Serum Enzyme Activities Research Articles

Ethanol intoxicated renal oxidative stress mitigated by poly-herbal formulation-Trasinareg; in murine model

Oxidative stress has a critical role in the pathophysiology of several kidney diseases. Safe and symptomatic medication to prevent the kidney oxidative stress id highly recommended. In this study the aim and objective of this scientific study was to find out the probable protective effect of poly-herbal formulation (Trasina®) on serum and kidney antioxidant enzymes activities in ethanol intoxicated organ dysfunctions in mice model. Forty Swiss albino adult male mice were randomly divided into four groups; Group-1 served as control, Group-2 orally treated with ethanol (50% v/v), Group-3 pre-treated with herbal medicine (Trasina®) along with ethanol (50% v/v), and Group-4 only treated with poly-herbal formulation (Trasina®) without ethanol daily. Completion of six weeks treatment the animals were euthanized and kidneys were immediately removed and used fresh or kept frozen until analysis. Before sacrifice blood samples were taken to measure the antioxidant parameters i.e. super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione –S transferees (GST) from sera. Super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione–S transferees (GST) activities in serum and kidney were significantly reduced in the ethanol intoxicated mice than in the controls. Treatment with herbal medicine (Trasina®) upon ethanol intoxication significant elevated the all antioxidant activities serum and kidney. Results obtained from the present study clearly predict that treatment with poly-herbal formulation (Trasina®) might be a potent antioxidant that exerts beneficial effects on both super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione –S transferees (GST) activities in ethanol intoxicated mice and inhibit organ damage.

Titanyum dioksit nanopartiküllerinin Cyprinus carpio’da bazı serum parametreleri ile serum enzim aktiviteleri üzerine akut toksisitesi

Titanyum dioksit nanopartikülleri (TiO2 NP) elektronik malzemeler, paketleme, yiyecek endüstrisi ve kozmetik ürünler başta olmak üzere yaygın kullanıma sahip nanopartiküllerdir. Bu yaygın kullanım sonucu doğaya daha yüksek düzeylerde salınmaları, özellikle su organizmaları için tehlike oluşturmaktadır. Bu araştırmada TiO2 NP’nin 25 ve 50 mg/L derişimlerinin 24, 48 ve 96 saatlik sürelerle etkisinde C. carpio’da serum glikoz, total protein ve kolesterol düzeyleri ile serum AST, ALT ve ALP enzim aktivitelerinin belirlenmesi amaçlanmıştır.
 Belirlenen süre ve derişimlerde TiO2 NP etkisinde balıklarda mortalite gözlenmemiştir. TiO2 NP etkisinde serum glikoz düzeyi her iki derişimde de 48 ve 96 saatlik etki süresinde kontrole oranla önemli düzeyde artış gösterirken, serum total protein ve kolesterol düzeyinde tüm süre ve derişimlerde kontrole oranla herhangi bir değişiklik gözlenmemiştir. Serum AST, ALT ve ALP enzim aktivitesi ise her iki derişimde de 96 saatlik etki süresinde kontrole oranla önemli düzeyde artış göstermiştir.

Effect of Swiftlet’s (Collocalia Fuciphago) Nest Extract on the Malondialdehyde (MDA) and Superoxyde Dismutase (SOD) Activity on Hyperglycemic Rattus Norvegicius

Hyperglycemia that occurs in diabetic Mellitus leads to glycation reactions in protein molecules and oxidative stress resulting in damage to cells and organs. Swiftlet’s nest believed society could lower blood glucose. The objective of the study was to evaluate the effect of Swiftlet’s nest (Collocalia fuciphago) extract on glucose level, Malondialdehyde (MDA) levels, and Superoxide Dismutase (SOD) activity in blood serum. The study used Posttest-Only with Control Group Design, consisting of 1 control group (given aqua dest) and 3 treatment groups (dose 1; 10 and 100 mg/kg BW). Each group consisted of 6 Rattus norvegicius. Before being treated, Streptozotocin-induced rat at a dose of 68 mg/kg BW intraperitoneal. On the 7th day after induction, rats had elevated glucose ± 102 - 108 mg/dL. Then the rats were given water extract Swiftlet’s nest for 28 days orally. All data were analyzed by the Kruskal-Wallis test, followed by the Mann-Whitney test, with a 95% confidence level. The results of blood glucose levels in each group (K, P1, P2 and P3) were 111.0 vs 88.5 vs 86 vs 83 mg / dL (p = 0.035), MDA levels experienced an increase in the treatment group compared to controls namely 193.50 vs 193.83 vs. 198.50 nmol / mL, p = 0.001. While the SOD enzyme activity has increased, namely 0.0050 vs. 0.0075 vs. 0.0263%. In conclusion, Swiftlet’s nest water extract can reduce blood glucose levels and increase MDA levels and SOD enzyme activity in blood serum.

