Cultivation Of Edible Mushrooms Research Articles

Selection and identification of high yielding transgressive segregants in button mushroom

Present study is an attempt for the genetic improvement of commercially important white button mushroom through quantitative genetics. Systematic and continuous breeding programme is the critical for enhancing crop productivity. Among the cultivated edible mushrooms, white button mushroom occupies fourth prominent place in terms of production across the global.SSIs of high yielding non browning hybrid strain NBS-5 was selected for the study. A total number of 191 single spore isolates were isolated from the strain and evaluated under controlled conditions for the selection of segregants in the F 2 generation. Analysis of variance indicated significant differences among all the genotypes for yield and yield related traits. Genetic analysis showed significant influence of the growing environment on the expression of different traits. High heritability coupled with high genetic advance was observed for the average fruit body weight and stipe length suggesting that yield can be improved through direct phenotypic selection.

Pola Aktivitas Enzim Lakase, Selulase, dan Xilanase Pada Masa Pertumbuhan Budidaya Jamur Tiram Putih [Pleurotus ostreatus (Jacq.) P. Kumm.

Recently, Pleurotus ostreatus has become the most cultivated edible mushroom in Indonesia because of the simple in cultivation. Pleurotus ostreatus easy to grow in a medium contained sawdust as a main substrate, with the addition of rice brand, corn flour, lime and gypsum. Mushroom production is bioconversion process which involves the activity of lignocellulolytic enzymes such as laccase, cellulase, and xylanase. The aim of this study was to reveal the pattern of laccase, cellulase, and xylanase activity during the growth of P. ostreatus on the sawdust medium. The results show that the highest laccase activity was obtained on the 15th day after spawn inoculation (ASI) of 0.66 Unit and decline sharply after the day of 45th ASI until the day of 75th ASI. Furthermore, the similar pattern with difference on the time incubation was obtained on the activity of cellulase and xylanase. It was decrease after the day 30th ASI. The highest activity of cellulase was 0.51 Unit on 30th day of ASI and decrease gradually until the day of 75th ASI. Furthermore, the highest activity of xylanase was on 30th day ASI of 0.34 Unit and decrease sharply until the day of 75th.

Mushroom as a means of sustainable rural development in the Chin State, Myanmar

Restricted access to quality crops and markets limits the capacity of rural communities to generate income in the mountainous regions of South and Southeast Asia, often resulting in the exploitation of forest systems, a subsequent decline in forest health, and the need for alternative means of income generation as well as forest restoration programmes. Cultivation of edible and medicinal mushrooms has been shown to be a feasible alternative source of income and also contributes to the household nutrition of rural and impoverished communities. There are a number of ongoing programmes in South and Southeast Asia currently implementing trainings and demonstrative practices for the cultivation of mushrooms at the village level. In this context, we implemented different cultivation strategies in selected rural areas in the Chin State, Myanmar. The cultivation strategies were to 1) introduce mushroom cultivation using locally sourced raw materials as substrates for rural communities unfamiliar with mushroom cultivation; and 2) improve the technical knowledge of those already experienced in mushroom cultivation in order to increase yield and minimize challenges in production systems. We introduced <i>Pleurotus</i> sp. cultivation to mushroom growers in the Chin state, Myanmar. These cultivation strategies will help further improve and develop mushroom industries in rural areas and contribute to rural development. In addition, we focused on cultivation systems that incorporate the use of crop residues and woody substrates in order to ensure a sustainable, integrated approach.

