Abstract
This study sought to evaluate the effects of Phaseolus multiflorus var. albus Bailey extract (PM extract) and Pleurotus eryngii var. ferulae extract (PF extract) on the inhibition of digestive enzymes and to confirm the anti-obesity effect of DKB-117 (a mixture of PM extract and PF extract) in digestive enzyme inhibition in a mouse model of obesity induced by a high-fat diet. In in vitro studies, PM extract and PF extract have increased dose-dependent inhibitory activity on α-amylase (Inhibitory concentration (IC50 value: 6.13 mg/mL)) and pancreatic lipase (IC50 value; 1.68 mg/mL), respectively. High-fat diet–induced obese mice were orally administered DKB-117 extracts at concentrations of 100, 200, and 300 mg/kg/day, while a positive control group was given orlistat (pancreatic lipase inhibitor) and Garcinia cambogia (inhibiting the enzymes needed to synthesize carbohydrates into fat) at concentrations of 40 and 200 mg/kg/day, respectively, for eight weeks. As a result, body weight, fat mass (total fat mass, abdominal fat, and subcutaneous fat) detected with microcomputed tomography, fat mass (abdominal fat and inguinal fat) after an autopsy, and liver triglyceride levels were decreased significantly in the DKB-117 (300 mg/kg/day) group compared to those in the HFD control group. Additionally, we obtained results indicating that the presence of carbohydrates was found more in the DKB-117-300 (300 mg/kg/day) group than in the HFD control group. These data clearly show that DKB-117 extracts are expected to have an anti-obesity effect through a complex mechanism that promotes carbohydrate release through the inhibition of carbohydrate-degrading enzymes while blocking lipid absorption through lipase inhibition.
Highlights
Obesity is a metabolic disorder characterized by an excess accumulation of fat in the body due to one’s energy intake exceeding one’s energy expenditure [1]
Pancreatic lipase inhibitory activity was determined by measuring the hydrolysis of p-nitrophenyl butyrate (PNPB) to p-nitrophenol at 405 nm with the use of an enzyme-linked immunosorbent assay (ELISA) reader (Infinite 200 Pro; Tecan Austria GmBH, Grödig, Austria)
Α-amylase, one of the digestive enzymes secreted from the pancreas and salivary glands, is involved in important biological processes such as the digestion of carbohydrates. α-Amylase inhibitors are well known to be effective in reducing postprandial hyperglycemia by slowing the digestion of carbohydrates and absorbing postprandial glucose
Summary
Obesity is a metabolic disorder characterized by an excess accumulation of fat in the body due to one’s energy intake exceeding one’s energy expenditure [1]. PF water extracts reduced body weight, white adipose tissue weight, and liver weight in a mouse model of obesity induced by a high-fat diet (HFD) while improving glucose tolerance [1,13,14]. This study was carried out to develop a dietary supplement ingredient that improves convenience of use and has good safety and obesity effects by respectively mixing natural plants having α-amylase inhibitory and pancreatic lipase inhibitory effects. The optimum mixing ratio of PF extract and PF extract was selected by an in vitro test, and we sought to determine the anti-obesity effect of DKB-117 in digestive enzyme inhibition in a mouse model of obesity induced by HFD
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