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2348 Articles

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Articles published on Edible Fruits

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Quantitative ethnomedicinal studies of wild edible fruits used by the indigenous people of the Surghar range, Pakistan

Quantitative ethnomedicinal studies of wild edible fruits used by the indigenous people of the Surghar range, Pakistan

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  • Ethnobotany Research and Applications
  • Oct 31, 2023
  • Shahzad Hussain + 8
Open Access
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Respon Tanaman Timun (Cucumis sativus L.) Terhadap Pemberian Pupuk Kandang Ayam dan Berbagai Konsentrasi Pupuk Organik Cair Orrin

Cucumber (Cucumis sativus L.) of the pumpkin tribe or Cucurbitaceae is a plant that produces edible fruit. The fruit is usually harvested when it is not fully ripe to be used as a vegetable or refreshment, depending on the type. One way to add nutrients to the soil is by applying chicken manure. According to Haryanto (2016) in Rumondang et al., (2020) also explained that the use of organic fertilizers is an action to improve the environment for plant plants that can improve the quality of land in a sustainable manner and organic fertilizers are also very beneficial for increasing agricultural production both in quality and quantity. This research was conducted on the experimental garden plot of the Faculty of Agriculture, Indonesian Community Development University UPMI Medan, on Jl. Market Village Hall 12 Marindal II, Patumbak District, Deli Serdang Regency, North Sumatra Province. At an altitude of 1000 meters above sea level. With the time of research in May-July 2023. The research was carried out in a factorial manner with the basic pattern of a randomized block design (RBD) consisting of two factors. Factor I: Dosage of chicken manure (A), namely: A0 = Control, A1 = 1 Kg/Plot, A2 = 2 Kg/Plot. Factor II: Dosage of liquid organic fertilizer orrin (O), namely: O1 = Control, O2 = 5 ml/l/plant, O3 = 10 ml/l/plant. The results showed that the single effect of giving chicken manure at level A2 (2 kg/plot) had an effect on increasing the growth of plant length, (at the age of 4 weeks), number of leaves (at the ages of 2 weeks, 3 weeks and 4 weeks), number of fruits and weight. fruit. The interaction effect of giving chicken coop and Orrin liquid organic fertilizer had no effect on plant length, number of leaves, number of fruits and fruit weight. Keywords: Cucumber, Chicken Manure, Orrin Liquid Organic Fertilizer

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  • JURNAL AGROPLASMA
  • Oct 29, 2023
  • Yuriko Prasetio + 2
Open Access
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Quantitative determination of macro and micro elements and heavy metals accumulated in wild fruits analyzed by ICP-OES method.

The moisture amounts of wild fruits ranged from 51.07 (rosehip) to 88.49% (raspberry (yellow)). Wild strawberry generally contained the highest amounts of P, K and Ca. In addition, the highest Mg result was recorded in wild strawberry, fig and rosehip fruits. P and K amounts of wild edible fruits were recorded between 385mg/kg (blackberry) and 2538mg/kg (fig) to 6114 (medlar) and 18,613mg/kg (wild strawberry), respectively. Depending on the fruit variety and type, the microelements found in the highest amounts were Zn, Cu, Mn and B. Fe resultss of fruits were reported at very low levels. Fe results of wild edible fruits changed between 0.21 (apple) and 1.32mg/kg ((fig). Zn and Cu values of wild edible fruits were recorded between 1.39 (apple) and 16.4mg/kg (fig) to 1.54 (apple) and 18.4mg/kg (wild strawberry), respectively. Cu contents of raspberry (red), raspberry (yellow), blackberry, jujube and medlar fruits were found to be very close to each other. Arsenic (As) contents of wild fruits were recorded to be higher than those of other elements. In addition, some fruits have high heavy metal contents. The heavy metal found in the highest amounts in fruits was As, followed in descending order by Ali Ba, Se, Ni and Pb. The high As content of these fruits is likely due to the As content of the soil where the plants are grown and its contamination with As-rich industrial waste. While As contents of edible wild fruits are detected between 4.21 (rosehip) and 65.7 (wild strawberry), Al results of fruits were reported between 0.03 (rosehip) and 16.0μg/g (mulberry (white)).

