Effects Of Various Doses Research Articles

Effect of different doses of esketamine on the median effective concentration of propofol for inhibiting body movement during hysteroscopy

The objective of this study is to investigate the effects of various doses of esketamine on the median effective concentration (EC50) of propofol required for inhibiting body movement during hysteroscopy. Additionally, this research aims to explore the pharmacodynamic interactions between esketamine and propofol. Prospective, double-blind, up-down sequential allocation study. Operating room, post-anesthesia care unit (PACU), and general ward. A total of 90 patients were allocated into three groups in a randomized, double-blinded manner as follows: 0.1 mg/kg esketamine combined with propofol intravenous injection (EP0.1) group, 0.2 mg/kg esketamine combined with propofol intravenous injection (EP0.2) group, 0.3 mg/kg esketamine combined with propofol of intravenous injection (EP0.3) group. For the initial patient in each group, the starting effector target concentration of propofol was set at 4 µg/ml. Each patient received an initial intravenous injection of 0.04 mg/kg midazolam, followed by the administration of the appropriate dose of esketamine. Ten seconds after the esketamine injection, propofol was administered intravenously to achieve the target concentration. In accordance with the sequential method principle, the concentration of propofol for the subsequent patient was adjusted based on the response of the previous patient. Effective inhibition of body movement was defined as the absence of any involuntary body movements throughout the entire surgical process. If the previous patient exhibited body movements, the propofol concentration for the next patient was increased by 0.5 µg/ml; conversely, if no movements were observed, it was decreased by 0.5 µg/ml. The up-down sequential allocation method and probit regression were used to calculate the EC50 of propofol. Hospital Anxiety and Depression Scale-Anxiety (HADS-A) and Depression (HADS-D) score, adverse events, hemodynamic changes, demographic data and clinical characteristics. The EC50 of propofol was 3.849 μg/ml (95% CI: 3.419–4.281) in the EP0.1 group, 3.641 μg/ml (95% CI: 2.807−4.200) in the EP0.2 group, and 3.417 μg/ml (95% CI: 2.845–3.852) in the EP0.3 group. These findings suggest that esketamine can dose-dependently reduce the EC50 of propofol. Esketamine can dose-dependently reduce the EC50 of propofol in hysteroscopy, while concurrently lowering patients’ HADS-A and HADS-D scores 24 h post-operation. It is concluded that the optimal dose of esketamine, when combined with propofol for hysteroscopy, is 0.3 mg/kg.

Growth Rate and Production of Hybrid and Local Maize (Zea Mays L.) in Response to Various Doses of Nitrogen Fertilization

Corn (Zea mays L.) is a strategic commodity after rice so that the productivity need to be optimize through nitrogen fertilization. The aim of this research is to evaluate the effect of various doses of urea fertilizer on the growth rate and yield of hybrid corn and local corn. This research was conducted from June to October 2022 on the pilot land of the Agricultural Training Center (Bapeltan), Hajimena, Bandar Lampung. The factorial experiment (5x2) was arranged in a randomized complete block design with 3 replications. The first factor is urea fertilizer doses, namely: 200 kg/ha (N1), 250 kg/ha (N2), 300 kg/ha (N3), 350 kg/ha (N4) and 400 kg/ha (N5), while other fertilizers were given to all experimental plots in the same rate (150 SP-36 kg/ha and 100 kg KCl kg/ha). The second factor is the corn variety, included V1 (BISI-18) and V2 (Tongkol Merah) varieties. The results showed that increasing dose of urea from 200 to 400 kg/ha was not able to increase the growth rate of both corn varieties. The productivity of the BISI-18 corn variety is higher than that of the Tongkol Merah as indicated by the wet weight of the cobs (g), the dry weight of grains per plot (g), and the yield of corn dry grains (tons/ha). The yield of dry corn seeds for the BISI-18 corn variety was 11.7 tons/ha, while for the Tongkol Merah variety was 9.44 tons/ha. Keywords: Fertilizer dosage, Hybrid corn, Local corn, Nitrogen use efficiency, Yield.

