Drought Events Research Articles

Past, present and future changes in the annual streamflow of the Lancang-Mekong River and their driving mechanisms

The rapid development of the Greater Mekong Subregion (GMS) makes it essential to understand the major mechanisms controlling the streamflow, especially for the Lancang-Mekong River (abbr. Mekong River). We used instrumental annual streamflow data (1960–2007) from Chiang Saen hydrological station and several gridded hydroclimatic datasets to explore the hydroclimatic evolution of the Mekong River, together with its driving mechanisms. We found that changes in the Mekong streamflow are consistent with precipitation changes, and the Mekong is thus a precipitation-dominated river that is susceptible to the effects of ongoing climate change. The instrumental record of Mekong annual streamflow is closely related to hydroclimatic changes, especially those related to moisture, within the area from the Hengduan Mountains to the Chiang Saen Station. Based on these gridded records, we extended the Mekong annual streamflow record to cover 1891–2021 using nested multiple linear regression fitting. The fitted streamflow explained up to 57.6 % of the instrumental changes and it indicates that the major 2019 drought was not unique over the past century. Despite extremely low precipitation and high temperatures, it is likely that the effects of drought can be mitigated via hydraulic engineering regulation. Climatological analyses showed that the Mekong annual streamflow is driven by the El Niño-Southern Oscillation (ENSO) and the Indian Ocean Dipole (IOD), which is consistent with observed quasi-interannual cycles of 3–4 years. A multi-model ensemble of CMIP6 revealed that the Mekong annual streamflow will experience an upward trend in the future, accompanied by the more extreme impacts of ENSO and the IOD.

The Effect of Drought on Agronomic and Plant Physiological Characteristics of Cocksfoot (Dactylis glomerata L.) Cultivars

The Effect of Drought on Agronomic and Plant Physiological Characteristics of Cocksfoot (Dactylis glomerata L.) Cultivars

Crop Diversification and Fertilization Strategies in a Rainfed System with Drought Periods

Crop diversification and the reduction of nitrogen (N) inputs are key issues in the EU for more sustainable agriculture. An experiment was set up in a semiarid rainfed Mediterranean system. Our hypothesis was that these challenges could be addressed by introducing new crops and using pig slurries (PSs). The experimental factors were N fertilization at sowing (with or without PS) combined (according to a split-block design) with N fertilization as topdressing (the control, two N mineral rates, and two N rates from PS). Barley, rapeseed, and pea performances were evaluated in two different crop sequences: (i) barley–rapeseed or rapeseed–barley after a fallow season, and (ii) barley–pea or pea–barley after a fallow season followed by a non-fertilized barley crop. The results of the four-year study demonstrated that under a spring drought risk, barley performed better than peas in terms of relative crop yield maintenance. After fallow, N can be saved while maintaining the yields and total biomass of barley and rapeseed. In the second crop sequence, maximum pea and barley yields were associated with a minimum topdressing of 60 or 120 kg mineral N ha−1, respectively. However, slurry fertilization at sowing also allowed the highest yields for barley. Rapeseed and peas can be introduced to reduce N fertilization inputs. However, the obtained yield plateau for pea and rapeseed (3 and 4 Mg ha−1, respectively) and the effect of a yield spring drought on pea yields (50% reduction) might be a constraint for the success of EU policies on crop diversification.

Trends and Directions in Oats Research under Drought and Salt Stresses: A Bibliometric Analysis (1993-2023).