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  • Environmental Monitoring and Assessment
  • Oct 25, 2023
  • Fahad Al-Juhaimi + 4
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First report of Cephalerous virescens causing Algal leaf spot of Averrhoa carambola in India.

Averrhoa carambola (Star fruit) is a drought resistant edible fruit belongs to family Oxalidaceae. It is native of Malaysia and further cultivation is extended to China, Southeast Asia, India and Northern South America. Star fruit has juicy texture and used in salads, beverages and traditionally it has been used for ayurvedic medicines in India, Brazil and China (Abduh et al. 2023). In early January 2023, we observed the symptoms of raised, more or less circular, orange to dark brown, velvet textured, scattered algal leaf spots (1-4 mm) on the upper surface of A. carambola leaves at College farm, Agricultural College, Aswaraopet (17.252039 latitude, 81.109573 longitude) (Supplementary Fig 1). The disease was observed in 2 hectare model orchard with incidence of 45% causing leaf defoliation and thereby reducing the yield and quality of fruits. Transverse section cutting of algal spots revealed the algal thalli at subcuticular region and causing necrosis of epidermal cells. Sporangiophores (n=20) raised from algal leaf spot were cylindrical, 4 to 5 celled, 200-450 µm long x 8-20 µm wide, and forming a head cell with suffultory cells and sporangia on the top. Sporangia (n=20) were spherical to elliptical, rusty brown and 17.5-29 µm long × 18-23.6 µm wide and the total number of sporangia produced by each sporangiophores varies from 1 to 6. Setae (n=20) were filamentous with three to six celled, 17.5-50 µm long × 2.5-7.5 µm wide (Supplementary Figure 2). In our collection, mature gametangia were not observed. Morphological characters were studied on 20 diseased leaf samples collected from randomly selected five plants. To isolate pathogen, fresh algal thalli (n=5) were scraped from host tissue, surface sterilized (70% alcohol (30 s), 1% sodium hypochlorite (30 s) and sterile distilled water (3 × 60 s), inoculated to trebouxia liquid media and incubated at 25 ± 2 °C with a 12 hours photoperiod for 72 hours (Vasconcelos et al. 2018). The resultant five algal filaments were subjected to PCR amplification. The primer pair PNS1/NS41 was used in a PCR to amplify a fragment of 18S rRNA (Davis and Kaur 2019). The 18S rRNA gene sequences of the algae were compared using the Basic Local Alignment Search Tool (BLAST; http://www.ncbi.nlm.nih.gov/Blast/Blast.cgi) showed that our partial sequence had 99.5% similarity to C. virescens (KM020142.1). Hence, it was classified as C. virescens and sequences was deposited in NCBI-GenBank with accession numbers (OR053653, OR243777, OR429406, OR429407 and OR243779). For proving pathogenicity, algal filaments obtained from trebouxia liquid media were inoculated to 6 months old healthy A. carambola plant. Pathogenicity test was negative and typical symptoms could not be produced even up to 150 days of inoculation. In previous studies also, due to difficulty with production of zoospores in synthetic media, Koch's postulates of C. virescens as a plant pathogen has not been demonstrated experimentally (Sunpapao et al. 2017; Sanahuja et al. 2018; Kumar et al. 2019). In the second experiment, zoosporangia spore suspension were prepared from small pieces of algal leaf spot tissue processed in a sterile pestle and mortar and filtered through sterile cheesecloth (Sunpapao et al. 2017). A total of five isolates of zoosporangia spore suspension (1 x 102 to 1 x 104/ml of water) was sprayed on healthy, surface sterilized leaves of A. carambola plants (n=5) until runoff with a handheld airpump sprayer and incubated in green house (T: 25 oC, H: 80%). During the experiment leaves were remain attached to plant (5 days old) and plants were 6 months old grown in plastic pots under controlled conditions. Two plants were inoculated with each isolate and three non inoculated control plants were included. Non inoculated controls were sprayed with sterile distilled water. The pathogenicity experiment was repeated. The initial symptoms were produced 60 days after inoculation and complete algal thalli was observed on 90 days after inoculation, control plants were without any symptoms upto 150 days. Reisolated algal thalli from symptomatic plants were morphologically similar to original algal thalli and molecularly identified as C. virescens (accession number OR067193 and OR243810). Red rust caused by C. virescens is a major algal disease in the world and causing severe leaf defoliation in various horticultural crops viz., Mangifera indica (Vasconcelos et al. 2018), Manilkara zapota (Sunpapao et al. 2017), Psidium guajava (Rajbongshi et al. 2022), Ziziphus mauritiana (Shareefa et al. 2022) and Anacardium occidentale (Dooh et al. 2022). The available literature suggest that, this is the first report of algal leaf spot on A. carambola caused by C. virescens in India. This report extends the range of known pathogens associated with A. carambola plant and serves as a basis for development and implementing disease management strategies.