Pyrite Depression by Sodium Metabisulfite in Freshwater and Seawater with Copper Activation

Pyrite has natural floatability and thus readily enters valuable mineral flotation concentrates, diluting their quality and decreasing their economic value. Its separation is challenging, depending on process conditions, the presence of activating ions, and water quality, particularly in regions where seawater is used. This study examines the effect of various doses of sodium metabisulfite (SMBS) on pyrite depression in freshwater and seawater under weakly alkaline conditions and with different copper ion concentrations. Without the addition of activators or depressants, pyrite recovery is 40% in freshwater and 60% in seawater, whereas with 10 ppm of SMBS, recoveries drop to 28% and 38%, respectively. The addition of 10 ppm Cu2+ increases recovery by 10% in freshwater and by 20% in seawater. In the presence of 75 ppm of PAX, maximum recovery reached 50% in freshwater and 80% in seawater. These results suggest that cationic bridges formed by seawater ions, combined with CaOH+ activation, play a critical role in pyrite activation, even in the presence of depressants.

Integrated lipidomic and transcriptomics to explore the effects of ethyl acetate extract of Herpetospermum pedunculosum on nonalcoholic fatty liver disease in mice

Ethnopharmacological relevanceHerpetospermum pedunculosum (Ser.) C.B. Clarke (HP), a traditional Tibetan medicine used to treat hepatobiliary diseases, was confirmed that lignans-enriched ethyl acetate extract of HP (EAHP) could alleviate the hepatic injury by modern pharmacological evidence. However, the effects and potential mechanisms of EAHP against nonalcoholic fatty liver disease (NAFLD) are still unknown. Aim of the studyTo reveal the effects of EAHP on NAFLD and explore the potential mechanisms from the perspective of lipidomics and transcriptomics. Materials and methodsUPLC‒Q–TOF‒MS analysis was carried out to investigate the chemical components of EAHP. A Choline-deficient, L-amino acid defined, high fat diet (CDAHFD) was used to establish a NAFLD mouse model. The anti-NAFLD effects of various dosages of EAHP were evaluated by biochemical indexes and histological analysis. Hepatic lipidomic and transcriptomic analysis and multiple bioinformatics methods were used to screen biomarkers and signaling pathways. The levels of the corresponding genes were verified by qPCR. Results36 kinds of compounds were identified by UPLC‒Q–TOF‒MS analysis. Oral treatment with EAHP significantly decrease the liver index and the levels of ALT and AST in the serum. The measurements lipid content and Oil Red O staining results suggested that EAHP ameliorated lipid metabolism disorders by reducing the content of TG and LDL-C, increasing HDL-C in the liver. H&E staining and ELISA revealed that EAHP restored hepatic inflammatory infiltration and decrease the levels of IL-1β, IL-6, TNF-α, and increase IL-10 in the serum. Lipidomic analysis showed that EAHP could regulate CDAHFD-induced lipid metabolic disorder. The different lipid metabolites included TG, phosphatidyl choline (PC), diacylglycerol (DG), phosphatidylethanolamine (PE), phosphatidylinositol (PI), ceramide (Cer). Transcriptomic analysis revealed that Bmp8b, Nbl1, Rgma, Sphk1, Thbs1, and Ugt8a were important regulators, which were associated with TGF-β signaling pathway and sphingolipid metabolism. The expressions of above genes detected by were qPCR consistent with transcriptomic data. ConclusionsThe ameliorative effects of EAHP on NAFLD are potentially attributable to the regulation of sphingolipid metabolism and TGF-β signaling pathway, etc., which results in abnormal hepatic lipid metabolism and inflammatory response.

Therapeutic potential of Rosa roxburghii folium extract in insomnia treatment: a comprehensive evaluation of behavioral and neurochemical effects in a PCPA-induced mouse model.

