Distillers Dried Grains With Solubles Research Articles

Effects of branched-chain amino acid imbalance and dietary valine and isoleucine supplementation in modified corn-soybean meal diets with corn distillers dried grains with solubles on growth performance, carcass quality, intestinal health, and cecal microbiome in Cobb 500

One important feature of corn distillers dried grains with solubles (DDGS) is its high leucine:lysine ratio, which can inhibit chicken growth by causing branched-chain amino acid (BCAA) antagonism. The current study was conducted to investigate the effects of BCAA imbalance of inclusion of DDGS and whether additional dietary valine and isoleucine could alleviate the negative effects in broilers. A total of 640 0-d-old male Cobb 500 broilers were allocated into 4 treatments with 8 replicates and reared until d 42. The four different dietary groups were as follows: 1) control (CON) group (corn-soybean meal-based diet); 2) 30% DDGS (30D) group (replacing soybean meal with 30% DDGS); 3) 30D + additional valine and isoleucine (30DB) group; and 4) the group of 30DB + additional valine and isoleucine to provide the same leucine:valine and leucine:isoleucine ratios as the CON group (30DBB). The analyzed leucine:lysine ratios of the CON group were 1.36/1.41/1.46 (starter/grower/finisher phase), whereas the average leucine:lysine ratios of the 30% DDGS groups were 1.61/1.70/1.78 (starter/grower/finisher phase). The 30% DDGS groups (30D, 30DB, and 30DBB) negatively affected body weight (BW) from d 7 to 42 and BW gain (BWG), feed intake, carcass weight, breast muscle weight, and jejunal and ileal villus height:crypt depth during the overall period (d 0 to 42) (P < 0.05). Furthermore, the 30% DDGS groups significantly altered expression levels of jejunal tight junction proteins, breast muscle mechanistic target of rapamycin (mTOR) pathway-related genes, BCAA catabolism genes, and AA transporters compared to the CON (P < 0.01). The 30% DDGS groups showed differences in beta-diversity indices compared to the CON group (P < 0.05). The 30DBB group showing the lowest d 21 and 42 BW and overall BWG had the largest differences compared to the CON group in most measurements. In conclusion, excessive replacement of soybean meal with DDGS can significantly increase leucine levels, which may negatively affect chicken growth. Additionally, inappropriate ratios of valine and isoleucine can further decrease growth performance.

Effect of temperature on the digestibility of non-starch polysaccharide-rich ingredients in Nile tilapia (Oreochromis niloticus)

This study aimed to investigate the interaction effect of NSP-rich ingredient and rearing temperature on nutrient digestibility of the ingredients in Nile tilapia. Seven NSP-rich ingredients were tested: insect meal, wheat bran, palm kernel meal, rapeseed meal, sunflower meal, dried distillers grains with solubles (DDGS) from corn, and rice bran. A balanced control diet was formulated followed by the formulation of seven test diets by mixing 30 % of each test ingredient with 70 % of the control diet and fed to fish reared at 24 or 32 °C. At each temperature, 35 all male Nile tilapia with an initial mean weight of 45.0 g at 24 °C and 43.8 g at 32 °C were housed in each of 24 glass-tanks, each containing 60 L of water. The diets were administered to the fish groups in triplicate over 42 d in a recirculating aquaculture system. Fecal samples were collected and analyzed alongside the diets to determine nutrient digestibility. Digestibility of all nutrients differed between ingredients (P < 0.001) at both temperatures. Wheat bran had the lowest NSP digestibility at both 24 (1.9 %) and 32 °C (9.7 %). At 24 °C, DDGS showed the highest NSP and protein digestibility (41.4 and 90.3 %), while at 32 °C insect meal showed the highest NSP and fat digestibility (59.8 and 97.5 %). Insect meal showed the highest fat digestibility (97.4 %) also at 24 °C. An interaction effect between ingredient and temperature was observed for the digestibility of NSP, carbohydrate, protein, amino acids, magnesium, and dry matter (P ≤ 0.035). Some nutrients showed reduced digestibility for some ingredients at the higher temperature, but in most of the cases temperature increased nutrient digestibility, with insect meal exhibiting a 20 % increase in NSP digestibility. In conclusion, water temperature affect nutrient digestibility, but the extend of the temperature effect differs between ingredients.

