Dynamics Of Biochemical Parameters Research Articles

Biochemical parameters of chicken blood under the influence of technological stimuli of various etiologies

Violation of the main technological parameters of keeping and feeding poultry is the main factor in reducing egg productivity and health in the industrial production system. Therefore, the research aims to determine biochemical markers in acute technological stress caused by factors of various etiologies. In this study, serum biochemical parameters were evaluated for the effects of stimuli in egg-laying chickens. The study was conducted on several Hy-Line W-36 cross chickens in the conditions of a production line. Four groups of chickens (101 heads each) aged 52 weeks were formed to conduct the study. The control group was formed from 10 representatives of each of the groups. Further, each group was exposed to a corresponding technological stimulus. Birds of group 2 were not fed for 24 hours, those of group 3 were kept in a darkened place, and 60 additional birds were placed in a cage with the chickens of group 4. The highest effect on the dynamics of biochemical parameters was an increase in their concentration by 1 m2 cage space. The studies identified a substantial increase in the concentration of glucose and creatinine in the blood serum of chickens during over-compaction – by 24-28% (p<0.05) compared to the control group, regardless of the cause of the stressful situation, all birds exposed to acute stress had an increase in these indicators in the blood serum. The main part of the examined enzymes, such as aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase, tended to increase their activity depending on the strength of the influence of the technological factor on the body of birds. Dynamic characteristics of glucose and creatinine levels were distinguished among the examined biochemical parameters by rapid and differentiated reactions within the physiological norm, and considering the simplicity of their determination, they may have prospects of becoming the main markers of early diagnosis of stress in industrial poultry farming

Изменчивость биохимических признаков Pinus sylvestris (Pinaceae) при адаптации форм в условиях избыточного увлажнения

Conifers are characterized by a certain degree of individual variability in the content of stress metabolites, which can be caused by genotypic variations or divergence in the growth conditions of individual trees. The purpose of the study is to evaluate the variation of biochemical traits of Scots pine, which are different in the color of male strobilus, during adaptation to the conditions of constant excessive moisture in the soil of the northern taiga. The dynamics of metabolic parameters of Pinus sylvestris L. were investigated in shrubsphagnum pine stands on marshy upland soils at the mouth of the Northern Dvina River (northern taiga subzone). From July to November 2018, needle samples from shoots were collected from 10 pine trees of each of the male strobilus types, differentiated by color. The contents of photosynthetic pigments, anthocyanins, ascorbic acid, proline, water-soluble proteins, and pH were measured under laboratory conditions. The synthesis of chlorophyll in the needles in the current year of formation decreased in trees of both forms during the dry summer period. A favorable temperature in the autumn period could promote an extension of photosynthetic pigment accumulation, which might negatively affect tree preparation for overwintering. There were no significant differences between f. erythranthera Sanio and f. sulfuranthera Kozubow in the needle content of chlorophyll and carotenoids, anthocyanins, ascorbic acid, free proline, water-soluble proteins, or pH. The similarities in the adaptation of the trees to the constant excessive soil moisture in northern taiga conditions were revealed. The seasonal factor had a considerable impact on the dynamics of biochemical parameters. Ascorbic acid, proline, and anthocyanin were more actively accumulated in the needles throughout the summer due to the high air temperature, resulting in an increase in antioxidant activity and the development of protective mechanisms aimed at reducing oxidative stress. The increase in water-soluble protein content in needles from October to November is one of the cryoprotective mechanisms of the trees for the winter period. Individual differences in ascorbic acid and proline contents in yellow microspore-bearing pine needles at the establishment of minus temperatures in November were considerably higher than in red microspore-bearing pine needles. This is regarded as the norm of the reaction for different types of trees to the influence of negative temperatures. For citation: Tarkhanov S.N., Pinaevskaya E.A., Aganina Y.E., Pakhov A.S. Variability of Biochemical Properties During Adaptation of Pinus sylvestris (Pinaceae) Forms to Excessive Moisture Conditions. Lesnoy Zhurnal = Russian Forestry Journal, 2023, no. 4, pp. 58–75. (In Russ.). https://doi.org/10.37482/0536-1036-2023-4-58-75

