Types Of Disinfectants Research Articles

Molecular studies on Clostridium perfringens from broiler chicken farms in Egypt with special reference to biofilms and resistance to disinfectants

At the most cases, C. perfringens has the ability to form biofilms in order to persist in the environment as, the predominant structural state of bacteria in nature is biofilms which increase the bacterial tolerance to disinfection that can lead to economic and health concerns. Two hundred eighteen samples (88 liver samples and 130 intestinal samples) were collected from clinically affected chickens with necrotic enteritis and revealed the presence of C. perfringens with percentage of 94.6 % and 78.4% respectively with total percentage 86.5 %. Twenty suspected C. perfringens isolates were examined by PCR and the results revealed that all of the examined samples were identified as C. perfringens and all samples revealed bands of AppB gene at 109 bp, 17 of 20 samples gave iolD gene bands at 98 bp and 13 of 20 samples gave ripH gene bands at 105 bp. These isolates were used for antiobiotic susceptibility testing and results showed that all isolates were highly susceptible to imipenem. Also, Most of isolates were highly susceptible to Ampicillin and Amoxicillin but, all isolates were resistant to Sulphamethoxazole trimethoprim, Colistin, Tylosine and Lincomycin and also, all isolates showed multidrug resistance. And these isolates were screened for biofilm producing ability and results showed that all the twenty isolates were strong biofilm producers except three isolates which were moderate biofilm producers, Nine types of disinfectants commonly used on poultry farms were used to test their efficacy on C. perfringens biofilms and Sodium hypochlorite 0.027% was the most efficient disinfectant on C. perfringens biofilms.

Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes the global COVID-19 pandemic. Limited studies have been performed on various types of disinfectants utilized to control the spread of this highly contagious virus. This study aimed to investigate the inactivation of SARS-CoV-2 surrogate virus, hCoV-229E using an <i>in vitro</i> to test the anti-infectivity activity of the humidifier buffers (A and B, Lumichem<SUP>TM</SUP>). A real-time reverse transcriptase quantitative PCR (RT-qPCR) assay was used to evaluate the effectiveness of these disinfectants on the degradation of viral RNA in a time dependent manner. The effects of disinfectants on viral infectivity were determined using a tissue culture infectious dose (TCID50) assay of a surrogate virus, hCoV-229E, in MRC-5 cell culture. The results demonstrated that the Lumichem<SUP>TM</SUP> buffers A and B had a 2 to 3-log₁₀ reduction inactivation using cell culture after a short exposure compared to the control, indicating the disinfection efficacy of the tested anti-infectivity compounds. The Lumichem<SUP>TM</SUP> buffers A and B in addition did not affect the viral genomic RNA of a surrogate virus, hCoV-229E, thus representing an additional benefit with a negligible impact to operators and those in close contact when providing in-situ operational cleaning.

Can Medical-Grade Gloves Provide Protection after Repeated Disinfection?

The advent of the COVID-19 pandemic has generated an increased consumption of personal protective equipment (PPE), including gloves and masks, by healthcare workers and by the general public at a global scale. This has generated substantial shortage of these single-use and disposable PPEs that will end up as a landfill waste. Extending the life cycle of PPEs, such as gloves, by disinfecting treatments could help mitigate these concerns. However, the effect of various disinfection treatments on the functionality of gloves is unknown. In this study, six commonly used viral disinfection treatment methods (i.e., ultraviolet (UV) radiation, dry heat, steam, alcohol, chlorine compounds, and quaternary ammonium compounds) were evaluated for their effect on the performance attributes of two commonly used medical-grade gloves and nitrile and vinyl (latex) gloves. The barrier properties of both gloves against water and ethanol vapor flux were not affected up to 10 cycles of disinfection cycles. However, the increase in the disinfection cycle from 10 to 20 slightly reduced their barrier properties with minor variation in the type of disinfection and glove type. Infrared spectroscopy and microscopy investigations confirmed that both types of gloves could withstand up to 20 cycles of disinfection treatments with no observable change in the chemical structure and surface morphology of the disinfected surfaces, respectively. Lastly, tear property testing of the gloves indicated little to no change from the baseline after 20 cycles of treatment in both the nitrile and vinyl-based gloves. Overall, this study indicated that alcohol, UV, and heat treatment could be acceptable disinfection methods that allow the reuse of gloves up to 20 cycles. Such repeated disinfection of gloves not only reduces the strain on the supply of gloves but also decreases the postconsumer landfilled waste and environmental footprint of gloves.

