A 24′′ (610 mm) access laser-Doppler velocimeter (LDV) system was developed to make simultaneous three-velocity-component measurements in a low speed linear cascade wind tunnel with moving wall simulation. The probe has a 610 mm access length and achieves a measurement spatial resolution of 100 μm by using off-axis optical heads. With the relatively large access length, the LDV probe allows for measurements from the side of a wind tunnel instead of through the tunnel floor, while the high spatial resolution allows for quality near-wall measurements. The probe has been tested in a zero-pressure gradient 2D turbulent boundary layer and the test results agree well with the experimental data measured with different LDV systems and hot-wire anemometery for the boundary layer flows. The energy spectral density was estimated using a slot correlation, and Von Karman’s model for the energy-spectrum function was used to analyze the measured spectral data to estimate the turbulent kinetic energy dissipation rate, which compares favorably with the measured production values in the log-layer region of the turbulent boundary layer. Measurements are presented for the moving endwall boundary layer at the inlet of the linear compressor cascade facility to validate the capability of this LDV for tip leakage flow measurements. These results indicate that the moving endwall reduces velocity gradients in the near-wall region and results in less production of Reynolds stresses and turbulent kinetic energy compared to the stationary endwall case.