ГЕМАТОБИОХИМИЧЕСКИЙ СТАТУС СОБАК ПРИ ИСПОЛЬЗОВАНИИ БИОКОМПОЗИЦИОННОГО ПОКРЫТИЯ ИМПЛАНТОВ ДЛЯ УСКОРЕНИЯ КОНСОЛИДАЦИИ КОСТНОЙ ТКАНИ

Injuries of the musculoskeletal system of dogs occur in more than 30% of the total number of non-infectious pathologies. In veterinary and humane traumatology, the problems associated with fractures and their complications have not been fully resolved. Due to the use of different methods of osteosynthesis using different implants (spokes, bone plates, osteofixers, pins), it is quite important to apply coatings for these materials that met such specified needs as: thickness, morphological heterogeneity, roughness, porosity, adhesive strength and antibacterial effect to accelerate the consolidation processes. The article shows the results of studies on the dynamics of hematological and biochemical parameters of groups of experimental animals (dogs), in view of the use of osteoplastic biocomposition coating for implants (spokes) in intramedullary and extra-focal osteosynthesis of tubular bones. Animal blood samples (n=120) served as the material for research. The purpose of this study was to assess the dynamics of hematological and biochemical parameters of blood during the implantation of spokes with the developed osteoplastic biocomposition coating. A group of authors, based on the dynamics of hematological parameters, determined that the coating developed by them, consisting of hydroxyapatite, methyluracil, amoxicillin and polylactide, does not cause hematopoiesis inhibition and acute inflammatory reaction. Based on the dynamics of biochemical parameters, it was determined that this coating does not have hepatotoxicity and nephrotoxicity, the absence of a negative effect of this material on the process of bone restoration and the formation of a callus was determined. The developed coating, from the point of view of the authors, allows for a sufficiently high degree and speed of consolidation of bone fragments with the prevention of bacterial contamination.

The use of a combined herbal remedy for some functional bowel diseases is the implementation of the concept of multitarget therapy

Aim of investigation: to evaluate the effectiveness and safety of the use of dietary supplement "Tummy ease" in the treatment of patients with functional constipation and IBS with a predominance of constipation. Materials and methods: The treatment of 33 patients was analyzed. 18 patients with FС (2 men and 16 women, mean age 50.6 ± 4.0 g) and 15 patients with IBS-C (2 men, 13 women, mean age 51.8 ± 4.5 years). Patients received Tummy ease 2-3 capsules/day for 28 days. The safety of treatment was assessed by the dynamics of biochemical parameters, the frequency and presence of side effects. To evaluate the effectiveness, we studied: the time of the carbolenic test, the dynamics of constipation symptoms according to the sum of points of the counting scale for assessing the severity of constipation and the PAC-SYM questionnaire, the dynamics of symptoms of the gastrointestinal tract according to the GIS questionnaire, the dynamics of quality of life according to the GSRS questionnaire. Results of the study: in patients with FC and IBS, there was a significant decrease in the symptoms of constipation (difficulty/pain during defecation, feeling of incomplete emptying, abdominal pain, time spent in the toilet, assistance with defecation, unsuccessful attempts to empty) and an increase in the frequency of defecation. According to the PAC-SYM questionnaire, patients with FC and IBS-C showed a significant decrease in abdominal discomfort, bloating, straining, feeling of incomplete emptying, defecation scarcity and difficulty, and the total symptom score. Patients with IBS-C additionally noted a decrease in abdominal pain, painful bowel movements and the number of false urges. The time of the carbolenic test significantly decreased in patients with FC from 49.4 ± 3.8 to 30.0 ± 2.3 hours (p = 0.00003), in patients with IBS-C from 62.7 ± 6.9 to 43.8 ± 6.4 hours (p= 0.002). In patients with FC and IBS-C, there was a significant positive dynamics of the symptoms of the gastrointestinal tract according to the GIS questionnaire and a significant improvement in the quality of life according to the GSRS questionnaire. Conclusion: The drug (BAA) Tummy ease is effective and safe in the treatment of patients with functional constipation and IBS-C and can be used as an alternative therapy.