Basic Principles of Disinfection and Sterilization in Intensive Care and Anesthesia and Their Applications during COVID-19 Pandemic.

Understanding the concepts of disinfection, sterilization, cleaning and asepsis is of prime importance to prevent transmission of infection to patients and to protect healthcare workers (HCWs). Proper disinfection of surfaces after cleaning, an important consideration at all times, has assumed special significance during the current pandemic. The global shortage of disposable equipment such as personal protective equipment (PPE), specifically N95 masks and surgical 3 ply masks, and other items makes the HCWs vulnerable to transmission of infection while caring for these patients. Therefore, resterilization of such items has assumed equal importance. Cleaning, the first step in the process of sterilization, is of vital importance to reduce bioburden. The type of disinfection required depends on the nature of the equipment and its intended use. For example, critical items need high-level decontamination. In this narrative review, we elaborate on the methods of decontamination and sterilization. Many chemicals can be used for both sterilization and disinfection, and the difference lies in the concentration of the chemical and exposure time. We have also summarized strategies which can be used for resterilization of single-use items, in view of the shortages caused by the current pandemic.How to cite this articleKothekar AT, Kulkarni AP. Basic Principles of Disinfection and Sterilization in Intensive Care and Anesthesia and Their Applications during COVID-19 Pandemic. Indian J Crit Care Med 2020;24(11):1114–1124.

Chlorine and peracetic acid in decentralized wastewater treatment: Disinfection, oxidation and odor control

Decentralized wastewater treatment is a viable and sustainable alternative to having universal access to sanitation, especially for developing countries and small communities. Chlorine and peracetic acid (PAA) have been studied widely to promote wastewater disinfection, however there are few comparative studies applied to decentralized wastewater treatments. This paper investigated the application of chlorine and PAA in two biological reactor effluents regarding disinfection, organic matter oxidation, and odor control. Full factorial design was used to quantify the effect of the Chlorine and PAA dose (5, 10, and 15 mg L−1) and contact time (5, 10, and 15 min) in the batch experiments. Chlorine and PAA were inefficient in reducing the organic matter showing an increment in the chemical oxygen demand (COD) concentration in most of the tests. Sulfide was oxidized by chlorine (0.0–39.9 %) and PAA (3.5–73.5 %) for both reactor effluents. Inactivation results from E. coli and total coliform by chlorine and PAA showed that the effluent quality has a stronger influence than the disinfectant type. The best operational condition (15 mg L−1, 15 min) were also tested in the continuous mode flow, whose results are in agreement with those found in the batch tests. These results provide valuable support in terms of establishing practical guidelines for chlorine and PAA applications in decentralized wastewater treatment in Brazil.

Sensitivity of Vibrio parahaemolyticus, V. vulnificus and V. harveyi Against Chloroxylenol (4-Chloro-3,5-dimethylphenol , C8H9ClO) Antiseptic and Pine Oil Disinfectant

Vibrio spp. genus is known as a marine indigeneous bacteria. Vibrio parahaemolyticus, V. vulnificus and V. harveyi are pathogenic Vibrio. This study aims to assess the sensitivity of three Vibrio species (V parahaemolyticus, V. vulnificus and V. harveyi) isolated from shrimp pond against two type of disinfectant with different active compound namely Chloroxylenol (4-Chloro-3,5-dimethylphenol, C8H9ClO) and pine oil. The assessment was done by Kirby-Bauer disk diffusion methods in Zobell agar media with two different concentration (10 and 100 ppm) and replicated in three times. Sensitivity of Vibrio spp. was analized based on the inhibition zone activity produced by disinfectant. Results showed that sensitivity of Vibrio spp. against disinfectant Chloroxylenol 4.8% at 100 ppm were higher than 10 ppm. The increment of V parahaemolyticus was 182 %, V. vulnificus was 47 % and V. harveyi was 43 %, respectively. Susceptibility of antiseptic with Chloroxylenol 4.8% at 100 ppm was arised to 152 % (V. parahaemolyticus), 43 % (V. vulnificus) and 31 % (V. harveyi) when compared to 2.5% pine oil disinfectant. It can be concluded that Chloroxylenol 4,8 % active compound and pine oil were able to inhibit the Vibrio spp. growth.

