Abstract
A novel Bacillus sp.PM06 isolated from sugarcane waste pressmud was tested for extracellular α-amylase and cellulase enzyme production. The effect of different substrates, nitrogen sources, pH, and temperature on growth and extracellular enzyme production was examined. Bacillus sp.PM06 was able to grow with starch and carboxymethyl cellulose (CMC) as a sole source of carbon and ammonium chloride was found to be the best nitrogen source. Maximum enzyme production was obtained at 48H for both α-amylase and cellulase. The optimal condition for measuring enzyme activity was found to be pH 5.5 at 50°C for α-amylase and pH 6.4 at 60°C for cellulase respectively. It was found that Bacillus sp.PM06 exhibited halotolerance up to 2M Sodium chloride (NaCl) and Potassium chloride (KCl). The isolate could produce α-amylase in the presence of 2M NaCl and 1M KCl. However, the strain produced cellulase even in the presence of 2M NaCl and KCl. Concomitant production of both enzymes was observed when the medium was supplemented with starch and CMC. A maximum of 31±1.15U/mL of amylase and 15±1.5U/mL of cellulase was produced in 48H. The enzyme was partially purified by Ammonium sulphate (NH4 )2 SO4 precipitation with 2.2 and 2.3-fold purification.
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