ДИНАМИКА КЛИНИКО-БИОХИМИЧЕСКИХ ПОКАЗАТЕЛЕЙ КРОВИ ЖИВОТНЫХ ПРИ ПРИМЕНЕНИИ СПИЦ С ИННОВАЦИОННЫМ ОСТЕОПЛАСТИЧЕСКИМ БИОКОМПОЗИЦИОННЫМ ПОКРЫТИЕМ ДЛЯ ВОССТАНОВЛЕНИЯ КОСТНОЙ ТКАНИ

Injuries of the musculoskeletal system, of different types of animals, occur in more than 30% of the total number of non-infected animals. In veterinary and humane traumatology, the problem associated with fractures of tubular bones has not been completely solved. Due to the use of different methods of osteosynthesis, implying the use of various implants (spokes, bone plates, osteofixers, pins), it is fundamentally important to obtain such a coating on implants that satisfies such specified needs as: thickness, morphological heterogeneity, roughness, porosity, adhesive strength to accelerate consolidation processes. The article shows the results of studies on the dynamics of hematological and biochemical parameters of groups of experimental animals, in view of the use of osteoplastic biocomposition coating for spokes in combined osteosynthesis of tubular bones. Blood samples (n=60) served as the material for research. The purpose of this study was to assess the dynamics of hematological and biochemical parameters of blood during the implantation of spokes with the developed osteoplastic biocomposition coating. A group of authors, based on the dynamics of hematological parameters, determined that the coating consisting of hydroxyapatite, methyluracil, amoxicillin and polylactide does not cause hematopoiesis inhibition and acute inflammatory reaction. Based on the dynamics of biochemical parameters, it was determined that this coating does not have hepatotoxicity and nephrotoxicity, the absence of a negative effect of this material on the process of bone restoration and the formation of a callus was determined. The developed coating, from the point of view of the authors, allows for a sufficiently high degree of consolidation of bone fragments and the exclusion of such a factor as inflammatory processes.

Complex effects of physical exertion with dietary supplements Cartilox in pain syndrome effectiveness and safety evaluation

To Identify the effect of physical activity complex effects with dietary supplements Cartilox (composition: hydrolyzed type II collagen, hyaluronic acid, boswellia, curcumin, piperine) on the severity of pain syndrome in OA knee and hip joint patients, low back pain (LBP); assessment of the need for the appointment of NSAIDs against the background of taking Cartilox. The study included 60 patients aged 35-65 years, with a confirmed diagnosis of knee and hip OA I-II st., LBP with a slight degree of severity of pain syndrome - 4-5 points on a numerical rating scale (NRS). Patients with comorbid diseases: arterial hypertension (AH), type 2 diabetes mellitus (DM-2), hypothyroidism, diseases of the gastrointestinal tract (gastrointestinal tract). By randomization, the patients were divided into two groups: Main group (n=30; 54.36±8.57 years) received a complex effect of non-drug therapy (physical therapy complex) with dietary supplements Cartilox 1 sachet per day during or immediately after meals for 1 month, in combination with non-medical therapy (physical therapy complex). And Control group (n=30; 53.03±16.18 years) used only non-medical therapy (physical therapy complex). In both groups, topical NSAIDs were used «on demand». The patients included in the study had imaging data of the spine and joints. Clinical and neurological examination was used: day 0 (Visit 1), Day 14 (Visit 2), Day 30 (Visit 3) of therapy. The dynamics of the condition was assessed: 10-point NRS of pain assessment (at rest, while walking, palpation), functional status of Oswestry Disability Index (ODI), blood pressure (BP) was measured, the dynamics of biochemical parameters (before and after 30 days) of blood glucose, liver enzymes (AST, ALT), weight indicators, registration of adverse events (AEs). A sub-objective assessment (1 to 5 balls) was given to the patient and the physician. Against the background of taking Cartilox, a statistically significant decrease in the severity of pain syndrome was noted, an improvement on ODI (to a greater extent in the Main group vs the Control group). In no case has a registered AEs. Changes in the level of biochemical blood parameters (glucose, liver enzymes) and blood pressure levels were not observed. The topical NSAIDs use was observed only in the Control group. The complex effect of physical exertion with dietary supplements Cartilox can be recommended for patients with unexpressed pain syndrome (4-5 points on the NRS) with LBP and knee and hip OA (I-II st.). The absence of changes in the level of biochemical parameters of blood and blood pressure makes it possible to recommend Cartilox to patients with OA and comorbid diseases.

