Electrical Resistivity Values Research Articles

Capsaicin Reduces Obesity by Reducing Chronic Low-Grade Inflammation.

Chronic low-grade inflammation (CLGI) is associated with obesity and is one of its pathogenetic mechanisms. Lipopolysaccharide (LPS), a component of Gram-negative bacterial cell walls, is the principal cause of CLGI. Studies have found that capsaicin significantly reduces the relative abundance of LPS-producing bacteria. In the present study, TRPV1-knockout (TRPV1-/-) C57BL/6J mice and the intestinal epithelial cell line Caco-2 (TRPV1-/-) were used as models to determine the effect of capsaicin on CLGI and elucidate the mechanism by which it mediates weight loss in vivo and in vitro. We found that the intragastric administration of capsaicin significantly blunted increases in body weight, food intake, blood lipid, and blood glucose in TRPV1-/- mice fed a high-fat diet, suggesting an anti-obesity effect of capsaicin. Capsaicin reduced LPS levels in the intestine by reducing the relative abundance of Proteobacteria such as Helicobacter, Desulfovibrio, and Sutterella. Toll-like receptor 4 (TLR4) levels decreased following decreases in LPS levels. Then, the local inflammation of the intestine was reduced by reducing the expression of tumor necrosis factor (TNF)-α and interleukin (IL)-6 mediated by TLR4. Attenuating local intestinal inflammation led to the increased expression of tight junction proteins zonula occludens 1 (ZO-1) and occludin and the restoration of the intestinal barrier function. Capsaicin increased the expression of ZO-1 and occludin at the transcriptional and translational levels, thereby increasing trans-endothelial electrical resistance and restoring intestinal barrier function. The restoration of intestinal barrier function decreases intestinal permeability, which reduces the concentration of LPS entering the circulation, and reduced endotoxemia leads to decreased serum concentrations of inflammatory cytokines such as TNF-α and IL-6, thereby attenuating CLGI. This study sheds light on the anti-obesity effect of capsaicin and its mechanism by reducing CLGI, increasing our understanding of the anti-obesity effects of capsaicin. It has been confirmed that capsaicin can stimulate the expression of intestinal transmembrane protein ZO-1 and cytoplasmic protein occludin, increase the trans-epithelial electrical resistance value, and repair intestinal barrier function.

Integrative approach to enhance the soft magnetic properties of Co35Cr5Fe10Ni30Ti20-xAlx (x= 10, 15, 20) high entropy alloys

High Entropy Alloys (HEAs) are advanced materials having multi-functional properties. These materials have the potential to overcome the shortcomings of conventional soft magnetic alloys. In this study, an integrative approach is adopted to enhance the magnetic and electrical properties of CoCrFeNiTi-based HEA by the addition of Al in different content for Ti and annealing for different temperatures and durations. Firstly, Co35Cr5Fe10Ni30Ti20-xAlx (x= 10, 15, 20) HEAs were synthesized through mechanical alloying and investigated for phase formation and magnetic properties. Major fcc with minor bcc and intermetallic R phase has formed for Co35Cr5Fe10Ni30Ti10Al10 and Co35Cr5Fe10Ni30Ti5Al15 HEAs. However, the mixture of fcc and bcc phase with a minor fraction of σ phase has been found for Co35Cr5Fe10Ni30Al20 HEA. The value of Ms is slightly decreased for Co35Cr5Fe10Ni30Ti5Al15 HEA as compared to Co35Cr5Fe10Ni30Ti10Al10 HEA, but for Co35Cr5Fe10Ni30Al20 HEA, the value of Ms has drastically decreased when Ti completely removed for Al. Among these three synthesized HEAs, the highest value of Ms is found for Co35Cr5Fe10Ni30Ti10Al10 HEA (Ms=78 emu/g). Further, we investigated the effect of annealing at different temperatures for 2 h on phase evolution, microstructure, and their correlation with magnetic properties of Co35Cr5Fe10Ni30Ti10Al10 HEA. Among annealed HEA, 700°C annealed Co35Cr5Fe10Ni30Ti10Al10 HEA has high Ms (104 emu/g), and the value of Hc was found to be 29 Oe. The long-duration annealing is also performed at 700°C for 10 h to improve the magnetic properties of the as-synthesized Co35Cr5Fe10Ni30Ti10Al10 HEA and found good soft magnetic behaviour (Ms=103.5 emu/g and Hc=13 Oe). Moreover, the electrical resistivity of the long-duration annealed Co35Cr5Fe10Ni30Ti10Al10 HEA is also recorded in the temperature range 2–350 K, and the value of electrical resistivity at 2 K and 300 K is found to be 180.2 ×102 µΩ.cm and 200.80 ×102 µΩ.cm, respectively. The found value of electrical resistivity is the highest value of resistivity reported so far in the case of soft magnetic HEA.

