Effect Of Medium Composition Research Articles

The impact of media composition on production of flavonoids in agitated shoot cultures of the three Hypericum perforatum L. cultivars \u2018Elixir,\u2019 \u2018Helos,\u2019 and \u2018Topas\u2019

The aims of the study were to evaluate the effect of media composition on the growth potential and morphology of the in vitro cultured biomass of three cultivars of Hypericum perforatum, and on the production of flavonoids. Agitated shoot cultures were maintained in parallel on Linsmaier and Skoog (LS) and Murashige and Skoog (MS) media supplemented with 0.1–3.0 mg L−1 of α-naphthaleneacetic acid and 6-benzylaminopurine. Methanolic extracts from the biomass collected after 3-wk growth cycles were analyzed quantitatively, for 21 flavonoids using high performance liquid chromatography. Three aglycones (kaempferol, luteolin, and quercetin) and three glycosides of quercetin (hyperoside, quercitrin, and rutoside) were detected in all of the extracts. The total amounts of the estimated compounds increased from 1.18- to 21.66-fold on LS media variants and from 1.52- to 17.34-fold on MS media variants. The main metabolite was quercetin (max. 210.55 mg 100 g−1 dry weight [DW]). The maximum total amounts of all compounds in the biomass of ‘Elixir,’ ‘Helos,’ and ‘Topas’ were 328.53, 255.70, and 166.58 mg 100 g−1 DW, respectively. The shoots of all cultivars cultivated on the LS and MS media containing low levels of plant growth regulators (0.1 mg L−1) accumulated high amounts of flavonoids. The highest amounts were accumulated in shoots of cultivar ‘Elixir’ grown on MS medium. This is the first comparison of flavonoid production in three cultivars of H. perforatum (‘Elixir,’ ‘Helos,’ and ‘Topas’) cultured in vitro, and the first report of flavonoid production in cultivars ‘Elixir’ and ‘Helos.’

World market and biotechnological production of itaconic acid.

The itaconic acid (IA) world market is expected to exceed 216 million of dollars by 2020 as a result of an increasing demand for bio-based chemicals. The potential of this organic acid produced by fermentation mainly with filamentous fungi relies on the vast industrial applications of polymers derived from it. The applications may be as a superabsorbent polymer for personal care or agriculture, unsaturated polyester resin for the transportation industry, poly(methyl methacrylate) for electronic devices, among many others. However, the existence of other substitutes and the high production cost limit the current IA market. IA manufacturing is done mainly in China and other Asia-Pacific countries. Higher economic feasibility and production worldwide may be achieved with the use of low-cost feedstock of local origin and with the development of applications targeted to specific local markets. Moreover, research on the biological pathway for IA synthesis and the effect of medium composition are important for amplifying the knowledge about the production of that biochemical with great market potential.

Effect of Media Composition on Vegetative and Reproductive Growth of Alternaria Brassicicola and Bipolaris Sorokiniana

Experiments were conducted to evaluate the effect of some culture media on the vegetative and reproductive growth of Alternaria brassicicola and Bipolaris sorokiniana. Twenty one (21) treatment combinations each with three replications were employed. Potato Dextrose Agar (PDA) was used as the basic growth medium in this study. Supplementation with different plant extracts with this medium produced differential growth and sporulation of the tested fungi. PDA media supplemented with mustard leaf extract showed significantly highest vegetative growth (7.8 cm) and PDA with the combination of mustard leaf, tomato fruit, carrot fruit and cabbage leaf extracts showed the highest sporulation (11×105 spores/ml) of A. brassicicola. In case of B. sorokiniana, the highest vegetative growth (7.4 cm) and highest sporulation (45×104 spores/ml) were obtained by the supplementation of mustard and wheat leaf extracts respectively. Plant extract supplementation proved better than PDA as growth medium of A. brassicicola and B. sorokiniana.