АДАПТОГЕННОЕ ДЕЙСТВИЕ ПРЕПАРАТОВ АПИСИЛ И ТОС-БИО НА МЕДОНОСНУЮ ПЧЕЛУ В УСЛОВИЯХ ГИПЕРТЕРМИИ

The temperature factor has a huge impact on bees and their development. In a complex complex of adaptation, not only a perfect system of collective thermoregulation of the family is important, but also such individual adaptations as resistance to freezing and tolerance to high temperatures. The deviation of the ambient temperature from the optimal one increases the energy consumption of bees for the regulation of the microclimate in the family. This means that it enhances metabolic processes, which affects the state of the body and the life expectancy of insects. In addition, sometimes the use of high temperatures is a necessary condition for their maintenance, for example, in the fight against the parasite of the bee mite varroa. At the same time, the effect of high temperatures can have a negative effect not only on the mite, but also on the bees. In laboratory conditions, the effect of Apisil and TOS-BIO preparations containing 9-oxoand 10-hydroxy-2E-decenoic acids as a base - the most important components of the «queen substance» and royal jelly of the honey bee Apis mellifera L., respectively - on the survival rate and biochemical parameters was studied. adult worker bees exposed to extremely high (50oC) temperatures. The dynamics of the activity of phenoloxidase and catalase as biochemical indicators of the stress response of a honey bee under high temperature load has been studied. It was shown that the insects' resistance to hyperthermia increased with the content of these preparations in the feed. Experimental results indicate that under extreme (50oC) temperature conditions Apisil and TOS-BIO preparations have an adaptogenic effect on worker bees, increasing their resistance to hyperthermia. At the same time, changes in the initial level and dynamics of biochemical parameters indicate that these compositions in the most economical way contribute to the restructuring of metabolism in the mode of optimal functioning, which affects the level of changes in enzyme activity (with less pronounced changes in activity). and also on the survival rate of bees at the end of the experiment.