Evaluation of a Mobile Two-Stage Decontamination System using a Power Washer Combined with Eight Disinfectant Treatments

With the increased frequency of pandemics that threaten the spread of zoonotic diseases associated with agricultural commodities and trade, it is becoming a national priority to advance more effective and efficient decontamination technologies. A field study was conducted to evaluate a two-stage, mobile power washing system. The study factors were power washing, disinfectant type, sample surfaces, and number of repeat disinfectant applications. Study factors were evaluated based on log10 reduction of viable Bacillus subtilis spores on inoculated sample surfaces. Diluted bleach from Clorox Concentrate, applied without power washing, had the greatest sporicidal activity when applied three times to non-porous surfaces (steel washers), which resulted in a 3.0 log10 reduction of viable spores. The two-stage decontamination treatment with the greatest sporicidal activity was power washing porous surfaces (wool fabric), followed by three applications of EasyDECON DF-200, resulting in a 4.8 log10 reduction of viable spores. The results showed that power washing was the most important factor for dislodging spores and overall decontamination effectiveness. Also, sporicidal activity was slightly greater for non-porous surfaces compared to porous surfaces. Repeated applications of disinfectants resulted in little to no improvement in sporicidal efficacy. The results from this field study were comparable with a similar two-stage equipment decontamination study, which was conducted the year before this study. Further research is needed to evaluate large stationary decontamination systems and refine any interactions between power washing parameters and innovative sanitation methods.

Study of disinfectant type effect on the organohalogen compounds formation in water treatment

In practice of water supply, mainly, chlorine gas is used in the preparation of drinking water supply system, which is toxic, combustible and explosive. In this regard, the urgent problem is the replacement of gaseous chlorine with a safer disinfectant, which provides at least equivalent results at all stages of water treatment. We have studied the possibility and effectiveness of using technical sodium hypochlorite. Contents of organic substances in river water and the concentration of organohalogen compounds in drinking water, regardless of the season (25% on average) with the use of sodium hypochlorite as a disinfectant were established in this research work. It was shown that periodically during 6 months of the year (March-August) the concentration of chloroform and trichlorethylene exceeded MPC normalized values of these substances in drinking water, which necessitated the development of a technology for water purification from organohalogen compounds.

Suckling piglet coccidiosis on farms in the Czech Republic - A pilot study

Suckling piglet coccidiosis is a common diarrhoeal disease of unweaned pigs caused by Cystoisospora suis with previously reported herd prevalences of 60% and more. The parasite quickly spreads in affected herds and causes malabsorption and a reduced and uneven body weight gain. The only drug currently licenced for the control of porcine cystoisosporiasis is toltrazuril, which suppresses parasite development and the corresponding diarrhoea. A recent study detected high prevalences of C. suis on swine farms in Austria, Germany, Spain and the Czech Republic independent of the treatment. Here, the situation in the Czech Republic is evaluated in more detail. In total, faecal samples from 161 litters (two samples/litter in the second and third week of life) from 17 farms (250–2 000 sows) were examined for the presence of C. suis oocysts by autofluorescence and for faecal consistency. An accompanying questionnaire revealed details on the herd management and treatments. Eight farms applied toltrazuril on the 3&lt;sup&gt;rd&lt;/sup&gt;, 4&lt;sup&gt;th&lt;/sup&gt; or 5&lt;sup&gt;th&lt;/sup&gt; day of life. Overall, 70.6% of the farms and 32.2% of the litters were positive for C. suis at least once, 39.4% in the untreated litters (n = 76) and 24.8% in the treated litters (n = 85). Diarrhoea occurred on eight farms (six with the toltrazuril treatment) in 11.9% of the untreated and 2.5% of the treated litters. This study shows that coccidiosis is still common in Czech swine farms and that treatment in the recommended time frame may reduce the diarrhoea, but cannot interrupt the parasite’s life cycle. Some type of effective disinfection, which may have contributed to a more effective parasite control, was not in place in any of the farms.