Adjustment of medium composition and iso-osmotic potential in direct-shoot organogenesis produces true-to-type oil palm (Elaeis guineensis Jacq.) plantlets

Clonally propagating elite lines of oil palm via multiple shoot organogenesis is promising due to genetic stability of regenerants. In this study, we tested the effects of medium composition using three basal media types (Murashige and Skoog (MS), woody plant medium (WPM), and Y3) with concentrations of sucrose and different ionic strengths derived from sucrose, sorbitol, or both sucrose and sorbitol on the growth and development of oil palm plantlets derived from direct-shoot organogenesis and zygotic embryo-derived seedlings (control). We used histological assays to better understand the medium formulation’s efficiency for shoot organogenesis. The shoot induction percentage was the highest when plantlets were cultured on ½ MS medium supplemented with 5% sucrose. A minimum osmotic potential of -2.0 MPa is essential to efficiently drive shoot maturation of oil palm. Sorbitol appeared to be less effective than sucrose for shoot formation of oil palms. To determine the rate of somoclonal changes between these two micropropagation methods, we investigated the rate of DNA methylation in oil palm plantlets using the methylation-sensitive amplification polymorphism (MSAP) technique. Our MSAP results showed that polymorphisms between in vitro seedling-derived plantlets and direct-shoot organogenesis-derived plantlets were considerably low. We conclude that regenerated plantlets derived from this established protocol are reliable for trueto- type propagation of elite oil palm clones.

Production of arabitol from enzymatic hydrolysate of soybean flour by Debaryomyces hansenii fermentation

Arabitol is a low-calorie sugar alcohol with anti-cariogenic properties. Enzymatic hydrolysate of soybean flour is a new renewable biorefinery feedstock containing hexose, pentose, and organic nitrogen sources. Arabitol production by Debaryomyces hansenii using soybean flour hydrolysate was investigated. Effects of medium composition, operating conditions, and culture stage (growing or stationary phase) were studied. Production was also compared at different culture volumes to understand the effect of dissolved oxygen concentration (DO). Main factors examined for medium composition effects were the carbon to nitrogen concentration ratio (C/N), inorganic (ammonium) to organic nitrogen ratio (I/O-N), and sugar composition. Arabitol yield increased with increasing C/N ratio and a high I/O-N (0.8-1.0), suggesting higher yield at stationary phase of low pH (3.5-4.5). Catabolite repression was observed, with the following order of consumption: glucose > fructose > galactose > xylose > arabinose. Arabitol production also favored hexoses and, among hexoses, glucose. DO condition was of critical importance to arabitol production and cell metabolism. The yeast consumed pentoses (xylose and arabinose) only at more favorable DO conditions. Finally, arabitol was produced in fermentors using mixed hydrolysates of soy flour and hulls. The process gave an arabitol yield of 54%, volumetric productivity of 0.90g/L-h, and specific productivity of 0.031g/g-h.

Isolation, cultivation, and characterization of human mesenchymal stem cells.

Mesenchymal stem cells (MSC) exhibit a high self-renewal capacity, multilineage differentiation potential and immunomodulatory properties. This set of exceptional features makes them an attractive tool for research and clinical application. However, MSC are far from being a uniform cell type, which makes standardization difficult. The exact properties of human MSC (hMSC) can vary greatly depending on multiple parameters including tissue source, isolation method and medium composition. In this review we address the most important influence factors. We highlight variations in the differentiation potential of MSC from different tissue sources. Furthermore, we compare enzymatic isolation strategies with explants cultures focusing on adipose tissue and umbilical cords as two relevant examples. Additionally, we address effects of medium composition and serum supplementation on MSC expansion and differentiation. The lack of standardized methods for hMSC isolation and cultivation mandates careful evaluation of different protocols regarding efficiency and cell quality. MSC characterization based on a set of minimal criteria defined by the International Society for Cellular Therapy is a widely accepted practice, and additional testing for MSC functionality can provide valuable supplementary information. The MSC secretome has been identified as an important signaling mechanism to affect other cells. In this context, extracellular vesicles (EVs) are attracting increasing interest. The thorough characterization of MSC-derived EVs and their interaction with target cells is a crucial step toward a more complete understanding of MSC-derived EV functionality. Here, we focus on flow cytometric approaches to characterize free as well as cell bound EVs and address potential differences in the bioactivity of EVs derived from stem cells from different sources. © 2017 International Society for Advancement of Cytometry.