Insomnia, a prevalent sleep disorder, detrimentally affects quality of life and is often challenging to manage with conventional treatments. This study delved into identifying and quantifying the main compounds by employing ultra-performance liquid chromatography-Q-Exactive-Orbitrap mass spectrometry, and further to evaluate the therapeutic potential of Rosa roxburghii folium (RRF) extract, with multiple pharmacological activities. Previous research had hinted at the efficacy of glycosides in influencing the γ-aminobutyric acid (GABAergic) system, which plays a pivotal role in sleep regulation. Utilizing a p-chlorophenylalanine-induced insomnia model in BALB/C mice, this investigation aimed to unravel the effects of various dosages of RRF extract on sleep quality and elucidated its mechanism of action. A total of 66 compounds in the RRF extract were analyzed. Behavioral assessments demonstrated notable enhancements in sleep duration and latency. Biochemical analyses further corroborated these findings, revealing modulation in neurotransmitter levels indicative of a potential mechanism through the GABAergic and serotoninergic pathways. Additionally, histological evaluations suggested anti-inflammatory and antioxidant effects of the RRF extract. The findings from this study underscored the therapeutic efficacy of RRF extract in combating insomnia, particularly highlighting its glycoside components' role. The extract's significant improvement in sleep duration and latency, alongside its modulation of neurotransmitter levels, showcases its potential as a natural remedy for insomnia. Through its action on the GABAergic and serotoninergic pathways, as well as its anti-inflammatory and antioxidant effects, RRF extract emerges as a promising candidate for insomnia treatment, offering a holistic approach to sleep disorder management. © 2024 Society of Chemical Industry.

Growth of cocoa seedlings (Theobroma cacao L.) in application of cow manure compost and density of Bacillus subtilis bacteria

Cocoa rejuvenation is important to maintain high productivity. The rejuvenation mainly uses seedlings; thus providing high-quality seedlings is important. This research aimed to determine the effect of various doses of cow manure compost and the density of Bacillus subtilis bacteria on the growth of cocoa seedlings. The research was implemented at the Teaching Farm, Faculty of Agriculture, Hasanuddin University, Makassar City, from 2021 to 2022. The experiment was arranged in a split-plot design with three replications. The main plot was the dose of cow manure (without compost, 1.25 kg per polybag, and 2.5 kg per polybag). The subplot was the density of Bacillus subtilis (without bacteria, 104 CFU.mL-1, 108 CFU.mL-1, and 1012 CFU.mL-1). The results showed an interaction between the dose of cow manure 2.5 kg per polybag and the bacterial density 1012 CFU.mL-1 on chlorophyll a (347.91 mol.m-2), chlorophyll b (158.33 mol.m-2), total chlorophyll (444.57 mol.m-2), and leaf area of cocoa seedlings (813.96 cm2). A compost dose of 2.5 kg per polybag showed the best results for plant height (8.98 cm). Keywords: bacteria density; bivariate correlation; chlorophyll content; manure dose; organic fertilizer

Evaluations of the in vitro and in vivo antidiabetic activity of 70 % ethanolic fruit extracts of Rosa abyssinica

