Effect Of Different Substrates Research Articles

Seedling development of Melochia pyramidata in different substrates and pot sizes

Native plants such asMelochia pyramidatahave been targeted in various scientific studies due to their potential uses. From this perspective, the present study aimed to separately evaluate the effect of different substrates and pot sizeson the production of seedlings of M. pyramidatagenotypes propagated by cuttings. Theexperimentwas conducted in a plant nursery at the Center of Agricultural Sciences of theFederal University of Paraíba, Areia, PB, Brazil. Theexperimental design was completely randomizedandarranged in a 3 x 2factorialdesignwith three substrate types (sand, commercial substrate Plantmax®, andsand+ manure) and two genotypes(MP1 andMP2), withfive replications. Pot size evaluation used a 2 x 2factorial designwith two pot sizes(V1: 1.8 LandV2: 0.9 L), two genotypes(MP1 andMP2), andfour replications. The evaluations began seven days after the cuttings were planted in tubes and continued weekly for two months by evaluating the number of sprouts and leaves. After two months, these seedlings were transplanted to pots and evaluated for the number of sprouts, leaves, fruits, flower buds, leaf width, and leaf length. The data were subjected to analysis of variance and test of means(Tukey) at5% probability. The cuttings ofM. pyramidatadeveloped best in washed sand and in the commercial substratePlantmax®. The genotypes developed best in 1.8-L pots, showing more leaves, flower buds, and fruits.

Effects of different substrates on growth and development of Globe amaranth (Gomphrena globosa L.)

Commercial substrates have the greatest application in the production of ornamental flowers. Depending on the species being produced, there is an adequate substrate for each production. Thanks to a wide range of commercial substrates, they can be completely adapted to the type of plants, and provide everything they need in the most sensitive stage of life, as well as in the later stages. The research is based on the possibilities and effectiveness of different substrates on growth and development of roots and above ground parts of the Globe amaranth (Gomphrena globosa L.), in the seedling phase and in the adult plant phase. The research was conducted in the "Gornja Puharska" Nursery in Prijedor during 2019. The substrates used in the experiment are the mixture of garden soil, Baltic, and peat from Grahovo, Hawitta baltisches UBI 20 Tonsubstrat 2, and Klasmann-Deilmann - TS3 Medium basic substrate. Measurements of morphological parameters of growth and development of plants (the plant height, number of leaves and flowers, and flower diameter) were performed, as well as determination of weight of fresh and dry roots and above groundarts and the number of seeds. Effect of medium basic substrate is reflected in an increase in the average value of vegetative morphological parameters (the plant height and number of leaves) of Globe amaranth seedlings and adult plants. Furthermore, this substrate had a positive effect on both fresh and dry mass of adult plants, as well as the number of obtained seeds, compared to the other two substrates. Efficiency of substrate comprising a mixture of garden soil, Baltic, and peat from Grahovo, is reflected in an increase in the average values of generative morphological parameters (the number of flowers and flower diameter) of the Globe amaranth - Gomphrena globosa L., compared to commercial substrates applied.

The Effect of Different Substrates on Morphological Characteristics of Acclimatized Bowiea Volubilis

Before acclimatization of Bowiea volubilis, in vitro propagated bulbs were cleaned and separated into four sizes (from 6 to 15 mm). We formed a total of 10 groups with 30-30 individuals, distributing the sizes evenly. Four types of substrate (peat, perlite, coconut fiber, sand) and their mixtures of 50-50% were used. Previously we examined three parameters: length, diameter and weight of the bulbs; later the weight of the successfully acclimatized plants, the number and length of roots and leaves were measured. The acclimatization was done in one of the greenhouses of the Buda Arboretum, where the plants were grown with fleece covering till one month, irrigated every three days, without the use of nutrient replenishment and artificial lighting. After 3 month period, survived plants developed effectively on peat + perlite, sand + perlite and sand + peat mixtures. In these cases, we achieved the largest increases in roots, green parts, bulbs and total weight.

