Enhanced Nitrogen Removal Research Articles

Synergistic electro-thermal enhancement of nitrogen removal in an iron-based Anammox system at low temperatures

In this study, a novel AC-stimulation technology was explored by synergistic electro-thermal enhancement for nitrogen removal in a Fe-based Anammox at low temperatures. Highest total nitrogen (TN) removal efficiency achieved by the AC-stimulated Anammox system was 73.59 % at 0.45 mA/cm2 AC densities, compared to 45.72 % TN removal efficiency realized in the control (non-AC-stimulated) Anammox system, both conducted at 13 °C. Additionally, the Anammox enzymes HZS and HDH exhibited the highest copy numbers at the same current density. Results from response surface methodology (RSM) analysis indicated that the optimal conditions included electrode spacings of 3.89 cm, 4.11 cm, and 3.78 cm, and AC densities of 0.43 mA/cm2, 0.46 mA/cm2, and 0.39 mA/cm2 at temperatures of 13 °C, 16 °C, and 19 °C, respectively. Mechanism analysis revealed that the electrons provided by AC could stimulate biological processes while simultaneously heating the material.

Aerobic granular sludge enhances start-up and granulation in single-stage partial nitritation anammox granular sludge systems: Performance, mechanism, and shifts in bacterial communities

The rapid start-up and granulation of a single-stage partial nitritation anammox granular sludge (PN/AnGS) system under limited seed sludge conditions is crucial for its practical application. This study proposed an aerobic granular sludge (AGS) − based strategy, enhanced the enrichment of anammox bacteria (AnAOB), and shortened the start-up time of PN/AnGS system by 20.5%. In addition, the inoculation of AGS can ensure the stable operation of the system during the selective sludge discharge to washout the flocs. Microbial community structure, particle size distribution, morphology results showed that niche shift was the key to promote the enrichment of AnAOB and AGS played a decisive role in the particle characteristics of PN/AnGS. Since AGS can be directly obtained from full-scale AGS wastewater treatment plants, integrating PN/AnGS with AGS processes can transition wastewater treatment from a “linear economy” to a “circular economy”, enhancing nitrogen removal efficiency and delivering significant economic and environmental benefits.

Enhanced anammox-mediated nitrogen removal in bioelectrochemical systems at prolonged negative electrode potentials.

Bioelectrochemical anaerobic ammonium oxidation (anammox) systems allow eco-friendly removal of nitrogen from reject wastewater coming from biogas processing as the anammox bacteria have previously shown to have c-type cytochromes acting in the extracellular electron transport (EET) mechanism between the bacteria and electrode. The anammoxosome compartment present in anammox bacteria features a highly curved membrane and contains tubular structures along with electron-dense particles that contain iron, which could enhance the process of EET and enhance nitrogen removal by properly applied potentials. In this study, nitrogen removal was investigated in the electrostimulated anammox nitrogen removal (EANR) cells operated comparatively at open circuit and at applied potentials of - 300mV, - 500mV, and - 700mV vs. Ag/AgCl. At peak performance (at - 700mV vs. Ag/AgCl), the EANR showed up to 140% higher specific nitrogen removal rate (11.2 ± 0.3g N/m2/day) compared to the control reactors without applied potential (8.3 ± 0.2g N/m2/day). The microbial community on the cathode with the applied potential had a higher relative proportion of unclassified Candidatus Brocadia (7.5%) compared to inoculum (> 0.01%), in contrast to cathode without potential (0.74%) and control (0.2%). The EANR system demonstrated to achieve ammonium and nitrite removal efficiencies of 91% and 53%, respectively, during a 24-h test cycle from an initial TN concentration of ~ 100mg N/L. After 150h, it achieved complete removal of all nitrogen compounds, reaching a 100% removal efficiency. The EANR would be very useful in the establishment of field-scale bilateral anammox-bioelectrochemical technology combining microbial fuel cell bioanodes and EANR biocathodes for wastewater treatment.