With global climate change leading to increasing intensity and frequency of droughts, as well as the growing problem of soil salinization, these factors significantly affect crop growth, yield, and resilience to adversity. Oats are a cereal widely grown in temperate regions and are rich in nutritive value; however, the scientific literature on the response of oat to drought and salt stress has not yet been analyzed in detail. This study comprehensively analyzed the response of oat to drought stress and salt stress using data from the Web of Science core database and bibliometric methods with R (version4.3.1), VOSviewer (version 1.6.19), and Citespace (version6.3.1.0) software. The number of publications shows an increasing trend in drought stress and salt stress in oat over the past 30 years. In the field of drought-stress research, China, the United States, and Canada lead in terms of literature publication, with the most academic achievements being from China Agricultural University and Canadian Agricultural Food University. The journal with the highest number of published papers is Field Crops Research. Oat research primarily focuses on growth, yield, physiological and biochemical responses, and strategies for improving drought resistance. Screening of drought-tolerant genotypes and transformation of drought-tolerant genes may be key directions for future oat drought research. In the field of salt-stress research, contributions from China, the United States, and India stand out, with the Chinese Academy of Agricultural Sciences and Inner Mongolia Agricultural University producing the most significant research results. The largest number of published articles has been found in the Physiologia Plantarum journal. Current oat salt-stress research primarily covers growth, physiological and biochemical responses, and salt-tolerance mechanisms. It is expected that future oat salt research will focus more on physiological and biochemical responses, as well as gene-editing techniques. Despite achievements under single-stress conditions, combined drought and salt-stress effects on oat remain understudied, necessitating future research on their interaction at various biological levels. The purpose of this study is to provide potential theoretical directions for oat research on drought and salt stress.

Photosynthesis, Anatomy, and Metabolism as a Tool for Assessing Physiological Modulation in Five Native Species of the Brazilian Atlantic Forest.

The Brazilian Atlantic Forest, renowned for its exceptional species richness and high endemism, acts as a vital reservoir of terrestrial biodiversity, often referred to as a biodiversity hotspot. Consequently, there is an urgent need to restore this forest to safeguard certain species and to unravel the ecophysiological adaptations of others. This study aims to integrate some physiological parameters, including gas exchange and chlorophyll a fluorescence, with anatomical and metabolic techniques to elucidate how five different native species (Paubrasilia echinata, Chorisia glaziovii, Clusia nemorosa, Licania tomentosa, and Schinus terebinthifolius), each occupying distinct ecological niches, respond to seasonal variations in rainfall and their consequences. Our investigation has revealed that C. nemorosa and P. echinata exhibit robust mechanisms to mitigate the adverse effects of drought. In contrast, others demonstrate greater adaptability (e.g., S. terebinthifolia and C. glaziovii). In this context, exploring metabolic pathways has proven invaluable in comprehending the physiological strategies and their significance in species acclimatization. This study provides a comprehensive overview of the impact of water restrictions and their consequential effects on various species, defining the strategies each species uses to mitigate water privation during the dry season.

The effects of drought on biodiversity in UK river ecosystems: Drying rivers in a wet country

AbstractClimate change is interacting with water resource pressures to alter the frequency, severity and spatial extent of drought, which can thus no longer be considered a purely natural hazard. Although particularly severe ecological impacts of drought have occurred in drylands, its effects on temperate ecosystems, including rivers, are also considerable. Extensive research spanning a diverse range of UK rivers offers an opportunity to place the effects of past drought in the context of intensifying climate change and to examine the likely effects of future drought in a typically cool, wet country. Here, drought manifests instream as deficits in surface water, modified flow velocities, and—increasingly—partial or complete drying of previously perennial and naturally non‐perennial reaches. As a result, drought causes declines in the taxonomic and functional biodiversity of freshwater communities including microorganisms, algae, plants, invertebrates and fish, altering ecological processes and associated benefits to people. Although freshwater communities have typically recovered quickly after previous UK droughts, an increase in drought extremity may compromise recovery following future events. The risk of droughts that push ecosystems beyond thresholds to persistent, species‐poor, functionally simplified states is increasing. Research and monitoring are needed to enable timely identification of rivers approaching such thresholds and thus to inform interventions that pull these ecosystems back from the brink. Management actions that support natural flow regimes and promote natural processes that diversify instream habitats, including drought refuges, are also crucial to support biodiversity within functional river ecosystems as they adapt to a changing world.This article is categorized under: Water and Life > Nature of Freshwater Ecosystems Water and Life > Stresses and Pressures on Ecosystems Water and Life > Conservation, Management, and Awareness

Desert plants, arbuscular mycorrhizal fungi and associated bacteria: Exploring the diversity and role of symbiosis under drought.