Ganoderma research activities and development in Namibia: A review

Medicinal mushrooms have provided a natural source of bioactive compounds since ancient times. Ganoderma is such a mushroom, which has worldwide recognition as a medicinally important mushroom. In this review, the authors provide a detailed summary of indigenous Ganoderma research in Namibia, looking at the ethnomycology, biological activity, physicochemical properties, food quality and safety, as well as cultivation of this mushroom. In this paper, all available records of Ganoderma research in Namibia were retrieved from the University of Namibia Institutional Repository with the keyword “Ganoderma”. Ten theses and four peer-reviewed articles with a total of 10 authors and 12 subjects, including ethnomedicinal plants, domestication, active compounds, medicinal and AIDS were reviewed. The main objective of this review is to guide researchers on the direction for future research and product development of Ganoderma in Namibia. The literature review highlights the potential to establish research and development activities of indigenous Namibian Ganoderma species. The study identifies an important knowledge gap on Ganoderma research, such as the complete morphological and molecular description of species, regulation and standardization of metabolites and characterization of novel compounds identified in indigenous Namibian Ganoderma. The domestication and cultivation of medicinal and edible mushrooms provide an excellent opportunity to contribute to the United Nations Sustainable Development Goals of promoting good health and well-being (SDG 3), nutrition (SDG 2: Zero Hunger), and income generation (SDG 1: no poverty).

Composting of the waste of the heart of palm agroindustry for the cultivation of edible mushrooms

The agribusiness of heart of palm (Bactris gasipaes) generates large amounts of waste, causing environmental impacts. Before this waste is returned to crop soil, it can be used in the production of...

Genome sequence of the oyster mushroom Pleurotus ostreatus strain PC9.

The oyster mushroom Pleurotus ostreatus is a basidiomycete commonly found in the rotten wood and it is one of the most cultivated edible mushrooms globally. Pleurotus ostreatus is also a carnivorous fungus, which can paralyze and kill nematodes within minutes. However, the molecular mechanisms of the predator–prey interactions between P. ostreatus and nematodes remain unclear. PC9 and PC15 are two model strains of P. ostreatus and the genomes of both strains have been sequenced and deposited at the Joint Genome Institute (JGI). These two monokaryotic strains exhibit dramatic differences in growth, but because PC9 grows more robustly in laboratory conditions, it has become the strain of choice for many studies. Despite the fact that PC9 is the common strain for investigation, its genome is fragmentary and incomplete relative to that of PC15. To overcome this problem, we used PacBio long reads and Illumina sequencing to assemble and polish a more integrated genome for PC9. Our PC9 genome assembly, distributed across 17 scaffolds, is highly contiguous and includes five telomere-to-telomere scaffolds, dramatically improving the genome quality. We believe that our PC9 genome resource will be useful to the fungal research community investigating various aspects of P. ostreatus biology.

Different cultivation methods of Hericium erinaceus (Bull: Fr.) Pers. in Chhattisgarh

Among the cultivated edible and medicinal mushrooms, Lion’s Mane Mushroom (Hericium erinaceum (Bull. Fr.) Pers.) Is an important mushroom and grown in North America, Europe and Asia. It is also known as monkey head mushroom in China, and ‘cendawan bunga kobis’ in Malaysia. In nature, the fruit body grows out of the knotholes or wounds of a tree. The fresh, young fruit body is edible and is regarded as a delicacy. Different containers i.e. bottle, bucket and poly propylenebag methods were evaluated to see thier effect on spawn run and yield of H. erinaceus and poly propylene bag method was found superior then other methods to achieve maximum yield (191.33g) with 38.26% BE of H. erinaceus while least yield (107.0g) with 10.7% BE was observed on bucket methods.

Diseases, moulds, insect-pests and mites of mushroom

Mushroom is defined as macro-fungus with distinctive edible fruiting body which can be either epigeous or hypogeous. Cultivation of edible mushrooms carries great relevance in todays’ world in the context of a burgeoning population growth and extreme pressure on the environment. Mushrooms are highly nutritious and environment friendly crops that carry numerous medicinal benefits. The intensive cultivations of edible mushrooms can often be affected by several insect-pests and diseases caused by fungi, bacteria, viruses, nematodes etc. that rather frequently cause dramatic production loss. The market price of edible mushrooms is also reduced due mould’s contaminations. These infestations, infections and contaminations are facilitated by the particular environmental conditions under which mushroom cultivation is commonly carried out. There is not much bibliographic information related to such stresses of mushrooms and their management. The updated review presents a practical checklist of diseases and pests of the mushroom, providing useful information that may help different users.

Comparative transcriptome analysis reveals candidate genes related to cadmium accumulation and tolerance in two almond mushroom (Agaricus brasiliensis) strains with contrasting cadmium tolerance.

