Diseases In Farm Animals Research Articles

Infectious and Non-Infectious Diseases of Farm Animals in Natore District of Bangladesh

Farm animals in Bangladesh play a crucial role in providing nutrition and are a vital component of the rural economy. This study aimed to evaluate the frequency of prevalent infectious and non-infectious diseases among farm animals in the Natore district of Bangladesh. Six Upazila Veterinary Hospitals in the Natore district of Bangladesh participated in the research from January 2023 to December 2023. We identified 76,040 clinical cases by considering the owner's statement, conducting a general examination, observing clinical indicators, and performing laboratory diagnostics. The incidence of endoparasitic infection was the highest (47.73%), while bacterial disease infection was less frequent (1.94%). Among farm animals (cattle, buffalo, goats, sheep, and pigs) goats had the highest prevalence (54.55%) while buffaloes (3.592%) and pigs (0.068%) had the lowest prevalence. The prevalence of both infectious and non-infectious diseases was highest during the rainy season (44.37%), followed by the winter (30.48%) and summer season (25.14%). Although pig farming is uncommon in Bangladesh, we have observed the raising of pigs in the Natore district. Regrettably, parasitic infestation, specifically lice and roundworms, affects a significant number of these piglets. The frequency of lice and roundworm infestation in pigs is 0.02%. These findings suggest a significant prevalence of both infectious and non-infectious disorders. Our research reveals significant insights for prioritizing research on specific diseases and implementing efficient control strategies against them. Therefore, it is imperative for veterinarians and development partners to act to prevent and mitigate the impact of both infectious and non-infectious diseases in farm animals in Bangladesh. Res. Agric. Livest. Fish. Vol. 11, No. 3, December 2024: 327-341

Repeatability and predictability of lying and feeding behaviours in dairy cattle

Subtle changes in behaviour, which may be useful as early indicators of disease or stress in farm animals, can be detected using precision livestock technologies. However, as animals often display differences in their baseline behaviours from one another, individual variation needs to be measured and accounted for if we want to successfully detect abnormalities. In addition to consistent individual differences in their mean behaviour, which are called behavioural types and measured by repeatability, animals may differ in the amount of day-to-day variation around their own mean. This is known as predictability and it is very rarely measured in animals as it requires data with high granularity for extended periods of time, which is hard to obtain without precision technologies. Here we aim to quantify the repeatability and predictability of lying and feeding, two behaviours which have been investigated as potential indicators of disease with inconsistent results, in dairy cows. We used data on daily lying behaviours from leg-mounted sensors for 1439 cows and data on daily feeding behaviours from neck-mounted sensors for 2584 cows belonging to multiple herds to quantify the variation between individuals, the individual differences in predictability and any correlations between individual behaviour type and predictability of the same behaviour. Using multivariate double hierarchical generalised linear models, we were able to quantify individual variation in feeding and lying behaviours of adult cows for the first time. Consistent variation between individuals was present for all measures of lying and feeding with repeatability values of 0.38 for daily feeding time and 0.34 for daily lying time. Individuals also varied in their levels of predictability with coefficients of 0.46 and 0.29 for the daily lying and feeding time respectively. Lastly, there were significant positive correlations between several behaviours and the amount of residual intra-individual variation for the same behaviour (mean lying bout length: 0.93, mean feeding bout length: 0.88), indicating that cows with the longest bouts of the two behaviours were also more variable and unpredictable, perhaps due to an opportunistic strategy. These results highlight the importance of measuring individual variation in behaviour, supporting the evidence of personality traits in cattle, as well as differences in predictability, which must be taken into account in any use of behavioural indicators for early disease diagnosis.

Making a mark on the farm: the marks and traces of farm animals and infectious diseases in northern England

ABSTRACT Farmed animals are expected to move through farmed spaces in certain ways to maximise their productivity. These spaces are also designed to limit the movement of disease-causing organisms. However, both types of lifeforms do not always move in expected ways. We focus on the mark-making of these organisms to explore: 1) the evidence of their movements through farm spaces; and 2) the effects of these movements on managing farm animal disease. We explore these questions via social-scientific and artistic practices. The social science draws on in-depth interviews with UK cattle and sheep farmers, and farm advisors. The artistic component draws on work conducted by an ‘artist in residence' engaging with farm animals and farmer-livestock relationships. Farm animals and infectious micro-organisms were found to move in different ways and create different marks and traces across farms, bodies, and how diseases were managed. These lifeforms often frustrated biosecurity practices of exclusion and enclosure and existed on a spectrum of disease risk. Human actors needed to learn to become attuned to lifeform movements in order to enact disease management. We conclude by suggesting a continued focus in future social-scientific research on how the ‘sub-animal body' contributes to the enacting of farm disease management.