Desert plants, such as Agave tequilana, A. salmiana and Myrtillocactus geometrizans, can survive harsh environmental conditions partly due to their symbiotic relationships with microorganisms, including arbuscular mycorrhizal fungi (AMF). Interestingly, some of these fungi also harbour endosymbiotic bacteria. Our research focused on investigating the diversity of these AMFs and their associated bacteria in these plants growing in arid soil. We found that agaves have a threefold higher AMF colonization than M. geometrizans. Metabarcoding techniques revealed that the composition of AMF communities was primarily influenced by the plant host, while the bacterial communities were more affected by the specific plant compartment or niche they inhabited. We identified both known and novel endofungal bacterial taxa, including Burkholderiales, and confirmed their presence within AMF spores using multiphoton microscopy. Our study also explored the effects of drought on the symbiosis between A. tequilana and AMF. We discovered that the severity of drought conditions could modulate the strength of this symbiosis and its outcomes for the plant holobiont. Severe drought conditions prevented the formation of this symbiosis, while moderate drought conditions promoted it, thereby conferring drought tolerance in A. tequilana. This research sheds light on the diversity of AMF and associated bacteria in Crassulacean Acid Metabolism (CAM) plants and underscores the crucial role of drought as a factor modulating the symbiosis between A. tequilana and AMF. Further research is needed to understand the role of endofungal bacteria in this response.

Response of changes in lake area to drought and land use change

The lake area is a crucial parameter that characterizes the state of a lake. Under the dual pressures of climate change and human activity, the magnitude and frequency of changes in lake areas become more pronounced. This process poses a serious threat to the local ecological environment. In this study, we constructed a lake water extraction model (LakeNet) based on a fully convolutional neural network. We extracted and analyzed the spatiotemporal characteristics of the area of nine major lakes from 1987 to 2022, as well as the driving factors behind these changes. Our results indicate that: 1) LakeNet exhibits high extraction accuracy and can remove some clouds. 2) The area of the nine major lakes shows a fluctuating downward trend (−8.11km2/10a), with drought and land use changes identified as significant driving forces behind the changes in lake boundaries, drought events caused the lake area to decrease, and the expansion of cropland further reduced the lake area. 3) Due to variations in lake area, the impact of drought on the area of the nine major lakes exhibits a lag effect, smaller lakes are likely to respond more quickly to drought.

Comparative analysis of SPI, SPEI, and RDI indices for assessing spatio-temporal variation of drought in Türkiye

AbstractThis research presents a comprehensive drought analysis using climate data obtained from 219 homogeneously distributed meteorological stations in Türkiye between 1991 and 2022. In this context, Standard Precipitation Index (SPI), Standardized Precipitation Evapotranspiration Index (SPEI) and Reconnaissance Drought Index (RDI) drought indices were used and comparative analysis was made. Türkiye. The study demonstrates that below-normal precipitation over extended periods and increasing temperatures have contributed to the increased frequency of meteorological drought events. Türkiye's topographic conditions, particularly its location in the Mediterranean basin, significantly influence drought occurrences. It is noted that over the past 20 years, Türkiye has been trending towards drier conditions, with rising temperatures reinforcing this trend. The study observes that the moderate drought class range is the most frequently recurring in the SPI, SPEI, and RDI methods utilized. Regarding atmospheric conditions affecting the climate in Türkiye, it is observed that increased drought severity stands out prominently in years when the North Atlantic Oscillation is positive. During these years, increased drought severity is evident in the SPI, SPEI, and RDI indices, particularly in winter and autumn, while a wide area experiences drought effects in the summer months. Long-term analyses emphasize that drought periods occur less frequently but have more prolonged impacts, attributed to variations in precipitation patterns from year to year and the influence of rising temperatures due to global climate change. The potential future increase in drought in the Mediterranean basin due to global climate change and Türkiye's vulnerability to this situation could have adverse effects on water resources, food security, energy sources, and ecosystems.