Substrate-dependent differential regulation of mitochondrial bioenergetics in the heart and kidney cortex and outer medulla

The kinetics and efficiency of mitochondrial oxidative phosphorylation (OxPhos) can depend on the choice of respiratory substrates. Furthermore, potential differences in this substrate dependency among different tissues are not well-understood. Here, we determined the effects of different substrates on the kinetics and efficiency of OxPhos in isolated mitochondria from the heart and kidney cortex and outer medulla (OM) of Sprague-Dawley rats. The substrates were pyruvate+malate, glutamate+malate, palmitoyl-carnitine+malate, alpha-ketoglutarate+malate, and succinate±rotenone at saturating concentrations. The kinetics of OxPhos were interrogated by measuring mitochondrial bioenergetics under different ADP perturbations. Results show that the kinetics and efficiency of OxPhos are highly dependent on the substrates used, and this dependency is distinctly different between heart and kidney. Heart mitochondria showed higher respiratory rates and OxPhos efficiencies for all substrates in comparison to kidney mitochondria. Cortex mitochondria respiratory rates were higher than OM mitochondria, but OM mitochondria OxPhos efficiencies were higher than cortex mitochondria. State 3 respiration was low in heart mitochondria with succinate but increased significantly in the presence of rotenone, unlike kidney mitochondria. Similar differences were observed in mitochondrial membrane potential. Differences in H2O2 emission in the presence of succinate±rotenone were observed in heart mitochondria and to a lesser extent in OM mitochondria, but not in cortex mitochondria. Bioenergetics and H2O2 emission data with succinate±rotenone indicate that oxaloacetate accumulation and reverse electron transfer may play a more prominent regulatory role in heart mitochondria than kidney mitochondria. These studies provide novel quantitative data demonstrating that the choice of respiratory substrates affects mitochondrial responses in a tissue-specific manner.

The effect of different substrates on chitinase activity from Bacillus sp. WS4F

One of the roles of chitinase is as an antifungal which is widely used as a biocontrol agent for plant diseases caused by pathogenic fungi. Bacillus sp. WS4F has chitinase activity which can inhibit the growth of Ganoderma boninense, a fungus that attacks oil palm and causes basal stem rot (BSR). This study aims to investigate the effect of different substrates on the activity of the chitinase from Bacillus sp. WS4F. Two kinds of substrates i.e. chitin flakes and shrimp shells were used in this study. Enzyme activity of chitinase was analyzed after partial purification of enzyme was performed using ammonium sulfate precipitation followed by dialysis. The highest activity of chitinase was achieved by the substrate using shrimp shells. The ammonium sulfate precipitation (60-80% saturation) 0.0949 U/mL for activity enzyme and 0.2639 mg/mL for protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the enzyme showed a molecular weight of 64.389 kDa.

The effect of different substrates on oyster mushroom (Pleurotus ostreatus) spawn growth

The study aimed to find alternative substrates for growing spawn of oyster mushroom (Pleurotus ostreatus). The experiment was laid out in a completely randomized design with three compositions of substrates. The substrate compositions were: (1) Substrate A (corn 100%), (2) Substrate B (corn and sawdust mix in a ratio of (1:1, v/v)), and (3) Substrate C (corn and sawdust mix in a ratio of (3:1, v/v)). All compositions were replicated 18 times. The complete colonization (days) and mycelium growth rate (cm day−1) were recorded. Means were analyzed by Analysis of Variance (ANOVA) and duncan test was performed if there were significant between the substrate compositions. The results showed that substrate C was the best substrate indicated with the fastest complete colonization of 14.17±0.92 and the highest growth rate of 0.85±0.06 cm day−1.

Substrates, emergence and initial development of passion fruit seedlings

The propagation of any crop is understood as a crucial step for the success of the crop, being the main form of propagation of passion fruit to sexual, where almost all cases of commercial plantations, orchards are formed from seedlings generated from seeds produced in substrates. The objective was then to evaluate the effect of different substrates on the emergence and initial development of passion fruit (Passiflora edulis) seedlings. The experimental design was a randomized block with 6 treatments and 4 repetitions with 25 seeds per experimental unit. The seeds were submitted to the treatments, sown in different substrates, being: T1 = Pure soil (100%); T2 = Soil (75%) + humus (25%); T3 = Soil (75%) + bovine manure (25%); T4 = Soil (75%) + chicken manure (25%); T5 = Soil (75%) + Natufert® organomineral fertilizer (25%); T6 = Soil (75%) + coffee straw (25%). Thirty days after sowing the variables were evaluated, percentage of germination. Sixty days after sowing were evaluated: number of leaves; plant height; collection diameter; root length; leaf green mass; leaf dry mass; green root mass; dry root mass. The treatment Terra (75%) + coffee straw (25%) proved to be the most recommended with regard to variables of the aerial part, while Terra (75%) + humus (25%) and Terra (75%) + bovine manure (25%) were the most suitable for the root development of seedlings.