Disinfection of 3D-printed protective face shield during COVID-19 pandemic

This study assessed the disinfection using 70% ethanol; H2O2-quaternary ammonium salt mixture; 0.1% sodium hypochlorite and autoclaving of four 3D-printed face shields with different designs, visor materials; and visor thickness (0.5-0.75 mm). We also investigated their clinical suitability by applying a questionnaire to health workers (HW) who used them.Each type of disinfection was done 40 times on each type of mask without physical damage. In contrast, autoclaving led to appreciable damage.

DEZENFEKSİYON ÜNİTESİ RİSK ANALİZİ: İÇME SUYU ARITMA TESİSİ

In this study, risk analysis and risk assessment study of risk factors arising as a natural result of hazards and hazards that may occur in disinfection units of drinking water treatment plants were carried out. Different processes are applied in drinking water treatment plants according to the water bodies used as sources. The most widely used disinfectants in our country and worldwide are chlorine, chloramine and ozone. There are risk analysis studies carried out in wastewater treatment plants in our country, but there is no study carried out in drinking water treatment plants, especially disinfection units. In this study, the risks arising from the working environment, disinfectant type and employees in the drinking water treatment plant disinfection units were determined. All risks were interpreted according to the 5x5 matrix risk analysis method. It has been determined that the main problem in disinfection units is the inadequate training of people, the use of appropriate personal protective equipment and the disinfectant exposure. It is inevitable that disinfection units will be a very reliable place in terms of both worker and environmental health with trainings to be given at regular intervals.

Manganese release from corrosion products of cast iron pipes in drinking water distribution systems: Effect of water temperature, pH, alkalinity, SO42− concentration and disinfectants

Manganese accumulated in corrosion scales on drinking water distribution systems (DWDSs) can be released into bulk water, causing discolouration and thereby leading to customer concerns about drinking water quality. A static release experiment was conducted on iron pipe scales under three different temperatures, pH values, alkalinity values, sulfate (SO42−) concentrations, and disinfectants to study the separate effect of these factors on Mn release from pipe scales under stagnant conditions. Results showed that more Mn was released from corrosion scales under the conditions of lower pH, lower alkalinity, higher temperature, and higher SO42− concentrations. Three commonly used disinfectants, sodium hypochlorite (NaClO), chlorine dioxide (ClO2), and monochloramine (NH2Cl) were found to inhibit the release of Mn from iron corrosion scales, with the ranked order of inhibitory effect of ClO2≈NaClO > NH2Cl under the same CT (product of disinfectant concentration and contact time) value. The orthogonal experimental results indicated that SO42− and alkalinity had extremely significant effects on the release of Mn from pipe scales, while pH and disinfectant type had a significant impact on the release of Mn from pipe scales. Thus, the SO42− concentration and alkalinity of the bulk water should be determined to avoid excessive release of Mn into drinking water. However, further investigation of the effect of disinfectants on Mn release in DWDSs is necessary. This research helps establish a systematic understanding of the influential factors in Mn release from pipe scales into bulk water, as well as their significant relationships.