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  • Plant disease
  • Oct 24, 2023
  • B Deepak Reddy + 9
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Non-Timber Forest Products of Koppal District, Karnataka: Indigenous Use

The purpose of this study is to evaluate non-timber forest products in the Koppal districts of Karnataka. The report found the involvement of the local inhabitants in the collection of various NTFPs and the result is structured on a survey conducted from 2020 to 2023, resulting in the documentation of a total 114 species under 48 families and 94 genera identified as NTFPs, Among these trees were dominated with 50(44.24%), followed by shrubs 19(16.18%), herbs 30(27%), and climbers 14(12.38%), which were used in different categories by the locals, such as medicinal, wild edible fruit and vegetables, fodder, and domestic purposes. In different category of utility leaf were used most with 48(48%), followed by fruits 37(33%), seed 11(10%), whole plant 9(8%), stem 8(7%), shoot & flower 7(4%), root 6(5.3%) & dye & latex 1(0.88%).

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  • International Journal For Multidisciplinary Research
  • Oct 24, 2023
  • M Siddeshwari + 1
Open Access
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Edible Fruits from the Ecuadorian Amazon: Ethnobotany, Physicochemical Characteristics, and Bioactive Components.

In the Ecuadorian Amazon region, there are various types of edible fruits that have distinct qualities and benefits. Understanding the uses, properties, and functions of these fruits is important for researching products that are only available in local markets. This review aims to gather and summarize the existing scientific literature on the ethnobotany, physicochemical composition, and bioactive compounds of these native fruits to highlight the potential of the region's underutilized biodiversity. A systematic review was carried out following the PRISMA methodology, utilizing databases such as Web of Science, Scopus, Pubmed, Redalyc, and SciELO up to August 2023. The research identified 55 edible fruits from the Ecuadorian Amazon and reported their ethnobotanical information. The most common uses were fresh fruit consumption, preparation of typical food, and medicine. Additionally, nine native edible fruits were described for their physicochemical characteristics and bioactive components: Aphandra natalia (Balslev and Henderson) Barfod; Eugenia stipitate McVaugh; Gustavia macarenensis Philipson; Mauritia flexuosa L.f; Myrciaria dubia (Kunth) McVaugh; Oenocarpus bataua Mart; Plukenetia volubilis L.; Pouteria caimito (Ruiz and Pav.) Radlk.; and Solanum quitoense Lam. The analyzed Amazonian fruits contained bioactive compounds such as total polyphenols, flavonoids, carotenoids, and anthocyanins. This information highlights their potential as functional foods and the need for further research on underutilized crops.

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  • Plants
  • Oct 21, 2023
  • Maritza Sánchez-Capa + 2
Open Access
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Landscaping with Fruits: Citizens’ Perceptions toward Urban Horticulture and Design of Urban Gardens

Urban horticulture represents a sustainable way of dealing with the challenges of modern cities, including urbanization processes, population growth tendencies, a lack of green areas, environmental pollution and food insecurity. This study aimed to (1) investigate the perceptions of citizens of Novi Sad municipality, Republic of Serbia, toward urban horticulture and (2) assess plant materials appropriate for cultivation in urban gardens and suggest urban spaces completely designed with the use of various forms of edible fruit species, including roses. The same questionnaire was administered with a four-year time gap, in 2019 and again in 2023, before and after the coronavirus pandemic. To investigate plant materials suitable for urban gardening, relevant articles in Google Scholar, Scopus and Web of Science platforms were searched. The questionnaires’ results showed significant differences between the two years concerning respondents’ attitudes toward urban horticulture’s impact on food safety, socialization and personal health and development, which could have been influenced by the pandemic. Vegetables were grown by most respondents, commonly in household yards and gardens. The main motivations for participating in community urban gardens were to grow food for their own consumption and to make charitable contributions, followed by the motivation ‘to socialize and relax’. Exemplary designs of an urban pocket, kindergarten yard and atrium courtyard are presented, providing insights into numerous possibilities for landscaping with fruits.