Analysis of Flash Drought and Its Impact on Forest Normalized Difference Vegetation Index (NDVI) in Northeast China from 2000 to 2020

Flash drought is characterized by rapid onset and short-duration drought conditions caused by a combination of factors, including high evaporation, high temperature, and prolonged periods of little to no precipitation, leading to a sudden and severe decrease in soil moisture levels. In comparison to conventional drought, it is more susceptible to the effects of global warming and has the potential to become a common drought phenomenon in the coming years, necessitating further research. In this paper, we focused on flash drought events, specifically in forest parts of northeastern China that are included within the Greater Khingan Mountains (GKM), Lesser Khingan Mountains (LKM), and Changbai Mountains (CM), using daily soil moisture data as well as SPOT- VEGETATION NDVI satellite data from 2000 to 2020 and determined their impact on the forest NDVI. Our major findings are as follows. (1) The forest within GKM had the maximum area being affected by flash drought events. (2) The frequency ranged from 1 to 2 times, whereas the total duration varied between 20 and 55 days over the study area in a 21-year period. (3) Flash drought was most common in the plant-growing seasons. (4) The flash drought events had a negative influence on the forest NDVI. Our study contributes to a deeper understanding of the flash drought dynamics in forest areas of northeast China for flash drought monitoring, prediction, and management strategies in this region.

Ecological stress memory in wood architecture of two Neotropical hickory species from central-eastern Mexico

BackgroundDrought periods are major evolutionary triggers of wood anatomical adaptive variation in Lower Tropical Montane Cloud Forests tree species. We tested the influence of historical drought events on the effects of ecological stress memory on latewood width and xylem vessel traits in two relict hickory species (Carya palmeri and Carya myristiciformis) from central-eastern Mexico. We hypothesized that latewood width would decrease during historical drought years, establishing correlations between growth and water stress conditions, and that moisture deficit during past tree growth between successive drought events, would impact on wood anatomical features. We analyzed latewood anatomical traits that developed during historical drought and pre- and post-drought years in both species.ResultsWe found that repeated periods of hydric stress left climatic signatures for annual latewood growth and xylem vessel traits that are essential for hydric adaptation in tropical montane hickory species.ConclusionsOur results demonstrate the existence of cause‒effect relationships in wood anatomical architecture and highlight the ecological stress memory linked with historical drought events. Thus, combined time-series analysis of latewood width and xylem vessel traits is a powerful tool for understanding the ecological behavior of hickory species.

Differences in drought characteristics, progression, and recession across ecosystem types in the pantropical region of the Lancang-Mekong River Basin

Exploring the development and impacts of drought across different ecosystems can offer new insights for mitigating the adverse effects of drought events. Using the pantropical Lancang-Mekong River Basin as the study region, we investigated the agricultural, ecological, and hydrological drought characteristics and explored their drought progression and recession rates across four vegetation ecosystem types: tropical forests, subtropical forests, shrubs, and crops. We utilized newly developed drought indices based on the ERA5-Land reanalysis dataset, GOSIF chlorophyll fluorescence data, and modified Moderate Resolution Imaging Spectroradiometer (MODIS) land cover data. The results showed that agricultural and hydrological droughts exhibited increasing trends from 2001 to 2021, whereas ecological drought displayed a decreasing trend over the same period. The cropland region experienced the fewest drought events, shortest drought durations, slowest progression rates, and lowest recession rates. By contrast, the two evergreen, broadleaf forest ecosystems (subtropical and tropical forests) experienced the highest number of drought events and fastest progression and recession rates. The findings suggest a trade-off relationship between vegetation resistance and recovery, where faster drought onset is associated with faster drought recession for ecological drought. Given the more severe challenges posed by agricultural and hydrological droughts, the riparian countries in the Lancang-Mekong River Basin should adopt proactive financial and management measures to mitigate the adverse impacts of these drought types. The insights gained from this study can inform the development of targeted strategies for drought monitoring, preparedness, and response across diverse ecosystems.

