The study was carried out to investigate the effect of growing media prepared by mixing different ratios of poplar sawdust (PS) and lavender extraction wastes such as lavender straw (LS) and lavender flower wastes (LFW), on the cultivation cycle, yield and biological efficiency (BE%), nutritional profil and antioxidant capacity of Pleurotus eryngii var. Ferulae mushroom. Moreover, functional and organic compounds of the fruiting body were examined by FTIR analysis. The mushroom production was experimented with seven different growing media. A raised yield of 216.3 g/kg ±6.4 g/kg and BE% of 61.8 ± 1.8% were recorded in the growing medium where the PS, LS and LWF were used in the ratio of 5:3:2, whereas the maximum protein content (17.94 ± 0.04 g100 g-1), total phenolic content (5.09 ± 0.08 mg GAE g−1), ABTS (54.36 ± 2.39 μmol TE g−1) and CUPRAC (58.89 ± 0.91 μmol TE g−1) were observed in the growing medium prepared with mixture of PS and LS (the ratio of 7:3). Moreover, all the growing media added LS and LFW resulted in improved mushroom yield, the nutritional profile and antioxidant capacity of P. eryngii var. Ferulae when compared to the control medium. According to the results, LS and LFW can be used as efficient, cost-effective and promising substrates for the production of P eryngii var. Ferulae, a valuable medicinal and edible mushroom species.