Ferrous Ion and Medium Composition Effects on Acidogenic Phase in Biobutanol Production from Molasses

Clostridium acetobutylicum B530 has ability to convert sugar into biobutanol through two phases, i.e. acidogenic and solventogenic. This fermentation process is often hampered by high raw material cost and low product yield. In order to suppress the production cost, the molasses, a byproduct of sugar cane process production, was used as carbon source in this research. Molasses has nitrogen content in a small amount, thus could be negating the beef extract component, which is expected not to affect the growth of C. acetobutylicum B530 and also can reduce the production cost. In addition, a certain amount of Fe2+ (ferrous ion), a precursor in the formation of the enzyme ferredoxin, was added to the fermentation medium to contribute in the synthesis of acetyl-CoA, so that the formation of acidogenic products such as butyric acid and acetic acid is affected. This study aimed to investigate the effect of ferrous ion and the medium composition in acidogenic phase. The addition of 20 ppm FeSO4.7H2O in the fermentation medium without beef extract can increase the concentration of butyric acid by 20% at a temperature of 35°C, while acetic acid concentration decreased by 6%. According to those results, it is expected that the product selectivity of butanol will increase in solventogenic phase. In addition, the removal of beef extract in the fermentation medium does not affect the kinetics of growth of C. acetobutylicum B530.

Isolation and evaluation of cultivation conditions of Euglena sp. from Western Himalaya for biofuel production

ABSTRACTThis study aims to isolate the unicellular green alga Euglena sp. from wastewater collected from river Rispana of Dehradun, Uttarakhand, India. The optimization of different environmental factors for biomass production was performed. The physico-chemical factors significantly affect the culture growth and biomass of the microalga, and therefore optimization of these parameters is important for the commercialization of microalgae based biofuels. In this research, the collective effect of media composition, light intensity, photoperiod, temperature and pH was studied on the biomass of the isolated strain. The maximum biomass (0.838 g L−1) was obtained in the blue-green (BG-11) medium under a light intensity of 120 µmol m−2 sec−1 and a 16:8 light: dark cycle at 25°C. The life cycle of Euglena sp. was also assessed. The life cycle of the Euglena sp. varied from 12 to 20 days but after the 10th day the culture undergoes the stationary phase of the life cycle. Euglena sp. may be evaluated for its potential as a biofuel feed stock.

Evaluating the effect of medium composition and fermentation conditions on microbial oil production by a Fusarium strain isolated from the open ocean

AbstractAs an important feedstock for biodiesel production, microbial oil has attracted considerable interest in recent years. Here, we investigated the effect of medium composition and culture conditions on cell growth and lipid accumulation of an oleaginous fungus

Effect of media composition on citrinin and bio-pigments production by Monascus ruber

Monascus species are used for production of red bio-pigments, which are used as food coloring agents. However, presence of the mycotoxin citrinin, which is a secondary metabolite produced in conjunction with the red pigments, prevents the use of these pigments in large scale. In the present study, Monascus ruber Van Tieghem was isolated from the Egyptian soil and identified. Effect of media composition on nine of the pigments produced by this fungus in addition to citrinin was studied. The fungus was grown in four different media containing different carbon sources (glucose, ethanol, yeast malt extract and potato dextrose broth). On the other hand, effect of glucose concentration was also studied. The produced pigments and citrinin were extracted by butnol and analyzed by LC/MS/MS as well as HPLC with fluorescence detection of citrinin. The data revealed that the highest amount of red pigments production (4.3g/l) was obtained at glucose concentration 10g/l. This was combined with complete absence of citrinin. Rubropunctamine (26.1%) and ankuflavine (48.7%) were the most abundant pigments in the glucose and ethanol media, respectively; while Moanascin (61.6%) and N-glutarylrubropunctamine (39.4%) were the most abundant in the PDB and YMB media, respectively. This result enables the large scale production of the red pigments by M. ruber without production of the mycotoxin citrinin.

Nutritional Requirements for Mycelial Growth of Milk-White Toothed Mushroom, Irpex lacteus (Agaricomycetes), in Submerged Culture.

Irpex lacteus, a medicinal fungus, is used in traditional Chinese medicine to treat chronic glomerulonephritis. In this work, a strain of I. lacteus was isolated from the fruiting body of a wild specimen and identified by ITS-5.8S ribosomal DNA sequencing analysis. Then the nutritional requirements and culture conditions for mycelial growth of I. lacteus in semisynthetic liquid media were investigated using the one-factor-at-a-time and orthogonal matrix methods. Optimum growth occurred at 30°C and 35°C. I. lacteus mycelia grew well at pH values between 3 and 9, suggesting that this strain is not sensitive to pH. The nutritional components, including 9 carbohydrates, 9 nitrogen compounds, 11 vitamins, and 10 mineral elements, were studied for their effects on mycelial growth in submerged cultures of I. lacteus. Among these variables, soluble starch, peptone, yeast extract, and calcium chloride were identified as required for optimum mycelial growth. The concentrations of each component were optimized using an orthogonal design, and the effects of medium composition on mycelial growth were found in the order soluble starch > yeast extract > peptone > calcium chloride. The optimal concentrations of these components for mycelial growth were determined to be 60 g/L soluble starch, 35 g/L peptone, 15 g/L yeast extract, and 0.6 g/L calcium chloride. Under the optimum medium and culture conditions, the maximum biomass reached 13.73 g/L after 3 days in submerged culture, a value over twice that reached using the basal medium. These results provide a basis for further physiological study and industrial fermentation of I. lacteus.