Temperature dependent electrical resistance and mesoscopic electronic transport mechanisms on aerographite and single-walled carbon nanotube aerogel

We investigate temperature dependent electrical resistance properties of aerographite and single-walled carbon nanotube (SWCNT) aerogel in the temperature range of 2–300 K by employing the four-probe method with magnetic field effects (in the range 0–9 T, in steps of 2 T). The current–voltage (I–V) curves were taken for several temperatures varying from 5 to 300 K, and the electrical resistance values of aerographite and SWCNT aerogel were decreased from 7.30 Ω (5 K, 0 T) to 4.88 Ω (300 K, 0 T) and 22.56 Ω (5 K, 0 T) to 0.99 Ω (300 K, 0 T) with temperature increases, respectively. Experimental results show that the electrical resistance falls exponentially as the temperature increases. Such temperature dependence of R(T) points to a form of tunneling conduction or hopping. Two mesoscopic mechanisms for electronic transport, fluctuation-induced tunneling conduction (FITC) and variable range hopping (VRH), are employed to explicate possible electrical conduction mechanisms occurring in aerographite and SWCNT aerogel, respectively. These mainly result in disorder-induced symmetry-breaking, which are modified by their structural symmetries and electronic band structures, both play important roles in temperature dependent electrical resistance properties of aerographite and SWCNT aerogel. Characteristic parameters (T0, T1, and R0) have been estimated using the morphology and the uncertainty principle for aerographite and the percolation theory for SWCNT aerogel. While the FITC mechanism captures a wide temperature range of data for aerographite, the VRH model provides an explanation for SWCNT aerogel. This study provides groundwork for further development of carbon aerogel systems with high conductivity in large-scale preparation.

Effect of heat treatment on microstructure of near-eutectic Al-Ni-Mn alloy, and determination of mechanical and thermoelectrical properties

This study examined the impact of solution heat treatment on the microstructure, mechanical characteristics, thermophysical properties, and electrical resistivity of an Al-Ni-Mn near-eutectic alloy. The investigation focused on varying temperatures and holding periods. The composition of the Al-Ni-Mn near-eutectic alloy system was chosen as Al-5.3%Ni-1.0%Mn (wt). In the non-heat-treated sample, the matrix phase (α-Al) is in equilibrium with the intermetallic Al9(Mn,Ni)2 and Al3Ni phases. The hardness value of the non-heat-treated sample (49.8 kg mm−2) increased to 70.1 kg mm−2 with 2 h of solution heat treatment at 570 °C and then 8 h of artificial aging at 180 °C. The hardness value increased by approximately 41%. TE: 651.81 °C for the non-heat-treated sample and TE:648.79 °C for the heat-treated sample. Fusion enthalpy (ΔH) value was determined as 336.79 (J g−1) for the non-heat-treated sample and 516.36 (J g−1) for the heat-treated sample. Heat Capacity (Cpl) value was found to be 0.364 J g−1.K for the non-heat-treated sample and 0.560 J g−1.K for the heat-treated sample. The electrical resistivity value of the 2 h’ solution heat-treated sample at 600 °C reached its highest value.