Molecular and serological prevalence rates of Neospora caninum infection in dogs from Jordan.

Neosporosis is a proven disease of farm animals and dogs caused by Neospora caninum. This cross-sectional study investigates N. caninum prevalence and seroprevalence among 268 dogs. Nc5 gene PCR was carried out on dog faeces and confirmed by sequencing. Seroprevalence was detected using an indirect fluorescent antibody test (IFAT). Three age groups, gender, locality (Amman, Irbid, and Zarqa Governorates), dog type (stray, pet, and breeding), place of living (indoor/outdoor), food type (raw/cooked), having diarrhoea, having abortion in the area, and having animals nearby were tested as independent variables for associations with positivity to N. caninum using univariate and multivariable logistic regression analyses. The true prevalence of N. caninum was 34.3% (95% CI 28.4, 40.5) using the Nc5-PCR test. The true seroprevalence rate of N. caninum among dogs in Jordan was 47.9% (95% CI 41.4, 54.5) using IFAT. The sequenced isolates of Nc5-PCR products (n = 85) matched three N. caninum strains, namely, NcHareGre (n = 70, 82.4%, 95% CI 72.6-89), NC MS2 (n = 14, 16.5%, 95% CI 9.3-26.1), and L218 (n = 1, 1.2%, 95% CI 0.03-6.4). The three strains were isolated previously from three different countries and continents. N. caninum shedding is associated with abortion among dogs and animals in the area (odds ratio = 3.6). In Amman and Zarqa, living indoors reduced seroprevalence at 0.45, 0.24, and 0.02 odds ratios, respectively. Jordan shares three molecular N. caninum strains with three different countries and continents.

MicroRNAs: exploring their role in farm animal disease and mycotoxin challenges.

MicroRNAs (miRNAs) serve as key regulators in gene expression and play a crucial role in immune responses, holding a significant promise for diagnosing and managing diseases in farm animals. This review article summarizes current research on the role of miRNAs in various farm animal diseases and mycotoxicosis, highlighting their potential as biomarkers and using them for mitigation strategies. Through an extensive literature review, we focused on the impact of miRNAs in the pathogenesis of several farm animal diseases, including viral and bacterial infections and mycotoxicosis. They regulate gene expression by inducing mRNA deadenylation, decay, or translational inhibition, significantly impacting cellular processes and protein synthesis. The research revealed specific miRNAs associated with the diseases; for instance, gga-miR-M4 is crucial in Marek's disease, and gga-miR-375 tumor-suppressing function in Avian Leukosis. In swine disease such as Porcine Respiratory and Reproductive Syndrome (PRRS) and swine influenza, miRNAs like miR-155 and miR-21-3p emerged as key regulatory factors. Additionally, our review highlighted the interaction between miRNAs and mycotoxins, suggesting miRNAs can be used as a biomarker for mycotoxin exposure. For example, alterations in miRNA expression, such as the dysregulation observed in response to Aflatoxin B1 (AFB1) in chickens, may indicate potential mechanisms for toxin-induced changes in lipid metabolism leading to liver damage. Our findings highlight miRNAs potential for early disease detection and intervention in farm animal disease management, potentially reducing significant economic losses in agriculture. With only a fraction of miRNAs functionally characterized in farm animals, this review underlines more focused research on specific miRNAs altered in distinct diseases, using advanced technologies like CRISPR-Cas9 screening, single-cell sequencing, and integrated multi-omics approaches. Identifying specific miRNA targets offers a novel pathway for early disease detection and the development of mitigation strategies against mycotoxin exposure in farm animals.

A Study on Educational and Training Needs Assessment of Professionally Active Agronomists in the Area of Central Macedonia, Greece

The aim of this study was to investigate the educational and training needs of agronomists in the region of Central Macedonia in Greece, a region with significant contribution to the Greek agricultural economy. Data was collected through an electronic survey by a sample of 127 agronomists. A quantitative survey with a non-probability sampling was conducted, snowball sampling method was applied and descriptive tools were used to present the results in the form of means, percentages, standard deviations, analysis of variance and correlation analysis. Conclusions indicate differences in gender as they prioritize educational needs and a positive linear correlation between professional experience and the number of training programmes attended. High need of training declared in the subjects of Precision agriculture, Precision livestock farming & Use of new technologies. Low need for training declared in the subjects of Livestock production and farm animal disease control, Dairy farming, Protected agriculture, Floriculture management, Poultry farming and Fish farming technologies.

