Total Phenolic and Total Flavonoids Content of Aerial Parts of Artemisia abrotanum Linn. and A. pallens Wall

Abstract

This paper illustrates total phenolic, total flavonoid content, phytochemicals present and physical properties of oil obtained from Artemisia abrotanum and Artemisia pallens (Asteraceae). Total phenolic and flavonoid content was established by folin-ciocalteu assay and aluminum chloride colorimetric assay respectively. Studies in our laboratory shows the presence of alkaloids, terpenoids/ sterols, tannins, phenols, flavonoids in A. abrotanum and terpenoids/ sterols, tannins, phenols, flavonoids in A. pallens in ethanolic extract. The phenolic content in ethanolic extract of aerial parts of A. abrotanum and A. pallens was found to be 9.35 ± 0.008 mgGAE/g and 6.88 f 0.052 mgRE/g respectively, standard curve (Y = 0.01143X - 0.02093 and rz = 0.9994). The flavonoids content in ethanolic extract of aerial parts of A. abrotanum and A. pallens was found to be 11.0 ± 0.025 mgRE/g and 11.55 ± 0.006 mgRE/g respectively, standard curve (Y = 0.0471X - 0.0056 and r2 = 0.9984). The results suggests both plant contains significant amount of polyphenols and flavonoids hence could be considered in the category of antioxidant, anticancer, antimicrobial and immunomodulatory and is suitable candidate for activity guided isolation which will help in find out the new chemical entity (NCEs) responsible for the traditional claims.