Objective To explore the effect of nursing intervention on the biofeedback effect of functional constipation in children. Methods A total of 112 children (4-16 years old) who were diagnosed with functional constipation in the Department of Pediatric Surgery of the First Affiliated Hospital of Zhengzhou University were randomly divided into the experimental group (54 cases) and the control group (53 cases). Children in the experimental group were treated with biofeedback training and personalized nursing intervention for functional constipation in children. The control group used biofeedback combined with traditional nursing intervention to treat functional constipation in children. The WHO-5 (World Health Organization′s five physical and mental health) scales were used to compare the effects of the two groups and were followed up for 3-6 months. Results A total of 107 children completed the study, 54 in the experimental group and 53 in the control group. There were no significant differences in the scores of the WHO-5 scale and clinical symptoms between the two groups before the intervention (P>0.05). After intervention, the total score of WHO-5 in the experimental group was (21.69± 2.28), the total score of clinical symptoms was (6.56±1.16), the total score of WHO-5 in the control group was (17.85±3.189), and the total score of clinical symptoms was (9.04±2.121). The difference was significant (t=-7.146, t=7.491, P<0.01). Conclusion Personalized nursing intervention can enhance the effect of biofeedback training on children and adolescents with functional constipation and improve their quality of life. Key words: Nursing intervention; Functional constipation in children; Biofeedback