Use of Chou’s 5-steps Rule to Study the Effect of Cereal Dietary Protein on Liver and Coronary Heart Disease Prevention

Background: The liver is a vital organ in the human body involved in the metabolic processes. The liver can be damaged due to factors such as protein deficiency, viral infection, as well as consumption of alcohol, chemical contaminants, and adulterated food. High blood cholesterol, high blood pressure, diabetes, lack of exercise, poor diet, obesity and cigarette smoking are the major risk factors for stroke, heart attack and coronary heart disease (CHD). In medical science, several synthetic drugs have been discovered and used for the treatment of people suffering from liver injury and CHD, but these are not always effective and sometimes difficult to manage by medical therapies and also found to be accompanied by other side effects. Objective: The study aimed to critically review the recent research and studies of epidemiological and randomized controlled trials to find out the effective cereal protein as an alternative preventive food to reduce the risk of CHD and protect the liver from viral hepatic diseases, focusing on daily food intake, body weight, liver weight, serum enzyme activities and cholesterols. Methods: A few of the data from our experiment were used. A literature search was performed for a reliable source of published research articles, review papers, and epidemiological and randomized controlled trials on the effects of cereal protein on animals and human intervention on Google, Google scholar, Redcube, Endnote, Scopus, SpringerDirect.com, PubMed and Web of Science. Then, the data was organized, summarized and analyzed. Results: In medical science, serum enzyme activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and lipid peroxidation stress malondialdehyde (MDA) are commonly used as biochemical markers of the liver-damaging agents. Blood cholesterols (total cholesterol-TC, triglyceride-TG, low-density lipoprotein cholesterol-LDLC and highdensity lipoprotein cholesterol-HDLC) are used as the markers of heart diseases. The review shows that daily food intake and body weight data are not significantly differed among normal diet, casein (CAS) and cereals protein. The millet and wheat protein increase the liver weight, whereas the rice protein lowers the liver weight. The intake of cereal protein significantly reduces the activities of serum AST, ALT, LDH, MDA, TC, TG and LDLC, whereas it increases the HDLC. Conclusion: Experimental, review and randomized controlled trials (RCTs) data confirm that cereal protein appears to be beneficial in reducing the hepatic liver injury and CHD by maintaining body weight, liver weight, blood pressure, serum enzyme activities of AST, ALT and LDH, lipid peroxidation stress MDA and cholesterol concentrations both in plasma and liver.

Ameliorating Effect of Green Tea Aqueous Extract against Histo pathophysiological Changes Induced by Ciprofibrate in the liver of Male Albino Rats

