Effects Of Collagen Peptides Research Articles

Effects of Collagen Peptides from Cod (Gadus) Skin on Delaying H2O2-Induced Senescence of 2BS Cells

ABSTRACT The purpose of this research was to explore the impact of collagen peptides derived from cod (Gadus) skin on H2O2-induced 2BS senescence cells. The senescence model induced by H2O2 was established to determine the cell proliferation rate, ROS level, cell apoptosis, and peroxidase content after the intervention of the optimal dose of collagen peptides. Collagen peptides effectively suppressed apoptosis in 2BS senescence cells, substantially decreasing ROS and MDA levels. Furthermore, SOD and CAT activities were significantly elevated. In summary, collagen peptides have demonstrated a significant capacity to impede the process of cellular senescence in 2BS cells through the reduction of oxidative stress and apoptosis.

573 Marine and Bovine-Derived Collagen Peptides Inhibit Catagen Development and Generate Diverse Effects on Stem Cells and Progeny in Human Scalp Hair Follicles Ex Vivo

Hair loss and thinning result from premature catagen and telogen development, and terminal to vellus conversion, the latter caused also by reduced bulge hair follicle stem cell (HFSC) number or ability to produce cell progeny. Although it is known that nutraceutical supplementation with collagen peptides can improve skin physiology, and that loss of type XVII collagen leads to the depletion of HFSCs in mice, the effect of collagen peptides on HF biology remains elusive. Thus, we investigated the effect of bovine (Peptan®B) and fish (Peptan®Marine)-derived collagen peptides on HF biology.

Targeting inflammation with collagen.

Tissue damage caused by an infection oran autoimmune disease triggers degradation of collagen in the extracellular matrix (ECM), which further enhances inflammation. Therefore, improving ECM in aninflamed tissue can be exploited as a potential therapeutic target. A recentstudy emphasised an innovative approach against COVID‐19 using polymerised type I collagen (PTIC) that improves disease severity through a hitherto unknownmechanism. In this paper, we provide an overview of potential mechanism thatmay explain the anti‐inflammatory effect of collagen peptides. In addition,the paper includes a brief summary of possible side effect of collagendeposition in inflammatory diseases. Altogether, current knowledge suggeststhat collagen may potentially reduce the residual risk in inflammatorydiseases; however, the detailed mechanism remains elusive.

Oral intake of Bioactive Collagen Peptides in the improvement of skin and hair: clinical studies by instrumental measurements

The effect of collagen peptides on various aspects of skin and hair physiology is known but needs further studies. Thus, the objective of this study was to evaluate the clinical changes in skin and hair after 90 days of treatment with oral supplementation of 5 g/day of collagen peptides. For this, 60 healthy female participants were enrolled, aged 45 to 60 years old, with the primary objective of evaluating the effect of the ingestion of a bioactive collagen peptides-based supplementation in the cutaneous microrelief, reduction of wrinkles, thickness, and echogenicity of the dermis, as well as in the mechanical properties of the hair using biophysical and skin imaging techniques. The present study showed important benefits in relevant skin visual parameters, dermis density, and hair strength with the obtained data. In addition, considering that the aging process affects the hair's mechanical resistance due to hair fiber thinner, the proposed treatment was effective for aged hair. In conclusion, the collagen peptides oral supplementation is essential not only for improving skin conditions but also for hair care once it significantly increases the mechanical hair resistance evaluated by objective measurements. Keywords: Collagen peptides, skin aging, skin imaging techniques, hair mechanical properties, clinical study

Study on the Mechanism of the Blood-Glucose-Lowering Effect of Collagen Peptides from Sturgeon By-Products