Питання терапії біліарної патології у дітей: вибір оптимального рішення

Актуальність. Захворювання гепатобіліарної системи — одна з найбільш поширених проблем педіатричної гастроентерології. Захворювання біліарного тракту (БТ) супроводжуються стійкими змінами обміну ліпідів, що сприяє тривалому холестазу і прогресуванню біліарної патології. Мета дослідження — вивчити ефективність застосування препарату Урсофальк® у комплексній терапії захворювань БТ і коморбідних станів у дітей. Матеріали та методи. У міському гастроентерологічному відділенні м. Харкова обстежено 55 дітей віком від 5 до 18 років (31 дівчинка та 24 хлопчика). Критерії включення пацієнтів у дослідження: захворювання БТ, що супроводжуються біліарним сладжем (БС) і порушенням ліпідного профілю (ЛП) сироватки крові. Діагностичний комплекс включав: аналіз клінічних даних, УЗД органів черевної порожнини, біохімічне дослідження сироватки крові («печінкові проби», ЛП). Усі діти отримували комплексне лікування з урахуванням коморбідної патології. Тривалість курсу терапії — 1 місяць. Пацієнти були розділені на дві групи залежно від жовчогінної терапії: 1-ша (основна) група отримувала традиційні жовчогінні засоби, 2-га (група порівняння) — препарат Урсофальк®. При статистичній обробці використані ліцензовані програмні продукти (Statistica, Excel). Результати. Клінічні прояви у дітей груп спостереження були представлені больовим і диспептичним синдромами. У всіх обстежених виявлено коморбідні стани і наявність БС до початку лікування. Після закінчення курсу лікування відзначалася позитивна динаміка клінічних симптомів, лабораторних показників і гомогенності структури жовчі. Застосування в комплексній терапії Урсофальку® дозволило повністю компенсувати відчуття тяжкості та іррадіацію болю, тоді як у дітей групи порівняння зберігалися больовий синдром, тяжкість у правому підребер’ї, диспептичні скарги. Слід зазначити, що застосування Урсофальку® дозволяє також нормалізувати структуру жовчі і біохімічні показники, що характеризують ліпідний обмін. Зареєстровано вірогідно (p < 0,05) більш виражений ефект зменшення частоти порушень гомогенності жовчі в основній групі — на 65,5 % порівняно з групою порівняння — 27,3 %. Позитивна динаміка біохімічних показників при контрольному обстеженні відзначалася в обох групах пацієнтів, при цьому вірогідно (p < 0,05) більш вираженими були зміни в групі дітей, які приймали Урсофальк®. Висновки. Застосування урсодезоксихолевої кислоти (УДХК) патогенетично обґрунтоване для корекції функціональних, метаболічних порушень при біліарній патології та коморбідних станах, що підтверджують результати спостереження. При призначенні Урсофальку® в комплексній терапії захворювань жовчовивідної системи у дітей відзначається швидка регресія і купіювання клінічних симптомів, нормалізація лабораторно-інструментальних показників. Урсофальк® є референтним препаратом УДХК. Наявність офіцінальної форми препарату Урсофальк® у спеціальній лікарській формі для дітей у вигляді суспензії дозволяє застосовувати УДХК в дитячому віці, починаючи з перших днів життя.

Dynamics of biochemical indicators in the process of mucous-bone wound healing in cases of spontaneous periodontitis on the background of different organism reactivity

The dynamics of biochemical parameters (lipid peroxidation, medium molecular peptides, prostaglandins) blood levels after periodontal tissues surgery of 24 dogs with spontaneous periodontitis has been studied. Animals were divided into groups with normal, hyper- and hyporeactivity of the body. It has been revealed that the changes of the studied biochemical parameters during normoreactivity of the organism are mutually correlated, which provides synchronization of necrotic and reparative processes in the healing of mucosal wounds after patch surgery in cases of spontaneous periodontitis. When hyperreactivity of organism is observed, the changes of indicators of lipid peroxidation, medium molecular peptides and prostaglandins promote strengthening of necrotic processes, and in cases of hyporeactivity they boost their slowing down that in both cases leads to delay of reparation in healing of an artificial mucous-bone wound. Keywords: spontaneous periodontitis, reactivity of the organism, wound healing, biochemical parameters.

Effectiveness of physical rehabilitation techniques with laser light in cardiovascular diseases under the control of a new diagnostic tools