Background: The liver is a specific target for drug toxicity because of its role in removal and metabolism of chemicals by converting drugs into another forms that can be readily removed from the body. It is known that the main function of the liver is the elimination of toxins that may enter the body, thus becoming vulnerable damaged during this mechanism, which can be revealed as bleeding, congestion, necrosis or other conditions of liver injury. Ciprofibrate belongs to widely used class of lipid-regulating agents, which stimulate hepatic cells and the hepatic cell becomes uncontrollably divided, causing liver growth. It causes liver cell proliferation in addition to other pleiotropic effects such as peroxisome proliferation and induction of certain peroxisomal and cytosolic enzymes in liver. Objective: The present study aimed to evaluate the potential beneficial effects of green tea aqueous extract administration against the biochemical and histological alterations induced in the liver by ciprofibrate in male rats. Materials and Methods: In the current study 3 groups of 6 male rats were used (Control group, 100mg\Kg body weight, and Cipro 100mg\Kg body weight with green tea). The rats have been treated daily orally by gavages for 21 days. On the last day of the experiment the animals were killed then blood samples and parts from the liver were collected. Liver function was examined for the serum Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), alkaline phosphates (ALP), enzyme activities, and serum total & direct bilirubin concentrations. The histopathological investigation was conducted for the liver tissues of all groups. Results: Treatment of male rats with 100 mg\Kg body weight of ciprofibrate caused a significant increase in serum ALT, AST, and ALP activities, total, and direct bilirubin concentration. Histologically, there were histological changes in central vein area and portal zones, revealed congestion in blood sinusoids, necrosis in hepatic cells, and damage in central vein lining epithelium. Co-administration of green tea aqueous extract with Ciprofibrate significantly improved the structural changes in the liver and the serum ALT, AST, and ALP activities, total, and direct bilirubin concentrations were significantly declined. Conclusion: It can be concluded that Ciprofibrate treatment induced elevation in liver function tests and severe histopathological changes and green tea aqueous extract was able to protect the liver against these effects in male rats. So, the patients should be advised to take green tea aqueous extract while they are treated by ciprofibrate.

Determination of hepatoprotective and antioxidant role of thyme (origanum onites L.) infusion against ethyl alcohol induced oxidative stress in rats

In this study, we aimed to determine the hepatoprotective and antioxidants role of thyme (O. onites) against ethyl alcohol (EtOH) induced oxidative stress in rats. The experiment conducted as control, 20% EtOH, 3% O. onites infusion and 3% O. onites + 20% EtOH groups. The antioxidant and hepatoprotective role of O. onites infusion was evaluated by histopathological chance and serum damage biomarkers of liver (LDB), antioxidant constituents (ACs) and malondialdehyde (MDA). According to results, Liver damage serum enzymes activities were significantly higher in 20% EtOH group compared to normal control (NC) whereas the parameters were significantly lower in the infusion supplemented groups compared to 20% EtOH group. Also, MDA content of 20% EtOH group increased significantly in all tissues compared to NC group but decreased as coparion with 20% EtOH. The results suggest that the infusion restored the most of the parameters towards the NC with fluctuations in the ACs.

An urchin-like helical polypeptide-asparaginase conjugate with mitigated immunogenicity

The use of asparaginase (ASNase), a first line drug for lymphoma treatment, is impaired by short circulation and notoriously high immunogenicity. Although PEGylation can prolong the circulating half-life of ASNase, however, it also induces anti-PEG antibodies that lead to accelerated blood clearance (ABC) and hypersensitivity reactions. Here, we create an urchin-like polypeptide-ASNase conjugate P(CB-EG3Glu)-ASNase, in which the surface of ASNase is sufficiently shielded by an array of zwitterionic helical polypeptides through the labeling of the ε-amine of lysine. The conjugate is fully characterized with size exclusion chromatography, SDS-PAGE, dynamic light scattering, and circular dichroism. In vitro, P(CB-EG3Glu)-ASNase retains full activity based on the enzymatic assay using the Nessler's reagent and cell viability assay. In vivo, examination of the enzyme activity in serum indicates that P(CB-EG3Glu)-ASNase prolongs the circulating half-life of ASNase for ~20 fold. Moreover, P(CB-EG3Glu)-ASNase significantly inhibits tumor growth in a xenografted mouse model using human NKYS cells. Importantly, P(CB-EG3Glu)-ASNase elicits almost no antidrug or antipolymer antibodies without inducing ABC effect, which is in sharp contrast with a similarly produced PEG-ASNase conjugate that develops both antidrug/antipolymer antibodies and profound ABC phenomenon. Our results demonstrate that urchin-like conjugates are outstanding candidates for reducing immunogenicity of therapeutic proteins, and P(CB-EG3Glu)-ASNase holds great promises for the treatment of various lymphoma diseases.