Investigating the Effect of Weave Type and Filament Count on Electrical Conductivity of Polyethylene Terephthalate (PET) Fabrics Coated with PEDOT Polymer

The rapid development of smart and wearable textiles has been driven by the need for smaller and lighter electronic circuits. To make textile surfaces conductive, various methods have been developed, with vapor-phase polymerization being a preferred method due to its smoothness, conductivity, and ease of application. This study investigates the effect of fundamental characteristics of textile fabrics, such as weave type and filament count, on electrical conductivity. Fabrics woven with different filament counts and weave types were coated with PEDOT polymer using vapor-phase polymerization. The results showed that the fabric woven with 150F288 yarns and a 3/1 twill weave exhibited lower electrical resistance, attributed to the microfibrous structure of the yarn and the twill's staggered structure. Despite increases in resistance values after performance tests, the electrical resistance values remained within a sufficient conductivity range. This research contributes to the understanding of how fabric characteristics affect the electrical conductivity of coated textiles, paving the way for the development of smart electronic textiles.

Protection of Tight Junctional Complexes between hCMEC/D3 Cells by Deep-Sea Fibrinolytic Compound FGFC1.

Tight junctional complexes (TJCs) between cerebral microvascular endothelial cells (CMECs) are essential parts of the blood-brain barrier (BBB), whose regulation closely correlates to the BBB's integrity and function. hCMEC/D3 is the typical cell line used to imitate and investigate the barrier function of the BBB via the construction of an in vitro model. This study aims to investigate the protective effect of the deep-sea-derived fibrinolytic compound FGFC1 against H2O2-induced dysfunction of TJCs and to elucidate the underlying mechanism. The barrier function was shown to decline following exposure to 1 mM H2O2 in an in vitro model of hCMEC/D3 cells, with a decreasing temperature-corrected transendothelial electrical resistance (tcTEER) value. The decrease in the tcTEER value was significantly inhibited by 80 or 100 µM FGFC1, which suggested it efficiently protected the barrier integrity, allowing it to maintain its function against the H2O2-induced dysfunction. According to immunofluorescence microscopy (IFM) and quantitative real-time polymerase chain reaction (qRT-PCR), compared to the H2O2-treated group, 80~100 µM FGFC1 enhanced the expression of claudin-5 (CLDN-5) and VE-cadherin (VE-cad). And this enhancement was indicated to be mainly achieved by both up-regulation of CLDN-5 and inhibition of the down-regulation by H2O2 of VE-cad at the transcriptional level. Supported by FGFC1's molecular docking to these proteins with reasonable binding energy, FGFC1 was proved to exert a positive effect on TJCs' barrier function in hCMEC/D3 cells via targeting CLDN-5 and VE-cad. This is the first report on the protection against H2O2-induced barrier dysfunction by FGFC1 in addition to its thrombolytic effect. With CLDN-5 and VE-cad as the potential target proteins of FGFC1, this study provides evidence at the cellular and molecular levels for FGFC1's reducing the risk of bleeding transformation following its application in thrombolytic therapy for cerebral thrombosis.

Nebulized spray pyrolysis deposited CdO thin films for liquefied petroleum gas and ethanol gas sensor

High quality cadmium oxide (CdO) thin films were prepared through an inexpensive and simple nebulized Spray Pyrolysis (neb-SP) technique. The transparent conducting CdO thin films deposited at different substrate temperatures (TS) for the ethanol and liquefied petroleum gas (LPG) sensor application. XRD, SEM, UV–vis Spectroscopy and Hall Effect were done to study the structural, morphological, optical and electrical properties respectively. Polycrystalline nature thin films shows (1 1 1) cubic structure as the prominent peak. Surface morphology studies reveal that the grain size and roughness of the films are increased with increase in the substrate temperature. The average transmittance of the yellowish transparent CdO thin films is about 57%, thus the films are transparent in the visible region. The optical band gap (Eg) value of the CdO films increased from 2.16 to 2.28 eV with increase in the TS from 225 °C to 300 °C. The minimum value of electrical resistivity of the films is 1.40 × 10−3 Ωcm obtained at 275 °C. The neb-SP deposited CdO thin film (275 °C) shows maximum response to the ethanol and LPG gas sensitivity of 31.87% and 16.4% respectively at operating temperature of 573 K. Thus the results authenticate that the CdO thin films deposited low substrate temperature with high quality can be achieved via neb-SP technique compared to other wet chemical or physical method.