Purpose . To study the effectiveness of laser therapy in patients with cardiovascular diseases using a diagnostic hardware-software complex. Material and methods . 120 patients with cardiovascular diseases (arterial hypertension – AH and ischemic heart disease – IHD) were taken into the study. Patients were divided into three groups depending on the rehabilitation program and prescribed basic medicamentous therapy: Group I – reflexotherapy (RT); Group II – RT and laser therapy (RT + LT); Group III (controls) – basic medicamentous treatment. At reflexotherapy sessions, biologically active points were electrostimulated with pointed pulses from «Lasper» device (Japan) having amplitude 0.8–2.5 V, pulse duration 1.5 ms, frequency 10 Hz and exposure 20 minutes. Infrared «Mlada» apparatus, generating in continuous mode with wavelength 0.85 μm, output power of two emitters 30 mW was used for laser therapy sessions. The effectiveness of treatment was assessed with diagnostic hardware and software complex (APK) «Physiocontrol-R» (Russia) which includes: analyzer of heart rate variability by cardiointervalogram, hemodynamic analyzer, psychological testing program (Spielberger, Beck, SAN test). The renin-angiotensin-aldosterone system (RAAS) and sympatho-adrenal system (SAS) were assessed with biochemical analyzer «Chemetrics' (USA). The developed calculation algorithms were used for assessing organism's functional reserves and risks of developing common diseases. Discriminant equations derived by us were used to assess the effectiveness of rehabilitation measures and to define groups of patients with varying level of effectiveness. Results . General mechanisms of RT and LT therapeutic effects in patients with cardiovascular diseases have been found out: correction of SAS and RAAS activity, limitation of excessive sympathetic impacts at heart and restoration of autonomic regulation function of the cardiovascular system, as a whole, which has been confirmed by the dynamics of biochemical parameters and SAS and RAAC decrease. Application of RT + LT complex for the rehabilitation of patients with hypertension and ischemic heart disease is more effective compared to RT monotherapy, which is confirmed by the largest increase in loading threshold (by 49.4%) when analyzing findings of the exercise test. Conclusion . 1. RT + LT complex applied in patients with cardiovascular diseases for rehabilitation is more effective modality in comparison with RT monotherapy. 2. The hardware-software complex «Physiocontrol-R» has been found to be a possible tool for evaluating the effectiveness of non-medicamentous therapy. 3. The developed diagnostic modality: algorithms for calculating a quantitative assessment of body's functional reserves and defining risks of common diseases as well as the discriminant equations for determining the level of effectiveness in different groups of patients – can be widely used for assessing the effectiveness of treatment.

The role of the gastrointestinal tract in the processes of intoxication and detoxification of the body

This review presents literature data on the role of the gastric tract in the processes of intoxication and detoxification of endoand exobiotics. The factors that promote the absorption and metabolism of toxic products in the gastrointestinal tract are described. The causes of intoxication and the mechanisms of two-phase detoxification with the participation of phase I enzymes of the biotransformation of xenobiotics in the intestine, in particular cytochrome P-450 isoenzymes, are described in detail. The predominance of the cytochrome P-450 isoenzyme CYP3A4 in the intestine was shown, and among the enzymes of phase II of biotransformation in the intestinal wall, the most important are UDP-glucuronyltransferase and sulfotransferase. The data on the ways of correction of intoxication with the use of a detoxification complex of dietary nutrition are presented. Clinical studies have shown that a specialized program provides antioxidant protection and the work of enzymes of the I and II stages of the metabolism of toxins, a decrease in the absorption and excretion of metabolic products, can significantly reduce the intoxication of the body, which is extremely necessary during the treatment and rehabilitation of diseases of the gastrointestinal tract and other organs and systems. rehabilitation of the body. It was found that the use of products of detox nutritional program LEOVIT DETOX led to a significant decrease in the concentration of urea and creatinine, which indicates an accelerated elimination of toxins and metabolic products formed in the body. A decrease in the concentration in the blood of the main enzymes characterizing the detoxification activity of the liver ALT, AST, GGT was also established.The aim of the study was to evaluate the detoxification effectiveness of the preventive nutrition Program and individual detoxification products LEOVIT DETOX, designed to affect all stages of Exo — and endobiotic metabolism.Materials and methods. Detoxification nutrition LEOVIT DETOX includes: specialized food product of dietary preventive nutrition “Complex nutrition program DETOX”, detoxifying jelly and bars — specialized food products of dietary preventive nutrition for detoxification of the body. The DETOX nutrition program is designed for 15 days and contains 30 products. The dynamics of biochemical parameters of liver and kidney function disorders (AST, ALT, GGT, urea, creatinine), blood lipid spectrum (by total cholesterol), fasting blood glucose level, and products of lipid peroxidation in blood serum were measured.Results. The use of specialized food products of dietary prophylactic nutrition “, a Comprehensive nutrition program DETOX” leads to a significant decrease in the concentration of urea and creatinine, AST, ALT, GGT, cholesterol, glucose, diene conjugate, ketodienes of CARBONYLS and increased total oxidative activity of blood serum were examined.Conclusion. The use of a specialized food product of dietary preventive nutrition “Complex nutrition program DETOX” provides effective detoxification of the body in diseases of the gastro-intestinal system.