Preparation of metal-modified carbon-based catalyst and experimental study on catalytic pyrolysis of distillers dried grains with solubles

Distillers dried grains with solubles (DDGS) offer high calorific value, suited for catalytic rapid pyrolysis for energy and chemical applications, yet tar and coke formation during bio-oil upgrading necessitates exploration of cost-effective, durable biocarbon-based catalysts for tar removal. In this study, a carbon-based catalyst was prepared by metal modification of alkaline biochar for catalytic pyrolysis with Distillers dried grains with solubles (DDGS) biomass. Brunauer–Emmet–Teller (BET), X-ray diffraction (XRD), Fourier Transform Infrared (FTIR) were used to characterized the morphology and microstructure of the metal-modified carbon-based catalysts. Pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) was used to analysis the pyrolysis-gas of catalytic pyrolysis. Furthermore, the effects of the loading and metal mass ratio of carbon-based monometallic catalysts (Fe and Co) and carbon-based bimetallic catalysts (Fe-Co) on the distribution of the DDGS pyrolysis products were further investigated. The results showed that the 8 wt% of loading rate of bimetallic catalyst significantly reduced the oxygenated compounds but increased the aromatic hydrocarbons. Compared with pyrolysis without catalyst, when the catalyst was 2Fe6Co (mass ratio of Fe/Co 1:3), the hydrocarbons increased from 36.42 % to 44.53 %, the oxygen-containing compounds decreased from 60.36 % to 52.35 %, and the aromatics increased significantly from 0.73 % to 25.37 %. This study provides a new route of increasing the aromatic hydrocarbon content of catalytic pyrolysis to offer theoretical basis for carbon-based catalysts of biomass conversion.

43 Approaches for improving nitrogen utilization efficiency and environmental sustainability of pork production systems

Abstract Nitrogen (N) is an essential element for life, but due to its inefficient utilization, N waste and emissions generated have exceeded the planetary boundaries and ability of the Earth to effectively overcome these environmentally detrimental effects. Global food animal production contributes 29 to 34% of total global N emissions, with pork supply chains contributing about 16% of these emissions. Life cycle N use efficiency (NUE) of U.S. pork production is only about 56%, which is similar to broiler (55%) and egg (50%) production, but greater than beef (10%) and milk (31%) production. However, only about 10 to 44% of dietary N is converted into edible pork products. While genetic improvement has increased NUE associated with improved lean growth rate, improvement in litter size has led to an increased proportion of low-birth-weight pigs that have reduced NUE. Although metabolic modifiers including porcine somatotropin, ractopamine, and immunocastration improve NUE, they are not feasible or acceptable to consumers in many countries. Separate sex feeding and reducing market weight can also improve NUE. Ultimately feeding program design and implementation have the greatest potential to improve NUE because feed is associated with 70% of N emissions in pork production systems. Multi-objective feed formulation using Life Cycle Assessment environmental impact data of feed ingredients, precision feed formulation and feeding practices to overcome variability in digestible nutrient content of feed ingredients and nutritional requirements of pigs fed in groups, feed processing to enhance energy and nutrient digestibility, and minimizing feed wastage offer the greatest opportunity to improve NUE and environmental sustainability. There are substantial differences in greenhouse gas emissions and embedded water and land use associated with growing-finishing feeding programs depending on the dietary proportions of corn, soybean meal, distillers dried grains with solubles (DDGS), and crystalline amino acids among U.S. geographic regions. Furthermore, addition of thermally processed supermarket food waste to corn-soybean meal (CSBM) diets can significantly reduce environmental impacts and diet cost without compromising growth performance and carcass composition compared with current commercial feeding programs. Although growing-finishing feeding programs using CSBM diets with minimal crystalline amino acids result in greater N intake, N retained, and urinary N excretion compared with feeding low protein, crystalline amino acid supplemented (LP) diets, and diets containing 30% DDGS, growth performance and carcass composition are optimized and impacts on climate change, marine and freshwater eutrophication, and fossil resource are reduced by feeding CSBM diets compared with feeding LP or DDGS diets. Sourcing and using feed ingredients with reduced environmental impact metrics along with precision diet formulation and feeding practices to ensure “getting the right amount of energy and nutrients in the right feed fed to the right pigs at the right time” can improve NUE and environmental sustainability of pork production systems.

PSVI-23 Economic potential of field peas as an alternative to corn distillers dried grain with solubles (DDGS) in beef heifer growing diets

PSVI-23 Economic potential of field peas as an alternative to corn distillers dried grain with solubles (DDGS) in beef heifer growing diets

Effects of high-level dietary distillers dried grains with solubles supplemented with multienzymes on growth performance, nutrient utilization, intestinal morphology, and pellet quality in broiler chickens.