Evaluation of the possibility of searching for groundwater using electrical resistivity tomography based on the data of numerical modelling in complicated permafrost conditions (the case of the territory in the eastern part of the Republic of Buryatia)

Relevance. Groundwater is the main source of industrial and technical water supply for mining facilities. The geological structure of the studied areas can be complicated by various factors that make it difficult to search for and localize groundwater. In this regard, electrical geophysical prospecting methods are often included in the complex of prospecting and exploration works, before which it is necessary to justify the application of the chosen method for solving the problems by performing numerical modelling. Aim. To substantiate the use of electrical resistivity tomography for the search for groundwater, taking into account complicating factors, including perennially frozen rocks, taliks, and fault zone, by numerical modelling. Objects. Geological environment represented by Quaternary system rocks and permafrost rocks of Cretaceous sediments of the Zazinskaya intermontane area, fault zone, talik zones. Methods. Solution of the direct problem, solution of the inverse problem, ground geophysical surveys using electrical resistivity tomography. Results. Based on the performed numerical modelling, it is shown that the use of electrical resistivity tomography is expedient when searching for groundwater in the area characterized by various factors: permafrost, talik, and a fault zone. Based on the results of solving direct and inverse problems of electrical survey, using field works performed in 2020 in the Eravninsky district of the Republic of Buryatia as an example, it is shown that pole-dipole arrays in electric resistivity survey with electrode distance of 5 m, successfully confirmed by drilling operations, allow us reliably delineate potential zones of water inflow. These zones, characterized by low values of electrical resistivity, in this area, are related to talik zones, which are powered by heat flow from the bowels of the earth.

Remote Detection of Leakages from a Compromised Buried Water Supply Pipe through Geophysical Measurements

The continuous drastic loss of stored water at a private household presumed to be caused by leakage(s) was subjected to geophysical investigations with a view to delineating the source(s) of the leakage(s). The Spontaneous Potential (SP) and Electrical Resistivity (ER) methods were deployed for the study. Measurements were taken along four (4) traverses at two intervals – firstly when the water supply valve from the overhead storage tank was closed and secondly when it was opened. The Total Field array was adopted for SP measurements with constant station separation of 0.5 m. ER measurements were taken via the Wenner array with electrode spacing (a) varied with an interval of 0.5 m from 0.5 m to 1.5 m. SP data were presented as profiles and maps while ER data were presented as 3-D resistivity depth stacks. SP values varied from -100 mV to 350 mV during the two measurement periods. ER values also varied from 100 ohm.m to 1200 ohm.m during the closed valve period and 100 ohm.m to 900 ohm.m during the opened valve period. During the closed valve period, pipeline routes were interpreted as manifesting oval-shaped high potential anomalies with central linear trend on the SP map; and as linearly-trending high resistivity intrusion within low resistivities on resistivity maps. The disparities between the results of closed valve and opened valve measurements were pointers to leakage-induced inhomogeneity in the subsurface. The diminished high potential SP anomaly and the anomalously low resistivity specks within linearly-trending high resistivity values were regarded as the source of leakage.

