Effects Of Various Plant Extracts Research Articles

Evaluation of Cyto-Genotoxic Effects of Mucuna Bracteata DC. Ex Kurz Leaf Extracts by Allium Cepa

This study was conducted to evaluate the cyto-genotoxicity of leaf decoctions of Mucuna bracteata by Allium cepa bioassay. This kind of study can be used as a baseline monitoring system for plant products before their use. It is an inexpensive, simple, and reliable test to determine the cytotoxic and genotoxic effects of various plant extracts on living organisms. In this experiment, Allium cepa bulbs were placed in five concentrations (0.5 mg/mL, 2.5 mg/mL, 5.0 mg/mL, 7.5 mg/mL, and 10.0 mg/mL) of leaf decoction, with tap water as the negative control and 20.0 µL/L EMS (Ethylmethene sulphonate) as the positive control. Each concentration had five replicates. The cyto-genotoxicity assay exhibited a dose-dependent influence on the mitotic index. The mitotic index of the negative control and the positive control were 16.8 ± 0.37% and 6.86 ± 1.71%, respectively. The lowest dose of the extract, 0.5 mg/mL, had the highest mitotic index (14.2 ± 0.73%), while the highest dose, 10.0 mg/mL, had the lowest (9.6 ± 0.40%), and all indices were significantly higher than the positive control. Except for the 10 mg/mL concentration, the mitotic indices of other concentrations were significantly lower than the negative control. Different types of chromosome disorders, namely, disorderly prophase, sticky chromosomes, chromosome bridges, laggard chromosomes, pole deviation, vagrant chromosomes, nuclear filaments with terminal expansion, c-mitosis, micronuclei, and elongated nuclei, were observed. Various changes in root size, growth, and color were revealed by macroscopic examinations. Tumors were formed in roots at the highest doses but not at lower concentrations. Therefore, M. bracteata leaf extract showed cytotoxic and genotoxic effects on the Allium root meristem. J. Bio-Sci. 32(2): 71-82, 2024

Inhibitory effects of extracts from Eucalyptus gunnii on α-synuclein amyloid fibrils.

Amyloid fibril formation is associated with various amyloidoses, including neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. Despite the numerous studies on the inhibition of amyloid formation, the prevention and treatment of a majority of amyloid-related disorders are still challenging. In this study, we investigated the effects of various plant extracts on amyloid formation of α-synuclein. We found that the extracts from Eucalyptus gunnii are able to inhibit amyloid formation, and to disaggregate preformed fibrils, in vitro. The extract itself did not lead to cell damage. In the extract, miquelianin, which is a glycosylated form of quercetin and has been detected in the plasma and the brain, was identified and assessed to have a moderate inhibitory activity, compared to the effects of ellagic acid and quercetin, which are strong inhibitors for amyloid formation. The properties of miquelianin provide insights into the mechanisms controlling the assembly of α-synuclein in the brain.

Application of phytochemicals in photodynamic therapy of cancer

SignificanceHerbal medicine is an important part of the health care system in many countries as they exhibit chemopreventive and chemotherapeutic effects. The combination of phytochemicals with Photodynamic therapy is a novel approach to enhancing therapeutic efficacy. ApproachWe studied the effect of various plant extracts, phytochemicals, photosensitizers and their combinations to induce cell death in the lung, breast, colon, cervical and skin cancer cells. The efficacy of the drug moieties was evaluated using viability, proliferation, and cytotoxicity studies. Detailed cell death mechanism was studied using protein expression profiling using immunofluorescence, flow cytometry, ELISA and western blotting. ResultsThe results revealed that PDT and combination therapy groups showed significant cell death in breast and lung cancer cells. Conjugation of photosensitizers with nanoparticles and phytochemicals enhanced the drug delivery and treatment outcome. ConclusionsCombination of PDT with other existing treatments or replacing the synthetic PSs with natural ones is a promising approach in the fight against cancer with improved specific cancer cell targeting.