Pathomorphological changes in laboratory animals exposed to lethal doses of disinfec-tants

The use of disinfectants is a crucial aspect of preventive and health improvement measures for infectious diseases in farm and domestic animals. Regulatory documents require the determination of toxicity to macroorganisms, including the establishment of lethal doses and toxicity groups, during the development and registration of antimicrobial agents. This study aimed to investigate the histological changes in the internal organs of laboratory animals when determining lethal doses of innovative disinfectants. The experiments used domestic disinfectants containing glutaraldehyde as the active ingredient. Histological studies were conducted on the internal organs (kidney, liver, stomach, intestines, and spleen) of 75 laboratory animals using an Axioskop 40/40FL microscope (Carl Zeiss, Germany) with video microscopic photography. The methods used were under current standards. Hemodynamic disorders were observed in the renal tissue under the influence of lethal doses of aldehyde disinfectants. These disorders were characterized by capillary dilation and blood filling. Glomerular capillary dilation and overflow with blood cells were also detected. Additionally, stasis was observed in the lumen of the microcirculatory vessels throughout the entire length. The examination of histological sections from animal liver samples revealed a significant expansion of Dissé spaces, variations in the size of hepatocyte nuclei, beam decomposition and fragmentation, small acute perivascular hemorrhages, leukostasis in sinusoids, and hemodynamic disorders. The structure of the organ's beams was also disturbed, and a significant number of venous vessels were dilated and excessively filled with blood cells. Minor changes were detected in the stomach, including desquamation of the epithelial cells of the glands and their exfoliation into the gastric lumen, as well as circulatory disorders. Epithelial desquamation, blood vessel dilation, and signs of connective tissue edema were observed in the intestine. The kidneys exhibited signs of acute venous hemorrhage and stasis in vessels of various calibers, with the development of small acute parenchymal hemorrhages and localized lymphoid cell death in the white pulp. The prospect of further research is to investigate the histomorphological changes in the internal organs of laboratory animals when exposed to modern complex disinfectants with different active ingredients.

Aleutian disease: Risk factors and ImmunAD strategy for genetic improvement of tolerance in American mink (Neogale vison).

Aleutian disease (AD) is a devastating infectious disease in American mink (Neogale vison) industry caused by Aleutian mink disease virus (AMDV). Two crucial steps toward controlling infectious diseases in farm animals are: (i) assessment of the infection risk factors to minimize the likelihood of infection and (ii) selection of animals with superior immune responses against pathogens to build tolerant farms. This study aimed to investigate AD risk factors and evaluate a novel "ImmunAD" approach for genetic improvement of AD tolerance. Phenotypic records and pedigree information of 1,366 and 24,633 animals were included in this study. The risk of animal's age, sex, color type, and year of sampling on AMDV infection was assessed using a logistic regression model and counter immune-electrophoresis (CIEP) test results. ImmunAD phenotype was calculated based on AMDVG enzyme-linked immunosorbent assay (ELISA) and CIEP test results, and breeding values for ImmunAD were estimated using an animal model. Animals were classified into high-coordinated (HCIR), average-coordinated (ACIR), and low-coordinated immune responders (LCIR) using ImmunAD's breeding values, and the impact of selection of HCIR on live grade of pelt quality (PQ), harvest weight (HW), and harvest length (HL) breeding values were evaluated. Age of > 1 year, male sex, and year of sampling were identified as significant risk factors of AD (p < 0.05). A moderate-to-high heritability (0.55±0.07) was estimated for ImmunAD, while a higher heritability was observed among the CIEP-positive animals (0.76±0.06). Significantly higher breeding values were observed for PQ and HL among HCIR than those for LCIR and ACIR (p < 0.05). Our findings indicate the critical role of male breeders in AD distribution within mink farms. Regular screening of AD in male breeders before pairing them with females during breeding seasons can help disease control. ImmunAD strategy can be applied to genetic improvement of AD tolerance, with favorable impacts on some growth and production traits. Higher genetic gains can be achieved in populations with higher AD seroprevalences.