Influence of multi-doping (Zn and Sn) on the optical, and electrical properties of spray-deposited CdO thin films

This investigation has been focused on the spray-deposited pure cadmium oxide (CdO) thin films and also doped with zinc (Zn) and tin (Sn), as a transparent conducting oxide for optoelectronic applications. By varying the concentration of Sn (0–2%) into CdO, the microstructural, morphological, and optoelectronic properties were investigated. XRD spectra confirmed the polycrystalline cubic structure of the pure, doped, and co-doped CdO films. SEM investigation reveals the crack-free homogeneous cauliflowers-like morphology of the fabricated thin films. The existence of elemental compositions such as Cd, Zn, Sn, and O in the prepared films was confirmed by EDS analysis. All of the doped and co-doped CdO films showed good transparency in the visible range. Moreover, a slight increment has been found in the optical band gap from 2.43 eV to 2.50 eV with increasing Sn concentration, which can be explained by the Burstein-Moss phenomena. A decrement of Urbach energy was noted for an increased concentration of Sn. Besides, Zn and Sn (1 %) co-doped films exhibited the lowest electrical resistivity value 4.09×10−4 Ω-cm among other doped compositions. However, the addition of Zn and Sn into CdO thin films enhanced the optoelectronic properties.

Electrical resistivity of eco-friendly hybrid fiber-reinforced SCC: Effect of ground granulated blast furnace slag and copper slag content as well as hooked-end fiber length

Over the last 20 years, the development of electrically conductive composites for removing snow and ice from transportation infrastructure has received exceptional traction. However, these composites need to exhibit stable electrical conductivity and high mechanical properties to be sustainable and cost-effective. Towards this goal, the article investigates the roles of ground granulated blast furnace slag (BFS) and copper slag (CS) content, in addition to hooked-end steel fiber length, on the electrical properties of eco-friendly ultra-high performance hybrid fiber-reinforced self-compacting concrete (HFR-SCC) for the first time in the literature. For this purpose, sixteen eco-friendly electrically conductive ultra-high-performance HFR-SCC were designed based on the variable parameters of four different BFS/total binder ratios (20, 40, 60, and 80 %), a CS/total fine aggregate ratio of 50 %, and two different hooked-end fiber lengths (30 and 60 mm), while all mixes used 1.75 % by volume fraction of steel fibers. After determining the workability properties (slump-flow and T500 values) of all mixes, compressive strength and electrical resistivity/conductivity tests of 90-day specimens were conducted. Additionally, environmental and economic evaluations of all mixes in terms of sustainability were performed in order to clarify the effects of the variable parameters. Taking into account the experimental results obtained, it was observed that all electrically conductive ultra-high performance HFR-SCC mixes demonstrated satisfactory workability properties, while the compressive strength values reached to impressive values of 127 MPa. The optimum BFS/total binder ratio was identified to be 40 % for higher compressive strength and conductivity of ultra-high performance HFR-SCC specimens. On the other hand, the addition of CS to the mixes resulted in an increase of almost 9 % in compressive strength compared to one without CS, while at the same time, a significant increase of approximately 363 % was observed in the electrical conductivity values of the specimens. As for the influence of different lengths of hooked steel fibers, the use of 30 mm length hooked-end steel fibers in HFR-SCC mixes performed better in terms of compressive strength, whereas 60 mm fibers performed better regarding electrical conductivity. In conclusion, this experimental work has evidenced that it is possible to develop an eco-friendly and sustainable electrically conductive ultra-high performance cementitious composite (the optimal mix compressive strength and electrical resistivity values were 127 MPa and 2242 Ω.cm, respectively) by using waste from different industries such as iron and copper. Thus, it will provide important insights for the design and application of future electrically conductive concretes, which can be an important alternative in efficient active deicing and snow-melting applications.

The influence of heavy doping of TiCoSb intermetallic semiconductor with Cr atoms on structural, kinetic and energetic properties

The structural, electrokinetic, and energetic properties of the TiСо1-xCrxSb semiconductor obtained by doping TiCoSb with Cr atoms introduced into the structure by substituting Co atoms in the crystallographic position 4c were studied. It was shown that in TiСо1-xCrxSb the structural defects of donor and acceptor nature are generated simultaneously in different ratios depending on the impurity concentration. At concentrations of х ≥ 0.02, the conductivity of TiСо1-xCrxSb has a metallic character, and the contribution of current carrier scattering mechanisms to the value of electrical resistivity is of the same order as changes in the concentration of current carriers. It was established that at all temperatures in the range of concentrations x = 0–0.02, the rate of generation of donors exceeds the rate of generation of acceptors, and at concentrations x > 0.02, on the contrary, the rate of generation of acceptors is greater than that of donors. This is indicated by the positive values of thermopower coefficient α(х,Т) of TiСо1-xCrxSb for х > 0.03.