Allelopathic Potential of Aqueous Plant Extracts Against Seed Germination and Seedling Growth of Weeds

Allelopathy has been described as a negative or positive effect on a specific species of plant caused by a chemical produced by another species of plant. This research analyzed the allelopathic effect of various plant extracts against seed germination and seedling properties of some weed species in two experiments in the autumn of 2021. The impacts of plant extracts, such as peels of pomegranate (Punica granatum), green husk of walnuts (Juglans regia), leaves of eucalyptus (Eucalyptus camaldulensis), galls of Aleppo oak (Quercus infectoria), rhizomes of cogon grass (Imperata cylindrica), and tubers of nutgrass (Cyperus rotundus) plus control on some weed species, like Vaccaria pyramidata, Cynodon dactylon, Sorghum halepense, Trifolium campestre, Portulaca oleracea, Sinapis arvensis, Avena fatua), Hordeum glaucum and Daucus carota were evaluated. The first experiment was conducted in the lab and designed by a factorial-completely randomized design to investigate germination parameters such as final germination percentage (G), mean germination time (MGT), coefficient of the velocity of germination (CVG), and germination rate index (GRI), and another experiment was in a greenhouse and designed in a split-plot pattern, in which the plant extracts were placed in main plots. At the same time, the weed species were distributed in the sub-plots to evaluate the seedling of weeds’ fresh and dry weight and percent of weed inhibition (PWI). The characters’ means (from three replicates) were compared according to Duncan’s multiple range test at the 5% level. The laboratory trial revealed that the green walnut husk was the most successful in reducing all seed germination parameters compared to the control by 13.5, 5.16, and 8.37% for G, MGT, and GRI, respectively. For the greenhouse experiment, the eucalyptus leaves’ extract and pomegranate peels had a higher impact on reducing weed seedlings’ fresh and dry weight and PWI than the control. The most affected species were broad-leaved weeds, while grasses were the least affected. Our results indicated that green walnut husk has a significant role in decreasing seed germination and inhibiting the dry weight of seedlings. Thus, it can be used as a bioherbicide to prevent some weeds from sprouting and growing seedlings.

Effect of various plant extracts on ameliorating lipid peroxidation induced by mercury in Oreochromis mossambicus (Peters 1852)

Effect of various plant extracts on ameliorating lipid peroxidation induced by mercury in Oreochromis mossambicus (Peters 1852)

Effects of various plant extracts on ruminant health for sustainable livestock development

Effects of various plant extracts on ruminant health for sustainable livestock development

Antileishmanial Activity of New Steroidal Saponin Isolated from the Flowers of Allium Austroiranicum

Background & Objective: Plants are reservoirs of bioactive compounds, which are known to be chemically balanced, effective and least injurious as compared to synthetic medicines. The current resistance and the toxic effects of the available drugs have brought the trend to assess the antileishmanial effect of various plant extracts and their purified compound/s. Alliums are rich sources of steroidal saponins, flavonoids, and sulphuric compounds of which steroidal saponins have recently received more attention due to their important pharmacological activities. Allium austroiranicum is a common edible vegetable in western regions of Iran, especially in “Chaharmahal and Bakhtiari” province, where it is named “Lopo” and is considerably used as a raw vegetable, flavoring agent, and as a medicinal plant.
 Materials & Methods: The chloroform-methanolic extract was fractionated using MPLC, and the appropriate fractions were then subjected to isolation and purification of the constituents by HPLC. Structure elucidation was done using comprehensive spectroscopic methods including 1D and 2D NMR. Antileishmanial effects of the isolated compound against the promastigotes of Leishmania major were evaluated using MTT method.
 Results: Phytochemical investigation of chloroform-methanol extract of the plant resulted in the isolation and identification of a Nicotianoside C related steroidal saponin and its chemical structure was determined as (25S)-5α-Spirostan-1β,3βdiol-3-O-{α-L-rhamnopyranosyl-(1→2)-{α-L-rhamnopyranosyl-(1→4)}-[β-D-glucopyranoside}. Investigation of in vitro antileishmanial activity of the isolated compound, in 10 and 50 and 100 μg/mL concentrations, exhibited significant leishmanicidal effects against the promastigotes of Leishmania major.
 Conclusion: The results established a valuable basis for further studies about A. austroiranicum and anti-parasitic activity of steroidal saponins.

