parts of Grewia serrulata DC (AEGS & EEGS) in normal and hyperglycemic male Wistar Albino rats. MethodsThe antioxidant property was evaluated both in vitro and in vivo model using standard procedures. The hypoglycemic activity was evaluated by normal glucose and oral glucose tolerance test (NG-OGTT) method and by acute administration of AEGS and EEGS extracts at doses 200 and 400 mg/kg body wt p.o in Streptozotocin (STZ) induced hyperglycemic rats. ResultsThe ethanolic extract of Grewia serrulata DC exhibited strong free radical scavenging activity as evidenced by their low IC50 values in DPPH (1,1-diphenyl-2-picryl-hydrazyl) (9.16 ± 1.05 μg/ml), Superoxide (35.59 ± 1.6805 μg/ml) and nitric oxide (151.80 ± 1.79 μg/ml) methods. In in vivo study, treatment with ethanolic extract at both doses shows a significant dose dependent increase (p < 0.05 to p < 0.001) in the levels of superoxide dismutase (SOD) and Catalase (CAT) and a significant decrease (p < 0.05 to p < 0.001) in the levels of Thiobarbituric acid reactive substances (TBARS), when compared to CCl4 treated control in both liver and kidney. In NG-OGTT method EEGS shows reduction in glucose levels in normal rats and glucose loaded hyperglycemic rats and in STZ-induced hyperglycemic rats. ConclusionEEGS was found to possess strong antioxidant activity and good hypoglycemic activity compared to that of AEGS.

Two new steroidal glycosides isolated from the aerial part of Solanum torvum Swartz

One novel C-22 steroidal lactone saponin, namely solanolactoside C (1), and one new spirostanol glycoside, namely torvoside Q (2), were isolated from the ethanolic extract of aerial parts of Solanum torvum Swartz. The structures of 1 and 2 were determined by extensive NMR experiments including 1H NMR, 13C NMR, DEPT, 1H–1H COSY, HSQC, HMBC and ROESY and HRESIMS to be solanolide 6-O-β-d-quinovopyranoside and neochlorogenin 6-O-[β-d-xylopyranosyl-(1 → 3)-β-d-glucopyranoside], respectively.

Thymus capitatus from Tunisian arid zone: Chemical composition and in vitro anthelmintic effects on Haemonchus contortus

The increasing prevalence of anthelmintic resistant strains of helminths, the drug residues in animal products and the high cost of conventional anthelmintics has created an interest in studying medicinal plants as an alternative source of anthelmintics. Thymus capitatus (Lamiales: Lamiaceae) is used traditionally by people as spices and reported to possess some biological effects. The objective of this study is to evaluate the anthelmintic efficacy of T. capitatus in comparison to albendazole against the gastrointestinal nematodes of sheep. To fulfil the objectives, in vitro anthelmintic activities of crude aqueous and crude ethanolic extracts of aerial parts of T. capitatus were investigated on the eggs and adults of the nematode parasite Haemonchus contortus. Both extract types of T. capitatus completely inhibited egg hatching at a concentration close to 2mg/ml. LC50 of ethanolic extract of T. capitatus was 0.368mg/ml while that of aqueous extract was 6.344mg/ml (p<0.05). The ethanolic extract showed higher in vitro activity against adult parasites than the aqueous one in terms of the paralysis and/or death of the worms at different hours post-treatment. Dose dependent effect was observed for both extracts. Chemical analyses revealed that the overall profile of both extracts was dominated by oxygenated constituents. In addition, ethanolic extract is mainly composed of phenols among which thymol (71.22%) and camphor (17.18%).As far as the literature could be ascertained, this is the first publication on anthelmintic activity of T. capitatus. The results of the present study suggest that T. capitatus extracts are a promising alternative to the commercially available anthelmintics like albendazole for the treatment of small ruminants’ gastrointestinal nematodes.