Cadmium (Cd) is a toxic metal occurring in the environment naturally. Almond mushroom (Agaricus brasiliensis) is a well-known cultivated edible and medicinal mushroom. In the past few decades, Cd accumulation in A.brasiliensis has received increasing attention. However, the molecular mechanisms of Cd-accumulation in A. brasiliensis are still unclear. In this paper, a comparative transcriptome of two A.brasiliensis strains with contrasting Cd accumulation and tolerance was performed to identify Cd-responsive genes possibly responsible for low Cd-accumulation and high Cd-tolerance. Using low Cd-accumulating and Cd-tolerant (J77) and high Cd-accumulating and Cd-sensitive (J1) A.brasiliensis strains, we investigated 0, 2 and 5 mg L-1 Cd-effects on mycelium growth, Cd-accumulation and transcriptome revealed by RNA-Seq. A total of 57,884 unigenes were obtained. Far less Cd-responsive genes were identified in J77 mycelia than those in J1 mycelia (e.g., ABC transporters, ZIP Zn transporter, Glutathione S-transferase and Cation efflux (CE) family). The higher Cd-accumulation in J1 mycelia might be due to Cd-induced upregulation of ZIP Zn transporter. Cd impaired cell wall, cell cycle, DNA replication and repair, thus decreasing J1 mycelium growth. Cd-stimulated production of sulfur-containing compounds, polysaccharides, organic acids, trehalose, ATP and NADPH, and sequestration of Cd might be adaptive responses of J1 mycelia to the increased Cd-accumulation. DNA replication and repair had better stability under 2 mg L-1 Cd, but greater positive modifications under 5 mg L-1 Cd. Better stability of DNA replication and repair, better cell wall and cell cycle stability might account for the higher Cd-tolerance of J77 mycelia. Our findings provide a comprehensive set of DEGs influenced by Cd stress; and shed light on molecular mechanism of A.brasiliensis Cd accumulation and Cd tolerance.

Telomere-to-telomere assembled and centromere annotated genomes of the two main subspecies of the button mushroom Agaricus bisporus reveal especially polymorphic chromosome ends

Agaricus bisporus, the most cultivated edible mushroom worldwide, is represented mainly by the subspecies var. bisporus and var. burnettii. var. bisporus has a secondarily homothallic life cycle with recombination restricted to chromosome ends, while var. burnettii is heterothallic with recombination seemingly equally distributed over the chromosomes. To better understand the relationship between genomic make-up and different lifestyles, we have de novo sequenced a burnettii homokaryon and synchronised gene annotations with updated versions of the published genomes of var. bisporus. The genomes were assembled into telomere-to-telomere chromosomes and a consistent set of gene predictions was generated. The genomes of both subspecies were largely co-linear, and especially the chromosome ends differed in gene model content between the two subspecies. A single large cluster of repeats was found on each chromosome at the same respective position in all strains, harbouring nearly 50% of all repeats and likely representing centromeres. Repeats were all heavily methylated. Finally, a mapping population of var. burnettii confirmed an even distribution of crossovers in meiosis, contrasting the recombination landscape of var. bisporus. The new findings using the exceptionally complete and well annotated genomes of this basidiomycete demonstrate the importance for unravelling genetic components underlying the different life cycles.

Bioactive small secondary metabolites from the mushrooms Lentinula edodes and Flammulina velutipes.

Mushrooms have been attracting attention as a source of bioactive compounds for the development of dietary supplements and medicines. Many researchers have reported pharmacological effects of edible mushrooms, and have isolated and identified bioactive substances. Lentinula edodes (shiitake) and Flammulina velutipes (enokitake) are the cultivated edible mushrooms that are popular throughout the world. In L. edodes, polyacetylenes and sulfur compounds have been shown to display antimicrobial activity. In F. velutipes, many types of bioactive terpenes have been reported from mycelium culture filtrate or solid culture substrate. This article reviews the bioactive metabolites of low-molecular weight from L. edodes and F. velutipes.