Climate change in Tigray, Ethiopia: Assessing impacts on smallholders’ risk perception and food security

Amidst the ongoing global climate change crisis, characterized by unpredictable weather patterns and increasing environmental shocks, farming communities are among the most vulnerable. This vulnerability is particularly evident in Tigray, Ethiopia. This study, which investigates the incidence of climate change and its variability in relation to risk perception and food security of farming communities in Tigray, has significant implications for future action. It uses climate and the Living Standards Measurement Study panel data. A multi-method analytical procedure is employed to assess changes in temperature and rainfall, understand farmers’ climate change risk perception, and signify the welfare effects of droughts on farmers. The study finds significant warming trends, particularly increasing warming nights, and variable but increasing rainfall trends. Farmers do perceive climate change as a reality (85.53%) and a threat to their welfare (23%). Cooperation, diversification, irrigation, wealth, drought, and access to services were identified as drivers of this risk perception. Similarly, the study found drought is a significant driver of food insecurity. Other variables associated with food security status substantially are wealth, head marital status, and non-farm participation. It concludes that climate change is a reality and a perceived risk in Tigray. It poses a threat to the lives and livelihoods of its farming communities. It is recommended that farm households’ adaptive capacity be enhanced through improved livelihood options, promotion of cooperation, development of irrigation schemes, and increased access to weather and climate information.

Interactive effects of mycorrhizal, Azospirillum and nitrogen+phosphorus with limited irrigation on yield and morpho-physiological traits of evening primrose (Oenothera biennis L.) in arid and semi-arid regions

Evening primrose (Oenothera biennis L.) is a valuable medicinal plant known for its oil, which is rich in gamma-linolenic acid. However, the productivity and quality of evening primrose can be significantly affected by drought stress, a common challenge in arid and semi-arid regions. Water deficit stress not only limits plant growth and development but also affects physiological and biochemical processes. To mitigate the adverse effects of drought, the use of chemical and biological fertilizers has been explored. Mycorrhizal fungi and Azospirillum are known for their ability to enhance plant resilience to abiotic stresses, including drought, by improving nutrient uptake and promoting plant growth. Nitrogen and phosphorus (N+P) are essential nutrients that play crucial roles in plant metabolism and stress tolerance. This study aimed to assess the impact of mycorrhizal fungi, Azospirillum, and N+P on evening primrose under drought conditions. One experiment was conducted in two locations, Tehran (semi-arid) and Varamin (arid) in 2014 and 2015, using a greenhouse for growing plants and through field transplantation. Using a split factorial design within a randomized complete block layout, three irrigation regimes (50 %, 40 %, and 30 % of field capacity) constituted the main plots, while the subplots included three chemical fertilizer treatments (none, 50 %, and 100 % of N+P) and four biological fertilizer treatments (non-inoculated and inoculated with mycorrhizal fungi and Azospirillum) in factorial combinations. The study revealed that drought, both moderate and severe, resulted in reduced plant height, shoot dry weight, leaf area index, seed yield, harvest index, phosphorus content, relative water content, and oil yield. Notably, the highest water use efficiency occurred under moderate water deficit stress. Severe water deficit stress, however, led to the highest root dry weight, root ratio to shoot dry weight, and catalase activity. The application of chemical and biological fertilizers alleviated the negative effects of water deficit stress on plant growth and yield. Evening primrose water use efficiency increased under moderate water deficit stress, particularly when coupled with the application of chemical (N+P) and biological (mycorrhizal fungi and Azospirillum) fertilizers. The study emphasized the positive impact of mycorrhizal and Azospirillum inoculation on enhancing evening primrose growth under water deficit stress.

Oasis civilization collapse under 3.9 ka climate event in Bactria, Central Asia

Central Asia played a significant role in the early exchange of civilizations across Eurasia. The arid climate, which makes the local ecology sensitive to climate change and the well-preserved archaeological remains, make Central Asia an ideal location for studying the mechanisms of interactions between civilization evolution and environmental change. This research presents archaeobotanical, palynological and stable isotope records from the Djarkutan site in southeastern Uzbekistan, which was occupied between 4100 and 3700 cal yr BP. Our research shows that in the Late Bronze Age, after 4000 yr BP, the local agricultural structure was highly complex. Pollen and stable isotope result indicate a sudden drought event occurred in the local area around 3900 yr BP, which had an impact on the local oasis agricultural system. Subsequently, this event promoted the migration of northern steppe populations into Central Asia, leading to the development of an agro-pastoral economy in the research area.