Antioxidant Abilities, Phenolics and Flavonoids Contents in the Ethanolic Extracts of the Stems and Leaves of Different Stevia rebaudiana Bert Lines

We carried out a systematic research on antioxidant activities of ethanolic extracts of aerial parts (stems and leaves) of four selected Stevia rebaudiana lines. Total phenolics and flavonoids contents of different S. rebaudiana lines were in the range of 55.64–58.35 mg (gallic acid) g−1 dw and 48.29–60.33 mg (rutin) g−1 dw, respectively. The contents of phenolics and flavonoids, and antioxidant capacities in the leaves of S. rebaudiana were twofold to fivefold significantly higher than that in stems of S. rebaudiana. SR-3 had the highest total phenolics and flavonoids contents and the strongest scavenging abilities to DPPH and ABTS radicals. DPPH and TEAC assays were equal to evaluate the antioxidant ability of ethanolic extract of S. rebaudiana. The obtained results clearly indicated that the leaves of S. rebaudiana had an important value of utilization and development as a natural antioxidant agent and further studies for SR-3 should be put into practice.

Evaluation of antinociceptive and antipyretic effect of Pupalia lappacea Juss

Pupalia lappacea Juss (Family: Amaranthaceae) is claimed to be useful in treatment of bone fracture, wounds, boils, cough, toothache, fever and malaria. The study was aimed to evaluate the 80% aqueous ethanolic extract of aerial parts of P. lappacea for antinociceptive and antipyretic activities to verify the traditional claim. The extract was orally administered at doses of 200, 400 and 600 mg/kg. The extract has significantly (P&lt;0.01) reduced the nociception induced by acetic acid. The effect produced was in dose dependent manner. The antinociceptive effect was not reversed by pretreatment with naloxone in acetic acid induced writhing test. In hot plate method, the extract has significantly increased the latency time of jump. The naloxone has partially antagonised the antinociception of extract in hot plate test indicating P. lappacea has morphinomimetic properties. In the study of the CNS-depressant effects, the extract was found to produce significant reduction in head pokes and locomotion in mice by using hole board and locomotor activity test respectively. The extract has significantly reduced the rectal temperature in yeast induced pyrexia in rats at 600 mg/kg. The activity produced by extract was in dose dependent manner. Phytochemical investigation of ethanolic extract of P. lappacea revealed the presence of steroids and/or triterpenoids, flavonoids and phenolic compounds which may be responsible for antinociceptive and antipyretic activity of P. lappacea.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13193 International Current Pharmaceutical Journal 2013, 2(2): 23-28

Screening of plants used in Danish folk medicine to treat depression and anxiety for affinity to the serotonin transporter and inhibition of MAO-A

Ethnopharmacological relevanceA number of plant species are used in Danish folk medicine for treatment of depression and anxiety. Materials and methodsAqueous and ethanolic extracts of 17 plant species were tested for affinity to the serotonin transporter and for inhibition of MAO-A—both targets for antidepressive treatment. ResultsAn ethanolic extract of aerial parts of Borago officinalis had affinity to the serotonin transporter. Ten extracts, from eight plants, had IC50 values below 25μg/ml extract in the MAO-A assay. The most active extracts in the MAO-A assay were the ethanol extract of seeds of Trigonella foenum-graecum (IC50 4μg/ml); ethanol extract of leaves of Apium graveolens (IC50 5μg/ml) and the water extract of aerial parts of Calluna vulgaris (IC50 8μg/ml). ConclusionsBesides Borago officinalis, which toxicity profile excludes it from further development as an herbal drug, none of the plants had potential as serotonin reuptake inhibitors. Several plants had MAO-A inhibitory activity.

Phytochemical screening and evaluation of cardioprotective activity of ethanolic extract of Ocimum basilicum L. (basil) against isoproterenol induced myocardial infarction in rats.