One-pot synthesis of Li3xLa2/3−xTiO3/PVA composite with high tunable electrical attributes governed by the Li+ content

The primary goal of this study is to elucidate the structural and electrical characteristics, alongside potential applications, of ceramic-polymeric composites. These composites are achieved by incorporating lithium-lanthanum titanates (Li3xLa2/3−xTiO3 - LLTO) into a polyvinyl alcohol polymeric matrix (PVA). The synthesis of lithium-lanthanum titanates occurred through high-energy ball milling method. Then, three types of composites were produced using the solvent casting method: a sample of pure Poly (vinyl alcohol) polymer (PVA), a second PVA composite with the addition of La0·59Li0·24TiO3 (Li-0.24) and a third sample with La0·56Li0·33TiO3 (Li-0.33). SEM and AFM analyzes revealed notable changes in the morphology and 3D spatial patterns of the films upon incorporating lithium-lanthanum titanates into the PVA matrix. The PVA film presents a topography featuring a more evenly distributed range of topographic heights, indicating a smoother surface structure. With varying LLTO content, the morphology of the PVA composite underwent changes. The lower roughness of the PVA surface is evident from its measured average roughness value (Ra = 1.9 ± 0.5 nm). Upon incorporating LLTO, the surfaces displayed increased roughness, measuring specifically at 40.1 ± 7.3 nm (Li-0.24) and 22.7 ± 5.5 nm (Li-0.33). Notably, there was an approximately 43 % reduction in the average roughness value from Li-0.24 to Li-0.33. The analysis by Raman allowed the identification of functional groups of the PVA polymeric chain, in addition to the presence of some vibrational modes related to the structure of lithium-lanthanum titanates for the sample with the highest content of Li+ ions (Li-0.33). Finally, the sample Li-0.33 showed the lowest value of electrical resistance (67 kΩ) and of phase angle (16°), compared to the PVA (100 MΩ and 90°). These results show that the presence of the LLTO changed the insulating behavior of the PVA matrix changed and improved potential to electronic applications.

تقييم الأداء الديناميكي لتكوين الحواز في حوض مرزق بليبيا كما يستدل عليه بتقنية التفسير السريع.

The evaluating of the Hawaz reservoir in the Murzuq basin based on the well recording data available for twelve exploratory were provided for this study. These records data have been examined through employing some cross-plots and use their outputs for controlling the interactive petrophysics software in order to identify the lithological constituents and fluid saturation parameters.The litho-saturation cross-plot indicated that the Hawaz Formation consists of sandstones with few shale.The Hawaz formation officially subdivided intoeight horizons, namely H1–H8. Horizons (H4 and H5) are the main reservoirs in Hawaz formation as all readings indicate its high resistivity, with positive differentiation between its different curves, high porosity, and low gamma ray. BVW min technology is introduced the fast method depends on calculating the minimum total water volume so that the reservoir is considered to contain irreducible water, meaning that water will not be produced with the oil. The value of BVW min was 0.05, meaning that any range for which the product of porosity multiplied by water saturation (Φ.SW) is greater than that value is excluded from any detailed calculations.Also, calculate the minimum value of electrical resistance (Rtmin=140ῼm) for oil production zones, where zones with electrical resistivity less than that value are excluded. Keywords: Hawaz Formation, H field of NC186, petrophysical parameters, well logging.