A Review of Enhanced Nitrogen Removal Measures and Mechanisms in Constructed Wetlands

A Review of Enhanced Nitrogen Removal Measures and Mechanisms in Constructed Wetlands

Enhanced nitrogen removal from low strength anaerobic membrane bioreactor (AnMBR) permeate using complete nitrification and partial denitrification-anammox processes

Enhanced nitrogen removal from low strength anaerobic membrane bioreactor (AnMBR) permeate using complete nitrification and partial denitrification-anammox processes

Enhanced nitrogen removal from secondary effluent of municipal wastewater using denitrification filter: Feasibility of refractory organics as a carbon source

Conventional advanced nitrogen removal in municipal wastewater is hindered by the limited availability of carbon sources in the secondary effluent. However, refractory organics present in it had the potential to serve as intrinsic carbon sources after hydrolysis for nitrogen removal via simultaneous denitrification and partial-denitrification anammox (PDA) processes. To assess this potential, a denitrification filter was set up in this study to evaluate its feasibility of concurrent processes. Results showed that increasing influent ammonium (NH4+-N) from 1.0 to 7.0 mg/L increased total nitrogen (TN) removal from 52.4 % to 89.9 %. Simultaneous occurrence of PDA and denitrification process were confirmed by the actual chemical oxygen demand (COD) consumption (0.8–1.2 mg/mg TN removal) from non-fluorescent organics. The presence of the anammox, hydrolytic and denitrifying bacteria further supported the achievement of nitrogen removal through PDA and denitrification processes by utilizing hydrolytic products biodegraded from refractory organics.

Efficient nitrogen and phosphorus removal performance and microbial community in a pilot-scale anaerobic/anoxic/oxic (AOA) system with long sludge retention time: Significant roles of endogenous carbon source

Stringent wastewater discharge standards require wastewater treatment plants (WWTPs) to focus on enhancing nitrogen (N) and phosphorus (P) removal efficiency. Increasing sludge concentration by regulation of sludge retention time (SRT) would enhance wastewater treatment loads. However, phosphorus-accumulating organisms (PAOs) would be outcompeted by glycogen-accumulating organisms (GAOs) under long SRT, leading to a collapse of P removal. In this work, pilot-scale anaerobic-oxic-anoxic (AOA) and anaerobic-anoxic-oxic (AAO) systems with long SRT (30 d) were parallelly established for actual urban wastewater treatment. The results indicated that sludge reflux ratio, temperature, and C/N ratio significantly impact N and P removal performance of AOA and AAO systems with long SRT, and removal efficiency of AOA system significantly exceeded that of AAO system. AOA system with long SRT achieved the optimal performance at sludge reflux ratio of 200%, temperature of 25 °C, and C/N ratio of 8, with COD, NH4+-N, TN, and PO43--P removal ratio of 92.80 ± 2.24%, 97.38 ± 0.89%, 88.97 ± 2.47%, and 94.33 ± 3.27%, respectively. In addition, compared to AAO system, AOA system could save 23.08% of the aeration volume. This work highlighted that AOA system with long SRT included multiple coupled nitrogen and phosphorus removal pathways, such as autotrophic/heterotrophic nitrification, anoxic/oxic denitrification, endogenous denitrification, and denitrifying phosphorus removal. Among these, the synergistic effect of endogenous denitrification and denitrifying phosphorus removal driven by internal carbon sources contributed to satisfactory nitrogen and phosphorus removal efficiency in AOA system with long SRT.

Integrating Suspended Sludge and Fixed Film into a Biological Wastewater Treatment System to Enhance Nitrogen Removal

Integrated fixed-film activated sludge (IFAS) technology greatly enhances nitrogen removal effectiveness and treatment capacity in municipal wastewater treatment plants, addressing the issue of limited land availability. Hence, this method is appropriate for treating household wastewater from office buildings. The research was conducted at the wastewater treatment plant in an office building in Ho Chi Minh City, Vietnam. Experiments were conducted to ascertain the most favorable working conditions, including hydraulic retention time (HRT), alkalinity dosage, and dissolved oxygen (DO). According to the study, the IFAS system had the highest nitrogen removal effectiveness when operated at a hydraulic retention time (HRT) of 7 h, an alkalinity dose of 7.14 mgCaCO3/mgN-NH4+, and a dissolved oxygen (DO) value of 6 mg/L. The nitrification efficiency ranges from 89.2% to 98.8%. The N-NO3− concentration post-treatment is within the range of 27–45 mgN-NO3−/L, which is lower than the allowable discharge limit of 60 mg/L as per Vietnam’s wastewater discharge requirements. The research findings have enhanced the efficiency of the office building management process, thereby promoting the sustainable growth of society.