In a previous study, we found that the collagen peptides prepared from the by-products of Bester sturgeon had an inhibitory effect on elevated blood glucose levels in a glucose tolerance test with ICR mice. In the present study, we examine the mechanism of the effect of sturgeon collagen peptides (SCPs) in detail. When glucose was orally administered to mice along with the SCPs, it was found that the glucose remained in the stomach for a longer time. In the above tests, the amount of glucose excreted in the feces of mice also increased. On the contrary, it was revealed that the SCPs have a dipeptidyl-peptidase-IV (DPP-IV) inhibitory ability in an in vitro test. In subsequent oral and intravenous glucose administration tests, glucagon-like peptide-1 (GLP-1) and insulin levels in the blood of mice were maintained at high levels. These results suggested the following three mechanisms: SCPs slow the rate of transportation of glucose from the stomach into the small intestine, resulting in delayed glucose absorption; SCPs suppress the absorption of glucose in the small intestine and excrete it from the body; SCPs inhibit DPP-IV in the blood and maintain a high GLP-1 level in blood, which in turn stimulates insulin secretion.

Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody

Active collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism.

Effects of Collagen Peptides on Recovery Following Eccentric Exercise in Resistance-Trained Males-A Pilot Study.

The authors sought to determine whether consuming collagen peptides (CP) enhances musculoskeletal recovery of connective tissues following a damaging exercise bout. Resistance-trained males consumed 15g/day of CP (n = 7) or placebo (n = 8), and after 7days, maximal voluntary isometric contraction (MVIC), countermovement jump height, soreness, and collagen turnover were examined. Five sets of 20 drop jumps were performed and outcome measures were collected 24, 48, and 120hr postexercise. Countermovement jump height was maintained in the CP group at 24hr (PRE = 39.9 ± 8.8cm vs. 24hr = 37.9 ± 8.9cm, p = .102), whereas the CP group experienced a significant decline at 24hr (PRE = 40.4 ± 7.9cm vs. 24hr = 35.5 ± 6.4cm, p = .001; d = 0.32). In both groups, muscle soreness was significantly higher than PRE at 24hr (p = .001) and 48hr (p = .018) but not at 120hr (p > .05). MVIC in both legs showed a significant time effect (left: p = .007; right: p = .010) over the 5-day postexercise period. Neither collagen biomarker changed significantly at any time point. CP supplementation attenuated performance decline 24hr following muscle damage. Acute consumption of CP may provide a performance benefit the day following a bout of damaging exercise in resistance-trained males.

Effect of Collagen Peptide, Alone and in Combination with Calcium Citrate, on Bone Loss in Tail-Suspended Rats.

Oral administration of bovine collagen peptide (CP) combined with calcium citrate (CC) has been found to inhibit bone loss in ovariectomized rats. However, the protective effects of CP and CP–CC against bone loss have not been investigated in a tail-suspension simulated microgravity (SMG) rat model. Adult Sprague-Dawley rats (n = 40) were randomly divided into five groups (n = 8): a control group with normal gravity, a SMG control group, and three SMG groups that underwent once-daily gastric gavage with CP (750 mg/kg body weight), CC (75 mg/kg body weight) or CP–CC (750 and 75 mg/kg body weight, respectively) for 28 days. After sacrifice, the femurs were analyzed by dual-energy X-ray absorptiometry, three-point bending mechanical tests, microcomputed tomography, and serum bone metabolic markers. Neither CP nor CP–CC treatment significantly inhibited bone loss in SMG rats, as assessed by dual-energy X-ray absorptiometry and three-point bending mechanical tests. However, both CP and CP–CC treatment were associated with partial prevention of the hind limb unloading-induced deterioration of bone microarchitecture, as demonstrated by improvements in trabecular number and trabecular separation. CP–CC treatment increased serum osteocalcin levels. Dietary supplementation with CP or CP–CC may represent an adjunct strategy to reduce the risk of fracture in astronauts.

Identification and Structure-Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin.