In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds

Background and Aims: Adenosine deaminase (EC 3.5.4.4, ADA) is distributed in all human tissues. It catalyses the deamination of adenosine (deoxyadenosine) to inosine (deoxyinosine) via removal of an amino group. The determination of ADAinhibition is of extreme significance in the field of fundamental research and clinical diagnosis. In our study, the effects of various plant extracts and chemicals on ADA activity were investigated. Methods: Adenosine deaminase activity was determined spectrophotometrically at 625 nm. Results: The inhibitory activities of the extracts and chemical compounds increased in a dose-dependent manner. Among the plant extracts used, lemon extract was observed to exhibit the highest ADA inhibition activity with an IC50 value of 0.05 ± 0.001 mg/mL, while quercetin had the highest ADA inhibition (IC50 value of 0.004±0.0005 mg/mL) among the chemical compounds. All plant extracts and chemical compounds showed ADA inhibition activities. Conclusion: The obtained results indicate that plant extracts and some chemical compounds examined in this study can be a potential source of novel ADA-inhibitors for therapeutics.

Efficacy of different plant extracts in the prevention of radiation dermatitis in radiotherapy patients with nasopharyngeal carcinoma: A protocol for a Bayesian network meta-analysis.

Background:Radiation dermatitis is a common complication in patients with nasopharyngeal carcinoma (NPC) when treated with radiotherapy. Plant extracts have good effects on the prevention of radiation dermatitis in patients with NPC when treated with radiotherapy. However, there is insufficient comparison among the currently used plant extracts. Therefore, the purpose of this study was to explore the efficacy of different plant extracts in the prevention of radiation dermatitis in patients with NPC by Bayesian network meta-analysis.Methods:We searched Chinese and English databases to collect all randomized controlled trials (RCT) of plant extracts for the prevention of radiation dermatitis in NPC patients who underwent radiotherapy up to October 2020. Two researchers then independently screened articles, extracted data and evaluated the quality selected literatures. All data were processed by Stata 14.0 and WinBUGS V.1.4.3.Results:We applied Bayesian statistical model for network meta-analysis, ranked the effects of various plant extracts, and adopted GRADE method to explain the results.Conclusion:Our study is expected to provide high-quality evidence-based medicine advice for the prevention of radiation dermatitis in patients suffering from NPC undergoing radiotherapy.Ethics and dissemination:Ethical approval was not required for this study. The systematic review will be published in a peer-reviewed journal, presented at conferences, and will be shared on social media platforms. This review would be disseminated in a peer-reviewed journal or conference presentations.OSF REGISTRATION NUMBER:DOI 10.17605/OSF.IO/6SV45.

Pathophysiology, Clinical Characteristics of Diabetic Cardiomyopathy: Therapeutic Potential of Natural Polyphenols.

Diabetic cardiomyopathy (DCM) is an outcome of disturbances in metabolic activities through oxidative stress, local inflammation, and fibrosis, as well as a prime cause of fatality worldwide. Cardiovascular disorders in diabetic individuals have become a challenge in diagnosis and formulation of treatment prototype. It is necessary to have a better understanding of cellular pathophysiology that reveal the therapeutic targets and prevent the progression of cardiovascular diseases due to hyperglycemia. Critical changes in levels of collagen and integrin have been observed in the extracellular matrix of heart, which was responsible for cardiac remodeling in diabetic patients. This review explored the understanding of the mechanisms of how the phytochemicals provide cardioprotection under diabetes along with the caveats and provide future perspectives on these agents as prototypes for the development of drugs for managing DCM. Thus, here we summarized the effect of various plant extracts and natural polyphenols tested in preclinical and cell culture models of diabetic cardiomyopathy. Further, the potential use of selected polyphenols that improved the therapeutic efficacy against diabetic cardiomyopathy is also illustrated.

Dentin erosion preventive effects of various plant extracts: An in vitro atomic force microscopy, scanning electron microscopy, and nanoindentation study.

The overall consumption of acidic beverages has become more common, making the prevention and treatment of dental erosion an important consideration. The aim of this in vitro study was to evaluate the effect of various plant extracts in preventing dentin erosion. Seven experimental groups (fluoride-free water, fluoride-containing mouthwash [Colgate Plax], green tea, rosehip, clove, pomegranate, and grape seed) were formed, each consisting of 20 bovine dentin samples. The specimens were exposed daily to demineralization and remineralization cycles three times per day over 5 days through a 5-min plant extract application before each erosive episode. Surface roughness, nanohardness values, and morphological changes on dentin surfaces were examined using atomic force microscopy (AFM), nanoindentation, and scanning electron microscopy (SEM). The data were subjected to Kruskal-Wallis and Friedman tests (p < .05). There were statistically significant differences between the groups in terms of nanohardness values (p < .05), except for the pomegranate and grape seed groups. The highest nanohardness value was observed in the clove group (1.24 ± 0.34 GPa), whereas the lowest nanohardness value was noted in the grape seed group (0.20 ± 0.04 GPa). The nanohardness values of positive and negative control groups after erosion cycles were statistically higher than the initial nanohardness values (p < .05).There was no statistically significant difference between the groups in surface roughness values (p > .05). Macromolecular deposits were observed both in the SEM and AFM images of the pomegranate, Colgate, and rosehip groups. It can be concluded that the clove extract group is more successful in preventing dentin erosion than the other groups. Green tea is also effective in preventing dentin erosion, similar to clove extract. The application of plant extracts may be a new treatment strategy in preventing dentin erosion. Plant extracts may also reduce the severity of existing dentin erosion. Clove and green tea extracts may present novel natural therapy potential by inhibiting dentin erosion.

