Introduction Selection of endurance test for cyclist, which reflects most precisely training or starting is very complicated. Accurate evaluation of endurance warrants pricise indication of training load (Lucia et all 2004). Alteration of training load is pricesly correlated with periodization of training load (RA¸nnestad, Hansen 2014) and is result of various training goals set for respective traning periods (RA¸nnestad et all 2014). Purpose of this study was establishing training load during general and special preparatory periods in cross country cyclists. Double testing procedurA™ for endurance testing was used to verify different intensity zone parameters.  Methods Subjects for this study were cyclits MTB Cross Country team. Women (n=3), age: 28,5±2,3 years old, body mass: 54,1±2,2 kg, body height: 171,8±2,4 cm and men cyclists (n=5), age: 29,3±4,8 years old, body weight: 64,1±4,7 kg, body height: 178,6±3,4 cm, All studies were medalists of Olimpic Games, World and Europe Championships Test were performed at the beginning of 12-week general and special preparatory period. The participants of the study performer graded incremental exercise test (GXTs). The GXTs test was executed on ergometer Cyclus 2 (RBM, Germany). The I-st step was 1Wxkg-1 b.m. and increased every 3 minutes by 0,5 Wxkg-1 b.m. In last 30 the seconds of every exercise grade was taken 20 µl of arterialized blood to the sign LA (Biosen S-line, EKF, Germany). In the course of effort VO2, VE, VCO2 was measured by means of K4b2 analyser. The heart rate monitor, Polar V650 (Polar Finland ) measured HR during GXTs. At the level of aerobic threshold (LT) (Farell et al. 1979), anaerobic threshold (AT) (Powers et al. 1983) i VO2max. the power value was set. After this subjects performer 60-minute effort characterized by: 10minutes at 50% PVO2max, 5 minutes at 75% PVO2max, 5 minutes at 90% PVO2max, 10 minutes at 50% PVO2max, 10 minutes at 75% PVO2max, 5 minutes at 90% PVO2max, 6 minutes at 75% PVO2max, 5 minutes 50% PVO2max and 4 minutes at 100% PVO2max.  Results In table 1 presented are results of graded incremental endurance test. Table 2 presents results of 60-minutes variable-intensity endurance test. This efforts reflects loads of special training based on intensity and proportions which is used in cross country cycling. It allows to evaluate changes in endurance as results of applied training load. In both tested groups it was observed during graded test statistically significant increase (p≤ .001 - 0.005) of VO2max induced during preparatory period. Other parameters i.e. HRmax and HR at aerobic and anaerobic threshold has not been changed significantly. Analysis of maximal values obtained during 60-minute test revealed reduced values of maximal LA, VE and HR after general preparatory period. With constant intensity in each preparatory period it was observed adaptation of energy system contribution to performer affort. Partially anaerobic effort was replaced by aerobic effort. Results of this study shown that first 6 weeks of preparatory period improved aerobic capacity and follwoing 6-week period increased level of anaerobic values.  Conclusions 60-minute variable effort has served 2 planned in this study purposes. It has evaluated direction and range of changes in subsequent preparatory periods on following values: VO 2 max, VEmax i HRmax. Intensity zones after general preparatory period are too low to evaluate effectivness of training for general and special preparatory period. Training efforts lasting 60 minutes consisting of: 21 minutes at 50% PCr, 26 minutes at 75% PCr, 10 minutes at 90% PCr and 3 minutes at intensity of 100% PCr allows to observe effort which reflects specific cross country cycling effort. It also allows observe changes in endurance parameters during preparatory period. In order to use this test for purpiose of verification of training intensity zones, it is required to performing test and establishing parameters used in graded endurance test.