Occurrence of Salmonella in the environment

Salmonella is one of the most common causes of foodborne diseases. Although it is an intestinal bacterium, it can function perfectly well in various environments outside the host’s organism. Salmonella infections in humans are transmitted mainly through food of animal or plant origin and pose a serious problem for public health and the economy. Salmonella is an etiological factor of diseases in farm and free-living animals, which are also the most common source of environmental contamination. Reptiles and amphibians represent an important and often underestimated reservoir of these bacteria. Even under unfavorable conditions, the pathogen can survive for years in feed or food processing facilities mainly because of its ability to form biofilm. Salmonella can colonize plants epiphytically and endophytically. After penetrating external tissues, it can be transferred through the internal transport system to various organs, including fruit and seeds. This process depends on the serovar of the bacteria and the species of the plant being infected. The ability of Salmonella to survive and proliferate in the soil depends on many interacting factors, such as soil type, humidity, pH, temperature, and indigenous flora. Enriching the soil with natural fertilizers increases the occurrence of the pathogen and significantly reduces its rate of decline. In the aquatic environment, as well, Salmonella can survive for months after direct contamination with human or animal faeces or indirect contamination with insufficiently treated sewage sludge or runoff from agricultural land. The main determinants of Salmonella survival are temperature, sunlight, and the presence of accompanying microflora. Contaminated water used in processing can also be an indirect cause of transmission of intestinal pathogens to food.

Dangerous bacterial infections of farm animals in Russia and the Tyumen region

In the 21st century, there is a clear trend of growth in quantitative and qualitative indicators of Russian animal husbandry. The Tyumen region is distinguished by the sustainable development of the agro-industrial complex. In conditions of intensive development of animal husbandry in Russia and the Tyumen region, it is of particular importance to preserve the health of livestock at enterprises and in private subsidiary farms. The article examines particularly dangerous bacterial infectious diseases of farm animals. Such diseases include anthrax, tuberculosis, brucellosis and leptospirosis. The main epidemic characteristics in dynamics over the past five years, including the incidence of animals and the spread of infections in Russia and the Tyumen region, have been analyzed, peaks in morbidity and main trends have been identified. Brief characteristics of diseases are presented, the main preventive measures and measures provided for by legislation are analyzed.

The factors of the low iodine concentration in soil cover and drinking waters of the second ridge of the Mountain Crimea

Low iodine content in the environment can be one of the main factors of iodine deficiency diseases in humans and farm animals, including hypothyroidism, nodular thyroid tumors, irreversible brain damage in the fetus and newborn. Endemic goiter, caused primarily by iodine deficiency, is an urgent problem for most regions of modern Russia, including the Republic of Crimea. For the Republic of Crimea, along with other regions of the Crimean-Caucasian mountain zone, there is a deficiency of iodine in the lower links of the trophic chain, which provokes iodine deficiency diseases. The aim of the work was to reveal the contrast of iodine distribution in the lower links of the trophic chain: different types of soils for agricultural use and natural waters for drinking purposes from various sources in the Crimean Mountains. The article presents data of the iodine content in the soil cover and drinking water in the Crimean Mountains, on the example of the Bakhchisarai area (in Bodrak river valley). The dependence of the iodine content in the upper horizons of different types of soils, on the content of humus, pH-water soils and cation exchange has been established. It has been confirmed that the content of iodine in natural waters (springs, rivers, lakes) is determined primarily by the composition of water-bearing rocks.

The role of a universal microbiological ferment with probiotic action in changing the blood picture of calves in preweaning period

The use of probiotic feed additives is known in animal science and practice; they are an alternative way to replace antibiotics in animal husbandry. The use of probiotic drugs signifi cantly increases the natural resistance of the animal body, maintains a high level of immune status and reduces the risk of infectious diseases in cattle and farm animals in general. The purpose of the research was to study the eff ect of feeding a universal microbiological ferment with probiotic action Biolax-U in the diets of calves during the preweaning period in combination with a hygienic bedding desiccant Drytall Fresh. In order to conduct the research 2 groups (control and experimental) of clinically healthy calves of Black-and-White breed in the preweaning period per 15 heads in each were formed by the method of analogues, taking into account age, live weight and breed. Calves of the compared groups received milk and basic feed according to the calf feeding scheme used on the farm. At the same time, the calves of the experimental group additionally received the universal microbiological ferment with probiotic action Biolax-U per 10 ml each morning and evening feeding, i.e. in two steps. The use of Biolax-U ferment had the positive eff ect on improving the morphological composition and biochemical parameters of blood. A comparative blood test after 90 days of feeding calves in the experimental group with the probiotic ferment culture Biolax-U revealed the increase in the concentration of erythrocytes by 12,20 %, hemoglobin by 10,58 % compared to the beginning of the experiment, and by 7,26 and 11,02 %, respectively, compared to animals in the control group. It has been established that the use of the Biolax-U ferment in the diet of calves during the preweaning period in combination with the hygienic bedding desiccant Drytall Fresh enhanced redox processes in the body of calves, stimulated erythropoiesis, improved oxygen supply to tissues and organs, increased the intensity of protein metabolism and protective functions body.

