ABSTRACT To simulate and debug or diagnose manufacturing systems is always challenging. This paper presents a hybrid PLC-controlled and PC Visual Basic (VB)-based Human-Machine Interface (HMI) platform based on Petri net (PN) theory for Manufacturing Systems. An augmented PN model was adopted to design and simulate the operational sequence of the Flexible Manufacturing System (FMS) at the National Kaohsiung First University of Science and Technology (NKFUST). With the simulated PN model, the control sequences were quickly translated into PLC programs. A PC-based HMI monitoring system based on the PN model was developed to provide an intuitive offline simulation and online diagnostic tools for the FMS. This hybrid system shows many of advantages by using the economical discrete control unit with PLC, and PC-based HMI using PN model as a base. The discrete event control sequence of an FMS can be easily modeled and evaluated before its build-up. More importantly, it can be diagnosed online when the system is malfunctioning. That is, the presented system provides an adequate means for offline system design, simulation, performance evaluation, and quick online system diagnostic.