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  • Horticulturae
  • Oct 20, 2023
  • Tijana Narandžić + 6
Open Access
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Jackal Jujube Into a Rts Beverage

Jackle jujube is one of the tribal fruit seen all over india. A variety of wild plants previously used by rural and tribal communities.There are many findings indicating the importance of wild fruit species as a crucial source of nutrition for rural impoverished people.Certain wild edible plants and fruits are significant components of biodiversity, and their exploitation has developed into a crucial livelihood strategy and backup plan for rural communities during times of natural hardship.These findings validate the traditional application of jackal jujube (Ziziphus oenoplia )to treat a range of ailments.These research talk about making a RTS(ready-to-serve) from jackal jujube fruit as one of the value added product.

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  • International Journal For Multidisciplinary Research
  • Oct 18, 2023
  • Nithya Sneha S.K + 2
Open Access
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Edible fruits and berries as a source of functional polyphenols: current scene and future perspectives

Edible fruits and berries as a source of functional polyphenols: current scene and future perspectives

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  • Phytochemistry Reviews
  • Oct 17, 2023
  • Chunpeng Wan + 12
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Protective effects of myricetin on airway inflammation and oxidative stress in ovalbumin-induced asthma mice

Myricetin, a flavonoid isolated from many edible vegetables and fruits, has multiple biological effects, including anti-inflammatory and anti-tumor effects. Myricetin could inhibit mast cell degranulation in vitro, and it reduced the eosinophil content in bronchoalveolar lavage fluid (BALF) of ovalbumin (OVA)-sensitized mice. However, it remains unclear whether myricetin alleviates airway hyperresponsiveness (AHR), airway inflammation, and oxidative stress in asthma. Here, we investigated whether myricetin attenuated AHR, airway inflammation, and eosinophil infiltration in lungs of asthmatic mice. Mice were sensitized with OVA, then injected intraperitoneally with myricetin to investigate anti-inflammatory and antioxidant effects of myricetin. Moreover, we examined its effects on human bronchial epithelial BEAS-2B cells stimulated with TNF-α and IL-4, in vitro. Myricetin effectively mitigated eosinophil infiltration, AHR, and goblet cell hyperplasia in lung, and it reduced Th2 cytokine expression in BALF from asthmatic mice. Myricetin effectively promoted glutathione and superoxide dismutase productions and mitigated malondialdehyde expressions in mice by promoting Nrf2/HO-1 expression. Myricetin also reduced the production of proinflammatory cytokines, eotaxins, and reactive oxygen species in BEAS-2B cells. Myricetin effectively suppressed ICAM-1 expression in inflammatory BEAS-2B cells, which suppressed monocyte cell adherence. These results suggested that myricetin could effectively improve asthma symptoms, mainly through blocking Th2-cell activation, which reduced oxidative stress, AHR, and airway inflammation.

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  • The Journal of Nutritional Biochemistry
  • Oct 14, 2023
  • Wen-Chung Huang + 4
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Pre-Hispanic use of edible Geoffroea decorticans fruits in central Argentina - first approximations based on an integrated morphoanatomical and archaeobotanical approach

Pre-Hispanic use of edible Geoffroea decorticans fruits in central Argentina - first approximations based on an integrated morphoanatomical and archaeobotanical approach

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  • Vegetation History and Archaeobotany
  • Oct 14, 2023
  • Valentina Saur Palmieri + 3
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Transfer of the traditional knowledge on edible plants and fruits in Anatolia in terms of gastronomic value: the case of Sivas and Yozgat

The study aims to determine the types of edible wild plants and fruits consumed in the culinary cultures of Yozgat and Sivas, their ethnobotanical characteristics, and the areas and forms of their uses. In the research, document analysis and semi-structured interviews were used as data collection techniques, and descriptive analysis was used as the data analysis method. The categories obtained as a result of the interview findings and the literature review consist of the areas of the uses of edible wild plants and fruits in the kitchen, their use patterns, their relationship with health, and the methods of cultivating and preserving edible wild plants and fruits. Research findings indicate that the tradition of using edible wild plants and fruits is still alive in Yozgat and Sivas.