Consumer Preferences and Attitudes towards Antibiotic Use in Food Animals.

Antimicrobial resistance (AMR) is one of the major challenges faced by society, with the real threat of the failure of many medical procedures. Antibiotics are also used in livestock production and provide a potential pathway to increasing AMR. The central challenge involves ensuring animal health and welfare while securing the long-term effectiveness of antibiotics. This paper reports the results of a survey of 5693 respondents from the customer panels of four major UK supermarkets regarding preferences and attitudes towards antibiotic use in food animals, and their perspectives on how the balance between animal welfare and human benefit can be achieved. The results of these surveys are consistent with those from other countries that found that consumers generally have limited knowledge about antibiotic use in agriculture and AMR, with around 50% responding "don't know" to many questions. There was agreement about the benefits of antibiotics outweighing harm, with 40% agreeing that, overall, the use of antibiotics to treat disease in farm animals delivers more benefit than harm. However, 44% neither agreed nor disagreed, indicating a high level of uncertainty and a situation that is potentially unstable. The seriousness of the AMR challenge is such that continued action for the more discriminating use of antibiotics must continue.

PATHOGENIC SPECIES OF CLOSTRIDIA AND THEIR ANTIBIOTIC RESISTANCE, VIRULENCE FACTORS, AND GENOMIC FEATURES

Scientific data on the diversity of dangerous clostridia species causing infectious diseases in farm animals are presented. Current knowledge about the pathogenicity and virulence factors of clostridia and their harmful effects on the organisms of highly productive animals. Information is provided on anaerobic bacteria that can form capsules and biofilm structures, which are important determinants of virulence that block the action of the immune systems of macroorganisms, antibacterial agents, and various disinfectants. Data on the phenotypic and molecular-genetic stability of such significant clostridia as C. perfringens and C. difficile, found in published research results, are presented in tables. Current information on the determinants of virulence identified in C. septicum, C. sordellii, C. sporogenes, and C. tetani from various biological materials from different animals is presented in the article. Mechanisms of resistance to antibiotics, altered expression of redox proteins, DNA repair, mutation genes responsible for antibiotic resistance, formation of biofilms, and the presence of a matrix that hinders the penetration of antimicrobial agents into bacteria are described, as well as their prevalence among pathogenic clostridia worldwide. Modern available therapy methods and antimicrobial agents are outlined as an alternative to therapeutic agents used to treat diseases in humans, animals, and poultry caused by clostridia.

ECONOMIC EFFICIENCY OF ANTI-EPIZOOTIC MEASURES, CARRIED OUT BY THE STATE VETERINARY SERVICE OF THE REPUBLIC OF SAKHA (YAKUTIA)

The article analyses the economic efficiency of anti-epizootic actions by the State Veterinary Service of the Republic of Sakha (Yakutia). The spread of infectious diseases of farm animals in the territory of Yakutia has a particular impact on the livestock economy. For 21 years (2001 - 2021), 99.5 thousand heads of all types of productive animals and poultry (cattle, pigs, horses, reindeer, small cattle, fur animals, poultry) fell ill with all infections. Furthermore, due to the death (case) and forced slaughter of sick animals, 31.2 thousand heads dropped out of the herd circulation. Diseases, including the death of animals, caused the loss of 188.9 tons of meat in live weight and 385 skins of fur-bearing animals, with a total economic loss of 426.6 million rubles. The economic efficiency of planned anti-epizootic measures in 2021 amounted to 870 million rubles. This efficiency is expressed through the indicator of the prevented economic damage for all farm animals. At the same time, the loss of 3054.9 tons of meat in live weight and 2.1 thousand skins of fur-bearing animals was prevented. The annual prevented economic damage was two times higher than the damage caused over 21 years, indicating the high economic efficiency of anti-epizootic measures. The share of prevented financial losses from infectious animal diseases in the cost of livestock products was 4.6%, which determines the contribution of livestock veterinary services to the economy of agricultural production in Yakutia.