Background and the purpose of the studyThe objectives of the present study were phytochemical screening and study of the effects of ethanolic extract of aerial parts of Ocimum basilicum (basil) on cardiac functions and histopathological changes in isoproterenol-induced myocardial infarction (MI).MethodsThe leaves of the plant were extracted with ethanol by maceration and subjected to colorimetry to determine flavonoids and phenolic compounds. High-performance TLC analysis and subsequent CAMAG's TLC scanning were performed to quantify rosmarinic acid content. Wistar rats were assigned to 6 groups of normal control, sham, isoproterenol, and treatment with 10, 20, and 40 mg/kg of the extract two times per day concurrent with MI induction. A subcutaneous injection of isoproterenol (100 mg/kg/day) for 2 consecutive days was used to induce MI.ResultsPhytochemical screening indicated the presence of phenolic compounds (5.36%) and flavonoids (1.86%). Rosmarinic acid was the principal phenolic compound with a 15.74% existence. The ST-segment elevation induced by isoproterenol was significantly suppressed by all doses of the extract. A severe myocardial necrosis and fibrosis with a sharp reduction in left ventricular contractility and a marked increase in left ventricular end-diastolic pressure were seen in the isoproterenol group, all of which were significantly improved by the extract treatment. In addition to in-vitro antioxidant activity, the extract significantly suppressed the elevation of malondialdehyde levels both in the serum and the myocardium.ConclusionThe results of the study demonstrate that Ocimum basilicum strongly protected the myocardium against isoproterenol-induced infarction and suggest that the cardioprotective effects could be related to antioxidative activities.

Anticancer activity of Pupalia lappacea on chronic myeloid leukemia K562 cells

BackgroundCancer is one of the most prominent human diseases which has enthused scientific and commercial interest in the discovery of newer anticancer agents from natural sources. Here we demonstrated the anticancer activity of ethanolic extract of aerial parts of Pupalia lappacea (L) Juss (Amaranthaceae) (EAPL) on Chronic Myeloid Leukemia K562 cells.MethodsAntiproliferative activity of EAPL was determined by MTT assay using carvacrol as a positive control. Induction of apoptosis was studied by annexin V, mitochondrial membrane potential, caspase activation and cell cycle analysis using flow cytometer and modulation in protein levels of p53, PCNA, Bax and Bcl2 ratio, cytochrome c and cleavage of PARP were studied by Western blot analysis. The standardization of the extract was performed through reverse phase-HPLC using Rutin as biomarker.ResultsThe results showed dose dependent decrease in growth of K562 cells with an IC50 of 40 ± 0.01 μg/ml by EAPL. Induction of apoptosis by EAPL was dose dependent with the activation of p53, inhibition of PCNA, decrease in Bcl2/Bax ratio, decrease in the mitochondrial membrane potential resulting in release of cytochrome c, activation of multicaspase and cleavage of PARP. Further HPLC standardization of EAPL showed presence 0.024% of Rutin.ConclusionPresent study significantly demonstrates anticancer activity of EAPL on Chronic Myeloid Leukemia (K562) cells which can lead to potential therapeutic agent in treating cancer. Rutin, a known anti cancer compound is being reported and quantified for the first time from EAPL.

Anxiolytic and Hypnotic Effects of Aqueous and Ethanolic Extracts of Aerial Parts of Echium italicum L. in Mice

Research in the area of herbal psychopharmacology has clearly improved in recent decades. Self-administration of herbal medicines has been the most popular therapeutic alternative to standard medicine. Since the extract of Echium amoenum exhibits an anxiolytic effect, the aim of this study is to evaluate the anxiolytic and hypnotic effects in mice of the aqueous and ethanolic extracts of aerial parts of E. italicum, a member of the Boraginaceae family. Mice were administered the agents intraperitoneally before the start of the experiments for evaluation of hypnotic activity (induced by sodium pentobarbital, 30 mg/kg, i.p.), anxiolytic activity (elevated plus-maze [EPM] test), locomotor activity (open field test), and motor coordination (rotarod test). The ethanolic and aqueous extracts of E. italicum, at doses of 1.2 and 2.1 g/kg, increased the percentage of time-spent and the percentage of arm entries in the open arms of the EPM and decreased the percentage of time-spent in the closed arms of the EPM. Moreover, both extracts decreased the pentobarbital-induced latency to sleep and significantly increased the total sleeping time induced by pentobarbital. In addition, locomotor activity was affected by aqueous extracts and ethanolic extract (at higher doses). Both extracts showed no effect in the rotarod test. These results suggest that both ethanolic and aqueous extracts of E. italicum may have anxiolytic effects and sedative activity but no effect on muscle relaxation.