Pertumbuhan Trepang Holothuria scabra yang dipelihara dalam kurungan dengan substrat berbeda di Perairan Pantai Bahoi

This research was conducted to examine the effect of different substrates on the growth of Sea Cucumber Holothuria scabra reared in cages in Bahoi Coastal waters. This research was conducted from April to June 2021 in Bahoi Coastal Waters, West Likupang, North Minahasa. The method used in this research is the observation method with three treatments: A (sand), B (mud), C (seagrass), and three replications. The cages are made of nets size 100 cm x 100 cm x 50 cm as many as 9 cages. The sea cucumber size data was collected every two weeks. Samples of sea cucumber were taken randomly from each cage, left for ± 10 minutes, photographed, weighed, then recorded. Data collection for water quality is carried out every week during the day at the lowest tide, between 12:00 to 14:00. Water quality parameters were measured using a thermometer, refractometer, and litmus paper. Based on the research results, the average absolute weight growth and daily growth rate of sea cucumber reared with mud substrate showed the best results, that is 0.55 g and 0.72%, while the sand and seagrass substrates showed negative values. The results of ANOVA (analysis of variance) showed that there was no significant difference between absolute growth and daily growth rate of Sea Cucumber H. scabra reared on sand, mud, and seagrass substrates.

Enhanced Phytase Production by Bacillus subtilis subsp. subtilis in Solid State Fermentation and its Utility in Improving Food Nutrition

Phytic acid acts as anti-nutritional factor in food and feed ingredients for monogastric animals as they lack phytases. Phytase production by Bacillus subtilis subsp. subtilis JJBS250 was studied in solid-state fermentation and its applicability in dephytinization of food. Bacterial culture was grown in solid state fermentation using wheat bran and various culture conditions were optimized using 'One variable at a time' (OVAT) approach. Effects of different substrates (wheat bran, wheat straw, sugarcane bagasse), incubation time (24, 48, 72 and 96 h), incubation temperatures (25, 30, 35 and 40°C), pH (4.0, 5.0, 6.0, 7.0 and 8.0) and moisture content (1:1.5, 1:2.0, 1:2.5 and 1:3) were studied on phytase production. Bacterial phytase was used in dephytinization of food samples. Optimization of phytase production was studied in solid state fermentation (SSF) using 'One variable at a time' (OVAT) approach. Bacillus subtilis subsp. subtilis JJBS250 grew well in various agroresidues in SSF and secreted high enzyme titres using wheat bran at 30°C and pH 5.0 after incubation time of 48 h with substrate to moisture ratio of 1:3. Glucose and ammonium sulphate supplementation to wheat bran further enhanced phytase production in SSF. Optimization of phytase production resulted in 2.4-fold improvement in phytase production in solid state fermentation. The enzyme resulted in dephytinization of wheat and rice flours with concomitant release of inorganic phosphate, reducing sugar and soluble protein. Optimization resulted in 2.34-fold enhancement in phytase production by bacterial culture that showed dephytinization of food ingredients with concomitant release of nutritional components. Therefore, phytase of B. subtilis subsp. subtilis JJBS250 could find application in improving nutritional quality of food and feed of monogastric animals.

Effects of Different Substrates on the Formability and Densification Behaviors of Cemented Carbide Processed by Laser Powder Bed Fusion

Cemented carbide materials are widely applied in cutting tools, drill tools, and mold fabrication due to their superior hardness and wear resistance. Producing cemented carbide parts via the laser powder bed fusion (L-PBF) method has the advantage of fabricating complex structures with a rapid manufacturing speed; however, they were underdeveloped due to their low density and crack formation on the blocks. This work studied the effect of different substrates including 316L substrates, Ni200 substrates, and YG15 substrates on the forming quality of WC-17Co parts fabricated by L-PBF, with the aim of finding the optimal substrate for fabrication. The results revealed that the Ni200 substrates had a better wettability for the single tracks formation than other substrates, and bonding between the built block and the Ni200 substrate was firm without separation during processing with a large range of laser energy inputs. This guaranteed the fabrication of a relatively dense block with fewer cracks. Although the high laser energy input that led to fine crack formation on the blocks formed on the Ni200 substrate, it was found to be better suited to restricting cracks than other substrates.