Effect of plant extracts and chemical fungicide on viability and percentage of seed-borne fungal infection on calliandra (Calliandra callothyrsus) seed

Calliandra (Calliandra callothyrsus) is one of the potential tree species that can be used as bio-energy resources. Unfortunately, fungi are one of the factors that can infect and decrease the viability of calliandra seeds. Some efforts are needed to improve the viability and inhibit fungal infection through biological or chemical treatments. The study was conducted to determine the effects of various plant extracts and chemical fungicide on the germination capability, as well as the inhibition of fungal infection in calliandra seeds. Parameters observed in this study were seed germination and fungi infection percentage. Seeds were treated by immersing them in hot water (five minutes) followed by chemical fungicides (i.e., benomyl, mancozeb) or plant extracts (i.e., ginger, onion, garlic, turmeric) for one hour. The study design used was a completely randomized design. The results showed that all the treatments could increase seed viability significantly. The treatment with immersion in onion, ginger and mancozeb was more effective on seed germination ability (88.67%, 8.33%, 86%) and reduced in fungal infections (11.33%, 5.33%, 0%). These treatments resulted in a significant difference between seed germination compared with the hot water and turmeric treatments. The best germination and reduction of fungal infection on calliandra seeds were showed on treatment by onion and mancozeb, respectively. The results revealed that garlic, onion, turmeric have a significant effect in inhibiting all fungal species compared to the hot water treatment. Ginger and onion extracts were effective treatments for increasing seed germination and controlling the fungal infection.

Protective Effect of some plant Extracts on Hyperuricemia in Experimental Animals

ABSTRACT Hyperuricemia (elevated serum levels of uric acid) is a asigin to make gout,and it refer to disease of newel and heart disease and high suger in blood.. Hyperuricemia was induced by oxonic acid (uricase inhibitor) in experimental rats to evaluate the protective effect of alcoholic extracts from parsley shoots, celery seeds and fig leaves. The rats were divided into 6 groups, and the first one served as a normal control group. Three groups of rats were given various plant extracts (celery, parsley and fig) by oral administration using a stomach tube at a dose of 250 mg/kg. A positive control group of rats was administered orally the hypouricemic drug, allopurinol (xanthine oxidase inhibitor) at a dose of 100 mg/kg. A negative control group did not receive any plant extracts or drugs. The various plant extracts and the drug were administered for the rats every day for 9 days. On the 10th day, all groups except the normal control received a single dose of oxonic acid (250 mg/kg) by intraperitoneal injection to induce hyperuricemia. After two hours of hyperuricemia induction by oxonic acid injection, blood samples were collected from all rat groups. The protective effects of various plant extracts were monitored through measurement of uric acid and other blood biochemical analyses for the rats as well as assay of xanthine oxidase enzyme in liver tissues. The data indicated elevation of uric acid, urea, creatinine and potassium, and lower in the amount of total calcium in serum of hyperuricemic rats (negative control) compared to the normal control group. These results indicated that oxonic acid caused hyperuricemia and renal dysfunction in the negative control group. The protective effects of various plant extracts were established by appearance the levels of uric acid and other kidney function tests near to their normal values which appeared in the normal control group. The different plant extracts exhibited protective effects against hyperuricemia in variant efficacies compared to allopurinol. These efficacies were in the following order: fig > allopurinol > celery ≈ parsley. Comparatively, the different plant extracts exhibited inhibitory effects on the activity of liver xanthine oxidase enzyme in variant efficacies compared to allopurinol. These efficacies were in the following order: allopurinol > fig > celery ≈ parsley. It can be noticed that fig extract was the most effective treatment against hyperuricemia while allopurinol was the strongest inhibitor against xanthine oxidase activity. This may be due to that fig extract possessed a dual action as hypouricemic agent (inhibition of xanthine oxidase which led to decreasing of uric acid synthesis in liver, and inhibition of uric acid reabsorption in kidney which led to increasing of uric acid excretion in urine) while allopurinol possessed the first action only as hypouricemic agent. The hypouricemic activity of fig extract may be ascribed to the presence of flavonoids such as morin while the hypouricemic activity of parsley and celery is related to flavonoids namely apigenin, luteolin, quercetin, myricetin and kaempferol.