Electrodeposition of Thin Silicon Films for Neutron Transmutation Doping

Thin silicon films were electrodeposited on glassy carbon (GC) from the KF-KCl (2:1)—75 mol% KI—1.5 mol% K2SiF6 melt under potentiostatic condition at 973 K. The synthesized films were single-phase, continuous, dense, and free from unwanted impurities. Neutron transmutation doping (NTD) of the samples was performed in the IVV-2M research reactor (RR) at a thermal neutron flux density of 1.8 × 1013 cm−2s−1 for 7.7 h in order to form the 31P isotope dopant. The irradiated samples were studied by scanning electron microscopy with energy-dispersive X-ray spectroscopy, X-ray diffraction, mass spectrometry, and gamma-ray spectrometry. Some excess of the minimum significant specific activity of the irradiated samples was explained by the formation of the 182Ta isotope due to the presence of tantalum traces in the GC substrate. The formation of the 31P isotope by the NTD process was confirmed. The calculated values of 31P concentration and electrical resistivity were 4.9 × 1016 cm–3 and 0.15 Ω·cm, respectively.

The effect of different degree of compaction towards electrical resistivity value for cohesive soil

The effect of different degree of compaction towards electrical resistivity value for cohesive soil

Механизм влияния электрического поля на процесс модифицирования серого чугуна

A diagram of an experimental installation for measuring the electrical resistance of a gray cast iron melt is presented, where a gauge based on a Kelvin double bridge is used. It is shown that the value of the specific electrical resistance of gray cast iron varies depending on the type of conditioning agent. Experimental data on the difference in specific resistivity values for various gray cast iron nucleating agents are presented. Changes in the values of electrical resistance under the influence of electric field treatment of inoculated cast iron melt are also given. Comparison of the strength values of gray cast iron with its values of the electrical resistivity of the cast iron melt after the electrical field exposure showed their simultaneous change. The decrease in the values of the electrical resistance of hot iron due to the electric field exposure is shown and it corresponds to an increment in the strength of inoculated cast iron. The effectiveness of the electric field exposure on the process of inoculation is determined by thermal ionization of the chemical elements included in the conditioning agent. The maximum increase of these parameters after electric field exposure when using FS75 nucleating agent is shown. The mechanism of the influence of an electric field on the cast iron solidification for the inoculation process of FS75, containing 75 % silicon in its composition, is described. The dependence of the efficiency of electric field exposure on the composition of the nucleating agent for gray cast iron is shown. A physical model of the effect of an electric field exposure on the process of cast iron inoculation is found. The possibility of influencing the hydrodynamic situation in the melt of gray cast iron and changing the rate of dissolution of nucleating agent particles by means of electric field exposure is given

Exploring the therapeutic potential of cinnamoyl derivatives in attenuating inflammation in lipopolysaccharide-induced Caco-2 cells.

Aim: In gastrointestinal (GI) diseases, lipopolysaccharide (LPS) exacerbates gut-barrier dysfunction and inflammation. Cinnamoyl derivatives show potential in mitigating LPS-induced inflammation.Materials & methods: We assessed intestinal epithelial barrier function using Trans-epithelial electrical resistance values and measured inflammatory mediators through real-time PCR and ELISA in Caco-2 cells.Results: LPS treatment increased IL-6, IL-1β, TNF-α, PGE2 and TRL4 expression in Caco-2 cells. Pre-treatment with DM1 (1 or 10μM) effectively countered LPS-induced TLR4 overexpression and reduced IL-6, IL-1β, TNF-α and PGE2 levels.Conclusion: DM1 holds promise in regulating inflammation and maintaining intestinal integrity by suppressing TLR4 and inflammatory mediators in Caco-2 cells. These findings suggest a potential therapeutic avenue for GI diseases.