Enhancing nitrogen removal from digested swine wastewater by anammox with aeration optimization coupling real-time control strategy

The nitrogen removal of anaerobically digested swine wastewater (ADSW) through partial nitritation and anammox is hindered by the challenge of balancing aeration between ammonia oxidizing bacteria (AOB) and anammox bacteria (AnAOB). This study focused on optimizing aeration through a real-time control strategy in an integrated fixed-film activated sludge reactor for treating ADSW. The system implemented a dual aeration mode that included both low dissolved oxygen (DO) (< 0.4 mg/L) and short-term high DO (0.6–1.2 mg/L), with pH, oxidation–reduction potential, and NH4+-N electrode values as real-time control parameters. NH4+-N removal rate increased from 3.37 to 12.82 mgN/(gVSS·h), and total nitrogen (TN) removal rate enhanced from 0.14 to 0.25 kgN/(m3·d). Increasing DO stimulated AOB activity by 31 % and provided sufficient NO2−-N for AnAOB. The r-strategist AnAOB (Candidatus Kuenenia) proliferated well in the biofilm (0.25 % in flocs vs. 1.86 % in biofilm). The enrichment of denitrifiers improved organic matter and TN removal.

Enhancing nitrogen removal in low C/N wastewater with recycled sludge-derived biochar: A sustainable solution

Denitrification is an important biological process in wastewater treatment plants (WWTPs). However, a low carbon-to-nitrogen (C/N) ratio limits the availability of organic carbon, potentially reducing denitrification efficiency. This study investigates the impact of sludge-derived biochar on the nitrogen removal of activated sludge for low C/N ratio municipal wastewater. Sludge-based biochar was characterized by its physicochemical properties, revealing that biochar prepared at 400 °C exhibited the highest specific surface area and the most favorable surface functional groups for electron transfer. The results from batch tests showed that adding 4 g/L of biochar dosage enhanced denitrification rates and total nitrogen (TN) removal efficiency the most. Sequencing batch reactors (SBRs) experiments further confirmed that biochar dosgae improved the removal efficiencies of COD, NH4+-N, and TN, achieving stable values of 97.2 ± 1.2 %, 99.2 ± 0.6 %, and 83.8 ± 2.4 %, respectively. Metabolic and electrochemical analyses revealed that biochar addition enhanced the activity of denitrification enzymes, increasing the ammonia oxidation rate by 12.9 ± 0.7 %, nitrite oxidation rate by 14.7 ± 1.2 %, nitrate reduction rate by 36.9 ± 1.5 %, and nitrite reduction rate by 16.4 ± 0.8 %. The relative abundance of denitrification functional genes (amoA, nirS, nirK, narG, nosZ) increased, and the activities of the corresponding enzymes (AMO, NXR, NAP, NIR) rose by 23±6 %, 53±5 %, 260±15 %, and 55±7 %, respectively. This increase in enzyme activity suggested enhanced denitrification processes, which was further supported by the 60.1 ± 3.7 % increase in electron transfer system activity (ETSA), indicating that biochar acted as an electron shuttle. This study proposes a potential sustainable approach for sludge recycling and enhanced wastewater nitrogen removal under low C/N conditions.

Enhanced nitrogen removal and microbial community of the mainstream deammonification treating fluctuating influent C/N wastewater by the novel functional carriers

The plug-flow fixed bed reactors with zeolite/tourmaline-modified polyurethane carriers (PFBRZTP) and polyurethane carriers (PFBRPU) were operated to assess the fluctuating influent C/N impact on the system performance and the carrier effect on the enhancing the system operation. Result suggested that fluctuations in influent C/N and variations in operational temperature reduced the removal performance and system stability within PFBRPU. The negative impact of C/N fluctuation could be effectively mitigated by effluent reflux. In contrast, PFBRZTP performance and operational stability of maintained at high level with a greater nitrogen removal rate (0.18 kg N·(m³·d)-1). Redundancy analyses showed that the fluctuations in influent C/N dramatically affected the microbiome structure in PFBRPU, and the leading influencing factor was shifted to the fluctuating amount of influent C/N, which in turn reduced the system performance and stability. ZTP carriers could maintain the balance of main functional bacterial activity and abundance and promote the partial denitrification process with a higher Thauera abundance of 0.48%.