Projection of Changes in Rainfall and Drought Based on CMIP6 Scenarios on the Ca River Basin, Vietnam

In this study, future precipitation and drought in the Ca river basin, Vietnam, were projected based on an ensemble of 27 CMIP6 models for four climate change scenarios. The impact of climate change on precipitation and drought was investigated. Monthly precipitation observation data were adjusted using the bias correction method. To detect drought events, the Standard Precipitation Index (SPI) was employed. Changes in drought were assessed using SPI3, SPI6, and SPI12. Although the amount of annual total precipitation slightly increased, the drought events may become more severe. There is a high likelihood of increased drought intensity and severity in Vietnam due to climate change. The frequency of droughts is likely to change depending on the location and climate change scenario. We found that the frequency and severity of droughts may be altered depending on the window size of SPI. The short-term drought events will be more frequent and severe, and long-term drought events will become more severe in the Ca river basin.

Drought and nitrogen deposition regulate plant nutrient resorption in a typical steppe

The impending rise in drought events in grasslands of northern China over the next few decades, coupled with escalating nitrogen (N) deposition, will have an important impact on nutrient resorption. Previous research has mostly focused on examining the individual effect of specific drought scenario or N enrichment on nutrient resorption. Nonetheless, the impacts of different drought regimes on the resorption of nutrients have rarely been distinguished, especially under the scenario of N enrichment in temperate typical steppe in Inner Mongolia, we studied how intense drought (excluding 100 % rainfall in June), chronic drought (excluding 50 % rainfall during June-August), reduced rainfall frequency (reducing half rainfall events without changing rainfall amount in June-August), and N deposition (0 and 10 g N m−2 yr−1) affected the efficiency of plants in reabsorbing N and phosphorus (P). Both intense and chronic drought significantly reduced N (by 9.41 % and 9.53 %, respectively) and P resorption efficiency (by 6.71 % and 6.62 %, respectively) in plant communities (defined as the proportion of N and P nutrient resorption from senescing leaves), however reducing rainfall frequency had less effects on plant community N and P resorption efficiency. Nitrogen deposition had no effects on N and P resorption efficiency. Drought and N addition interacted to affect plant community P resorption efficiency. Structural equation modeling (SEM) showed that drought reduced N and P resorption efficiencies in plant communities by directly decreasing soil moisture, suppressing nutrient concentrations in green leaves, and enhancing soil nutrient content. Nitrogen deposition reduced P resorption efficiency by reducing P concentration in green leaves, but this effect was offset by the reduction of soil P availability. These results imply that rainfall amount is more important than rainfall frequency in determining the nutrient resorption efficiency of plant communities in the typical steppe. This study highlights the importance of soil water and N availabilities as well as nutrient concentrations in green leaves in modulating the responses of plant nutrient resorption to global change in the typical steppe.

The physiochemical characteristics and glycerolipid profile of Cycas panzhihuaensis in response to individual and combined drought and freezing temperature stress

The frequency and intensity of the occurrence of drought (D) events during winter are increasing in most areas of China. To explore the interactive effects of D and freezing temperature (F) on plants of endangered Cycas panzhihuaensis, some physiochemical characteristics and the lipid profile were determined. Drought and F stress had no or little impact on the traits of leaves, which, however, bleached following a combination of D and F treatment (DF). Drought treatment did not affect the chlorophyll fluorescence parameters and the flavonoid content of C. panzhihuaensis. Besides the increase in flavonoid content, a decrease of photochemical efficiency and an increase of heat dissipation were induced by both F and DF treatment, with the effects being greater in the latter treatment. The malondialdehyde content decreased significantly and the total antioxidant capacity increased significantly in the plants exposed to both D and DF treatments. The D treatment did not impact the amount of phospholipids but led to an accumulation of saccharolipids. Additionally, the amount of both phospholipids and saccharolipids remained unchanged following F treatment but decreased significantly following DF treatment compared with those of the control. The unsaturation level did not change significantly in most lipid classes of membrane glycerolipids following various stresses but increased significantly in phosphatidylserine, monogalactosylmonoacylglycerol, digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerol following D or both D and F treatments. Generally, plants of C. panzhihuaensis showed relatively strong tolerance to individual D stress, while D aggravated the F-induced damage, which was likely caused by the degradation of the membrane glycerolipids.