Dynamics of biochemical parameters in lambs during the first four months of life

ABSTRACT We aimed to verify whether age influences the biochemical profile of healthy lambs during the first four months of life by characterizing the dynamics of its parameters and verifying whether there are differences between the profiles of growing animals and the reference values established for adults. Blood samples of 34 ½ White Dorper × ½ Suffolk female lambs were collected at 30, 60, 90, and 120 days of age, and the serum concentrations of total proteins, total globulins, albumin, [...]

Біохімічні показники життєдіяльності насіння гіркокаштанів, найпоширеніших у західному регіоні України

Визначено біохімічні показники життєдіяльності насіння гіркокаштанів для пошуку стійких проти каштанової мінуючої молі (Cameraria ohridella Desch. & Dem (Lepidoptera, Gracillariidaе)) видів і форм. Для експериментів у насінний рік в умовно чистих зонах і в місцях інтенсивного антропогенно-техногенного забруднення відібрано насіння чотирьох видів гіркокаштанів. Заготівлю насіння здійснено способом збирання із поверхні землі у період найінтенсивнішого його опадання. Досліджено вміст у насінні гіркокаштанів низки фізіолого-біологічних, морфологічних та основних біохімічних показників (білка, розчинних вуглеводнів і "сирого жиру"), золи, макро- і мікроелементів в умовно чистих зонах і в місцях інтенсивного антропогенно-техногенного забруднення. Встановлено рівень активності перекисного окислення ліпідів (ПОЛ) та вміст відновленого глутатіону. Вивчені зміни фізіолого-біологічних та біохімічних показників свідчать, що порівняно із трьома іншими досліджуваними видами дерева гіркокаштана кінського звичайного у стресових ґрунтово-кліматичних умовах великого міста з додатковим зниженням життєздатності каштановою мінуючою міллю характеризуються найбільшими негативними змінами у метаболізмі. Це свідчить, що за зростання в аналогічних ґрунтово-кліматичних умовах та за подібного навантаження чинників антропогенно-техногенного впливу, дерева і насадження жовтоквіткового (Aesculus octаndra Marsh.) і червоноквіткових видів гіркокаштанів (Aesculus pavia L. і Aesculus carnea Hayne) на сьогодні є життєво стійкішими, ніж дерева і насадження найпоширенішого у регіоні гіркокаштана кінського звичайного (Aesculus hippocastanum L.).

Динаміка біохімічних показників у пацієнток з клімактеричним синдромом на тлі цукрового діабету іі типу під впливом комплексної терапії із застосуванням препаратів Клімакт-хель та Мулімен

Динаміка біохімічних показників у пацієнток з клімактеричним синдромом на тлі цукрового діабету іі типу під впливом комплексної терапії із застосуванням препаратів Клімакт-хель та Мулімен

Biochemical profile of rats with non-alcoholic fatty liver disease of various gravity and its correction with Remaxol