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  • Journal of Tourism Theory and Research
  • Sep 30, 2023
  • Muhabbet Çeli̇k
Open Access
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Exploring the nutritional potential and anti-nutritional components of wild edible fruits of the Eastern Himalayas

Exploring the nutritional potential and anti-nutritional components of wild edible fruits of the Eastern Himalayas

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  • Journal of Food Measurement and Characterization
  • Sep 29, 2023
  • Thejangulie Angami + 10
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Multigenerational differences in harvesting and use of wild edible fruits and nuts in the South Caucasus

Societal Impact StatementEcosystem services are underpinned by biodiversity, which is rapidly eroding globally, threatening rural livelihoods and culture. Examining the uses of wild edible plants (WEPs) that are important to rural communities gives insight into the value of a biodiverse landscape to local communities. Here, the importance of considering age groups in future ethnobotanical and conservation studies is highlighted, as this can enhance our understanding on the dependence of use within a landscape, informing more inclusive conservation actions.Summary Wild edible plants (WEP) remain an important aspect of many rural communities across the world, yet the decline in the diversity and knowledge of WEP use is becoming a global concern. In the Biodiversity Hotspot of the Caucasus, there is few cross‐cultural and multigenerational comparisons of plant uses, limiting our knowledge of resource use and dependence within biodiverse landscapes. Here, we investigate the patterns of use for wild edible fruits and nuts in the South Caucasus, focusing on multigenerational differences in harvesting patterns, diversity and use through semi‐structured interviews in Armenia and Georgia. We calculated use values (UV) for each genera harvested and compared the diversity of genera used between age groups. Pearson chi‐square was used to explore the relationship between age‐groups and genera harvested. We found 53% of rural population (n = 220) actively harvest from wild populations, with older age groups harvesting the highest diversity of plants. Twenty‐four species from 16 genera are harvested, with Berberis vulgaris L. and Rosa canina L. shared between both communities. The association between age and diversity of harvested genera was significant (χ2[48, N = 506] = 114.75, p < .01), mainly driven by a strong positive association with Berberis spp. L., Crataegus spp. L. and Ribes spp. L. with the under 18s and Prunus spp. L. with under 35s. Young harvesters used WEP for income generation, while medicinal use increases across older age groups. Foraging activities within South Caucasus' communities remains active; however, the use of WEP is not uniform within and across different communities.

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  • PLANTS, PEOPLE, PLANET
  • Sep 27, 2023
  • Aisyah Faruk + 13
Open Access
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Momordica charantia (bitter gourd), a potent antidiabetic phytomedicine

Gourd bitter Grown mostly in India, China, and Southeast Asia, the tropical creeping plant known as or bitter melon. Its edible fruit is the primary reason for the plant's cultivation. Because of their bitter flavour, bitter gourds are not well-liked. The fruits of the Cucurbitaceae family plant Momordica charantia are a good source of a number of important nutrients. Over sixty phytomedicines, including blood purifiers, antioxidants, anticancer agents, and diabetes inhibitors, are present in the entire plant. In-depth information about the nutritional elements, different bioactive substances, and significant nutraceutical qualities of the bitter gourd plant in relation to diabetes are attempted to be provided in this review.

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  • International Journal of Pharmacognosy and Chemistry
  • Sep 23, 2023
  • Anil Kumar Anil + 1
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Fisetin, a potential skin rejuvenation drug that eliminates senescent cells in the dermis.