Anxiolytic and Hypnotic Effects of Aqueous and Ethanolic Extracts of Aerial Parts of Echium italicum L. in Mice

Anxiolytic and Hypnotic Effects of Aqueous and Ethanolic Extracts of Aerial Parts of Echium italicum L. in Mice

Evaluation of hepatoprotective activity of aqueous and ethanolic extract of Oxalis corniculata against intoxication of thioacetamide induced rats

The present study was carried out to evaluate the hepatoprotective activity of aqueous and ethanolic leaves extracts of Oxalis corniculata L., Oxalidaceae, against thioacetamide-induced hepatotoxicity. Hepatotoxicity was induced in Wistar rats of either sex by subcutaneous injection of thioacetamide. An aqueous and ethanolic extract of aerial parts of O. corniculata (200 and 400 mg/kg/day) were evaluated. Oral administration of O. corniculata aqueous and ethanolic leaves extract at 400 mg/kg resulted in a significant reduction in SGOT (146.42±2.54 and 136.75±1.37 IU/L respectively), SGPT (81.96±3.15 and 72.05±2.33 IU/L respectively), GGTP (16.6±0.49 and 15.02±0.68 IU/L respectively), ALP (241.86±3.94 and 202.42±5.37 IU/L respectively) and total bilirubin (0.226±0.00 mg/dL 0.288±0.01 mg/dL respectively) content that were lesser than positive control, thioacetamide damaged rats. Histology of the liver sections of the animals treated with the extract also showed dose dependent reduction of necrosis. Hence the study concluded that O. corniculata has potential hepatoprotective activity.

Anthelmintic Activity of Hygrophila difformis Blume

This study evaluated the anthelmintic activity of the petroleum ether, benzene, chloroform, ethanol extracts of aerial parts of Hygrophila difformis (Family: Acanthaceae). The Pheretima posthuma were used for all the experimental protocol due to their anatomical and physiological resemblance with the intestinal roundworms of ruminants and human beings. The worms were treated with the extract solutions containing three different concentrations (10, 25 and 30 mg/ml), for each extracts supplemented with 1% gum acacia in normal saline at 37 ± 1°C. Albendazole at a dose of 10 mg/ml was used as reference. The effectiveness of the extracts was judged on the basis of the loss of spontaneous movement and/or death of the worms. Qualitative phytochemical analysis of different extracts of the plant was carried out following the standard procedure. All extracts at different dose levels effectively paralyzed and killed the worm in dose-dependent fashion (p &lt; 0.001). Maximum anthelmintic activity was found with benzene extract. Cardiac glycosides, tannins, steroids, flavonoids and saponins were found positive in phytochemical analysis. The present study established the anthelmintic activity of H. difformis and can be utilized for the ruminants.

Total Phenolic and Total Flavonoids Content of Aerial Parts of Artemisia abrotanum Linn. and A. pallens Wall

This paper illustrates total phenolic, total flavonoid content, phytochemicals present and physical properties of oil obtained from Artemisia abrotanum and Artemisia pallens (Asteraceae). Total phenolic and flavonoid content was established by folin-ciocalteu assay and aluminum chloride colorimetric assay respectively. Studies in our laboratory shows the presence of alkaloids, terpenoids/ sterols, tannins, phenols, flavonoids in A. abrotanum and terpenoids/ sterols, tannins, phenols, flavonoids in A. pallens in ethanolic extract. The phenolic content in ethanolic extract of aerial parts of A. abrotanum and A. pallens was found to be 9.35 ± 0.008 mgGAE/g and 6.88 f 0.052 mgRE/g respectively, standard curve (Y = 0.01143X - 0.02093 and rz = 0.9994). The flavonoids content in ethanolic extract of aerial parts of A. abrotanum and A. pallens was found to be 11.0 ± 0.025 mgRE/g and 11.55 ± 0.006 mgRE/g respectively, standard curve (Y = 0.0471X - 0.0056 and r2 = 0.9984). The results suggests both plant contains significant amount of polyphenols and flavonoids hence could be considered in the category of antioxidant, anticancer, antimicrobial and immunomodulatory and is suitable candidate for activity guided isolation which will help in find out the new chemical entity (NCEs) responsible for the traditional claims.