Enhancing drought stress tolerance and growth promotion in chiltepin pepper (Capsicum annuum var. glabriusculum) through native Bacillus spp.

The drought can cause a decrease in food production and loss of biodiversity. In northern Mexico, an arid region, the chiltepin grows as a semi-domesticated crop that has been affected in its productivity and yield. An alternative to mitigate the effect of drought and aid in its conservation could be using Plant Growth-Promoting Bacteria (PGPB). The present study evaluated the capacity of native Bacillus spp., isolated from arid soils, as PGPBs and drought stress tolerance inducers in chiltepin under controlled conditions. Chiltepin seeds and seedlings were inoculated with native strains of Bacillus spp. isolated from arid soils, evaluating germination, vegetative, and drought stress tolerance parameters. The PGPBs improved vegetative parameters such as height, stem diameter, root length, and slenderness index in vitro. B. cereus (Bc25-7) improved in vitro survival of stressed seedlings by 68% at −1.02 MPa. Under greenhouse conditions, seedlings treated with PGPBs exhibited increases in root length (9.6%), stem diameter (13.68%), leaf fresh weight (69.87%), and chlorophyll content (38.15%). Bc25-7 alleviated severe water stress symptoms (7 days of water retention stress), and isolates B. thuringiensis (Bt24-4) and B. cereus (Bc25-7, and Bc30-2) increased Relative Water Content (RWC) by 51%. Additionally, the treated seeds showed improved germination parameters with a 46.42% increase in Germination Rate (GR). These findings suggest that using PGPBs could be an alternative to mitigate the effect of drought on chiltepin.

Drought stimulus enhanced stress tolerance in winter wheat (Triticum aestivum L.) by improving physiological characteristics, growth, and water productivity

The impact of drought events on the growth and yield of wheat plants has been extensively reported; however, limited information is available on the changes in physiological characteristics and their effects on the growth and water productivity of wheat after repeated drought stimuli. Moreover, whether appropriate drought stimulus can improve stress resistance in plants by improving physiological traits remains to be explored. Thus, in this study, a pot experiment was conducted to investigate the effects of intermittent and persistent mild [65%–75% soil water-holding capacity (SWHC)], moderate (55%–65% SWHC), and severe drought (45%–55% SWHC) stress on the growth, physiological characteristics, yield, and water-use efficiency (WUE) of winter wheat. After the second stress stimulus, persistent severe drought stress resulted in 30.98%, 234.62%, 53.80%, and 31.00% reduction in leaf relative water content, leaf water potential, photosynthetic rate (Pn), and indole-3-acetic acid content (IAA), respectively, compared to the control plants. However, abscisic acid content, antioxidant enzyme activities, and osmoregulatory substance contents increased significantly under drought stress, especially under persistent drought stress. After the second rehydration stimulus (ASRR), the actual and maximum efficiency of PSII and leaf water status in the plants exposed to intermittent moderate drought (IS2) stress were restored to the control levels, resulting in Pn being 102.56% of the control values; instantaneous WUE of the plants exposed to persistent severe drought stress was 1.79 times that of the control plants. In addition, the activities of superoxide dismutase, peroxidase, catalase, and glutathione reductase, as well as the content of proline, under persistent mild drought stress increased by 52.98%, 33.47%, 51.95%, 52.35%, and 17.07% at ASRR, respectively, compared to the control plants, which provided continuous antioxidant protection to wheat plants. This was also demonstrated by the lower H2O2 and MDA contents after rehydration. At ASRR, the IAA content in the IS2 and persistent moderate drought treatments increased by 36.23% and 19.61%, respectively, compared to the control plants, which favored increased aboveground dry mass and plant height. Compared to the control plants, IS2 significantly increased wheat yield, WUE for grain yield, and WUE for biomass, by 10.15%, 32.94%, and 33.16%, respectively. Collectively, IS2 increased grain growth, yield, and WUE, which could be mainly attributed to improved physiological characteristics after drought-stimulated rehydration.