Türkiye'deki Bazı Doğa ve Kültürü Yapılan Yenebilir Mantarların Antioksidan Enzim Aktiviteleri

In this study; wild and cultivated edible mushrooms [Boletus edulis Bull.: Fr, Craterellus cornucopioides (L.) Pers., Lactarius deliciosus (L. ex Fr.) S.F.Gray, Laetiporus sulphureus (Bull.: Fr.) Murr., Marasmius oreades (Bolt. ex Fr.) Fr., Morchella conica Pers., Ramaria botrytis (Pers.: Fr.) Ricken, Tricholoma terreum (Schaeff.: Fr.) P. Kumm., Hericium erinaceus (Bull.: Fr.) Pers., Lentinula edodes (Berk.) Pegler, Ganoderma lucidum (Curt.: Fr.) P. Karst., and Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm. (1-4)] were obtained from different locations in Turkey. Phenylalanine ammonia lyase (PAL) enzyme activity, ascorbate peroxidase (APX), peroxidase (POD), and superoxide dismutase (SOD) activity changes and nitrate, β-carotene and lycopene levels were investigated in 15 samples to determine antioxidant enzyme capacity. As a result of the study, the highest amount of β-carotene and lycopene were determined in H. erinaceus. P. ostreatus-2 had the lowest amount of β-carotene, whereas Pleurotus ostreatus-1 had the lowest amount of lycopene. Species rich in nitrate content were C. cornucopioides and P. ostreatus-4. P. ostreatus-3 was the poorest species in terms of nitrate compared to other mushroom samples. PAL activity of mushrooms varied between 5.863 and 8.893 EU mg-1 protein. For APX values, P. ostreatus-4 had the highest value, while H. erinaceus species had the lowest value. Among mushroom species, the highest and the lowest POD values were determined in H. erinaceus and B. edulis, respectively. C. cornucopioides had the highest and P. ostreatus-3 had the lowest SOD values.

Lentinula edodes mushroom as an ingredient to enhance the nutritional and functional properties of cereal bars.

Lentinula edodes (shiitake) is the second most cultivated edible mushroom in the world; it has low lipid contents, high protein and it is source of vitamins and minerals. This study aimed to develop and to evaluate two sweet and two salty food bars containing shiitake. The binder elements were heated and then the dried elements were added. The bars were shaped, and the sensorial test was accomplished with hedonic scale of 9 points for analysis of texture, aroma, taste and appearance, and a 5-point scale for buying intention. The centesimal composition included percentages of moisture content, ashes, lipids, proteins and carbohydrate contents. Chemical elements of shiitake were quantified by Energy Dispersive X-ray Fluorescence. Glucans were determined using a commercial kit. Phenolic compounds were determined with the Folin-Ciocalteu reagent. The shelf life was evaluated by microbiological control, up to 180days, at temperatures of 25°C and 37°C. The sweet bar 1 (SwB1) had better sensory analysis and buying intention. Shiitake showed high concentrations of calcium, iron, phosphorus, potassium, zinc, manganese, phenolic compounds and glucans. SwB1-bar maintained shiitake nutritional characteristics. SwB1-bars did not present microorganisms for up to 180days of shelf life, neither at 25°C nor at 37°C, and they followed the standards determined by National Health Surveillance Agency. Sweet bars are an easy marketing alternative due to their stability, low-cost of production and good acceptance, as well as flexibility to add other functional ingredients beneficial to health, such as shiitake.

EVALUACIÓN DE TRES RESIDUOS AGROINDUSTRIALES COMO SUSTRATOS PARA CULTIVO DEL PLEUROTUS OSTREATUS VAR. FLORIDA

Se presentan resultados del proyecto de investigación "Desarrollo de tecnologías para la elaboración de alimentos a partir de setas comestibles a escala de laboratorio”, que se plantea el aprovechamiento de residuos agroindustriales de la localidad como sustrato. El objetivo es la evaluación de la cascarilla de arroz y el afrecho cervecero como sustratos puros para el cultivo del Pleurotus ostreatus, empleando la pulpa de café como referencia. Se utilizó la cepa CCEBI 3024 Pleurotus ostreatus var. Florida. Los parámetros estudiados fueron: eficiencia biológica, rendimiento, diámetro de los cuerpos fructíferos y precocidad. El estudio reveló que el afrecho cervecero no fue un medio propicio para la fructificación de la seta objeto de estudio en las condiciones de producción. El sustrato de referencia pulpa de café superó en cuanto a eficiencia, precocidad y rendimiento a la cascarilla de arroz; sin embargo, este último posee potencialidades para ser empleado en el cultivo de setas comestibles en la localidad de Camagüey por su gran disponibilidad, bajo precio y cercanía de las plantas generadoras, contribuyendo a minimizar su impacto en el medio ambiente. Palabras clave: Pleurotus ostreatus; sustrato, cascarilla de arroz, afrecho cervecero, pulpa de café.