With the increasing cost of bulk raw materials and advancements in the feed enzyme industry, corn distillers dried grains with solubles (DDGS) have shown more opportunities for use in broiler diets. Supplementation with multiple enzymes could mitigate anti-nutritional factors in DDGS, enhance nutrient digestibility, and thereby increase its utilization in broiler diets, leading to reduced feed costs. This study evaluated the effects of multienzyme supplementation on growth performance, nutrient utilization, intestinal morphology, and pellet quality in broiler chickens fed diets containing conventional levels of DDGS (C-DDGS) and higher levels of DDGS (H-DDGS). A total of 800 1-day-old Cobb 500 chicks was assigned to four dietary treatments with eight replicates of 25 birds each: C-DDGS (5% DDGS in Starter and 10% in Grower), C-DDGS + Enzyme (C-DDGS diet supplemented with multienzyme), H-DDGS (10% and 20%) + Enzyme (H-DDGS diet supplemented with multienzyme, 10% DDGS in Starter and 20% in Grower), and H-DDGS (15% and 30%) + Enzyme. The C-DDGS + enzyme diet increased (p < 0.05) body weight gain (BWG), reduced the feed conversion ratio, enhanced (p < 0.05) digestibility of dry matter (DM), crude protein, and hemicellulose (HC), and improved (p < 0.05) intestinal villus height and villus: crypt ratio of broilers. The H-DDGS (10% and 20%) + enzyme diet exhibited no difference in (p > 0.05) growth performance, nutrient digestibility (except HC), and intestinal morphological parameters, whereas the H-DDGS (15% and 30%) + enzyme diet decreased (p < 0.05) feed intake and BWG and reduced (p < 0.05) energy and DM digestibility by impact (p < 0.05) intestinal morphology compared with the C-DDGS enzyme-free diet. The H-DDGS diet had lower (p < 0.05) pellet hardness and poorer durability than the C-DDGS diet. Supplementing multienzyme in the C-DDGS (5% and 10%) diet improved growth performance from day 0 to 28 and diminished growth performance in the H-DDGS (15% and 30%) diet by influencing intestinal morphology and feed pellet quality in broiler chickens. In addition, when supplemented with multienzyme, the dietary DDGS level can be safely included at levels of 10% in 0-7 days and 20% in 8-28 days of age.

Amino acid and energy digestibility of conventional corn dried distillers grains with solubles, high protein distillers dried grains, and corn fermented protein, and impacts on turkey poult growth performance and intestinal characteristics

Amino acids (AA) are an expensive nutritional components of poultry diets. Distillers dried grains with solubles (DDGS) is the primary co-product produced by the dry grind bioethanol industry, although new technologies are being implemented to produce high protein distillers dried grains (HP-DDG) and corn fermented protein (CFP), but data on their nutritive value in poultry are lacking. Two experiments (EXP) were conducted to determine the energy and AA digestibility of DDGS, HP-DDG, and CFP in poults in addition to a feeding trial to evaluate increasing dietary levels of HP-DDG and CFP on growth performance and intestinal characteristics. In EXP 1, six different DDGS sources were evaluated using poults to determine their nitrogen-corrected apparent metabolizable energy (AMEn) concentrations, and cecectomized roosters were used to determine their standardized ileal (SID) AA digestibility (SID-AA). In EXP 2, AMEn and SID-AA for HP-DDG and CFP were determined in young poults, and a feeding trial was conducted to evaluate growth performance and intestinal morphology and permeability of poults fed diets containing 7.5 and 15% HP-DDG or CFP from 1 to 32 d of age. In EXP 1, the AMEn concentration among the DDGS samples ranged from 2,530 to 3,573 kcal/kg DM but was not different (P = 0.57) among the samples, with an average SID for LYS of 66.6%. In EXP 2, different (P = 0.001) AMEn concentrations for HP-DDG and CFP were observed (3,114 and 3,760 kcal/kg DM, respectively), with the SID for LYS being 66.55 and 77.00% for HP-DDG and CFP, respectively. Including HP-DDG or CFP into the diet at 7.5 and 15% had no effect (P > 0.05) on growth, feed intake, or feed conversion. Neither co-product nor its inclusion rate affected intestinal morphology and permeability (P > 0.05). Overall, DDGS, HP-DDG, and CFP are excellent sources of AMEn and digestible AA, with dietary inclusion rates of up to 15% of HP-DDG or CFP having no impact on growth or intestinal characteristics.

Possibilities of Inclusion of corn Distillers Dried Grains with Solubles (DDGS) alone or in combination with Probiotic as Broiler Feed Ingredient