Toxic and in vitro anti-acetylcholinesterase and anti-carboxylesterase effects of various plant extracts on Aphis gossypii Glover, 1877 (Hemiptera: Aphididae)

The significance of discovering new active substances that are environment friendly when compared to pesticides, agriculturally sustainable, plant-based, and in the status of GRAS (generally regarded as safe) has been increasing every day. For this purpose, leaves of Daphne odora L., Dieffenbachia amoena L., Eucalyptus camaldulensis L. , Ficus carica L. , Lantana camara L. , Matricaria chamomilla L., Mentha pulegium L. and Nerium oleander L. were collected from Adana in 2018. Toxic and in vitro anti-acetylcholinesterase (AChE) and anti-carboxylesterase (CE) activities of aqueous leaf extracts of these species on the important polyphagous pest, Aphis gossypii Glover, 1877 (Hemiptera: Aphididae), were determined after 24 and 72 h. The fastest and greatest toxic effect was obtained with 20% F. carica extract giving 75.6% mortality. This was followed by N. oleander with 71.6% and D. odora with 62.1% mortality. Aphis gossypii in vitro anti-AcHe and anti-CE activities were highest at 10% concentration of the plant extracts and inhibition levels were 51.8-82.5% with F. carica extract, 40.9-54.9% with D. odora extract and 40.2-82.5% with E. camaldulensis extract. In conclusion, D. odora, E. camaldulensis, F. carica and N. oleander extracts gave promising results for future studies on the discovery of potential xenobiotics against A. gossypii and for pest control.

Toxic Effects of various Plant Extracts against the Rice Leaf Folder (Cnaphalocrosis medinalis Guenee) Pest

Toxic Effects of various Plant Extracts against the Rice Leaf Folder (Cnaphalocrosis medinalis Guenee) Pest

Cytotoxic activities of Telectadium dongnaiense and its constituents by inhibition of the Wnt/β-catenin signaling pathway

BackgroundWnt/β-catenin signaling pathway is a potential target for the treatment of human colon cancer. Thus, the inhibitory effects of various plant extracts on cell proliferation and Wnt signal transduction were evaluated to discover a Wnt signaling inhibitor. PurposeThe present study aimed to investigate the cytotoxicity involved in Wnt pathway of the MeOH extract from Telectadium dongnaiense bark (TDB) and to identify its bioactive constituents by bioassay-guided fractionation. MethodsThe sulforhodamine B-based proliferation assay and the β-catenin/TCF-responsive reporter gene assay were employed as screening systems. The isolation and identification of compounds were elucidated on the basis of spectroscopic methods. Inhibitory effects on the expression levels of Wnt target genes were determined by real-time PCR and western blotting. ResultsThe extract of TDB most strongly inhibited cell proliferation and TOPflash activity (IC50 = 1.5 and 2.0 µg/ml), which was correlated with its inhibitory effects on the expression of Wnt target genes. Three major compounds were isolated from bioactive fractions and were identified as 1,4-dicaffeoylquinic acid (1), quercetin 3-rutinoside (2), and periplocin (3). Only compound 3 showed anti-proliferative activity (IC50 = 0.06 µM) and exhibited Wnt signaling inhibitory effects in HCT116 colon cancer cells. ConclusionsThis study contributes to understanding the cytotoxic properties of TDB extract and its constituents and provides a potent strategy for its further application.