Effect of media composition on bioavailability and toxicity of silver and silver nanoparticles in fish intestinal cells (RTgutGC)

To understand conditions affecting bioavailability and toxicity of citrate-coated silver nanoparticles (cit-AgNP) and dissolved silver at the luminal enterocyte interface, we exposed rainbow trout (Oncorhynchus mykiss) gut cells (RTgutGC) in media of contrasting composition: two amino acid-containing media, one of which was supplemented with proteins, as can be expected during digestion; and two protein and amino acid-free media contrasting low and high chloride content, as can be expected in the lumen of fish adapting to freshwater or seawater, respectively. Dose–response curves were generated measuring cell metabolic activity, membrane and lysosome integrity over a period of 72 hours. Then, nontoxic doses were applied and total silver accumulation, metallothionein and glutathione reductase mRNA levels were determined. The presence of proteins stabilized cit-AgNP keeping them in suspension. Conversely, in protein-free media, cit-AgNP agglomerated and settled, resulting in higher cellular accumulation of silver and toxicity. Chloride concentrations in exposure media modulated the toxicity of AgNO3 but not of cit-AgNP. Moreover, while amino acid-containing media are protective against AgNO3, likely due to the formation of thiolate complexes, they are only partially protective against cit-AgNP. Viability assays indicated that lysosomes are targets of cit-AgNP, supporting the hypothesis that cit-AgNP exert toxicity intracellularly. Metallothionein, a sensor of metal bioavailability, was induced by cit-AgNP in high chloride medium but not in low chloride medium, indicating that chloride might have a role in mobilizing silver from intercellular vesicles. Overall, this study shows that AgNP bioavailability and toxicity in the intestine is linked to its luminal content.

The effect of medium composition on deposition of collagen type 1 and expression of osteogenic genes in mesenchymal stem cells derived from human adipose tissue and bone marrow

Abstract The two mesenchymal stem cell (MSC) populations that have gained most attention in relation to bone tissue engineering are adipose tissue (AT) MSCs and bone marrow (BM) MSCs. The purpose of this study was to investigate the ability of human BM-MSCs and AT-MSCs to survive, proliferate and deposit collagen type 1 when cultured on polycaprolactone nanofiber scaffolds and to ascertain the effect of medium composition on collagen type 1 formation and expression of osteogenic genes. The cells were seeded on polycaprolactone nanofiber scaffolds and cultured in three different types of media that differed by the presence of ascorbic acid, β-glycerophosphate and dexamethasone, that are typical components used for osteogenic differentiation of MSCs in vitro. In summary, AT-MSCs were proliferating significantly faster than BM-MSCs. AT-MSCs also showed better ability to deposit collagen type 1 and had a higher expression of early osteogenic markers, whereas BM-MSCs had higher expression of late osteogenic markers. This suggests that MSCs from diverse sources have different attributes and with respect to osteogenic differentiation, AT-MSCs are more immature compared to BM-MSCs. Collagen formation was depending on medium composition and the organization of collagen type 1 appeared to be influenced by the presence of dexamethasone.

Effects of Medium Compositions on Organogenesis in the In Vitro Cultured Diploid and Tetraploid Codonopsis lanceolata

Effects of Medium Compositions on Organogenesis in the In Vitro Cultured Diploid and Tetraploid Codonopsis lanceolata

Effects of Different Medium Composition and Exogenous Hormones on Browning of Tree Peony (Paeonia suffruticosa Andr.) Callus in Tissue Cultu