In the production of the ethanol from cereal grains, besides the main product which is ethanol, there are olso very valuable by-product that can be used as animal feed. The dried distilled grains with solubles (DDGS) is quite rich in protein and other nutrients. With the ever-increasing cost of soybeans and corn, many scientists are studying the possibilities of using DDGS in poultry nutrition. The aim of the present study was to determine the effect of including corn DDGS and corn DDGS and probiotic in compound feeds on productive, slaughter performance and some meat physicochemical parameters of broiler chickens under floor-rearing conditions. The experiment included 3 treatments (T), namely T1: the control group received a balanced compound feed, according to the requirements of the hybrid, without the participation of corn DDGS, T2: the diett contained 15% corn DDGS and T3: the dietts contained 15% corn DDGS and supplemented 500g probiotic/ton of feed. Each experiment had 20 one-day-old sexed male Ross-308 broiler chickens, replicated 3 times, and ended when the chickens were 35 days old. The results shown that diets T2 and T3 did not positively affect the weight development of chicks compared to T1 (the control group). At 35 days old, chicken weight from the control group outperformed the two experimental groups: by 8.45% in the T3 (corn DDGS and probiotic) and by 11.76% in the T2 (corn DDGS) at P&lt;0.05. Differences in feed consumption per 1 kg of growth over the 35-day fattening period between the control and experimental groups were small and statistically unproven. Feeding diets with 15% DDGS from corn significantly reduced carcass weight, grill, thight and wing weight in broiler chickens. There were no significant differences in slaughter yield between the study groups. Diets T2 and T3 improved the water holding capacity (WHC %) of breast meat in broilers (at P&lt; 0.001). The lowest loss in thermal processing (cooking) was observed in breast and thigh meat of chickens fed the diets with 15% corn DDGS and probiotic (P&lt;0.05).

Determination of phytic acid disappearance, ileal P digestibility at different dietary Ca levels, and relative P bioavailability in soybean meal, canola meal, distillers dried grains with solubles, corn fermented protein, and wheat middlings

Three experiments were conducted to evaluate P utilization in soybean meal (SBM), canola meal (CM), distillers dried grains with solubles (DDGS), corn fermented protein (CFP), and wheat middlings (WM) using different assays. In Experiment 1, phytic acid disappearance (myo-inositol 1,2,3,4,5,6-hexakis; InsP6D) and inositol phosphate disappearance (InsP-PD) were determined using precision-fed cecectomized Leghorn roosters. Roosters were precision-fed 20 to 25 g of SBM, CM, DDGS, CFP, and WM. In Experiment 2, InsP6D, InsP-PD, and standardized ileal digestibility (SID) of P at different Ca levels were determined using ad libitum-fed broiler chickens. Semi-purified cornstarch-dextrose-based diets containing SBM, CM, DDGS, CFP, and WM as the sole source of P were fed. All diets contained 0.21% P and limestone was added at the expense of dextrose to provide 0.30, 0.45, 0.60, and 0.75% Ca. In Experiment 3, P bioavailability relative to KH2PO4 was determined based on tibia bone ash. Experiments contained 5 to 6 replicates per treatment. In Experiment 1 with precision-fed roosters, InsP6D and InsP-PD ranged from 8 to 71% among feedstuffs, with the lowest (P < 0.05) disappearance being observed in SBM. In Experiment 2 with ad libitum-fed chickens, there was a Ca × ingredient interaction (P < 0.05) whereby increasing Ca linearly decreased (P < 0.05) InsP6D, InsP-PD, and SID of P for all feedstuffs, excluding CFP. Estimated P digestibility calculated using InsP6D in Experiment 1 was in good agreement with SID in Experiment 2 determined at 0.75% Ca, except for SBM. In Experiment 3, regression of bone ash content (mg/tibia) on supplemental P intake yielded P bioavailability values ranging from 30 to 81% among feedstuffs relative to KH2PO4, with the highest (P < 0.05) bioavailability being observed for DDGS and CFP. In conclusion, 1) InsP6D in precision-fed roosters can provide preliminary indications of P digestibility in plant-based feedstuffs, 2) SID determined at 0.75% Ca was in good agreement with other bioassays, and 3) P in DDGS and CFP was highly available compared with other feedstuffs.

Advances in Extraction, Structure, and Physiochemical Properties of Sorghum Kafirin for Biomaterial Applications: A Review.

Kafirin is an endosperm-specific hydrophobic protein found in sorghum grain and the waste by-product from sorghum biorefineries known as sorghum dried distillers' grain with solubles (DDGS). Because of kafirin's poor nutritional profile (negative nitrogen balance, slow digestibility, and lack of some essential amino acids), its direct human use as a food is restricted. Nevertheless, increased focus on biofuel production from sorghum grain has triggered a new wave of research to use sorghum DDGS kafirin as a food-grade protein for biomaterials with diverse applications. These applications result from kafirin's unique chemical nature: high hydrophobicity, evaporation-induced self-assembling capacity, elongated conformation, water insolubility, and low digestibility. Aqueous alcohol mixtures have been widely used for the extraction of kafirin. The composition, structure, extraction methodologies, and physiochemical properties of kafirin, emphasising its biomaterial functionality, are discussed in detail in this review. The literature survey reveals an in-depth understanding of extraction methodologies and their impact on structure functionality, which could assist in formulating materials of kafirin at a commercial scale. Ongoing research continues to explore the potential of kafirin and optimise its utilisation as a functional biomaterial, highlighting its valuable structural and physicochemical properties. Further studies should focus on covering gaps in the research as some of the current structural understanding comes from data on zein protein from maize.