A Serum Metabolic Profiling Analysis During the Formation of Fatty Liver in Landes Geese via GC-TOF/MS.

During the process of fatty liver production by overfeeding, the levels of endogenous metabolites in the serum of geese would change dramatically. This study investigated the effects of overfeeding on serum metabolism of Landes geese and the underlying mechanisms using a metabolomics approach. Sixty Landes geese of the same age were randomly divided into the following three groups with 20 replicates in each group: D0 group (free from gavage); D7 group (overfeeding for 7 days); D25 group (overfeeding for 25 days). At the end of the experiment, 10 geese of similar weight from each group were selected for slaughter and sampling. The results showed that overfeeding significantly increased the body weight and the liver weight of geese. Serum enzymatic activities and serum lipid levels were significantly enhanced following overfeeding. Gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS) was employed to explore the serum metabolic patterns, and to identify potential contributors to the formation of fatty liver and the correlated metabolic pathways. Relative to overfeeding for 7 days, a large number of endogenous molecules in serum of geese overfed for 25 days were altered. Continuous elevated levels of pyruvic acid, alanine, proline and beta-glycerophosphoric acid and reduced lactic acid level were observed in the serum of overfed geese. Pathway exploration found that the most of significantly different metabolites were involved in amino acids, carbohydrate and lipid metabolism. The present study exhibited the efficient capability of Landes geese to produce fatty liver, identified potential biomarkers and disturbed metabolic pathways in liver steatosis. These findings might reveal the underlying mechanisms of fatty liver formation and provide some theoretical basis for the diagnosis and treatment of liver diseases.

Follow up long term preliminary studies on carcinogenic and toxic effects of ochratoxin A in rats and the putative protection of phenylalanine

Carcinogenic effects of ochratoxin A (OTA) on liver, kidneys, intestine, lung and eyes of Wistar rats exposed to 10 ppm or 5 ppm OTA in the diet and additionally supplemented or not with phenylalanine (PHE) were examined during 24-months experimental period. OTA was seen to provoke strong degenerative changes and slight pericapillary oedema in most internal organs, e.g. kidneys, liver, intestine, spleen and brain. Six of total nine neoplasms were identified as malignant and three as benign. Five of total six malignant neoplasms and two of total three benign neoplasms were seen in male rats. The pathological finding in rats after two weeks feeding with OTA-contaminated feed was dominated by degenerative changes in various internal organs, which were weaker in the group additionally supplemented with PHE. The protective effect of PHE was evident with respect to OTA-induced decrease of serum glucose and serum protein, but this protection was not singnificant with respect to serum enzymes activity. The number of neoplasms in PHE-supplemented group exposed to 10 ppm OTA was similar to that in the group exposed to twice lower feed levels of OTA alone, suggesting about a possible protective effect of PHE. The rats would not be able to serve as experimental model for humans with regard to OTA-induced tumorigenesis, because the target organ of OTA-toxicity in humans and pigs is mainly the kidney as opposed to the significant damages and carcinogenic effects seen in various organs in rats exposed to OTA.

Кортико-вегетативна регуляція активності амінотрансфераз у сироватці крові холостих свиноматок за умови дії технологічного подразника

The article presents the results of studying the influence of cortical and vegetative mechanisms of regulation on the transaminase enzymes activity in blood serum of dry sows under exposure to a technological stimulus. The experiments were carried out on sows of large white breed of 3 years old. The results of research have shown that the indicators of conditioned reflex activity affect the activity of reamination enzymes in blood serum both under physiological conditions and after the exposure to a technological stimulus. According to the correlation analysis, the relationship between the strength and balance of cortical processes with serum alanine aminotransferase activity (r = 0.48–0.65; Р≤0.05–0.01), as well as the strength of cortical processes with aspartate aminotransferase activity (r = 0.51; P≤0.05). The significant influence (η2x = 0.23–0.36; Р≤0.01) of the balance of cortical processes on the activity of alanine aminotransferase in blood serum was established. Instead, the tone of the autonomic nervous system had a significant influence on the activity of reamination enzymes only after the exposure to a technological stimulus. In particular, the significant degree of influence (η2x = 0.28; P≤0.05) of sympathicotonia on the activity of alanine aminotransferase in blood serum on the 7th day after exposure to a technological stimulus was established.