BackgroundDiabetes mellitus is becoming major health challenge with continually increasing burden. High costs of conventional medicines and numerous side effects associated with them, on the other hand, easy availability and accessibility of traditional herbal medicines calls upon experimental investigations to validate their effect on lowering blood glucose level. MethodsThe dried fruit of Rosa abyssinica was macerated with 70 % ethanol and the extract's in vitro antidiabetic activity was investigated using dinitrosalisylic acid method for alpha amylase inhibitory activity. Furthermore, the in vivo hypoglycemic and Antihyperglycemic effects of various doses of the extract (100, 200 and 400 mg/kg) was determined on normoglycemic, glucose loaded (1500 mg/kg) and Streptozotocine (180 mg/kg)-induced diabetic mice models. ResultsThe acute oral toxicity study revealed the plant showed no toxic effect on swiss albino mice at 2000 mg/kg. The in vitro alpha amylase inhibitory activity study showed that the extract has comparable IC50 value of 21.37 ± 4.252 μg/ml with the standard drug acarbose (IC50 value of 26.72 ± 3.59 μg/ml). On the other hand, in normal mice, none of the dose levels except at 400 mg/kg significantly reduces blood glucose level. This is in contrast to the oral glucose tolerance test, which the extract produced significant reduction at 60, 90 and 120 min following glucose challenge. The 70 % ethanolic fruit extracts of Rosa abyssinica also experienced profound antidiabetic activity in streptozotocin-induced diabetic model. In the single-dose study, both RAFE200 and RAFE400 demonstrated a significant (P˂0.05) reduction in blood glucose levels at 1, 2, 3, and 4 h. Similarly, in the repeated-dose study, RAFE200 and RAFE400 not only significantly reduced blood glucose levels but also produced a notable improvement in animal body weight. ConclusionThe 70 % ethanolic fruit extracts of Rosa abyssinica have shown significant in vitro alpha amylase inhibition effect and an in vivo blood glucose level lowering effects in diabetic mice.Therefore, this study supports the traditional use of Rosa abyssinica in the management of diabetes mellitus.

Effect of different doses of Budesonide combined with Tiotropium bromide inhalation on elderly patients with chronic obstructive pulmonary disease.

To explore the clinical effect of various doses of Budesonide combined with Tiotropium bromide in the treatment of elderly patients with chronic obstructive pulmonary disease (COPD). Clinical data of elderly patients with COPD, admitted to Affiliated Hospital of Shaoxing University from April 2021 to February 2023, were retrospectively analyzed. Based on the dosage of Budesonide combined with Tiotropium bromide, patients were divided into Low-dose group (Budesonide = 1mg), Medium-dose group (Budesonide = 2mg), and High-dose group (Budesonide = 3mg). All groups were matched for age, gender, course of disease, and BMI. Patients treated with Tiotropium bromide alone were assigned to the Control group. The clinical effect, pulmonary function index level, symptom improvement, inflammatory factor index level and adverse reactions in all groups were analyzed and compared. A total of 88 patients were included in this study with 22 patients in each group. The total efficacy of Medium-dose (90.91%) and High-dose group (90.91%) was significantly higher than that of Low-dose group (63.64%) and the Control group (59.09%) (P<0.05). After the treatment, levels of pulmonary function, symptom improvement and inflammatory factors in the High-dose and the Medium-dose groups were better than those in the Low-dose group and the Control group. Pulmonary function, symptom improvement and levels of inflammatory factors was significantly better in the Low-dose group compared to the Control group (P<0.05). Budesonide combined with tiotropium bromide is better than tiotropium bromide alone in the treatment of elderly patients with COPD. Compared with low (1mg) dosage, medium (2mg) and high (3mg) dosage of budesonide are more effective in improving lung function, alleviating symptoms, reducing inflammatory response,, and are not associated with increased rate of adverse reactions.

The effect of different doses of ammonium nitrate on the yield and protein content in spring triticale grain

