Abstract
Active collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism.
Highlights
Active collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recentlyestablished ELISA
We investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA
The cross-reactivity of commercial CP, the hydrolysis product of collagen, was approximately 0.1-fold. These results show that the anti-ACOP antibody had strong cross-reactivity with Pro-Hyp and Gly-Pro-Hyp, binding to dipeptides and tripeptides and to the collagen-derived oligopeptide CP
Summary
Active collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recentlyestablished ELISA. We investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA. Collagen turnover, involving biosynthesis and degradation, is altered by the effects of aging[17], physical activity[18], and nutritional status[19] These factors may affect the status of collagen metabolites in the body. We determine the specific active collagen oligopeptides (ACOP) detected by this ELISA for use in monitoring collagen metabolism. We investigate the effect of CP ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA
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