Evaluation of the Protective Effect of Ademetionine, Cytoflavin, and Dihydroquercetetine on Blood Enzymes Activity in Rats Treated with High Doses of Sodium Valproate.

We evaluated the protective effect of ademetionine, cytoflavin, and dihydroquercetin on activity of serum enzymes in rats treated with high doses of sodium valproate for 28 days. Ademetionine and cytoflavin produced the greatest protective effect, the effect of dihydroquercetin was less pronounced. In rats treated with ademetionine, AST activity decreased as soon as on day 7 and remained at this level until the end of the experiment; ALT, alkaline phosphatase, and γ-glutamyl transferase activities decreased on days 21 and 28 of the study. Cytoflavin produced similar effects, the effect of dihydroquercetin was observed on days 21 and 28 for AST, ALT, alkaline phosphatase and on day 28 for γ-glutamyl transferase. These results substantiate the use of hepatoprotective drugs in case of long-term treatment with anticonvulsants in patients with epilepsy.

Inflammatory responses, energy metabolism enzymes, oxidative status in Clostridium perfringens infection in broilers

The aim of this study was to determine whether infection by Clostridium perfringens negatively interferes with the oxidant/antioxidant status and activity of energy metabolism enzymes (creatine kinase (CK), adenylate kinase (AK) and pyruvate kinase (PK)), as well as the zootechnical performance of broilers. A completely randomized design with three treatments, with five replications by treatment, and 10 birds by repetitions was used: T1: non-infected group; T2: group infected with C. perfringens; T3: group infected with C. perfringens, and basal diet with performance enhancers (antibiotic and coccidiostatic). At 21 days of age, the birds were experimentally infected orally with 4.0 x 108 CFU/mL C. perfringens. At strategic moments during the experimental period, zootechnical data and blood samples were collected. There were no significant differences among treatments in terms of weight gain, feed intake, or feed conversion. At 20 days, heterophils counts were significantly lower in the T3 group birds than in the others. A significant reduction in the number of total leukocytes, because of the lower number of lymphocytes, was observed in groups T2 and T3 on day 34. Neutrophil and monocyte counts were significantly lower in group T3 than in group T1 on day 34. On day 42, there were significantly lower levels of total protein and globulin in birds in T3 than in T1. On day 27, PK activity was significantly higher in groups T2 and T3; similar behavior observed on day 34 when CK and PK activities in T2 and T3 were significantly higher than those of T1. AK activity was significantly higher in the serum of T3 birds than in those of the other groups at 34 days. At 41 days, CK and PK activities were significantly higher only in birds in group T3 compared to other groups. The levels of reactive oxygen species and lipoperoxidation, as well as the activity of glutathione S-transferase, were significantly lower in birds from group T3 compared to other groups at age 20 days. After a challenge with C. perfringens, there were fluctuations in the behavior of oxidative stress biomarkers; in particular, at 41 days of age, birds in T2 had elevated levels of lipoperoxidation, different from what was seen in birds in T3 that consumed feed with a performance enhancer. Preliminary results suggest that clostridiosis affects serum activity of enzymes in the phosphotransfer network, requiring compensatory enzymatic changes in an attempt to maintain energy homeostasis. In addition, the infection reduces the number of inflammatory cells and causes oxidant/antioxidant imbalance that may contribute to the pathophysiology of the disease. Además, la infección reduce el número de células inflamatorias y provoca un desequilibrio en el estado oxidante / antioxidante que puede contribuir a la fisiopatología de la enfermedad.