The article presents the results of studying the effect of various doses of ammonium nitrate on the yield and protein content in spring triticale grain. Numerous studies around the world have established that nitrogen is a key factor influencing the productivity of grain crops. The paper presents the results of a 5-year study (2018-2022) of cultivating spring triticale using fallow and stubble predecessors. Nitrogen fertilizer was applied when sowing in rows at a dose of 20 to 80 kg/ha in the active substance. The experiments were carried out in the Akmola region (LLP "SPCGF named after A. Barayev") on southern carbonate chernozem. Over the five years of research, only 2018 was characterized by optimal hydrothermal conditions during the triticale growing season, the remaining four years (2019-2022) were dry. The reserves of productive moisture in the meter layer of soil before sowing triticale in fallow were 119-138 mm, in stubble - 119-125 mm. The content of N-NO3 in the soil layer 0-40 cm before sowing triticale varied according to the years of research, so for fallow it varied from 11 to 28 mg/kg, and for stubble from 10 to 17 mg/kg of soil. The amount of P2O5 before sowing (in the soil layer of 0-20 cm), regardless of the predecessor, varied within 24-37 mg/kg of soil. It was not possible to establish a reliable effect of different doses of ammonium nitrate on grain yield and protein content in triticale grain using the two studied precursors. A strong correlation between the dose of nitrogen fertilizer and the yield of triticale based on the steam predecessor was established only in 2020 (r = 0.85) and in 2021 (r = 0.83). For stubble, a strong positive correlation between nitrogen doses and yield was noted in 2019 (r = 0.78), 2021 (r = 0.79) and 2022 (r = 0.87). A correlation between the protein content in triticale grain and nitrogen doses was established based on the fallow in 2 of 4 years (r = 0.86...0.96). For the stubble, a strong positive relationship was noted in all years of research (r = 0.83...0.99).

The morphophysiological and yield characteristics of biquinho amarelo pepper in response to bovine rumen residue and chicken litter in soil are dose dependent

In response to the growing demand for environmentally sustainable agricultural practices that enhance production efficiency, this study aimed to determine the optimal application rates of bovine rumen content (CBR) and chicken litter (CCL) as organic composts. Conducted in a controlled greenhouse environment, the research assessed the effects of various doses of these composts on soil chemical and microbiological properties and on the growth and productivity of yellow biquinho pepper (Capsicum chinense Jacq.). The results of this study confirm that organic composts improve soil health and increase the growth and productivity of yellow biquinho pepper. However, while CBR consistently enhances plant growth across a range of doses, CCL shows a dose-dependent response, with the highest fruit production achieved at an application rate of 73.92 Mg ha−1. Although this dose of CCL is beneficial for increasing yield, it also marks a threshold beyond which further increases may lead to negative effects such as nutrient saturation or salt accumulation, potentially resulting in soil toxicity. The study supports the use of CBR and CCL as effective soil conditioners and emphasizes the need to identify the optimal compost dose to efficiently harness the benefits of organic composts in sustainable agricultural practices.

PEMBERIAN PUPUK ORGANIK CAIR ECENG GONDOK TERHADAP HASIL CABAI RAWIT (Capsicum frutescent L.)

ABSTRACTLiquid organic fertilizer (LOF) is proven to be more effective than solid organic fertilizer because nutrient mineralization is more efficient, so nutrients are quickly available for plants. Water hyacinth, as a water weed, turns out to have many functions. Besides being able to become a biofilter by absorbing chemical compounds in polluted water, the accumulation results also contain mineral compounds that are useful for increasing plant yields. Therefore, it is necessary to review the results of applying LOF made from water hyacinth to cayenne pepper plants. This study aims to analyze the effect of various doses of water hyacinth extract on the yield of cayenne pepper. This study uses a non-factorial experimental method, a complete random design (CRD), with an analysis model of LSD test (Least Significant Difference Test) α 5%. There are 4 treatments, namely, 0 ml, 40 ml, 60 ml, and 80 ml, with 5 replications. The parameters observed are the number of fruits, the fruit weight, and the wet weight of the plant. The results showed that the water hyacinth LOF has a pH of 7, doesn’t sting, and is light brown. The best treatment for P3 or 80 ml of water hyacinth POC is that the average number of fruits is 10 seeds, the average weight of fruit is 2.04 grams, and the average wet weight of plants is 46.9 grams.

