Abstract
Plant extracts are widely studied for their anti-cancer and cancer preventive effects. In this study, we compared the leukemia growth inhibition effects of seven different plant extracts, theaflavin, epigallocatechin gallate (EGCG), epicathechin (EC), apigenin, quercetin, chrysin and tannic acid, in vitro using the K562 erythroleukemia cell line and application of the design of experiments (DoE) methodology. Our systematic approach enabled us to isolate the main factor contribution, two-factor interactions and produced interaction relationships and/or models to describe growth inhibitory effects of different plant extracts when they are used in combination. The results identified tannic acid as the most significant inhibitor in this group and had synergistic effects with EGCG at specific concentrations. The fitted model of their combined effects showed that the most potent combination is at low concentrations of tannic acid (10 - 20 μM) and high concentrations of EGCG (80 - 100 μM). We further showed that tannic acid induced both growth inhibition and apoptosis in K562 cells in ranges between 10 - 100 μM. The polyphenol caused cell cycle arrest at G2- phase under the higher concentrations. In summary, use of DoE techniques effectively identified the most prominent inducer in this group of plant bioactive compounds and produced combinatorial bioactivity of various polyphenols and flavonoids over the entire range of concentrations under study. This study exemplifies the usefulness of DoE and serves as a guide in its utility for in vitro assessment of bioactivity in plant constituents.
Highlights
Natural plant extracts with anti-cancer properties are generally classified as strong anti-oxidants widely known as polyphenols
We further showed that tannic acid induced both growth inhibition and apoptosis in K562 cells in ranges between 10 - 100 μM
In this design of experiments (DoE) design, tannic acid is confounded with AF, BE and CD, quercetin is confounded with AC, BG and DF, and chrysin is confounded with AG, BC and DE
Summary
Natural plant extracts with anti-cancer properties are generally classified as strong anti-oxidants widely known as polyphenols. Flavones found in plants and vegetables such as apigenin and quercetinare known to induce apoptosis in leukemia cells [4]. Other plants extracts that were previously studied as anti-cancer agents include chrysin which is a naturally occurring flavone commonly found in blue passion flower, and tannic acid which is widely found in food plants. The goal of this study is to demonstrate a highly effective and efficient method to perform quantitative comparisons between individual contributions of different plant extractson leukemia cells and identify synergistic effects between two components. This was achieved by using a systematic experimental design approach
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