The effect of collagen peptides (CPs) in intestinal mucosal protection has been approved in both cell and animal models. However, its structure–activity relationship and efficient peptide sequences are unclear, which hinders the in-depth study of its action mechanism and relative nutraceuticals and pharmaceuticals development. In this work, size exclusion chromatography, cation-exchange chromatography, and RP-HPLC were used to separate Alaska pollock skin-derived collagen hydrolysates based on their molecular weight, charge property, and hydrophobicity. The intestinal epithelial barrier function (IEBF) protective effect of separated peptide fractions were evaluated by tumor necrosis factor (TNF)-α-induced Caco-2 cell model. Results indicated that lower molecular weight (500–1000 Da) and higher hydrophilicity of CPs were related to better IEBF protective effect. Two high-efficiency IEBF protective peptide sequences, GPSGPQGSR and GPSGLLGPK with the corresponding molecular weights of 841.41 Da and 824.38 Da, were subsequently identified by UPLC-QToF-MS/MS. Their IEBF protective ability are comparable or even better than the currently used intestinal health supplements glutamine and arginine. The present findings suggested that the hydrophilic CPs, with molecular weight between 500 Da to 1000 Da, should be preferred in IEBF protective peptides preparation. GPSGPQGSR and GPSGLLGPK might have the potential of being IEBF protective ingredients used in intestinal health supplements and drugs.

Collagen peptides administration in early enteral nutrition intervention attenuates burn-induced intestinal barrier disruption: Effects on tight junction structure

Abstract The effect of collagen peptides (CPs) administration in early basic enteral nutrition (EN) intervention after burn injury on intestinal barrier function was investigated in mice model. Burn-induced increases of intestinal permeability and disruption of intestinal barrier integrity were significantly attenuated by CPs supplemented EN at post-burn day 1, 3 and 7 as compared with only basic EN fed group. CPs revealed a prominent amelioration of the expression and localization of tight junction protein ZO-1 and occludin in intestinal mucosa. Furthermore, CPs administration significantly inhibited burn-induced elevation of MLCK expression and MLC phosphorylation. Simultaneously, the activation of involved signal pathways, such as NF-κB p65, MAPK p38, ERK and JNK were diminished in different extent by CPs addition. Therefore, CPs could be considered as a beneficial adjunct in early EN intervention to prevent intestinal barrier disruption following burn injury, because of its protective effects on intestinal tight junction integrity.

The effects of collagen peptides on muscle damage, inflammation and bone turnover following exercise: a randomized, controlled trial

This study examined whether consuming collagen peptides (CP) before and after strenuous exercise alters markers of muscle damage, inflammation and bone turnover. Using a double-blind, independent group’s design, 24 recreationally active males consumed either 20 g day−1 of CP or a placebo control (CON) for 7 days before and 2 days after performing 150 drop jumps. Maximal isometric voluntary contractions, countermovement jumps (CMJ), muscle soreness (200 mm visual analogue scale), pressure pain threshold, Brief Assessment of Mood Adapted (BAM +) and a range of blood markers associated with muscle damage, inflammation and bone turnover C-terminal telopeptide of type 1 collagen (β-CTX) and N-terminal propeptides of type 1 pro-collagen (P1NP) were measured before supplementation (baseline; BL), pre, post, 1.5, 24 and 48 h post-exercise. Muscle soreness was not significantly different in CP and CON (P = 0.071) but a large effect size was evident at 48 h post-exercise, indicative of lower soreness in the CP group (90.42 ± 45.33 mm vs. CON 125.67 ± 36.50 mm; ES = 2.64). CMJ height recovered quicker with CP than CON at 48 h (P = 0.050; CP 89.96 ± 12.85 vs. CON 78.67 ± 14.41% of baseline values; ES = 0.55). There were no statistically significant effects for the other dependent variables (P > 0.05). β-CTX and P1NP were unaffected by CP supplementation (P > 0.05). In conclusion, CP had moderate benefits for the recovery of CMJ and muscle soreness but had no influence on inflammation and bone collagen synthesis.