Optimizing the immunization procedure of single-walled carbon nanotubes based vaccine against grass carp reovirus for grass carp

Hemorrhagic disease caused by grass carp reovirus (GCRV) is considered to be one of the most serious threats to grass carp, resulting in significant economic loss in grass carp culture industry. This study was aimed at optimizing the immunization program of SWCNTs-vaccine (SWCNTs-M-VP4-3) controlling infectious GCRV, determining the best immunization program for grass carp, and then providing reference for the commercial promotion and use of vaccines in aquaculture. We designed an compete experiment to optimize different parameters affecting vaccination such as immunization time, antigen concentration, and fish density when grass carp was vaccinated by bath treatment. Our results showed that the highest relative percent survival (88.33%) was found in 12 h of immunization time, 10 mg L−1 of antigen concentration, and 15 fish per liter of fish density compared to 100% mortality for control groups. And other immune responses (serum antibody production, enzyme activities, and immune genes expression) also demonstrated similar results. These results indicated that the administration protocol which induce highest immune response of the host, had the highest vaccine effect against the disease. This study lays the foundation for providing reference for the commercial promotion and use of vaccines in aquaculture.

Suppression of inflammatory cascades via novel cinnamic acid nanoparticles in acute hepatitis rat model

Hepatitis was characterized by extreme inflammation and hepatocellular damage. Therefore, the current study aimed to gain insights into the modulation role of Cinnamic acid nanoparticles (CANPs) against acute hepatitis induced by d-Galactosamine and gamma radiation exposure (D-Gal/radiation) in the rat model and to suggest the implied molecular mechanism of CANPs. Acute hepatitis seriousness and the serum enzyme activities of ALT, AST, and ALP have been diminished upon oral administration of CANPs. Besides, the hepatic tissue levels of malondialdehyde (MDA) and nitric oxide (NO) have been significantly decreased, and the total antioxidant activity (TAO) depletion was extremely restored. Furthermore, the reduction of hepatic damage caused by pretreatment with CANPs was accompanied by significant suppression in the levels of hepatic proinflammatory cytokines (TNF-α, IL-1β, and IL-18), NF-κB, NLRP3, caspase-1 and proapoptotic protein BAX whereas anti-apoptotic protein Bcl-2 level significantly elevated as compared with D-Gal/radiation-induced acute hepatitis (AH) group. Also, CANPs suppress the D-Gal/radiation-induced IL-1β, IL-18, and ASK1 mRNA gene expression and the protein expression of TLR4 and MyD88 in the hepatic tissue. These biochemical parameters are confirmed by histological examination of the liver tissues. The present results indicated that CANPs can protect the hepatic cells from damage by both its anti-inflammatory and antioxidant influence as well as by modulating oxidation cellular pathways that have contributed to the acute severity of hepatitis. Also, CANPs is capable of suppressing apoptosis. Consequently, Nanoparticles of Cinnamic acid have the medicinal ability to protect the liver from acute hepatitis.

Protective immunity of largemouth bass immunized with immersed DNA vaccine against largemouth bass ulcerative syndrome virus

To reduce the largemouth bass ulcer syndrome (LBUSV) aquatic economic losses, it must take effective preventive measures and coping strategies should be urgently investigated. In this research, the effects of a functionalized single-walled carbon nanotubes (SWCNTs) applied as a delivery vehicle for DNA vaccine administration in largemouth bass (Micropterus Salmoides) against LBUSV were studied. Our results showed that SWCNTs loaded with DNA vaccine induced a better protection to largemouth bass against LBUSV. We found more than 10 times increase in serum antibody levels, enzyme activities and immune-related genes (IL-6, IL-8, IFN-γ, IgM and TNF-α) expression, in the SWCNTs-pcDNA-MCP immunized groups compared with PBS group and the pure SWCNTs group. The survival rates for control group (PBS), pure SWCNTs groups (40 mg L−1), four pcDNA-MCP groups (5 mg L−1, 10 mg L−1, 20 mg L−1 and 40 mg L−1) and four SWCNTs-pcDNA-MCP groups (5 mg L−1, 10 mg L−1, 20 mg L−1 and 40 mg L−1) were 0%, 0%, 0%, 2.77%, 11.11%, 19.44%, 27.78%, 38.89%, 52.78% and 61.11%, respectively. Our results demonstrate that the SWCNTs-DNA vaccine can be used as a new method against LBUSV showing protection following challenge with LBUSV.