Impact of Cultural Practices and Nitrogenous Fertilizer on the Development of Brown Spot Disease of Rice

Among the diseases the brown spot disease of rice caused by Bipolaris oryzae is most dominant fungal disease of rice after blast (Pyricularia oryzae) which is causing losses in the yield which resulted in instability in rice production particularly in north Bihar. This disease was considered to be one of the most important facet of 1943 Bengal Famine. The losses in famine were accounted to 50-90 per cent (Ghose et al. 1960; Padmanabhan, 1973). The disease is a serious and has global distribution and it has been found in all rice growing countries in Asia, America and Africa. The studies mainly adhered to see the effect of various doses of nitrogen and different cultural practices on development of brown spot disease of rice. Among the various cultural practices, minimum (17.64%) disease seventy and maximum yield (3.13/ha) were found with application of FYM (5 t/ha) + line sowing of 60 kg seed/ha + 50% N application after 15 DAS 25 % N at maximum tillering + 25% N at panicle initiation + weed control. Doses of nitrogen were positively related with the disease development and negatively related with the grain yield indicating the maximum (38.30 % and 39.0 %) disease severity and minimum (1.86 and 1.97 t/ha) grain yield with nitrogen dose of 120 kg/ha. In respect of varietal response, the minimum (12.10 % and 16.40 %) disease severity and the maximum (4.34 t/ha and 4.58 t/ha) yield were recorded in the variety Rajshree receiving 80 kg N/ha.

Heavy ion beams and gamma rays induce biological changes and flavonoid accumulation in Astragalus mongholicus Bunge

Astragalus mongholicus Bunge (A. mongholicus) is a perennial herb in the Fabaceae family. Its dried rhizome has been used in clinical practice in China for more than 2000 years. However, the limited germplasm resources and unstable quality of A. mongholicus have limited its large-scale cultivation and wide application. Therefore, there is an urgent need to breed stable varieties. At present, heavy ion beams (HIBs) and gamma rays are important mutagenesis tools applied in medicinal plants. The aim of this investigation was to assess the effects of various doses (0, 50, 100, 200, and 400 Gy) of HIBs and gamma rays on the development, physiological reaction, and secondary metabolite accumulation of A. mongholicus. The findings indicated that the LD50 of the A. mongholicus seeds to HIBs and gamma rays was 200–250 Gy and 400 Gy, respectively. As the radiation dose increased, there was a subsequent increase in the inhibition of seedling height, plant diameter, and number of branches and leaves, as well as a decrease in the chlorophyll a, b, and carotenoid contents of leaves. Additionally, the chlorophyll fluorescence parameter NPQ and flavonoid contents (total flavonoids, Calycosin-7-glucoside, Calycosin, Ononin, and Methylnissolin-3-O-glucoside) were also affected, which resulted in a distinct decrease in Y(II) values and diverse levels of elevation in leaf MDA content, SOD, and POD activities. Differently, CAT activity was substantially reduced, except for 50 Gy HIBs irradiation. Overall, upon the same dose of the two radiation qualities, HIBs had a stronger inhibitory effect on the growth of Astragalus seedlings, but less effect on antioxidant enzymes and physiological responses. There was no difference between two radiation qualities in increasing trend of flavonoid composition. The present study showed that HIBs is more beneficial for A. membranaceus mutation breeding research and would be promising tool for A. mongholicus mutation breeding.

Neurokinin B Administration Induces Dose Dependent Proliferation of Seminal Vesicles in Adult Rats.