Improving Biodegradable Films from Corn Bran Arabinoxylan for Hydrophobic Material and Green Food Packaging.

Non-biodegradable plastic materials pose environmental hazards and contribute to pollution. Arabinoxylan (AX) films have been created for applications in food packaging to replace these materials. The water interaction characteristics of biodegradable AX films were assessed following the extraction of AX from dry-milled corn bran (DCB), wet-milled corn bran (WCB), and dried distiller's grains with solubles (DDGS). Films were prepared with laccase and sorbitol before surface modification with lipase-vinyl acetate. Water solubility of the modified DCB films was significantly reduced (p < 0.05); however, the water solubility of modified WCB films decreased insignificantly (p > 0.05) compared to unmodified films. Water vapor permeability of the modified AX films from WCB and DDGS was significantly reduced (p < 0.05), unlike their unmodified counterparts. The biodegradation rates of the modified WCB AX and DDGS films increased after 63 and 99 days, respectively, compared to the unmodified films. The hydrophilic nature of AX polymers from WCB and DDGS enhances the biodegradability of the films. This study found that the modified WCB AX film was more hydrophobic, and the modified DDGS AX film was more biodegradable than the modified DCB AX film. Overall, surface modifications have potential for improving hydrophobicity of biopolymer films.

A new spectral simulating method based on near-infrared hyperspectral imaging for evaluation of antibiotic mycelia residues in protein feeds

Antibiotic mycelia residues (AMRs) contain antibiotic residues. If AMRs are ingested in excess by livestock, it may cause health problems. To address the current problem of unknown pixel-scale adulteration concentration in NIR-HSI, this paper innovatively proposes a new spectral simulation method for the evaluation of AMRs in protein feeds. Four common protein feeds (soybean meal (SM), distillers dried grains with solubles (DDGS), cottonseed meal (CM), and nucleotide residue (NR)) and oxytetracycline residue (OR) were selected as study materials. The first step of the method is to simulate the spectra of pixels with different adulteration concentrations using a linear mixing model (LMM). Then, a pixel-scale OR quantitative model was developed based on the simulated pixel spectra combined with local PLS based on global PLS scores (LPLS-S) (which solves the problem of nonlinear distribution of the prediction results due to the 0%-100% content of the correction set). Finally, the model was used to quantitatively predict the OR content of each pixel in hyperspectral image. The average value of each pixel was calculated as the OR content of that sample. The implementation of this method can effectively overcome the inability of PLS-DA to achieve qualitative identification of OR in 2%-20% adulterated samples. In compared to the PLS model built by averaging the spectra over the region of interest, this method utilizes the precise information of each pixel, thereby enhancing the accuracy of the detection of adulterated samples. The results demonstrate that the combination of the method of simulated spectroscopy and LPLS-S provides a novel method for the detection and analysis of illegal feed additives by NIR-HSI.

Inclusion of Lonicerae flos improved anaerobic fermentation and antioxidant activity of mixed silage (agro-residue and alfalfa)

Lonicerae flos (SY) is rich in active components including chlorogenic acid, flavonoids, phenols and terpenoids, with antioxidant and antibacterial activities. The present study was designed to evaluate the application potential of SY in silages. Various concentrations of SY (0, 5, 10, 15, 20, and 25 g/kg of fresh weight [FW]) were added to a mixture of alfalfa, distillers dried grains with solubles (DDGS) and soybean meal (80/10/10). All mixtures were ensiled for 30 d in laboratory silos, the experiment followed a completely randomized design, and six treatments were designed according to SY levels, with 5 replicates per treatment. Data were subjected to one-way analysis of variance and polynomial contrasts (SPSS 23.0) to examine effects of varying concentrations of SY. Adding SY enriched relative abundance of Lactobacillus acetotolerans, increased dry matter (DM), water soluble carbohydrate (WSC), chlorogenic acid, total phenolics content, and ferric reducing antioxidant power (FRAP) reducing power, and reduced pH, ammonia nitrogen (AN) and structural carbohydrates (NDF, ADF, and hemicellulose) contents. L. acetotolerans was positively correlated with WSC, DM, chlorogenic acid, total phenolics, FRAP, and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid diammonium salt radical cation (ABTS) concentrations, while negatively correlated with pH, AN and structural carbohydrates contents. Base on the predicted pathways and phenotype of the bacterial community, the addition of SY promoted the genetic information processing (translation, replication and repair, transcription), and inhibited the abundances of potentially pathogenic, stress tolerant and gram negative. Silages with SY at 20 g/kg FW (SY4) or 25 g/kg FW (SY5) had lower pH, contents of AN and structural carbohydrates than other silages. The chlorogenic acid and total phenolics content in SY3 and SY4 were higher than other silages, while FRAP reducing power and ABTS scavenging activity in SY3 (15 g/kg FW) and SY4 silage was highest, respectively. Overall, the inclusion of SY at 20 g/kg FW decreased pH and AN content, more chlorogenic acid, increased the relative abundance of L. acetotolerans and antioxidant activity, with the potential to serve as a feed additive.