Browning is one of the key factors that influenced the callus subculture of tree peony (Paeonia suffruticosa Andr.). Effects of medium composition and exogenous hormones: macro elements of Murashige and Skoog (MS salts) and iron salt (Fe2+), pH, agar and 6-benzylaminopurine (6-BA), 1-naphthaleneacetic acid (NAA) and kinetin (KT) on the callus browning of P. suffruticosa ‘Shan Hu Tai’ in vitro were studied in this paper. Results showed that the browning of P. suffruticosa callus were more sensitive to KT than 6-BA in different concentrations of 6-BA and KT separately with different concentrations of NAA, and reduced to the lowest (13.3%) under 0.5 mg·L-1 NAA plus 0.3 mg·L-1 KT. 1/4 × MS plus 1/4 × Fe2+ was the best basic medium in which the browning rate was only 18.2%. The browning rate of the callus was the lowest of 4.0% under pH 6.5 and the callus grew better in 7.0 g·L-1 agar than others. This study indicated that the best medium preventing P. suffruticosa callus in vitro from browning was: 1/4 × MS medium supplemented with 6.95 mg·L-1Fe2+, 0.3 mg·L-1 KT, 0.5 mg·L-1 NAA, 6.0 g·L-1 agar and 30 g·L-1 sucrose in pH 6.5.

Effect of media composition and light supply on biomass, lipid content and FAME profile for quality biofuel production from Scenedesmus abundans

Microalgae have a potential to serve as a renewable feedstock for synthesis of biodiesel, rich in saturated and unsaturated fatty acids and valuable biomolecules. Role of several parameters such as nutrient composition, light intensity, photoperiod and pH on growth and lipid productivity was investigated. A freshwater green microalga Scenedesmus abundans was selected as a potential candidate for production of biodiesel and its growth conditions were optimized for enhanced lipid accumulation. The lipid content of the strain has been increased up to about 48% when grown in modified Fogg’s medium at pH 8 with light intensity of 40.5μmolm−2s−1 and photoperiod of 16h light:8h dark. Lipids were transesterified and the fatty acid methyl esters (FAME) thus obtained were analyzed by GC–MS. They possess more than 80% of C16 to C18 fatty acids, which are major precursors for biodiesel production. The FAME consisted of saturated (47.83%), monounsaturated (26.38%) and polyunsaturated (25.20%) fatty acids. Several important biodiesel quality parameters were predicted and compared to the corresponding specifications in the American and European biodiesel standards. The study opens up new vistas for utilization of the microalga, S. abundans for efficient production of biodiesel.

Effect of culture medium on fermentative and CO-dependent H2 production activity in Citrobacter amalonaticus Y19

Citrobacter amalonaticus Y19 is a unique chemoheterotrophic microorganism capable of carbon monoxide (CO)-dependent hydrogen (H2) production. To improve its biocatalytic potential, the effects of medium composition on cell growth and H2 production were investigated. Among several carbon sources tested, maltose resulted in the best CO consumption and H2 production. Meanwhile, l-cysteine supplementation improved cell growth and H2 production, suggesting that this amino acid stimulates synthesis of FeS clusters. When molybdenum and selenium, which are essential cofactors of formate-hydrogen lyase (FHL), were added, formate accumulation was reduced. Optimized medium composition improved the activities of CO-dependent H2 production (6.3-fold), CODH (22.3-fold) and hydrogenase (2.9-fold). The optimized medium composition also enhanced H2 production in recombinant C. amalonaticus Y19-cooA, which homologously overexpresses CooA, the transcriptional activator of the coo operons. Cells grown under this condition exhibited improved stability in the course of repeated-batch CO-dependent H2 production under non-growing conditions.

Setting accelerated dissolution test for PLGA microspheres containing peptide, investigation of critical parameters affecting drug release rate and mechanism

The objective of this study was development of accelerated in vitro release method for peptide loaded PLGA microspheres using flow-through apparatus and assessment of the effect of dissolution parameters (pH, temperature, medium composition) on drug release rate and mechanism. Accelerated release conditions were set as pH 2 and 45°C, in phosphate buffer saline (PBS) 0.02M. When the pH was changed from 2 to 4, diffusion controlled phases (burst and lag) were not affected, while release rate during erosion phase decreased two-fold due to slower ester bonds hydrolyses. Decreasing temperature from 45°C to 40°C, release rate showed three-fold deceleration without significant change in release mechanism. Effect of medium composition on drug release was tested in PBS 0.01M (200 mOsm/kg) and PBS 0.01M with glucose (380 mOsm/kg). Buffer concentration significantly affected drug release rate and mechanism due to the change in osmotic pressure, while ionic strength did not have any effect on peptide release. Furthermore, dialysis sac and sample-and-separate techniques were used, in order to evaluate significance of dissolution technique choice on the release process. After fitting obtained data to different mathematical models, flow-through method was confirmed as the most appropriate for accelerated in vitro dissolution testing for a given formulation.