金枪鱼骨胶原蛋白肽螯合钙对去卵巢大鼠骨质疏松症的影响

金枪鱼骨胶原蛋白肽螯合钙对去卵巢大鼠骨质疏松症的影响

Ingestion of collagen peptides prevents bone loss and improves bone microarchitecture in chronologically aged mice

There is no knowledge about the effect of collagen peptides (CPs) on age-related bone loss. The purpose of this study was to examine whether CPs prevents bone loss in chronologically aged process. 13-month-old Kunming mice were administered with CPs for 8 weeks. Results showed that the bone mechanical strength, bone mineral density (BMD) and collagen content were significantly increased and the bone microarchitecture was greatly improved in the aged mice after CPs treatment. Further analysis of molecular mechanism showed that CPs upregulated collagen synthesis-related TGF-β and Smad3 level, but downregulated Smad7. Besides, serum level of tartrate resistant acid phosphatase (TRAP) was significantly decreased after CPs intake, whereas alkaline phosphatase (ALP) was not affected. These results indicate that CPs ingestion prevents age-related bone loss and improves bone microarchitecture by increasing collagen synthesis and inhibiting bone absorption and highlight its potential application as functional food to prevent osteoporosis in chronologically aged process.

Effects of oral administration of peptides with low molecular weight from Alaska Pollock (Theragra chalcogramma) on cutaneous wound healing

Protein supplements play an important role in wound healing. The effect of collagen peptide (PCP) and flesh protein peptide (FPP) from Alaska Pollock on wound healing was investigated. The molecular weight distributions of PCP and FPP were in a range of 430–1000 Da and 100–1000 Da, respectively. Compared to vehicle group, the wound healing activities in PCP and FPP were significantly enhanced, which could be corroborated by the increase of wound healing rate, hydroxylproline content, and tensile strength in rat wound model (P < 0.05). H&E showed a near-normal epidermis structure of PCP and FPP on day 12, but vehicle group displayed poor re-epithelialization. PCP and FPP increased the levels of bFGF, EGF, TGF-β1 and TβRII (P < 0.05), and decreased the content of Smad7 in TGF/Smad signaling pathway. Therefore, FPP and PCP can be used as effective ingredients in the treatment of wound healing.

Effect of collagen peptide in 13 old dogs which had gait disturbance

Effect of collagen peptide in 13 old dogs which had gait disturbance

Collagen Peptides from Crucian Skin Improve Calcium Bioavailability and Structural Characterization by HPLC-ESI-MS/MS.

The effects of collagen peptides (CPs), which are derived from crucian skin, were investigated in a retinoic acid-induced bone loss model. The level of serum bone alkaline phosphatase (BALP) in the model group (117.65 ± 4.66 units/L) was significantly higher than those of the other three groups (P < 0.05). After treatment with 600 and 1200 mg of CPs/kg, the level of BALP decreased to 85.26 ± 7.35 and 97.03 ± 7.21 units/L, respectively. After treatment with 600 mg of CPs/kg, the bone calcium content significantly increased by 22% (femur) and 12.38% (tibia) compared to those of the model group. In addition, the bone mineral density in the 600 mg of CPs/kg group was significantly higher (femur, 0.37 ± 0.02 g/cm2; tibia, 0.33 ± 0.02 g/cm2) than in the model group (femur, 0.26 ± 0.01 g/cm2; tibia, 0.23 ± 0.02 g/cm2). The morphology results indicated bone structure improved after the treatment with CPs. Structural characterization demonstrated that Glu, Lys, and Arg play important roles in binding calcium and promoting calcium uptake. Our results indicated that CPs could promote calcium uptake and regulate bone formation.

Integrative Gynecology and Women's Healthcare.