50 Feeding a multi-carbohydrase improves mortality, morbidity and variability of growing pigs fed varying levels of dried distiller’s grains with solubles

Abstract The objective was to determine the effects of Rovabio Advance 10 P, a multi-carbohydrase, on growth performance, mortality, and morbidity of growing pigs fed 15% or 35% dried distiller’s grains with solubles (DDGS). In the trial, 1,220 DNA pigs were fed from 32 kg to 134 kg body weight (BW). Four diets were fed: 15% DDGS (LO), 15% DDGS with Rovabio Advance (LO+), 35% DDGS (HI), and 35% DDGS with Rovabio Advance (HI+). Each pen had approximately 30 pigs each with 10 pens per dietary treatment. Pig BW and feed were measured as were the number and BW of dead pigs (mortality) and other pig removals (morbidity). Pigs were harvested beginning on d 105 and weekly thereafter through d 126. No significant (P &amp;gt; 0.16) interactions between DDGS level and Rovabio Advance were observed. Feeding greater levels of DDGS tended to reduce average daily gain (ADG), final BW and carcass weight (P &amp;lt; 0.09) and reduced mortality and morbidity (P = 0.1). Greater levels of DDGS also reduced cost of gain and improved income over feed (P &amp;lt; 0.04). Feeding Rovabio Advance reduced mortality and morbidity (P = 0.08), reduced standard deviation of carcass weight (P = 0.02), improved carcass price (P = 0.02), and improved income over feed (P = 0.02). Feeding greater levels DDGS slowed growth rate but improved cost of gain and profitability. Rovabio Advance improved mortality and morbidity and reduced variability resulting in improved profitability.

172 Effects of post-MSC DDGS inclusion in gestation diets on total tract nutrient and energy digestibility compared with other fiber sources

Abstract The high fiber content of corn dried distiller grains with solubles (DDGS) make it an ideal candidate for inclusion in gestation sow diets. Gestating sows, however, have greater utilization of fibrous ingredients, and nutrient values attained in grower pigs are not representative of nutrient metrics for gestating sows. The objective of this study was to evaluate apparent total tract digestibility (ATTD) of energy and nutrients from post-protein separation (post-MSC) DDGS provided to gestating sows in comparison with soyhulls and sugar beet pulp; two commonly used fiber sources in gestation diets. Pregnant sows [n = 22; parity 0 to 1; initial body weight (BW) = 190 ± 10 kg] were allocated to 1 of 6 dietary treatments in a crossover design with two collection periods (period 1 from d 40-59 of gestation and period 2 from d 60-79 of gestation) to attain at least 7 observations per treatment. Two of the six diets were practical corn-soybean meal-based gestation diets with either soyhulls (17.5% inclusion) or post-MSC DDGS (15% inclusion) to determine ATTD of energy and nutrients in complete diets. The remaining four diets included a corn-basal diet and the basal diet plus 30% inclusion of post-MSC DDGS, soyhulls, or sugar beet pulp. Titanium dioxide was included as an indigestible marker to calculate ATTD. Sows were fed experimental diets (2.2 kg/d in a single feeding) in two 20-d collection periods where the initial 14 d considered a diet adaptation period followed by 5 d for total collection of urine (collected using urine catheters inserted on d 14) and grab fecal samples. Energy values for the post-MSC DDGS, soyhulls, and sugar beet pulp in the corn-basal diets were calculated using differential procedure. Data were analyzed with ANOVA and fixed to a mixed linear model using treatment as the main effect and collection period as a random variable. The ATTD of the post-MSC DDGS practical diet was 8% greater in ATTD of crude protein and 13% greater in ATTD of ether extract than the practical soyhull diet (P &amp;lt; 0.01). The ATTD of gross energy of post-MSC DDGS was 16% greater than soyhulls (P &amp;lt; 0.01). This resulted in an 800 and 750 kcal/kg greater digestible energy and metabolizable energy content, respectively on an as-fed basis in post-MSC DDGS compared with soyhulls and sugar beet pulp (P &amp;lt; 0.01). The post-MSC DDGS also had a net energy value (as-fed basis) 700 kcal/kg greater than soyhulls and sugar beet pulp. In conclusion, post-MSC DDGS is suitable for inclusion in gestating sow diets as a fiber source while meeting the energy needs of the gestating sow.