Accumulation of senescent fibroblasts, chronic inflammation, and collagen remodeling due to aging-related secretory phenotypes have been hypothesized to cause age-related skin aging, which results in wrinkles and loss of skin elasticity, thus compromising appearance attractiveness. However, the rejuvenating effects of removing senescent cells from the human skin and the efficacy of related therapeutic agents remain unclear. Here, we investigated the effects of fisetin, a potential anti-aging component found in various edible fruits and vegetables, on senescent human dermal fibroblasts (HDFs) and aging human skin. Senescence was induced in primary HDFs using long-term passaging and treatment with ionizing radiation, and cell viability was assessed after treatment with fisetin and a control component. A mouse/human chimeric model was established by subcutaneously transplanting whole skin grafts from aged individuals into nude mice, which were treated intraperitoneally with fisetin or control a component for 30 d. Skin samples were obtained and subjected to senescence-associated-beta-galactosidase staining; the extent of aging was evaluated using western blotting, reverse transcription-quantitative PCR, and histological analysis. Fisetin selectively eliminated senescent dermal fibroblasts in both senescence-induced cellular models; this effect is attributable to cell death induction by caspases 3, 8, and 9-mediated endogenous and exogenous apoptosis. Fisetin-treated senescent human skin grafts showed increased collagen density and decreased senescence-associated secretory phenotypes (SASP), including matrix metalloproteinases and interleukins. No apparent adverse events were observed. Thus, fisetin could improve skin aging through selective removal of senescent dermal fibroblasts and SASP inhibition, indicating its potential as an effective novel therapeutic agent for combating skin aging.

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  • Biogerontology
  • Sep 22, 2023
  • Kento Takaya + 2
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Sensory Evaluation, Biochemical, Bioactive and Antioxidant Properties in Fruits of Wild Blackthorn (Prunus spinosa L.) Genotypes from Northeastern Türkiye

Wild edible fruits are an important source for agriculture worldwide suffering from genetic erosion due to a severe genetic diversity reduction and domestication hindrance. In Türkiye, underutilized Prunus spinosa fruits are increasingly being considered as genetic resources and are marginally used by small farmers constituting a real safety valve for the sustainability of the processing plum value chain. Fruits of those plum genotypes differ in their biometric, processing and functional quality attributes. In this study, fruits of eight wild grown blackthorn (Prunus spinosa) genotypes were sampled from the Ispir district of the Erzurum province and subjected to sensory, morphological, biochemical and antioxidant characterization. Aroma, taste and juiciness were used as the criteria for sensory analysis, and a trained panel of ten experts established and evaluated the sensory characteristics of the fruits of the blackthorn. Fruit weight, fruit skin and flesh color as L*, a* and b* values were the main morphological parameters. For biochemical and bioactive analysis, organic acids, SSC (Soluble Solid Content), vitamin C, total anthocyanins, total phenolic content and total antioxidant capacity were determined. Antioxidant capacity was determined by FRAP (ferric reducing antioxidant power) assay. The results indicated significant differences among genotypes for most of the traits. The fruit weight was found between 2.78–3.67 g. The skin L*, a* and b* values were 13.11–16.12, 2.56–3.85 and 2.01–3.44, respectively. The flesh L*, a* and b* values were in the ranges of 17.45–20.37, 4.88–6.73 and 4.12–5.66, respectively. The SSC content ranged from 18.66% to 21.07%. The total phenolic content (TPC), total anthocyanin content (TAC) and ferric reducing antioxidant power (FRAP) were between 372–504 mg GAE/100 g; 53–72 mg cy-3 g eq./100 g and 107–134 mmol Fe (II) eq./g, respectively. The dominant organic acid was malic acid for all genotypes and varied from 1.04 g/100 g to 1.52 g/100 g fresh weight base. The data showed that the analyzed blackthorns, particularly PS-5, PS-3 and PS-2 had bigger fruits indicate their suitability for fresh and dried consumption, PS-1 and PS-3 had higher juiciness, indicating their suitability for processing, and PS-4 and PS-6 had higher human health promoting compounds (higher total phenolic content and antioxidant capacity), making them suitable for future use as functional foods and as promising sources of natural antioxidants.