Effect of Moringa oleifera Lam. Leaf Extract Against Aluminium Chloride Induced Hippocampal Histology and Serum Enzyme activities in adult Wistar rats

Introduction: Aluminium, a ubiquitous metal implicated in some neurodegenerative diseases is linked to activation of free oxygen species. The antioxidant-rich plants, Moringa oleifera (MO) is reported to protect against Aluminium activities. This study investigated the actions of MO leaf extract (MOLE) against Aluminium chloride (AlCl3 )- induced hippocampal cellular changes and serum levels of alkaline phosphatase (ALP), aspartate transaminase (AST) and alanine transaminase (ALT) in adult Wistar rats. Materials and Methods: Thirty Wistar rats weighing between 150 g and 220 g were grouped (n=5) into; 1-control (5 mL/kg distilled water), 2-AlCl3 (100 mg/kg), 3-low dose MOLE (250 mg/kg), 4-high dose MOLE (1,000 mg/kg), 5-concurrent AlCl3 and low dose MOLE, and 6-concurrent AlCl3 and high dose MOLE. All administrations were by oral gavages for 21 days. On day 22, following deep anaesthesia and cardiac puncture, blood was obtained for serum enzyme analysis, and the brain perfusion fixed, harvested and processed for histological study. Results: Results showed significantly (p < 0.05) higher ALP level in the AlCl3 group compared with the control, as well as the other test groups. However, there was no significant (p > 0.05) AST and ALT levels. The hippocampal CA3 of the AlCl3 group showed hypertrophic cells, with some of the cells having karyorrhectic features. The concurrent AlCl3 and low and high doses, MOLE groups showed less of these adverse features. Conclusion: These results suggest that MOLE may protect enzymatic activities against Aluminium chloride. However, its action on hippocampus is still subject to further investigation.

The cross-talk of NLRP3 inflammasome activation and necroptotic hepatocyte death in acetaminophen-induced mice acute liver injury.

Overdose acetaminophen (APAP) can result in severe liver injury, which is responsible for nearly half of drug-induced liver injury in western countries. Previous studies have found that there existed massive hepatocellular necrosis and severe inflammatory response in APAP-induced liver injury. However, the mechanistic linkage between necroptosis and NLRP3 inflammasome pathway in APAP-induced hepatotoxicity remains poorly understood. In order to investigate the relationship between inflammation and hepatocytes death in APAP hepatotoxicity, a time-course model for APAP hepatotoxicity in C57/BL6 mice was established by intraperitoneal (i.p) injection of 300 mg/kg APAP in this study. The activity of serum enzymes and pathological changes of APAP-treated mice were evaluated, and the critical molecules in necroptosis and NF-κB-NLRP3 inflammasome signaling pathway were determined by immunoblot and immunofluorescence analysis. The results demonstrated that APAP overdose resulted in a severe liver injury. Furthermore, the expression of critical molecules in NLRP3 inflammasome and necroptosis pathways peaked at 12-24 h, and then was decreased gradually, which is consistent with the pattern of pathological injury induced by APAP. Our further investigation found that the level of IL-1β in mouse liver was closely correlated with the level of phosphorylated MLKL following exposure to APAP. Furthermore, inhibition of necroptosis with necrostatin-1 significantly suppressed the activation of NLRP3 inflammasome signaling. Taken together, our results highlighted that the cross-talk between necroptosis and NLRP3 inflammasome played a critical role for promoting APAP-induced liver injury. Inhibition of the interaction of inflammation and necroptosis by pharmaceutical methods may represent a promising therapeutic strategy for APAP-induced liver injury.