PSIV-21 Ileal digestibility of amino acids in feed ingredients for 22-, 56-, and 87-kilogram pigs

Abstract The objective of the present study was to test the hypothesis that the apparent ileal digestibility (AID) of amino acids (AA) in feed ingredients is not constant in pigs with 3 different body weights (BW). Eight pigs with initial BW of 22.2 ± 1.2 kg cannulated at the distal ileum were used. Two experimental diets contained corn distillers dried grains with solubles (DDGS) and soybean meal-wheat mixture (SBM-W; 70% soybean meal + 30% wheat) as the sole source of nitrogen, respectively. The diets were assigned to the animals in a 2-period cross-over design. Each period consisted of a 5-d adaptation period and a 2-d ileal digesta collection period. The animals, diets, and experimental design for 50 kg pigs (initial BW = 55.6 ± 3.3 kg) and 80 kg pigs (initial BW = 86.9 ± 5.1 kg) were identical to those used for 22 kg pigs. Orthogonal polynomial contrasts were used to determine the effect of ingredient type (corn DDGS or SBMM) and BW of pigs (from 22 to 87 kg) and the interaction between these 2 main effects. The AID of crude protein, lysine, and methionine in both corn DDGS and SBM-W linearly or quadratically increased (P &amp;lt; 0.05) with an increase in pig BW. An interaction between ingredient type and linear effect of BW was observed (P &amp;lt; 0.05) in histidine, isoleucine, leucine, phenylalanine, threonine, and valine. Specifically, a linear increase in AID of these AA with increasing BW of pigs was greater in SBM-W compared with corn DDGS. In conclusion, the AID values of most AA differed between the BW range of 22 to 87 kg, and these patterns varied among different ingredients.

283 Identification of new or existing risk factors associated with the development of sow pelvic organ prolapse

Abstract A survey was conducted between March and December of 2022 to examine the variation between farms that had high and low pelvic organ prolapse (POP) rate. The objective was to identify new or existing risk factors associated with the development of POP, which could open new avenues for further investigation. Sow farms (n = 53) across 26 production systems located in the US, Canada, and Mexico were used. Sampling of farms was not random. High and low POP systems were selected within each region as well as high and low POP farms within each system. Over 800 variables related to general farm information and management, performance traits, reproductive management, replacement gilt management, gestation and farrowing practices, labor and health management, and nutrition were collected during farm visits. To be included in the analysis, at least 70% of observations were needed for continuous variables with at least 10% of observation for each category for categorical variables. Univariable models were fitted to identify variables associated with annualized POP rate for 2021. Variables with P ≤ 0.10 were selected for further analysis, with 94 predictor variables selected for principal components analysis (PCA). A factor analysis of mixed data was conducted, and results were then fitted into a hierarchical clustering on PCA, which segregated the data into three clusters: 2 with low POP (2.5%) and 1 with high POP (6.1%) prevalence (low vs high P &amp;lt; 0.05). The resulting clusters explained 33.7% of the variability in annualized 2021 POP rate. Differences between clusters were evaluated by Kruskall-Wallis test for numerical variables and chi-square test for categorical variables. High POP cluster farms had greater total piglets born over the years, greater number of sows per worker, greater annual gestation feed usage, and a lower mean body condition score at 16 wk of gestation compared with low POP cluster farms. High POP cluster farms had a decreased proportion of farms using conventional artificial insemination, a greater proportion of farms utilizing cup waterers in gestation for sows in group housing and in farrowing, and greater proportion of farms with a routine sleeving practice. High POP cluster farms compared with low POP cluster farms had greater gestation distillers dried grains with solubles (DDGS) inclusion level (14.6% vs 4.0%, SE 3.02), greater levels of standardized total tract digestibility (STTD) P% (0.49% vs 0.39%, SE 0.014), narrower analyzed Ca to STTD P ratio (1.7 vs 2.2, SE 0.06), and lower phytase inclusion and release (0.16% vs 0.09%, SE 0.014). These variables were also the predictors with the greatest R2 values in the univariable models. These observations suggest further research is needed to evaluate the connection between dietary Ca and P and pelvic organ prolapse.

278 Phytogenic feed additive increased sow lactation average daily feed intake, litter weaning weight, and subsequent conception rate when fed in summer environmental conditions