Comparative assessment of modern methods of diagnosis of subclinical mastitis in cows

Diseases of the mammary gland, among which mastitis is the most common, cause significant damage to dairy farming. On different farms, from 3 to 50% of lactating cows are sick, and only in 30% of cases the disease manifests itself clinically, and in most cases it has a subclinical course. Early diagnosis of the subclinical stage of mastitis is of great economic, sanitary and technological importance. The effectiveness of timely treatment of sick animals and prevention of the clinical stage of mastitis and ensuring normal milk quality largely depends on it. The study was conducted at the National Research Center of the Ukrainian National Academy of Sciences on 92 lactating cows of the Ukrainian black-spotted breed. Each animal was diagnosed with clinical and subclinical mastitis. Research on subclinical mastitis was carried out using the German milk test and determining the electrical resistance of milk. The diagnostic value of these methods of settling breakdown was monitored. According to the results of the standing test, subclinical mastitis was detected in 30 (32.6%) of 92 lactating cows during the study (49 quarters were affected). Indirect determination of the number of somatic cells using the German milk test allows rapid diagnosis of subclinical mastitis with an accuracy of 84.8%. The use of the "Electronic determinant of mastitis in cows" with the interpretation of the obtained results according to the value of electrical resistance or the difference between the indicators of individual quarters according to the instructions ensures the accuracy of the result in 82.6 and 91.3% of cases, respectively. It is advisable to interpret the indicators of electrical resistance of milk taking into account both indicators. At the same time, cows with indicators of the numerical value of the electrical resistance of milk of 340 (u).o. should be considered healthy. and more and with a difference between the indicators of the quarters of 50 (u).o. and less; for sick animals, the electrical resistance of the secretion of the mammary gland in the range of 260 (u).o. is characteristic. And less with a difference between the largest and smallest indicators of individual quarters of 100 (u).o. and more. A diagnosis of subclinical mastitis can be considered doubtful if the electrical resistance of milk is between 270 and 330 (u).o. and differences between indicators of quarters 60 - 90 (u).o. Key words: cows, subclinical mastitis, electronic mastitis detector, EIMU.

GPR30 alleviated subarachnoid hemorrhage-induced blood-brain barrier dysfunction by activating the PI3K/Akt and Nrf2/HO-1 pathways.

The blood-brain barrier (BBB) plays a critical role in the development and outcome of subarachnoid hemorrhage (SAH). This study focuses on the potential mechanism by which G-protein-coupled estrogen receptor 30 (GPR30) affects the BBB after SAH. A rat SAH model was established using an intravascular perforation approach. G1 (GPR30 agonist) was administered to investigate the mechanism of BBB damage after SAH. Brain water content, Western blotting, Evans blue leakage, and immunofluorescence staining were performed. Brain microvascular endothelial cells were induced by hemin to establish SAH model in vitro. By adding LY294002 [a phosphatidylinositol 3-kinase (PI3K) blocker] and zinc protoporphyrin IX (ZnPP IX) [a heme oxygenase 1 (HO-1) antagonist], the mechanism of improving BBB integrity through the activation of GPR30 was studied. In vivo, GPR30 activation improved BBB disruption, as evidenced by decreased cerebral edema, downregulated albumin expression, and reduced extravasation of Evans blue and IgG after G1 administration in SAH rats. Moreover, SAH downregulated the levels of tight junction (TJ) proteins, whereas treatment with G1 reversed the effect of SAH. The protective effect of G1 on BBB integrity in vitro was consistent with that in vivo, as evidenced by G1 reducing the impact of hemin on transendothelial electrical resistance (TEER) value, dextran diffusivity, and TJ protein levels in brain microvascular endothelial cells. In addition, G1 activated the PI3K/ protein kinase B (Akt) and nuclear factor erythroid 2-related factor 2 (Nrf2)/HO-1 pathways both in vivo and in vitro. Furthermore, the administration of LY294002 and ZnPP IX partially reversed the protective effect of G1 on BBB integrity in hemin-stimulated cells. We demonstrated that the activation of GPR30, at least partly through the PI3K/Akt and Nrf2/HO-1 pathways, alleviated BBB damage both in vivo and in vitro. This study introduced a novel therapeutic approach for protecting the BBB after SAH.NEW & NOTEWORTHY The PI3K/Akt and Nrf2/HO-1 pathways might be potential mechanisms by which GPR30 protected the integrity of the BBB in SAH models. Therefore, treatment of SAH with GPR30 activator might be a promising therapeutic strategy.