Degradative Ability of Mushrooms Cultivated on Corn Silage Digestate.

The current management practice of digestate from biogas plants involves its use for land application as a fertilizer. Nevertheless, the inadequate handling of digestate may cause environmental risks due to losses of ammonia, methane and nitrous oxide. Therefore, the key goals of digestate management are to maximize its value by developing new digestate products, reducing its dependency on soil application and the consequent air pollution. The high nitrogen and lignin content in solid digestate make it a suitable substrate for edible and medicinal mushroom cultivation. To this aim, the mycelial growth rate and degradation capacity of the lignocellulosic component from corn silage digestate, undigested wheat straw and their mixture were investigated on Cyclocybe aegerita, Coprinus comatus, Morchella importuna, Pleurotus cornucopiae and Pleurotus ostreatus. The structural modification of the substrates was performed by using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. Preliminary in vitro results demonstrated the ability of P. ostreatus, P. cornucopiae and M. importuna to grow and decay hemicellulose and lignin of digestate. Cultivation trials were carried out on C. aegerita, P. cornucopiae and P. ostreatus. Pleurotus ostreatus showed the highest biological efficiency and fruiting body production in the presence of the digestate; moreover, P. ostreatus and P. cornucopiae were able to degrade the lignin. These results provide attractive perspectives both for more sustainable digestate management and for the improvement of mushroom cultivation efficiency.

Evaluation of Different Substrates for Yield and Yield Attributes of Oyster Mushroom (Pleurotus ostreatus) in Crop-livestock Farming System of Northern Ethiopia

Background: In Tigray region of Ethiopia there is high rate of malnutrition which is severely affecting productivity of households. Cultivation of edible mushrooms can help to diversify income and mitigate malnutrition in the region. Aim: A laboratory experiment was carried out at Aksum University, northern Ethiopia aimed at evaluating the yield and yield attributes response of oyster mushroom to five types of substrates namely cotton seed hull, teff straw, barley straw, sesame stalk and sawdust. Methods: The experiment was set up in a Completely Randomized Design (CRD). Results: Findings of the experiment revealed that the type of substrate significantly (P≤0.05) affected days to mycelium invasion, days to pinhead formation, days to fruiting bodies formation and yield of Pleurotus ostreatus. Cotton seed hull was fastest in days to mycelium invasion with 15.66 days although in statistically parity with teff straw, barley straw and sesame stalk. Besides, cotton seed hull gave shortest days to pinhead formation, and days to fruiting bodies formation with 20.33 and 22.33 days respectively. The highest yield of 2170.33 gm kg-1 of substrate was obtained at cotton seed hull followed by barley straw which gave 1486.90 gm kg-1 of substrate and Teff straw which gave 1264.74 gm kg-1 of substrate. Conclusion: The experiment highlighted that cotton seed hull and barley straw can be used for mushroom production in the study area.

Anti-inflammatory diterpenes and steroids from peels of the cultivated edible mushroom Wolfiporia cocos

Chemical investigation on the peels of the cultivated edible mushroom Wolfiporia cocos led to the identification of 13 diterpenes and 4 steroids, including two new abietane diterpenes (1 and 2) and one new pregnane steroid (14). Structures of new compounds were determined by analysis of NMR, MS, and electronic circular dichroism (ECD) data. All compounds were evaluated for cytotoxicity (K562 and HepG2), antimicrobial and anti-inflammatory activity. Compounds 15, 17 showed strong cytotoxicity on K562 cells with the IC50 values of 5.4 and 7.5 μM, respectively. Compounds 4, 9 and 17 displayed medium antibacterial activity against Staphylococcus aureus with MICs of 31.3, 48.5 and 12.5 μM, respectively. Compounds 1-10, 14 and 16 showed inhibitory activity on the NO (nitric oxide) release in LPS-induced RAW 264.7 cells with IC50 values at the range of 16.8–75.8 μM. This work confirms the potential of peels of W. cocos in the treatment of infection diseases.