Ozonation for feed raw materials disinfection (review)

The issues of disinfection of raw materials for feed production by the method of ozonation were considered in the review. Ozonation could be a universal method of plant raw materials disinfection in agricultural enterprises and formula-feed plants. A research objective is to generalize and analyze scientific publications devoted to the feed raw materials disinfection by ozone, as well as to clarify information on rational parameters of such type of disinfection and its effect on the quality of feed. Moreover, we assessed the possibility of further use of ozonation in the production process. For the research of the subject, we searched and selected scientific articles published from 2005 to 2020 and conducted a systematic review. Ozonation was found to be an effective method for feed decontamination that destroys pathogenic bacteria and fungi. This method is also used to detoxify feed and control pest insects. Ozonation is not yet widely used in agricultural production and formula-feed industry to ensure the biological safety of feed raw materials. Additional research aimed at improving the efficiency of ozonation by standardization of parameters for its implementation to ensure the broadest possible application of the method under study should be carried out. The development of technical solutions that increase the diffusion of gaseous ozone will significantly increase the safety and preservation of raw materials. Rational values of ozone concentration, processing time, etc., for each type of raw materials, as well as for mycotoxins, pesticides, microorganisms and insects, including different stages of their growth and development, should be defined. Special attention should be also focused on the study of the complex efficiency of ozonation concerning several pathogenic factors.

Incidence of infection in non-tunnelled thoracic epidural catheters after major abdominal surgery.

Thoracic epidural analgesia is beneficial after major abdominal surgery, though side-effects and complications are rare but potentially devastating. The incidence of catheter-related infection is approximately 5.5%. Several guidelines have been recommended to prevent complications during thoracic epidural catheterization. Tunnelling is often recommended to reduce the incidence of infections and dislocations. A retrospective, single-centre analysis of our acute pain service database was performed between 2010 and 2018. The hygiene measures of the German Society of Anaesthesiology have been incorporated in our standard operating protocol since 2009. The procedure remained constant, but the skin disinfectant was changed from propan-2-ol to propan-2-ol with octenidine in 2014. Tunnelling of catheters was not performed. We analysed the incidence of catheter-related infections (primary endpoint) and effect of the used disinfectant (secondary endpoint). A total of 2755 patients underwent elective major abdominal surgery with thoracic epidural catheterization. Sixteen patients (0.6%) showed symptoms of mild catheter-related infection. Moderate or severe infections were not observed in any patient. The type of disinfectant did not show any significant effect on the incidence of infection. The incidence of catheter-related infections was low, and only mild signs of infection were observed. Non-tunnelling could be an alternative to tunnelling, especially if hygiene protocols are followed, and the duration of catheter use is short. A comprehensive database and regular examinations by trained staff are essential for early detection of abnormalities and immediate removal of the catheter, if required.

The sug operon involves in resistance to quaternary ammonium compounds in Listeria monocytogenes EGD-e.

In recent years, an increasing number of Listeria monocytogenes strains with resistance to quaternary ammonium compounds (QACs) have been reported. However, the genetic basis for QACs resistance in L. monocytogenes remains poorly understood. In the present study, we have characterized the operon lmo0852/lmo0853/lmo0854 (designated sugR/sugE1/sugE2) that contributes to QACs' resistance in L. monocytogenes EGD-e. We constructed the gene deletion mutants and the complemented strains, determined minimum inhibitory concentrations (MICs) of these strains against antimicrobial agents, assessed the transcription levels of target genes by RT-qPCR, and measured the promoter activity by using β-galactosidase assays. We also investigated the interaction between the promoter DNA and the putative regulatory protein by electrophoretic mobility shift assay (EMSA). The sug operon consists of a putative TetR family regulator encoded by sugR and two small multidrug resistance (SMR) efflux pumps encoded by sugE1 and sugE2. Our results showed that either SugE1 or SugE2 is sufficient for QACs' resistance, indicating their function overlapping in QACs' resistance. Interestingly, lacking one sugE gene could lead to a significant increase in transcription of the other sugE gene in the presence of benzalkonium chloride (BC). Additionally, SugR negatively regulates the transcription of the sug locus by binding to the operon promoter. Given that QACs are commonly used in food industry, the findings from this study will help us to have a better understanding of the adaptations of L. monocytogenes to this type of disinfectant. Key points •Either SugE1 or SugE2 was sufficient for QACs resistance. •The functions of two sug genes overlap and compensate each other in QACs resistance. •SugR is the transcriptional suppressor of sugE1 and sugE2. •SugR regulates the sug locus by binding to the operon promoter.

The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles.