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  • Horticulturae
  • Sep 19, 2023
  • Gulce Ilhan
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Evidence that the domesticated fungus Leucoagaricus gongylophorus recycles its cytoplasmic contents as nutritional rewards to feed its leafcutter ant farmers

Leafcutter ants farm a fungal cultivar (Leucoagaricus gongylophorus) that converts inedible vegetation into food that sustains colonies with up to millions of workers. Analogous to edible fruits of crops domesticated by humans, L. gongylophorus has evolved specialized nutritional rewards—swollen hyphal cells called gongylidia that package metabolites and are consumed by ant farmers. Yet, little is known about how gongylidia form, and thus how fungal physiology and ant provisioning collectively govern farming performance. We explored the process of gongylidium formation using advanced microscopy to image the cultivar at scales of nanometers, and both in vitro experiments and in silico analyses to examine the mechanisms of gongylidia formation when isolated from ant farmers. We first used transmission electron, fluorescence, and confocal microscopy imaging to see inside hyphal cells. This imaging showed that the cultivar uses a process called autophagy to recycle its own cellular material (e.g. cytosol, mitochondria) and then shuttles the resulting metabolites into a vacuole whose continual expansion displaces other organelles and causes the gongylidium cell’s bulging bulb-like appearance. We next used scanning electron microscopy and light microscopy to link this intracellular rearrangement to the external branching patterns of gongylidium cells as they clump together into edible bundles called staphyla. We next confirmed that autophagy plays a critical role in gongylidium formation both: (1) in vitro as gongylidium suppression occurred when isolated fungal cultures were grown on media with autophagy inhibitors, and (2) in silico as differential transcript expression (RNA-seq) analyses showed upregulation of multiple autophagy gene isoforms in gongylidia relative to undifferentiated hyphae. While autophagy is a ubiquitous and often highly derived process across the tree of life, our study reveals a new role for autophagy as a mechanism of functional integration between ant farmers and their fungal crop, and potentially as a signifier of higher-level homeostasis between uniquely life-time committed ectosymbionts.

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  • IMA Fungus
  • Sep 15, 2023
  • Caio Ambrosio Leal-Dutra + 5
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ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acid-induced anthocyanin biosynthesis in Cerasus humilis

Cerasus humilis is a kind of economic fruit tree peculiar to China, which is widely used in the food, landscape, and pharmaceutical industries. Anthocyanins are a phenolic metabolite that plays an essential role in fruit coloration. However, the regulatory network of C. humilis in anthocyanin biosynthesis is still unclear. In this study, the R2R3-MYB transcription factor ChMYB1 was isolated from the full genome of the species. Yeast one-hybrid, dual-luciferase assays, and GUS staining showed that ChMYB1 significantly increased anthocyanin contents in C. humilis fruit by promoting the expression of ChCHS and ChUFGT by binding MBS (MYB-binding elements). ChMYB1 interacted with ChbHLH42 and ChTTG1 to form the MBW complex and further enhanced the expression of ChUFGT. In addition, abscisic acid (ABA) treatment promoted the expression of ChMYB1 and anthocyanin accumulation in C. humilis fruit. Interestingly, ABA treatment enhanced the interaction between ChMYB1 and ChbHLH42. Furthermore, ChABI5 inhibited the interaction between ChMYB1 and ChbHLH42. Our data elucidated the primary molecular mechanism of anthocyanin biosynthesis in C. humilis fruit, deepening the understanding of the regulatory network affecting anthocyanin metabolism in edible fruit crops.

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  • Horticultural Plant Journal
  • Sep 15, 2023
  • Xiaolong Ji + 4
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Biochemical and physiological mechanisms allowing olive trees to survive and produce under water stress conditions

The beneficial properties of olive (Olea europaea L.) edible fruits and their oil play an important part in our diet. Severe summer conditions, including low rainfall, excessive heat load and high daily irradiance, characterize arid and semiarid regions where olive is produced. Although it is highly aggravated by other stresses, water stress is usually the most critical during summer-time. Under severe water stress conditions, a cultivar of any olive tree needs specific biochemical and physiological mechanisms in order to survive and be productive. It is well-known that these water stress resistance mechanisms generally act simultaneously. Nevertheless, water stress adaptability not only integrates the water stress resistance concept (i.e., escape, avoidance and/or tolerance to water stress) but also the recovery capacity. All of them play a major role in plants’ growth and survival, especially when plants are continuously exposed to repeated events of water stress and adequate water levels during their lifetime. Water stress, heat and high irradiance, especially in association with each other, also damage plant functions. As a result, different resistance mechanisms are adopted by plants. This review summarizes each of these mechanisms.

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  • Lilloa
  • Sep 14, 2023
  • Mariano A Busso
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