Enhancing nitrogen removal by simultaneous nitritation and denitritation in a multi-cycle SBR with supplementation of solid carbon sources

Simultaneous nitritation and denitritation have the potential to significantly improve nitrogen removal in sewage treatment processes. However, their application in low-strength sewage treatment systems presents challenges. This study explored the impact of four solid carbon sources (SCSs) on N-removal via nitrite in a multi-cycle SBR with biocarriers. Results showed that both N-removal efficiencies and nitrite accumulation rates increased with higher COD/N ratios, indicating that high COD/N ratios can improve the competition between denitrifiers and nitrite-oxidizing bacteria for nitrite, leading to stable simultaneous nitritation and denitritation. The supplementation of SCSs further enhanced this high-efficiency N-removal process, with polybutylene succinate (PBS) and polycaprolactone (PCL) showing greater increases in N-removal via nitrite than poly-hydroxybutyrate (PHB) and poly-hydroxyalkanoate (PHA). Moreover, PBS showed the most significant increase in denitrification efficiency in anoxic conditions, while PHA was the most effective external SCS at a moderate level of dissolved oxygen. These findings suggest that the incorporation of external SCSs can facilitate the simultaneous nitritation and denitrification process in multi-cycle SBRs, underscoring the importance of selecting an appropriate SCS for optimizing nitrogen removal in sewage treatment projects.

Optimized ammonium-nitrate ratios in constructed wetlands for simultaneously efficient nitrogen removal and greenhouse gas reduction

Constructed wetlands (CWs) are increasingly employed for treating secondary tailwater treatment from wastewater treatment plants (WWTPs), addressing the challenges of nitrogen removal and greenhouse gas (GHG) emission reduction. This study explores the potential for enhancing nitrogen removal while reducing GHG emissions in vertical flow CWs by optimizing the influent ammonium-nitrate ratio in real tailwater scenarios. Six different influent ammonium-nitrate ratios (0:15, 1:20, 1:10, 1:5, 1:2, 1:1) were investigated and the results indicate that an ammonium-nitrate ratio of 1:5 achieves the highest total nitrogen (TN) removal efficiency of 77.50 ± 2.40 %, with the effluent concentrations below 4 mg/L. Conversely, a ratio of 1:10 minimized N2O emissions (0.027 mg/(m2⋅h)), representing just 0.054 ‰ of influent TN. Additionally, it was observed that increasing the ammonium-nitrate ratio influences microbial selectivity within the system. Moreover, a life cycle assessment further indicates that a 1:5 ratio poses the lowest potential environmental impact. These insights guide the design optimization of CWs for enhanced nitrogen removal and GHG mitigation, providing valuable strategies for tailwater management from WWTPs.

Enhanced nitrogen removal in constructed wetlands with multivalent manganese oxides: Mechanisms underlying ammonium oxidation processes

The ammonium (NH4+) removal efficiency in constructed wetlands (CWs) is often limited by insufficient oxygen. In this study, an extract of Eucalyptus robusta Smith leaves was used to prepare multivalent manganese oxides (MVMOs) as substrates, which were used to drive manganese oxide (MnOx) reduction coupled to anaerobic NH4+ oxidation (Mnammox). To investigate the effects and mechanisms of MVMOs on ammonium nitrogen (NH4+-N) removal, four laboratory-scale CWs (0 %/5 %/15 %/25 % volume ratios of MVMOs) were set up and operated as continuous systems. The results showed that compared to controlled C-CW (0 % MVMOs), Mn25-CW (25 % MVMOs) improved the average NH4+-N removal efficiency from 24.31 % to 80.51 %. Furthermore, N2O emissions were reduced by 81.12 % for Mn25-CW. Isotopic tracer incubations provided direct evidence of Mnammox occurrence in Mn-CWs, contributing to 18.05–43.64 % of NH4+-N removal, primarily through the N2-producing pathway (73.54–90.37 %). Notably, batch experiments indicated that Mn(III) played a predominant role in Mnammox. Finally, microbial analysis revealed the highest abundance of the nitrifying bacteria Nitrospira and Mn-cycling bacteria Pseudomonas, Geobacter, Anaeromyxobacter, Geothrix and Novosphingobium in Mn25-CW, corresponding to its superior NH4+-N removal efficiency. The enhancement of NH4+ oxidation, first to hydroxylamine and then to nitrite, in Mn25-CW was attributed to the upregulation of ammonia monooxygenase genes (amoABC and hao). This study enhanced our understanding of Mnammox and provided further support for the use of manganese oxide substrates in CWs for efficient NH4+-N removal.