BackgroundMesoporous calcium-silicate nanoparticles (MCSNs) have good prospects in the medical field due to their great physicochemical characteristics, antibacterial activity and drug delivery capacity. This study was to analyze the antibiofilm activity and mechanisms of silver (Ag) and zinc (Zn) incorporated MCSNs (Ag/Zn-MCSNs) with different percentages of Ag and Zn.MethodsAg/Zn(1:9, molar ratio)-MCSNs and Ag/Zn(9:1, molar ratio)-MCSNs were prepared and characterized. Endocytosis of nanoparticles by Enterococcus faecalis (E. faecalis) treated with Ag/Zn-MCSNs was observed using TEM to explore the antibacterial mechanisms. The antibiofilm activity of Ag/Zn-MCSNs with different ratios of Ag and Zn was tested by E. faecalis biofilm model in human roots. The human roots pretreated by different Ag/Zn-MCSNs were cultured with E. faecalis. Then, SEM and CLSM were used to observe the survival of E. faecalis on the root canal wall. Cytotoxicity of the nanoparticles was tested by CCK8 kits.ResultsThe Ag/Zn-MCSNs release Ag+ and destroy the cell membranes to kill bacteria. The MCSNs containing Ag showed antibacterial activity against E. faecalis biofilms in different degrees, and they can adhere to dentin surfaces to get a continuous antibacterial effect. However, MTA, MCSNs and Zn-MCSNs could not disrupt the bacterial biofilms obviously. MCSNs, Ag/Zn(1:1, molar ratio)-MCSNs and Ag/Zn(1:9)-MCSNs showed no obvious cytotoxicity, while Ag-MCSNs and Ag/Zn(9:1)-MCSNs showed cytotoxicity. Zn-MCSNs can slightly promote cell proliferation.ConclusionAg/Zn-MCSNs have good antibiofilm activity. They might achieve an appropriate balance between the antibacterial activity and cytotoxicity by adjusting the ratio of Ag and Zn. Ag/Zn-MCSNs are expected to be a new type of root canal disinfectant or sealer for root canal treatment.

Conhecimento de docentes e discentes de um curso de Odontologia sobre os aspectos legais que envolvem a utilização de dentes humanos extraídos

O estudo verificou o nível de conhecimento do corpo docente e discente de um curso de Odontologia acerca dos aspectos éticos e legais envolvidos na obtenção e manipulação de elementos dentários humanos extraídos. Foi aplicado um questionário semiestruturado aos docentes e discentes a partir do 2º período, por se constituírem os usuários de dentes humanos nas atividades de ensino pré-clínico e de pesquisa. Os dados foram analisados utilizando-se a estatística descritiva para as perguntas fechadas, além do Discurso do Sujeito Coletivo para as perguntas abertas. Participaram 51 docentes de um total de 55 e 201 discentes do total de 288, dos quais 72,55% e 72,60%, respectivamente, reconheceram o elemento dentário como um órgão. Quanto à forma de obtenção, 39,49% dos discentes relataram obter os dentes em consultórios odontológicos e 33,76% em unidades de saúde, sendo que 91,04% destes e 80,39% dos docentes desconhecem qualquer tipo de comercialização. Em relação à biossegurança, tanto os discentes (78,61%) quanto os docentes (90,20%) reconhecem o risco biológico na manipulação desses elementos e realizam algum tipo de desinfecção. Considerando-se os aspectos legais, 68,63% dos docentes e 20,10% dos discentes afirmaram ter conhecimento da existência de uma regulamentação, o que não se refletiu de forma explícita nas respostas obtidas. Do material textual gerado pelas perguntas abertas emergiram três categorias (forma de obtenção dos dentes utilizados, comercialização de dentes e risco biológico), interpretadas à luz de discursos agregados dos segmentos (docentes e discentes) entrevistados. Pode-se concluir que tanto docentes quanto discentes apresentaram conhecimento incipiente sobre os aspectos legais que envolvem a obtenção e manipulação de elementos dentários humanos extraídos.