Uso de lodo de esgoto na produção e na qualidade de mudas e teores de nutrientes em Hovenia dulcis (Rhamnaceae)

The objective of this study was to evaluate the effect of different substrates containing sewage sludge on the quality and content of macro and micronutrients of Hovenia dulcis Thunberg. The treatments were composed of different substrate compositions containing sludge and sewage, and biometric characteristics were measured and macro and micronutrient contents were determined. It was observed that the increasing order of the total macronutrient content evaluated in the root was K > N > Mg > Ca > P > S and at the aerial part> Ca > N > K > P > Mg > S; for the micronutrients, at the root content was Fe > Zn > Mn > B > Cu and in the aerial part Fe > Mn > Zn > B > Cu. The use of sewage sludge as substrate provided better nutritional quality seedlings with better growth and increase of aerial and root biomass.

Effect of Different Substrates on Growth and Nutrient Absorption of Cinnamomum chekiangense Seedlings

Cinnamomum chekiangense is an important and precious tree species. In vigorously promoting precious tree species for the afforestation, the cultivation of high-quality container seedlings is the key to the success of afforestation, and the seedling substrate directly determines the quality of seedlings. In this paper, 1-year-old seedlings of C. chekiangense were selected as the research object, using potted culture method to study the effects of 5 substrates (yellow soil, sawdust, peat) on seedling plant height, ground diameter, biomass growth and accumulation of nitrogen, phosphorus and potassium, in order to screen out the suitable substrate ratio for the cultivation of the container seedlings of the C. chekiangense and provide the basis for the cultivation of high-quality container seedlings. The results showed that A3 (yellow soil∶peat∶sawdust=40∶30∶30) substrate was most conducive to the growth of C. chekiangense, and the plant height and ground diameter were significantly higher than A5 (P<0.05). The leaves, branches, roots and total biomass of C. chekiangense cultivated with A3 substrate were largest, which were 33.63, 39.57, 48.43, 121.64 g·plant−1, respectively; significantly higher than other treatments (P<0.05). The accumulation of nitrogen, phosphorus, and potassium in the whole plant of C. chekiangense cultivated with A3 substrate were the best, which were 3168.8, 137.3, 673.1 mg·plant−1 respectively; Gray correlation analysis method evaluation showed that A3 substrate was the best substrate for the growth of the C. chekiangense container seedlings.

Effect of substrate on operation performance of ecological floating bed for treating simulated tailwater from wastewater treatment plant

ABSTRACT Ecological floating bed (EFB) with assembling substrate can effectively enhance the removal of nitrogen. However, the influence of the substrate on the operation process of the EFB remains unclear. In this study, EFBs with zeolite, sponge iron and complementary substrates (zeolite and sponge iron) were established to explore the effects of different substrates on nitrogen removal efficiency, physical and chemical properties and plant growth. Complementary substrates could efficiently and stably remove nitrogen from tailwater. The concentration of ammonium nitrogen (NH4 +-N), nitrate nitrogen (NO3 --N) and total nitrogen (TN) in the effluent during the operation were lower than 1, 1 and 1.5 mg/L, respectively. Additionally, complementary substrates had no inhibitory effect on plant growth, and those substrates had little influence on the distribution of dissolved oxygen (DO), the oxidation–reduction potential (ORP) and the pH value. The zeolite increased the NH4 +-N removal and promoted the growth of plant stems and roots. However, sponge iron could effectively remove the NO3 --N in the water body and inhibit the growth of plants. These results can provide a basis for the EFB in the reuse of the tailwater of wastewater treatment plant.

Water‐assisted mechanical testing of polymeric thin‐films

AbstractThin films with a nanometer‐scale thickness are of great interest to both scientific and industrial communities due to their numerous applications and unique behaviors different from the bulk. However, the understanding of thin‐film mechanics is still greatly hampered due to their intrinsic fragility and the lack of commercially available experimental instruments. In this review, we first discuss the progression of thin‐film mechanical testing methods based on the supporting substrate: film‐on‐solid substrate method, film‐on‐water tensile tests, and water‐assisted free‐standing tensile tests. By comparing past studies on a model polymer, polystyrene, the effect of different substrates and confinement effect on the thin‐film mechanics is evaluated. These techniques have generated fruitful scientific knowledge in the field of organic semiconductors for the understanding of structure–mechanical property relationships. We end this review by providing our perspective for their bright prospects in much broader applications and materials of interest.