Medium factors on anaerobic production of rhamnolipids by Pseudomonas aeruginosa SG and a simplifying medium for in situ microbial enhanced oil recovery applications.

Aerobic production of rhamnolipid by Pseudomonas aeruginosa was extensively studied. But effect of medium composition on anaerobic production of rhamnolipid by P. aeruginosa was unknown. A simplifying medium facilitating anaerobic production of rhamnolipid is urgently needed for in situ microbial enhanced oil recovery (MEOR). Medium factors affecting anaerobic production of rhamnolipid were investigated using P. aeruginosa SG (Genbank accession number KJ995745). Medium composition for anaerobic production of rhamnolipid by P. aeruginosa is different from that for aerobic production of rhamnolipid. Both hydrophobic substrate and organic nitrogen inhibited rhamnolipid production under anaerobic conditions. Glycerol and nitrate were the best carbon and nitrogen source. The commonly used N limitation under aerobic conditions was not conducive to rhamnolipid production under anaerobic conditions because the initial cell growth demanded enough nitrate for anaerobic respiration. But rhamnolipid was also fast accumulated under nitrogen starvation conditions. Sufficient phosphate was needed for anaerobic production of rhamnolipid. SO4(2-) and Mg(2+) are required for anaerobic production of rhamnolipid. Results will contribute to isolation bacteria strains which can anaerobically produce rhamnolipid and medium optimization for anaerobic production of rhamnolipid. Based on medium optimization by response surface methodology and ions composition of reservoir formation water, a simplifying medium containing 70.3 g/l glycerol, 5.25 g/l NaNO3, 5.49 g/l KH2PO4, 6.9 g/l K2HPO4·3H2O and 0.40 g/l MgSO4 was designed. Using the simplifying medium, 630 mg/l of rhamnolipid was produced by SG, and the anaerobic culture emulsified crude oil to EI24 = 82.5 %. The simplifying medium was promising for in situ MEOR applications.

The Effect of Media Composition and Organic Fertilizer Concentration on the Growth and Yield of Red Ginger Rhizome (Zingiber officinale Rosc.)

Abstract The red ginger (Zingiber officinale. Rosc.) is an annual herbaceous plant of high economic value, with a harvest of 10-12 months. The export demand of red ginger rhizome has increased up to 20-22% for the last five years and it has not been able to be sufficed by the red ginger producers in Indonesia. This situation happens because of the very limited amount of red ginger production area, a still very conventional method of red ginger cultivation and the low quantity of organic substances in the soil. Other than that the use of expensive inorganic fertilizers often increases the production cost of red ginger, besides from being unsafe to both the environment and also the health of humans. Various technological breakthrough innovations have been done in order to meet the needs of red ginger export, one of which is the use of the polybag cultivation system which utilizes the organic waste as a media composition. The combination of organic substances with the application of liquid organic fertilizers was expected to increase and improve the growth and yield of red ginger rhizome. The research was conducted at Agrotechnopark experimental field Jember University from January 2013 until June 2014. The aim of field experiment was to find out the effect of media compositions and organic fertilizer concentrations on growth and yield of red ginger rhizome (Zingiber officinale. Rosc.). The field experiment factorials (3x4) used in randomized block designs with three replications. The first factor is the media compositions (bokashi: charcoal husk: coco peat) consisting of three levels: M1 (40%: 30%: 30%), M2 (50%:25%: 25%), and M3 (60%: 20%: 20%) and the second factor being the organic fertilizer concentration consisting of four levels: P1 (0 cc/l), P2(1.5 cc/l), P3 (3 cc/l), and P3 (4.5 cc/l). The results of this study concluded that a single factor of various composition media (M) was not significantly influence on all the parameters of growth and yield of red ginger rhizome. The single factor of concentrations of liquid organic fertilizer (P) and the interaction of two factors treatments (MP) was significantly influence on all parameters of the growth and yield of red ginger rhizome. The combination of treatments (M3P3) gave the best response to all parameters: plant height (58.8 cm), number of leaves (25.2), number of buds (35.6), rhizome fresh weight per plant (2329.64 g), total biomass (258.14 g), zingeron level (1.88%) and oleoresin level (1.57%).