Degradation of Aflatoxin B1 by a Sustainable Enzymatic Extract from Spent Mushroom Substrate of Pleurotus eryngii.

Ligninolytic enzymes from white-rot fungi, such as laccase (Lac) and Mn-peroxidase (MnP), are able to degrade aflatoxin B1 (AFB1), the most harmful among the known mycotoxins. The high cost of purification of these enzymes has limited their implementation into practical technologies. Every year, tons of spent mushroom substrate (SMS) are produced as a by-product of edible mushroom cultivation, such as Pleurotus spp., and disposed at a cost for farmers. SMS may still bea source of ligninolytic enzymes useful for AFB1 degradation. The in vitro AFB1-degradative activity of an SMS crude extract (SMSE) was investigated. Results show that: (1) in SMSE, high Lac activity (4 U g−1 dry matter) and low MnP activity (0.4 U g−1 dry matter) were present; (2) after 1 d of incubation at 25 °C, the SMSE was able to degrade more than 50% of AFB1, whereas after 3 and 7 d of incubation, the percentage of degradation reached the values of 75% and 90%, respectively; (3) with increasing pH values, the degradation percentage increased, reaching 90% after 3 d at pH 8. Based on these results, SMS proved to be a suitable source of AFB1 degrading enzymes and the use of SMSE to detoxify AFB1 contaminated commodities appears conceivable.

Identification of a SNP and development of a PCR-based allele-specific marker of the sporulation-deficient (sporeless) trait of the Tamogitake 108Y2D mutant using next-generation sequencing.

The mass scattering of basidiospores during the cultivation of edible mushrooms causes serious problems, such as allergic reactions in workers. Sporulation-deficient (sporeless) cultivars would be very useful for preventing these issues. We aimed to identify the single-nucleotide polymorphism (SNP) that is responsible for the single dominant sporeless mutation of the Tamogitake 108Y2D mutant using next-generation sequencing (NGS) and TILLING technology and to develop an allele-specific PCR marker for sporeless breeding. By comparing the sequences of the wild-type and its mutant genomes, we identified 685 mutation loci in gene regions and pinpointed one SNP only consistent with sporeless phenotype for 105 segregants, i.e., a C to T located at position 1,950 of the exonic region of a putative fungal transcription factor that generated a stop codon. We developed an allele-specific marker based on the identified SNP, and its high practicality was validated using tests against progenies from several hybrids and wild isolates from different geographical origins. Thus, the allele-specific PCR marker developed here will be useful for marker-assisted selection in the breeding of the sporeless trait of this mushroom. Furthermore, the technical success of SNP identification and marker development based on NGS genome data can help achieve efficient mutation breeding in mushrooms.

Вплив складу рослинних субстратів на ефективність культивування їстівних грибів Cyclocybe aegerita (V. Brig.), Pleurotus eryngii (DC.) Quel., Pleurotus citrinopileatus Singer та Flammulina velutipes (Curtis) Singer

Influence of plant substrate composition on the efficiency of edible mushrooms cultivation Cyclocybe aegerita (V. Brig.), Pleurotus eryngii (DC.) Quel., Pleurotus citrinopileatus Singer and Flammulina velutipes (Curtis) Singer. The possibility of using plant substrates based on straw and sunflower husk, with the addition of rape seeds and corn processing waste for the cultivation of edible xylotrophic mushrooms: poplar mushroom, lemon-hat oyster mushroom and winter mushroom (white and yellow races) was investigated. The optimal formula of the substrate was established, the use of which increases the biological effectiveness of the studied strains and reduces the time required for obtaining fresh fruit bodies. Keywords: lemon-cap oyster mushroom Pleurotus citrinopileatus, poplar mushroom Cyclocybe aegerita, winter mushroom Flammulina velutipes, biological efficiency, technological cyclemushrooms (Flammulina velutipes), biological efficiency, technological cycle.