Enhanced Photo–Fenton Removal Efficiency with Core-Shell Magnetic Resin Catalyst for Textile Dyeing Wastewater Treatment

Heterogeneous photo–Fenton reactions have been regarded as important technologies for the treatment of textile dyeing wastewaters. In this work, an efficient core-shell magnetic anion exchange resin (MAER) was prepared through in situ polymerization and used to remove reactive brilliant red (X-3B) in a UV–Fenton system. The MAER exhibited satisfactory removal efficiency for X-3B because of its highly effective catalytic activity. More than 99% of the X-3B (50 mg/L) was removed within 20 min in the UV–Fenton reaction. This is because the uniformly dispersed core-shell magnetic microsphere resin could suppress the aggregation of Fe3O4 nanoparticles and, thus, enhance the exposure of Fe reaction sites for catalytic reaction with H2O2. The good adsorption capacity of MAER also played an important role in promoting contact between X-3B and reactive radicals during the reaction. Mechanism research showed that hydroxyl radical (•OH) was the main reactive radicals for the removal of X-3B in the MAER UV–Fenton system. The MAER can be easily separated by a magnet after catalytic reactions. Moreover, the matrix effects of different substrates (Cl−, NO3−, SO42−, and humic acid) were investigated. The results showed that SO42− could be beneficial to improve the removal of X-3B but that the others decrease the removal. The MAER UV–Fenton also removed significant amounts of total organic carbon (TOC) for the X-3B solution and an actual textile dyeing industrial wastewater. The heterogeneous oxidation system established in this work may suggest prospects for practical applications in the treatment of textile dyeing wastewater.

Optimization of Substrate and Nutrient Solution Strength for Lettuce and Chinese Cabbage Seedling Production in the Semi-Arid Environment of Central Myanmar

The growing population of tropical countries has led to a new awareness of the importance of vegetables as a source of essential foods and nutrients. The success of vegetable cultivation depends to a large extent on high-quality seedlings. This work aimed at evaluating the effects of different substrates and different nutrient solution concentrations on the development of lettuce and Chinese cabbage seedlings in a semi-arid tropical area. Three independent experiments were conducted at the Soil and Water Research Station at Yezin Agriculture University, Myanmar (Myanmar, 19.83° N; 96.27° E). In all experiments a randomized block design was implemented with four treatments and three repetitions. In the first experiment the adaptability of lettuce seedling to two substrates (namely a Hulls Manure mix composed by 50% of mature cattle manure and 50% of carbonized rice husk and a soil based substrate constituted by 70% local soil, 20% burned rice husk, and 10% fresh cattle manure) and two nutrient solutions with different electrical conductivities (ECs) (W0.1, stored rainwater with EC = 0.13 dS m−1 and NS1.2, nutrient solution with EC = 1.20 dS m−1) were tested. In the second and third experiments, two species (lettuce and Chinese cabbage) were assessed for their response to nutrient solution concentrations. In both crops, 4 fertigation treatments (W0.1; NS0.6; NS1.2; and NS1.8) were supplied, by modulating the concentration of a compound mineral fertilizer (15:15:15) in the following ranges: W0.1: 0 g L−1, electrical conductivity (EC) 0.13 dS m−1, NS0.6: 0.3 g L−1, EC of 0.60 dS m−1; NS1.2: 0.6 g L−1, 1.2 dS m−1 EC, and NS1.8: 0.9 g L−1, 1.8 dS m−1 EC. Adopting different substrates and applying different nutrient solutions significantly affected growth (fresh weight and leaf morphology) and some physiological parameters (stomatal conductance, leaf temperature, and leaf chlorophyll content) of lettuce and Chinese cabbage seedling. From the first experiment, the combination of the soil based substrate and NS1.2 treatments allowed us to improve the seedlings’ growth. In the second experiment, highest growth of lettuce and Chinese cabbage seedlings was associated with NS1.2 and NS1.8, respectively. The presented results allow for the optimization of both growing media and nutrient solution management when lettuce and Chinese cabbage seedling are produced in the Central Dry Zone of Myanmar.

Comparison of water nanodroplet properties on different graphite-based substrates

The molecular structure and dynamics of water differ considerably at various interfaces. We compare the interfacial water structure–property relationship on three different carbon substrates, namely, amorphous carbon, compressed expanded natural graphite, and pure graphite by utilizing atomistic molecular dynamics simulations. The effect of different substrates on the structural and dynamical properties of water can readily be observed. The density distributions parallel and normal to the substrates show oblate droplet structures. The normal to the substrate water distribution shows a strong hydration layer at the interface that does not vary with substrates. However, the disparity in the structure and dynamics on three different substrates shows that the surface morphologies of the substrates are critical for determining nanoscale water properties. Furthermore, it is observed that the formation of an interfacial water layer or the hydration layer is a direct consequence of both water “confinement” at the nanoscale and “attraction” between water molecules and the carbon substrates.