Neurokinin B; an endogenous decapeptide, mediates its reproductive physiological actions through gonadotropin releasing hormone. Despite the potential role of Neurokinin B on seminal vesicles, its effects on seminal vesicles in adult male mammals remain elusive. We aimed to investigate the potentials of variable doses of Neurokinin B, its agonist and antagonist on histomorphology and expression of NK3R on seminal vesicles, and secretory activity of seminal vesicles in adult male rats. Adult male Sprague Dawley rats (n=10 in each group) were administered intraperitoneally with Neurokinin B in three variable doses: 1 μg, 1 ηg and 10 ρg while, Senktide (Neurokinin B agonist) and SB222200 (Neurokinin B antagonist) in 1 μg doses consecutively for 12 days. After 12 days of peptide treatment, half of the animals (n=05) in each group were sacrificed while remaining half (n=05) were kept for another 12 days without any treatment to investigate treatment reversal. Seminal vesicles were dissected and excised tissue was processed for light microscopy, immunohistochemistry and estimation of seminal fructose levels. Treatment with Neurokinin B and Senktide significantly increased while SB222200 slightly decrease the seminal vesicles weight, epithelial height and seminal fructose levels as compared to control. Light microscopy revealed increased epithelial height and epithelial folding as compared to control in all Neurokinin B and Senktide treated groups while decreased in SB222200. Effects of various doses of Neurokinin B, Senktide and SB222200 on seminal vesicles weight, epithelial height, seminal fructose levels and histomorphology were reversed when rats were maintained without treatments. Immuno-expression of Neurokinin B shows no change in treatment and reversal groups. Continuous administration of Neurokinin B and Senktide effect positively while SB222200 have detrimental effects on cellular morphology, epithelial height and seminal fructose levels in seminal vesicles. Effects of peptide treatments depicted a reversal towards control group when rats were kept without any treatment.

The Vasodilator Effect and Mechanism of 18β-glycyrrhetinic Acid on Isolated Pulmonary Artery Rings in Rats

Background: The purpose of this study was to evaluate the effect of 18β-glycyrrhetinic acid (18β-GA) on isolated rat pulmonary artery vascular rings as well as the mechanism that lies behind the diastolic effects of this compound. Methods: To examine the effects of various doses of 18β-GA on resting normal vascular rings, we used isolated rat pulmonary artery vascular rings. The isolated rat pulmonary artery vascular ring was precontracted with phenylephrine (PE) and potassium chloride solution (KCl), which allowed for the observation of the diastolic effects of 18β-GA, as well as the effects of various concentrations of 18β-GA on blocking the four potassium channels of glibenclamide, barium chloride (BaCl2), tetraethylamine (TEA), or 4-aminopyridine; the impact of various 18β-GA concentrations on the pulmonary artery vasodilation effect pre-use of the nitric oxide synthase inhibitors l-nitroarginine methyl ester and indomethacin. Results: It was shown that 18β-GA has concentration-dependent diastolic effects on isolated rat pulmonary vascular rings that have been precontracted with PE and KCl but it has no effect on resting isolated thoracic aortic vascular rings. Conclusion: The two Kir and Kv channels may be connected to the endothelium-dependent vasodilation mechanism of 18β-GA.

Use of kersetin and fisetin flavonoids in combination with pregabalin and gabapentin in the treatment of neuropathic pain.

We purposed to explore the consequences of the use quercetin and fisetin alone and in combination with pregabalin and gabapentin, which are used in the management of neuropathic pain, and on neuropathic pain in general. The anti-allodynic effect of various doses (5, 10, and 20 mg/kg) of quercetin and fisetin, both singly and in combination with pregabalin and gabapentin, was evaluated by developing a neuropathic pain model induced by chronic constrictive nerve damage in rats. The effectiveness of these flavonoids was investigated by combining them with gabapentin (50 mg/kg) and pregabalin (15 mg/kg), choosing the effectual dose of 10 mg/kg and the dose of 5 mg/kg, which did not show significant antiallodynic effects. In groups combined with gabapentin and pregabalin, it was determined that they showed a significant antiallodynic effect compared with 50 mg/kg gabapentin and 15 mg/kg pregabalin. In conclusion, in our combination studies, it was observed that the effectiveness of gabapentin and pregabalin, was increased and the duration of effect was prolonged when used with lower doses of flavonoids. Based on these findings; it is possible to say that quercetin and fisetin are potential agents that can be used alone or in combination with other effective treatments to alleviate neuropathic pain.