Despite the long period of studying non-alcoholic fatty liver disease, its timely diagnosis, prevention and treatment remains one of the most pressing problems of medicine. However, the arsenal of effective and safe medicines used for this task is limited. The goal of this study was to elaborate a model of non-alcoholic fatty liver disease of varying severity in laboratory rats, and to analyze the influence of hepatoprotector medicine Remaxol upon the dynamics of biochemical parameters in experimental groups. The model of non-alcoholic fatty liver disease in laboratory rats permits to reproduce disease of va rying severity: semi–light severity of the disease (non-alcoholic hepatic steatosis) and average degree of severity (nonalcoholic steatohepatitis). The introduction of the test drug was carried out daily during 10 days of experiment starting from day 28th. Used model in experimental animals was characterized by the development of bilirubinemia, cholesterolemia (mainly due to triacylglycerides), activation of peroxidation, cytolytic and cholestatic syndromes. The severity of metabolic disturbances in the rats depended on the severity of the simulated disease. The analysis of the functional activity of the liver changes on the background of the application of Remaxol was performed for 11 parameters, yielding a complete picture of their condition. Distinct therapeutic effect of infusion of Remaxol on the model of liver steatosis (mild fatty liver) and in models of moderate severity (steatohepatitis), aimed at correcting observed violations was demonstrated in the study.

Biochemical Methane Potentials and Organic Matter Degradation of Swine Slurry under Mesophilic Anaerobic Digestion

Background/Objectives: Swine slurry is generally used as raw liquid fertilizer and leads environmental pollution. Therefore to overcome that, anaerobic digestion before its field application would reduce pollution and give bioenergy. Methods/Statistical Analysis: The dynamics of biochemical parameters in swine slurry are not fully evaluated in anaerobic system. In this study, basic changes in physico-biochemical character (pH, EC, solids, organic matters, nutrients, heavy metals, pathogens and methanogens) of swine slurry under mesophilic anaerobic digestion using batch system is evaluated. Findings: During mesophilic anaerobic digestion of swine slurry was shown the removal rate of organic matters at 85% and 75% in terms of BOD5 and SCODCr. The pathogens of Salmonella and fecal coliforms removed at 100% and 97%. Interestingly, the nutrients contents were increased at 19% and 12% in terms of NH3-N and available phosphorous, respectively. The biochemical methane potentials of swine slurry was observed at 236 L/kg CODadded, and 307 L/kg VSadded, respectively. The methane accounted for 54.3% of the biogas produced with the dominant population of Methanosarcina sp. Conclusion/Improvements: It is concluded that the mesophilic anaerobic digester is greatly desirable for swine slurry with regards of bioenergy, ecofriently liquid biofertilizer production and significant biodegradability of organic waste.

Динамика биохимических показателей грануляционной ткани экспериментальных инфицированных ран при лечении биологически активными препаратами

Динамика биохимических показателей грануляционной ткани экспериментальных инфицированных ран при лечении биологически активными препаратами

Dynamics of some biochemical parameters in patients with peripheral occlusive arterial disease at different periods following reconstructive surgery

According to various authors and our own experience, intimal hyperplasia develops in 40-50% cases following reconstructive surgeries causing complications in post-operative period by the development of restenosis. Nowadays more importance in the development of intimal hyperplasia is given to the dynamics of different biochemical parameters of blood plasma. This work represents the analysis of blood plasma endothelin-1, adhesion molecules and C-reactive protein rates in patients with peripheral occlusive arterial disease at different periods following reconstructive surgery. Research was performed in 25 male patients with peripheral occlusive arterial disease stage IIb-IV according to Fontaine-Pokrovsky classification who underwent reconstructive surgery with the use of allo-prostesis.

The Study of Effects of Toxicants and Heliophysical Factors on Biochemical Parameters of External Mucus in Carp

Dynamics of biochemical parameters of external mucus of the carp Cyprinus carpio L. exposed to 5 mg/l and 10 mg/l concentrations of phenol was studied during the exposure period and after it. Biochemical parameters of external mucus (specific weight, pH, total protein, haemoglobin, ketones) were measured 1, 3, 6, 24 hrs after the initiation of exposure and regularly during the 21-day postexposure period, when fish were transferred into fresh water. Significant changes in haemoglobin and total protein concentrations were determined during the period of exposure to phenol and after it. Correlations between changes in biochemical parameters of mucus and the intensity of some parameters of solar activity and radio radiation flow were ascertained during the post-exposure period. The conclusion was made that it is necessary to assess the possible combined effect produced by environmental factors of various origin and magnitude on the fish organism.