Autotrophic Sulfur-Driven Partial Denitrification as a Sustainable Nitrite Supply Pathway for Anammox: Insights on Enhanced Nitrogen Removal and Microbial Synergies

Autotrophic Sulfur-Driven Partial Denitrification as a Sustainable Nitrite Supply Pathway for Anammox: Insights on Enhanced Nitrogen Removal and Microbial Synergies

Application of Biofloc-Down flow hanging sponge system to remove nitrogen components in recirculating zero water exchange aquaculture system

This study presents a novel approach to sustainable aquaculture by integrating biofloc technology (BFT) with a compact down-flow hanging sponge (DHS) reactor. The integrated BFT-DHS system effectively removed nitrogen compounds while maintaining ammonia–nitrogen (NH4+-N) concentrations below 1 mg-N L-1 without water exchange. Application of this system in a tank bred with juvenile Oreochromis niloticus showed a high NH4+-N removal rate of up to 97 % and nitrite (NO2– −N) concentrations were maintained at 0.1 ± 0.1 mg-N L-1. Microbial analysis revealed Gordonia as the predominant genus in the biofloc contributing to heterotrophic nitrification, while the Peptostreptococcaceae family dominated the DHS reactor. Heterotrophic nitrification seemed to be the primary process for enhanced nitrogen removal. Pathogenic bacteria, Vibrio sp. was absent throughout the study. This study highlights the potential integration of BFT and DHS system for sustainable aquaculture practice with effective nitrogen removal.

Robust nitrogen removal via nitrification-partial denitrification / anammox to co-treat acrylic fiber wastewater and sewage without external carbon

Acrylic fiber (AF) wastewater is characterized by high levels of ammonia nitrogen, refractory organic compounds, and toxic substances, making biological nutrient removal challenging. Conventional biological treatment technologies are often ineffective due to the complex composition, high toxicity, and low C/N ratios of AF wastewater. In this study, a two-stage nitrification-partial denitrification/anammox (N-PDA) process was firstly developed for the combined treatment of AF wastewater and domestic sewage, which integrated nitrification-SBR(N-SBR) and partial denitrification/anammox-UASB (PDA-UASB). The N-SBR achieved stable nitrification (ARE=100 %, HRT=4.2 h) with increasing ratios of AF wastewater, while the PDA-UASB utilized organic carbon from domestic sewage to simultaneously remove nitrate (91.7 mg/L) and ammonium (66.3 mg/L) through the PDA pathway (HRT=11.3 h). After 200 days, the relative abundance of Candidatus Brocadia increased from 0.6 to 5.4 %, ensuring robust nitrogen removal performance. The system achieved effluent ammonium and total nitrogen concentrations below 1.0 mg/L and 15.0 mg/L, respectively, with a total nitrogen removal rate of 87 %. This study demonstrates the N-PDA process as an efficient, energy-saving, and cost-effective solution for treating AF wastewater without the additional organic carbon sources, enhancing nitrogen removal efficiency and operational stability.