Comparative HPLC-DAD and UHPLC-ESI(-)-HRMS & MS/MS profiling of Hypericum species and correlation with necrotic cell-death activity in human leukemic cells

In the search for potential new anti-cancer agents, the possible anti-proliferative effect of various plant extracts from an in-house extract library was investigated in vitro. The cytotoxic, cytostatic and the pro-apoptotic capacity of the extracts was estimated on the HL-60 human promyelocytic leukemia cell line by the trypan-blue exclusion method, the MTT assay and flow-cytometric analysis. Interestingly, out of all extracts screened, three methanol extracts, namely Hypericum perforatum, Hypericum triquetrifolium and Hypericum rumeliacum, exhibited the most significant activity by promoting necrotic cell-death, accompanied by alterations in the mRNA expression levels of the BCL2 and BAX genes. All the three bioactive extracts were subjected to HPLC-DAD and UHPLC-ESI(−)-HRMS & MS/MS dereplication procedures for correlation purposes. Interesting comparative results related to known constituents such as naphodianthrones, phloroglucinol derivatives and flavonoids were elucidated. To our knowledge, this is the first time that H. triquetrifolium and H. rumeliacum are investigated for both their anti-proliferative properties and their detailed composition.

Plants as Antileishmanial Agents: Current Scenario.

Leishmaniasis is a clinical manifestation caused by the parasites of the genus Leishmania. Plants are reservoirs of bioactive compounds, which are known to be chemically balanced, effective and least injurious as compared with synthetic medicines. The current resistance and the toxic effects of the available drugs have brought the trend to assess the antileishmanial effect of various plant extracts and their purified compound/s, which are summarized in this review. Moreover, it also highlights various traditional remedies used by local healers against leishmaniasis. A systematic cross-sectional study for antileishmanial activity of natural products was carried out using multiple literature databases. The records retrieved since 2000 till year 2016 were analysed and summarized in the form of comprehensive tables and graphs. Natural products are potential source of new and selective agents that can significantly contribute to primary healthcare and probably are promising substitutes of chemicals for the treatment of protozoan diseases like leishmaniasis. Where the researchers prefer to use alcoholic solvents for the extraction of antileishmanial agents from plants, most of the studies are limited to in vitro conditions majorly on using promastigote forms of Leishmania. Thus, there is a need to carry out such activities in vivo and in host macrophages. Further, there is a need of mechanistic studies that can help taking few of the promising pure compounds to clinical level. Copyright © 2016 John Wiley & Sons, Ltd.

Identifying Combinatorial Growth Inhibitory Effects of Various Plant Extracts on Leukemia Cells through Systematic Experimental Design

Plant extracts are widely studied for their anti-cancer and cancer preventive effects. In this study, we compared the leukemia growth inhibition effects of seven different plant extracts, theaflavin, epigallocatechin gallate (EGCG), epicathechin (EC), apigenin, quercetin, chrysin and tannic acid, in vitro using the K562 erythroleukemia cell line and application of the design of experiments (DoE) methodology. Our systematic approach enabled us to isolate the main factor contribution, two-factor interactions and produced interaction relationships and/or models to describe growth inhibitory effects of different plant extracts when they are used in combination. The results identified tannic acid as the most significant inhibitor in this group and had synergistic effects with EGCG at specific concentrations. The fitted model of their combined effects showed that the most potent combination is at low concentrations of tannic acid (10 - 20 μM) and high concentrations of EGCG (80 - 100 μM). We further showed that tannic acid induced both growth inhibition and apoptosis in K562 cells in ranges between 10 - 100 μM. The polyphenol caused cell cycle arrest at G2- phase under the higher concentrations. In summary, use of DoE techniques effectively identified the most prominent inducer in this group of plant bioactive compounds and produced combinatorial bioactivity of various polyphenols and flavonoids over the entire range of concentrations under study. This study exemplifies the usefulness of DoE and serves as a guide in its utility for in vitro assessment of bioactivity in plant constituents.

Effect of Various Plant Extracts on Dry Root Rot of Chillies Caused by Sclerotium rolfsii

Eight different plant extracts were evaluated in vitro against Sclerotium rolfsii causing dry root rot in chillies. Among these, leaf extract of neem (Azadirachta indica) caused maximum inhibition of mycelial growth (80.74%), followed by periwinkle Vinca rosea (78.8%) and bottlebrush (Callistemon, 74.8%) respectively. Sclerotial production was inhibited to an extent of 11% and the inhibition caused was maximum with neem extract, followed by Polyalthia longifolia and V. rosea extracts. Though sclerotial germination was inhibited by 30% to 95% in various treatments, the most effective treatment was that of neem leaf extract (95%), followed by ginger extract (92%).