Diagnostic and economic efficiency of schemes for differential diagnosis of chronic hepatitis and liver cirrhosis, based on the determination of interleukin-13, alpha-1-defensin and enzymatic activities in serum

The search for non-invasive criteria for the differential diagnosis of chronic hepatitis and liver cirrhosis is one of the urgent tasks of hepatology. Purpose: to evaluate the diagnostic and economic efficiency of differential diagnostic schemes for the determination of chronic hepatitis and liver cirrhosis of alcoholic and unspecified etiology, based on the determination of interleukin-13, alpha-1-defensin and enzymatic activities in blood serum. Material and method: The study included 41 patients with chronic hepatitis, 102 patients with liver cirrhosis of alcoholic and unspecified etiology. The control group included 43 donors from the blood transfusion station. The levels of enzymatic activities, interleukin-13 and alpha-1-defensin of blood serum were determined. Results: Hyaluronidase and elastase activities, as well as serum interleukin-13 and alpha-1-defensin, showed high diagnostic efficiency in detecting chronic diffuse inflammatory liver diseases, and trypsin-like and deoxyribonuclease activities in differential diagnosis of chronic hepatitis and liver cirrhosis. The developed differential diagnostic schemes allow differentiating chronic hepatitis and liver cirrhosis with a high odds ratio and have a high economic effect in comparison with Fibrotest and liver biopsy. The inclusion of interleukin-13 and alpha-1-defensin in the examination scheme allows to increase the diagnostic efficiency. Conclusions: Schemes for the differential diagnosis of chronic hepatitis and liver cirrhosis based on enzymatic activity, interleukin-13 and alpha-1-defensin of blood serum allow to improve the results of diagnostic measures, and also have a high economic effect in comparison with Fibrotest and liver biopsy.

Effect of glutamine on the blood biochemistry and the activity of serum enzymes in broilers challenged with Salmonella enteritidis

This study was conducted to investigate the effects of glutamine (Gln) on blood biochemistry and activity of serum enzymes in broilers challenged with Salmonella enteritidis. 240 one-day-old Arbor Acres broilers were distributed into four groups treated in a completely randomized design for 21 days. The groups included a noninfection control (CON); infected with S. enteritidis (SCC); infected +0.5% Gln (Gln 1), and infected +1.0% Gln (Gln 2). The plasma total protein, albumin, and globulin concentration, AST, LDH and CK activity in SCC group were lower than the CON overall except the albumin at 14, 21 d, and CK at 14 d. SCC group had lower RBC at 7 d; lower MCHC at 14 d; higher MCHC, and MCH at 7 d, or 21 d; higher WBC at d 7, 14, and 21; higher γ-GGT activity at d 14, and 21than the CON group. Gln group had higher plasma total protein, albumin, and globulin concentration, AST, LDH and CK activity than those in SCC overall except the albumin at 14, 21 d, and CK at 14 d. Gln group had higher RBC at 7 d; higher MCHC at 14 d; lower MCHC, and MCH at 7 d, or 21 d; lower WBC at d 7, 14, and 21, and lower γ-GGT activity at d 14, and 21 than the SCC group. These results suggest that Gln could effective improve the physiological responses and maintain the normal health status of S. enteritidis infection of broilers.

Foliar application of zinc and selenium alleviates cadmium and lead toxicity of water spinach – Bioavailability/cytotoxicity study with human cell lines

The present study investigated the effects of foliar application of zinc (Zn) and selenium (Se) on bioavailability of Zn and Se and toxicity of cadmium (Cd) and lead (Pb) to different water spinach ecotypes (LA and HA) grown in slightly (XZ) or moderately (LJY) contaminated fields via in vitro digestion combined with Caco-2/HL-7702 cell model. The obtained results revealed that foliar application of Zn and Se promoted yield, increased total, bioaccessible and bioavailable fractions of Zn and Se in plants, indicating that foliar application is a feasible way of biofortification. Although there was no significant effect on liver cell proliferation (MTT), membrane stability (LDH) and hepatocyte enzyme (ALT and AST) activities, the obvious ecotype and soil dependent fluctuations of lipid peroxidation (MDA) and antioxidant enzyme (SOD, POD and CAT) activities in serum highly suggest that the low accumulator and clean field should be used in agricultural production rather than the high accumulator and contaminated farmland. Moreover, foliar application of Zn and Se improved nutritional quality of all water spinach genotypes in both fields, including increased Fe, vitamin C, cellulose and chlorophyll, maintained concentrations of potassium (K), manganese (Mn), copper (Cu), protein, and nitrate. These results demonstrate that this agricultural management practice may prove to be an effective approach for minimizing health risk and alleviating “hidden hunger” in the developing countries.