Effect of various doses of nitrogen based on organic fertilizer enriched with Leucaena leucocephala leaves on the growth and yield of three rice varieties

Effect of various doses of nitrogen based on organic fertilizer enriched with Leucaena leucocephala leaves on the growth and yield of three rice varieties

Growth and Production of Romaine Lettuce (Lactuca sativa L.) on Several Dosage of Rabbit Urine

Romaine lettuce is a vegetable with high economic value. Romaine lettuce needs to be grown organically. This research aimed to study the effect of various doses of rabbit urine on romaine lettuce. This research was conducted from June to August 2022 at the Smart and Urban Farming Laboratory Greenhouse, Campus F7, Gunadarma University, East Jakarta, Indonesia. The study used a randomized block design (RBD) with five replications, consisting of 5 treatments, namely: P0: without rabbit urine (control); P1: Concentration of rabbit urine 25%; P2: Concentration of rabbit urine 50%; P3: Concentration of rabbit urine 75%; P4: Concentration of rabbit urine 100%. The results showed that the administration of rabbit urine had no significant effect on the growth and yield of romaine lettuce plants, and the best concentration of rabbit urine for romaine lettuce plants in this study was 80 ml (P2).

Effects of Various Caffeine Doses on Cognitive Abilities in Female Athletes with Low Caffeine Consumption.

Caffeine (CAF), a prevalent psychoactive stimulant, is believed to potentially enhance cognitive ability. However, studies on the effects of various doses are limited and yield inconsistent results, particularly in female athletes. Therefore, we aimed to assess the association between three different dosages of CAF intake (low, moderate, and high) and cognitive skills in female athletes with low CAF consumption. This study had a randomized, crossover, double-blind design in which each athlete performed four experimental sessions after ingesting either a placebo (PLAC), 3 mg·kg-1 of CAF (3 mg of CAF), 6 mg·kg-1 of CAF (6 mg of CAF), or 9 mg·kg-1 of CAF (9 mg of CAF) with an in-between washout period of at least 72 h. Following a 60 min window post-capsule consumption, fourteen female athletes (age: 17.4 ± 0.8 years) were assessed through various cognitive tests, namely, simple reaction time (SRT), choice reaction time (CRT), and attentional task (AT) tests, along with the mental rotation test (MRT). Additionally, they were required to complete a questionnaire about the undesirable side effects of CAF. Our results indicated that, compared to those of PLAC, the SRT, CRT, and AT performance were significantly improved following the administration of both 3 mg of CAF and 6 mg of CAF. While the greatest enhancement was observed after consuming 3 mg of CAF, no significant differences were found between the effects of 3 mg and 6 mg of CAF. Interestingly, MRT performance did not improve with any of the CAF dosages. Moreover, the ingestion of 9 mg of CAF did not enhance cognitive skills and was linked to the highest occurrence of CAF-related side effects. In conclusion, our results highlight the recommendation for a low CAF dosage of 3 mg·kg-1, in contrast to a higher dose of 6 mg·kg-1 or 9 mg·kg-1 of CAF, to enhance various aspects of cognitive performance in female athletes with low CAF consumption without adverse side effects.

Optimization of Phosphorus Application Rate on Maize Under Agro-ecological Condition of Urozgan

Phosphorus plays a vital role in crop growth and productivity, thus a field experiment entitled (optimization of phosphorus application rate on maize under agro-ecological condition of Urozgan) was conducted at Urozgan Higher Education Institute research farm, during spring season of 2023 to evaluate the effects of various doses of phosphorus on maize growth and yield. Treatments were consisted of four levels of phosphorus 20, 40, 60, 80 Kg P2O5 ha-1 and control, the experiment was laid out in randomized complete block design and replicated thrice, the data was analyzed by IBM SPSS 24. Phosphorus application significantly influenced maize growth, yield and yield attributes compared to control, while the phosphorus doses increased from 20 to 80 kg P2O5 ha-1, crop growth, yield, and yield attribute were continuously increased. It can be concluded from the result of experiment that application of phosphorus under agro-ecological condition of Urozgan can increase crop growth, yield and yield attributes.