The effects of different substrates on the nutritional composition of oyster mushrooms (Pleurotus sajor-caju)

Oyster mushroom were cultivated in controlled environment using different agricultural and forest waste. Experiment were conducted using six treatments viz. S1 (Red gram straw) S2 (Wheat Straw) S3 (Fallen leaf of Gum arabic tree + Custard apple+ Teak), S4 (Rice bran) S5, (Soybean straw) and S6, (Fallen leaf of Palash + Teak). The average yield of Mushroom produced on each bed was noted. It is observed that the dry weight of mushroom cultivated on substrate S6 (Fallen leaf of Palash + Teak) was found more followed by mushroom cultivated on treatment S1 (Red gram straw) (76gm) than the rest of the treatments. Biochemical study revealed that moisture percent was found highest in mushroom cultivated on paddy straw (35.83%) whereas lowest moisture percent was noted in mushroom grown on (S6) fallen leaf of Palash and Teak (31.68%). The crude fiber percent was found highest (18.63%) in mushroom grown on treatment S2 (Wheat straw) and was noted low (8.76%) in treatment S1 (Red gram straw) mushrooms. The ash percent was highest in mushroom grown on treatment S6 (10.66%) and lowest in mushroom obtained on treatment S5 (5.38%). The highest protein content was measured in mushroom produced on treatment S4 (12.24 mg/ml) and was found lowest for treatment S2 (10.28 mg/ml) The protein content was varied in mushroom grown on different treatment and was found in the range of (12.20 – 10.35 mg/ml). This study revealed that the oyster mushrooms grown on different straw were differ in nutritional composition although belongs to same genus.

Measurement of Effects of Different Substrates on the Mechanical Properties of Submicron Titanium Nickel Shape Memory Alloy Thin Film Using the Bulge Test.

This study investigated the effects of different substrates on the mechanical properties of Ti-60at%Ni shape memory alloys (SMA). Two types of samples were prepared for this experiment: (1) a Ti-60at%Ni deposited on SiNx, and (2) a Ti-60at%Ni deposited on SiNx/Cr; both had a 600 nm thick film of Ti-60at%Ni. Deposition was done using the physical vapor deposition (PVD) process, and the microstructural changes and crystallization phase changes were observed through scanning electron microscopy (SEM) and X-ray diffraction (XRD). The results showed that the TiNi thin film with a Cr adhesion layer had better mechanical properties. The bulge test showed that TiNi thin film with a Cr adhesion had a higher Young’s modulus and lower residual stress. From the thermal cycling experiment, it was found that the Cr adhesion layer buffered the mismatch between TiNi and SiNx. Additionally, the thermal cycling test was also used to measure the thermal expansion coefficient of the films, and the fatigue test showed that the Cr layer significantly improved the fatigue resistance of the TiNi film.

α-Amylase and cellulase production by novel halotolerant Bacillus sp.PM06 isolated from sugarcane pressmud.

A novel Bacillus sp.PM06 isolated from sugarcane waste pressmud was tested for extracellular α-amylase and cellulase enzyme production. The effect of different substrates, nitrogen sources, pH, and temperature on growth and extracellular enzyme production was examined. Bacillus sp.PM06 was able to grow with starch and carboxymethyl cellulose (CMC) as a sole source of carbon and ammonium chloride was found to be the best nitrogen source. Maximum enzyme production was obtained at 48H for both α-amylase and cellulase. The optimal condition for measuring enzyme activity was found to be pH 5.5 at 50°C for α-amylase and pH 6.4 at 60°C for cellulase respectively. It was found that Bacillus sp.PM06 exhibited halotolerance up to 2M Sodium chloride (NaCl) and Potassium chloride (KCl). The isolate could produce α-amylase in the presence of 2M NaCl and 1M KCl. However, the strain produced cellulase even in the presence of 2M NaCl and KCl. Concomitant production of both enzymes was observed when the medium was supplemented with starch and CMC. A maximum of 31±1.15U/mL of amylase and 15±1.5U/mL of cellulase was produced in 48H. The enzyme was partially purified by Ammonium sulphate (NH4 )2 SO4 precipitation with 2.2 and 2.3-fold purification.