Effect of Sanitizers and Disinfectants in Staphylococcus Saprophyticus

Background/Objectives: In the dental environment, handpiece or ultrasonic scaler used in treatment causes aerosols and various pathogenic microorganisms. Many types of pathogens cause infectious diseases. This study is to identify surface disinfectants suitable for the management of Staphylococcus saprophyticus bacterial species, one of the causes of infection in medical institutions to use them as standardized surface management resources for infection control in dental clinic.Method/Statistical Analysis: Commonly used 7 types of disinfectants were classified to select commercialized disinfectants. Samples were taken by rubbing the surface of the unit chair with a sterile swab. In order to check the bacterial species cultured in the medium, a single colony was purely separated by streaking. To identify the bacterial species, DNA was extracted from the bacteria and PCR was performed. the mean and standard deviation of each group. One-way ANOVA and Scheffe’s post-test were carried out to identify the clear zone of paper discs and significant differences between groups.Findings: The test for bacterial identification was carried out for the surface flora of the unit chair. As a result, S. saprophyticus was identified on the surface. According to the result of comparative analysis of the clear zone size of each disinfectant, sodium hypochlorite (NaOCl), a chlorine disinfectant was found to have the highest disinfection effect (8.52mm), followed by Distell, an ammonium compound disinfectant (2.72).Improvements/Applications: The result of the analysis of the clear zone of the disinfectant showed excellent antibacterial activity in 0.3% NaOCl and 0.5% Distel, indicating that they are disinfectants suitable for S. saprophyticus used in the study.

Analisis faktor risiko terjadinya phlebitis di RSUD Puri Husada Tembilahan

Phlebitis is one of the standard indicators of hospital's minimum service with the dimension of patient safety quality. Risk factors for phlebitis include disease, intravenous catheter size, distance insertion place with joints, type of fluid, technique disinfection, dressing techniques and long infusion attached. The research aims to analyse the risk factors of phlebitis. The methods used were observational crossectional analytic conducted in Puri Husada Tembilahan HOSPITAL against 97 patients. The results showed that the factors related to the occurrence of phlebitis is the distance of insertion place with the joints, types of liquids, techniques of disinfection, technique dressings, and prolonged infusion is attached, while the disease factors and the size of the catheter intravenously unrelated to the occurrence of phlebitis. The most dominant factor is related to Phlebitis is disinfection technique (OR = 4.567). The results of this research can be information for nurses in the prevention of phlebitis by conducting disinfection and dressing with sterile technique during the installation of infusion, monitor drip infusion according to therapy, insertion with Distance &gt; 3.5 cm from the joints, and replacing the place of insertion in the patient's attached infusion &gt; 72 hours.

Identification, formation and control of polar brominated disinfection byproducts during cooking with edible salt, organic matter and simulated tap water

Edible salt is essential to the health of humans and serves as a seasoning universally. Besides chloride, edible salt also contains other anions such as bromide, fluoride, sulfate, and carbonate due to incomplete removal during raw salt refinement. In a household cooking (e.g., soup making) process, a chlorine/monochloramine residual in tap water could react with bromide in edible salt and organic matter in food (e.g., rice, wheat) to form numerous brominated disinfection byproducts (Br-DBPs) at significant levels, which might induce adverse health effects to human beings. In this study, we solicited 20 edible salts of different types (i.e., sea salts, well and rock salts, lake salts, and bamboo salts) from nine countries and determined their bromide levels to be 67–375 mg/kg, with an average level of 173 mg/kg. A total of 25 polar Br-DBPs were detected and identified with structures/formulae in cooking water samples using ultra performance liquid chromatography/electrospray ionization-triple quadruple mass spectrometry (UPLC/ESI-tqMS) and high-resolution mass spectrometry. Effects of cooking conditions (e.g., disinfectant type and level, edible salt dose, organic matter type and dose, sequence and time interval of adding organic matter and salt, etc.) on the formation of polar Br-DBPs were investigated, and optimized cooking conditions with minimized formation of polar Br-DBPs were determined. Further aided with an Hep G2 cell cytotoxicity assay, it was found that the overall cytotoxicity of chlorinated and chloraminated cooking water samples prepared after cooking condition optimization was reduced by 57% and 22%, respectively, compared with those prepared before cooking condition optimization.