Enhancing nitrogen and phosphorus removal in plant-biochar-pyrite stormwater bioretention systems: Impact of temperature and high-frequency heavy rainfall

Global climate change and rapid urbanization have resulted in more frequent and intense rainfall events in urban areas, raising concerns about the effectiveness of stormwater bioretention systems. In this study, we optimized the design by constructing a multi-layer filler structure, including plant layer, biochar layer, and pyrite layer, and evaluated its performance in nitrogen (N) and phosphorus (P) removal under different temperatures (5–18 °C and 24–43 °C), rainfall intensity (47.06 mm rainfall depth), and frequency (1–5 days rainfall intervals) conditions. The findings indicate that over 775 days, the plant system consistently removed 62.3% of total nitrogen (TN) and 97.0% of total phosphorus (TP) from 103 intense rainfall events. Temperature fluctuations had minimal impact on nitrate nitrogen (NO3−-N) and TP removal, with differences in removal rates of only 1.0% and 0.6%, respectively, among plant groups. Across the multi-layer structure, plant roots mitigated the impact of temperature differences on NO3−-N removal, while high-frequency rainfall fluctuated the stability of NO3−-N removal. Dense plant roots reinforced N and P removal by facilitating denitrification in the vadose zone (biochar) and strengthening denitrification processes. Biochar and pyrite contributed to stable microenvironments and diverse ecological functions, enhancing NO3−-N and PO43− removal. In summary, the synergistic effects of the multi-layer filler structure improved and stabilized N and P removal, providing valuable insights for addressing runoff pollution in bioretention systems amidst rapid urbanization and climate change challenges.

Optimizing operation strategy to improve storage of intracellular carbon sources in anaerobic/oxic/anoxic system: Enhanced nitrogen removal by endogenous denitrification

Endogenous denitrification (ED) can make full use of the carbon sources and avoid replenishment of it. However, strengthening the storage of intracellular carbon sources is an important factor in improving ED efficiency. In this study, employed batch experiments using real domestic wastewater in the anaerobic/oxic (A/O) process. The anaerobic and oxic processes were run for 4 h under ambient conditions with the dissolved oxygen (DO) concentrations in the oxic stage controlled at 0.5, 1.0, 1.5, and 3.0 mg/L, respectively. The results showed that the content of poly-β-hydroxyalkanoates (PHA) reached its peak at 60 min (1.25 mmolC/L). And with DO concentrations of 1.5 mg/L, the contents of glycogen (Gly) were 27.74 mmolC/L. Subsequently, the AOA-SBR was established to investigate its effect on the long-term nitrogen removal performance of domestic wastewater by optimizing the anaerobic time and DO concentrations. The results showed that at an anaerobic time of 60 min and DO concentration of 1.5 mg/L, the storage of the intracellular carbon sources was highest and the total nitrogen (TN) removal efficiency increased to 82.12%. In addition, Candidatus Competibacter dominated gradually in the system as the strategy was optimized.

Enhancing energy and nitrogen removal efficiency through automatic split injection and innovative aeration device: A study of low C/N ratio environments

A new aeration device based on Bernoulli's principle, Jetventrumixer (JVM), was introduced into an aeration tank in denitrification process, which involved an automatic split injection system (ASIS) into two denitrification tanks every 10 minutes. Real-time monitoring of influent water allowed the calculation of the C/N ratio, enhancing the utilization efficiency of internal carbon sources while reducing the need for external carbon. The comparison of the JVM with the conventional air diffuser for 100 days operation showed that the removal efficiency for NH4+-N in both systems was approximately 98 %, but the nitrification efficiencies were 84 % and 80 %, respectively. This indicates that the JVM achieves an high enough removal efficiency and nitrification efficiency compared with conventional air diffuser system with dramatic reduction in energy consumption by 52.1 %. When the influent wastewater was split and injected into duplicate denitrification tanks at ratios of 3:7, 5:5, and 7:3, the total nitrogen (TN) removal efficiencies were 77 %, 73 %, and 72 %, respectively. In contrast, with the implementation of the ASIS, the TN removal efficiency increased up to 82 %. The increase in TN removal indicates that real-time monitoring could stably track changes chemical composition in wastewater influent over 24 h and introducing ASIS facilitate the efficient utilization of internal carbon sources, thereby enhancing denitrification efficiency and improving TN removal efficiency. Finally, the greenhouse gas (GHG) emissions from the JVM and air diffuser were 9.39401 and 19.60488 tCO2eq year-1, respectively, representing a 52% reduction. Therefore, JVM and ASIS successfully reduced energy consumption and enhanced both nitrification and denitrification efficiencies.