Complementary treatment approaches have been increasingly demanded by women during the last several decades to relieve their gynecological disorders, reduce side effects of conventional hormonal or cancer treatment, and enhance health-related quality of life. As many women do not disclose their complementary therapies to their gynecologists or oncologists, treatment plans are often not coordinated. Thus, the risk of adverse events and interactions of complementary with conventional therapies may be increased. Sharing of information between providers of conventional and complementary treatments is important to optimize health outcomes and safety. Integrative gynecology that combines conventional treatment with evidence-based complementary therapies and mind/body medicine has become a growing field of healthcare that strives to serve this demand. In integrative gynecology, conventional and complementary approaches are joined in a way that aims to optimize outcomes and prevent adverse events. The papers selected for this special issue represent a good panel for addressing this challenge. Additionally, the selected topics and papers represent the rich and many-facet knowledge, which we have the pleasure of sharing with the readers. Because of limited space, we can allow only a part of gynecological studies with high quality to be published in this special issue. More research and publication space are definitely needed for integrative gynecology in the future. The special issue contains ten papers. Two of them are related to bone loss. One paper introduces a screening system to identify Chinese herbs or formulae which will increase either HER2 or ERα protein expression of MCF-7 human breast cancer cell. Three papers are related to breast cancer risk of commonly used Chinese herbs; one of them considers the breast cancer risk of Chinese herbal remedies among hormone users, while another covers the subsequent endometrial cancer problems when tamoxifen users consume Ginseng. One paper focuses on the ovarian cancer risk of the herb-drug interaction between fermented wheat germ extract and cisplatin or docetaxel. One paper provides precious observational result of the effects of passive hydrotherapy among women in their ≥34th week of gestation. One paper tries to disclose the possible mechanism of Chinese herb for treating endometriosis. Finally, one paper introduces a practical method for studying the effectiveness and safety of traditional Chinese remedies for women with dysmenorrhea. In a paper titled “Bian Zheng Lun Zhi as a Complementary and Alternative Treatment for Menstrual Cramps in Women with Dysmenorrhea: A Prospective Clinical Observation,” P.-Y. Lin et al. explore questions related to the strengths, weaknesses, challenges, and accomplishments of TCM treatments based on Bian Zheng Lun Zhi theory, along with the results of active surveillance proactively in women with primary dysmenorrhea or endometriosis. The following three articles focus on the observational evaluation of integrative and/or complementary therapies in human populations. In a paper titled “Concurrent Use in Taiwan of Chinese Herbal Medicine Therapies among Hormone Users Aged 55 Years to 79 Years and Its Association with Breast Cancer Risk: A Population-Based Study,” Y.-T. Tsai et al. evaluate the breast cancer risk of consuming Chinese herbs, especially the extent to which the hormone users used Chinese herbs concurrently. In another paper titled “The Prescription Pattern of Chinese Herbal Products Containing Ginseng among Tamoxifen-Treated Female Breast Cancer Survivors in Taiwan: A Population-Based Study,” W.-L. Hsu et al. investigate the inhibitory relationship between Ginseng consumption and subsequent endometrial cancer among tamoxifen users. In a third article titled “Effects of Passive Hydrotherapy WATSU (WaterShiatsu) in the Third Trimester of Pregnancy: Results of a Controlled Pilot Study,” A. M. Schitter et al. carry out a controlled pilot study to assess the potential therapeutic effects of passive hydrotherapy on self-reported stress, pregnancy-related pain, mood and quality of life, amount of amniotic fluid, blood flow characteristics, spontaneous course of breech presentations, and prospects of external cephalic versions in women in their ≥34th week of gestation. In a paper titled “Preventive Effects of Collagen Peptide from Deer Sinew on Bone Loss in Ovariectomized Rats,” H. Zhang et al. test the preventive effect of deer sinew on ovariectomy-induced bone loss. In a paper titled “Prolonged Diuretic Activity and Calcium-Sparing Effect of Tropaeolum majus: Evidence in the Prevention of Osteoporosis,” L. N. Barboza et al. are considering the diuretic effects of prolonged administration of Tropaeolum majus in ovariectomized rats and the interrelationship between calcium excretion and bone turnover. In a paper titled “Propolis is an Efficient Fungicide and Inhibitor of Biofilm Production by Vaginal Candida albicans,” I. R. G. Capoci et al. test the minimum inhibitory concentration and the minimum fungicidal concentration and assess the cell viability, protein, and carbohydrate characteristics of the biofilm of clinical isolates from vulvovaginal candidiasis to Propolis.

鮭皮コラーゲンペプチドの牛蹄冠部周囲真皮への影響と効果

鮭皮コラーゲンペプチドの牛蹄冠部周囲真皮への影響と効果

Effects of Collagen-Peptide on Physical Properties of Agar Gels

Effects of Collagen-Peptide on Physical Properties of Agar Gels

Effects of collagen-peptide on physical properties of agar gels

Effects of collagen-peptide on physical properties of agar gels