Abstract An experiment was conducted to test the hypothesis that feeding a phytogenic feed additive (Fresta F, Delacon, Austria) to sows during lactation would increase lactation average daily feed intake (ADFI) during natural heat stress from summer environmental conditions. At the time of entry to lactation facilities, 192 sows were randomly allotted by parity and within farrowing batch to one of two treatments. A basal lactation diet based primarily on corn, soybean meal, and dried distiller’s grains with solubles (DDGS) served as the control treatment, whereas the test treatment contained 0.04% phytogenic feed additive at the expense of corn. Before farrowing, sows were limit fed, but once farrowing occurred, sows were provided with ad libitum access to feed. Individual feed disappearance was recorded daily, and sow was considered the experimental unit. Sow body weights (BW) and ultrasound backfat thickness were recorded at the time of entry to and exit from the lactation facilities. Litter weights were recorded at birth and at weaning, and litter inventories were recorded at birth, upon completion of cross-fostering within treatment, on d 5 of lactation, and at weaning. Ultimately, 185 sows completed the study and were included in statistical analyses. Data were analyzed using the lme4 package of R 4.1.2, and the statistical models included the fixed effect of treatment and the random effects of parity and farrowing batch. Data were assumed to be normally distributed, except for pig survival and conception rate, which were analyzed using generalized linear mixed models specifying a binomial distribution. Total and live litter size did not differ at birth, nor did litter birth weight (Table 1). Inclusion of the phytogenic feed additive increased (P &amp;lt; 0.05; ADFI in lactation from 6.21 to 6.74 kg per day. In turn, the phytogenic feed additive tended (P &amp;lt; 0.10) to increase litter wean weight, which was driven by numerically increased piglet survival (87.47% for control and 89.37% for phytogenic, P = 0.126) and a tendency (P &amp;lt; 0.10) for increased average pig weaning weight. Sow BW did not differ at entry to lactation, though sow weight at weaning tended to be greater (P &amp;lt; 0.10) when the phytogenic feed additive was fed. The phytogenic feed additive also increased (P &amp;lt; 0.05) subsequent conception rate from 88.7 to 97.8%. In conclusion, feeding a phytogenic feed additive during summer conditions increased lactation feed intake, which resulted in increased weaning weight and improved subsequent conception rate.

Enhancing Mechanical Properties of Corn Bran Arabinoxylan Films for Sustainable Food Packaging.

Arabinoxylan (AX)-based films can improve the mechanical characteristics of biodegradable materials when utilized for food packaging. However, the mechanical properties of AX films for food packaging applications require thorough investigation to establish their viability. In this study, AX was extracted from corn bran coproducts of dry-milling (DCB), wet-milling (WCB), and dried distiller's grains with solubles (DDGS) using an acid-alkali method. Packaging materials were produced using these AX extracts, each combined with laccase and sorbitol, forming the basis for three different films. These films were then modified by immersing the surface in a lipase-acetate solution. We evaluated their mechanical characteristics, including thickness, tensile properties, tear resistance, and puncture resistance. The thickness and tensile properties of the modified AX films derived from DCB and DDGS showed significant improvements (p < 0.05) compared to the unmodified AX films. In contrast, the modified AX films from WCB showed no significant changes (p > 0.05) in thickness and tensile properties compared to the unmodified WCB AX films. A significant increase in tear resistance (p < 0.05) was observed in all modified AX films after immersion in the lipase-acetate mixture. While puncture resistance was enhanced in the modified AX films, the improvement was not statistically significant (p > 0.05) compared to the unmodified films. The presence of hydroxyl (OH) and carbonyl (CO) groups on the surfaces of AX films from DCB and DDGS, modified by the lipase-acetate solution, suggests excellent biodegradability properties. The modification process positively affected the AX films, rendering them more bendable, flexible, and resistant to deformation when stretched, compared to the unmodified AX films.

Hydroponic barley supplementation fed with high-protein diets improves the production performance of lactating dairy cows

The study investigated the effects of dietary protein level and the inclusion of hydroponic barley sprouts (HB) on lactation performance, blood biochemistry and N use efficiency in mid-lactation dairy cows. Treatments were arranged in a 2 × 2 factorial design with 2 crude protein (CP) levels [16.8% and 15.5% of dry matter (DM)], with HB (4.8% of DM, replacing 4.3% of alfalfa hay and 0.5% of distillers dried grains with solubles (DDGS)) or without HB. Forty-eight multiparous Holstein dairy cows (146 ± 15 d in milk, 40 ± 5 kg/d of milk) were randomly allocated to 1 of 4 diets: high protein diet (16.8% CP, HP), HP with HB (HP+HB), low protein diet (15.5% CP, LP), or LP with HB (LP+HB). An interaction between CP × HB on dry matter intake (DMI) was detected, with DMI being unaffected by HB inclusion in cows fed the high CP diets, but was lower in cows fed HB when the low CP diet was fed. A CP × HB interaction was also observed on milk and milk protein yield, which was higher in cows fed HB with HP, but not LP. Inclusion of HB also tended to reduce milk fat content, and feeding HP resulted in a higher milk protein and milk urea N content, but lower milk lactose content. Feed efficiency was increased by feeding HP or HB diets, whereas N efficiency was higher for cows fed LP or HB diets. There was an interaction on the apparent total-tract digestibility of DM and CP, which was higher when HB was fed along with HP, but reduced when fed with LP, whereas the digestibility of ADF was increased by feeding low protein diets. In conclusion, feeding a low protein diet had no adverse effect on cow performance, while feeding HB improved milk and milk component yield, and N efficiency when fed with a high CP diet, but compromised cow performance with a low CP diet.