EPIZOOTIC SITUATION OF HEMOPARASITIC DISEASES OF FARM ANIMALS IN IRKUTSK REGION

Background. Cattle anaplasmosis and babesiosis and theileriosis of horses are a group of natural focal vector-borne diseases that cause significant economic damage to livestock and horse breeding around the world. On the territory of the Baikal region these diseases and their pathogens remain little explored.&#x0D; Purpose – to study the epizootic situation of blood-parasitic diseases of farm animals in the Irkutsk region, to establish the species affiliation of the identified pathogens and their genetic diversity.&#x0D; Materials and methods. For the presence of Babesia spp./Theileria spp. and Anaplasma spp. has been researched659 horse blood samples, 579 sheep blood samples, 25 goat blood samples and 647 cattle blood samples. For the detection of babesia and theileria, nested two-round PCR with primers from the 18S rRNA gene region was used, and for the detection of anaplasma DNA with primers from the 16S rRNA gene region. For study the genetic diversity of the identified pathogens, selective sequencing of samples was carried out with subsequent phylogenetic analysis.&#x0D; Results. The DNA of Anaplasma spp. was detected in blood samples of small ruminants from 12 districts of the region. The infection rate of sheep and goats was 68.7±1.9% and 68%±9.3%, respectively. Nucleotide sequences of the 16S rRNA gene fragment were determined in 68 A. ovis samples from the blood of sheep and goats. The researched sequences were conservative for the studied gene and differed from each other in two heterozygous sites of the gene(T/C and G/A nucleotide substitutions).They corresponded to the sequences of the prototype strain Haibei (CP015994), as well as to the A. ovis sequences previously found in the blood of sheep from Altai (Russia) and Mongolia, deer and D. niveus and D. nuttalli ticks from China.&#x0D; DNA of Babesia spp./Theileria spp. found in blood samples of horses from 13 districts of the region. The average infection of horses was 64.2%±1.9%. Two etiological agents of equine piroplasmosis were found in the blood of the examined horses: T. equi (genetic groups A and E) and B. caballi. Definite sequences of the B. caballi 18S rRNA gene (700–1146 bp) were identical to each other and differed by at least ten substitutions and one deletion from the sequences available in the GenBank database. However, the greatest similarity was observed with B. caballi sequences found in the blood of a horse from Brazil (KY952238), ticks from Kazakhstan (MN907451) and China (MN173021).&#x0D; The results indicate that DNA of Anaplasma and Babesia in the blood of cattle have not been identified.&#x0D; Conclusion: The data received evidence a wide distribution of foci of small cattle anaplasmosis and piroplasmosis of horses in the Irkutsk region and the need to continue monitoring their epizootic condition.

Investigation Of the Composition of The Veterinary Preparation Trametin Plus

For the prevention and treatment of associated gastrointestinal and respiratory diseases in young farm animals, a new veterinary drug Trametin Plus is proposed, obtained on the basis of xylographic fungi using biotechnology methods. The properties of biological products obtained on the basis of microorganisms and fungi depend on the biologically active substances that make up their composition. In the literature, there is scattered information about the features of lipogenesis and the composition of fatty acids synthesized by wood-destroying fungi. The paper presents data on the study of the qualitative and quantitative composition of fatty acids, a qualitative analysis of volatile polar and non-polar organic compounds of the drug. It has been established that the total concentration of fatty acids is 70 µg/g of the preparation. Of these, 50.0% are free fatty acids, the esterified acids of which account for 50.0% of the total mass of fatty acids. Among the dominant non-polar volatile components of Trametin Plus, squalene can be noted. Organic aliphatic amino acids such as glycine, arginine, and β-alanine with a small molecular weight are presented as minor non-polar volatile components of Trametin plus. The analysis data confirm the multicomponent nature of the drug, which determines its various biological properties, namely, antibacterial, antiviral, antioxidant and immunostimulant activity, which determines the high therapeutic and prophylactic efficacy of the veterinary drug Trametin Plus.

Sequence analysis of TLR4 gene in river buffalo (Egyptian breed) and SNPs association with Mastitis

BackgroundMastitis is a major cause of economic loss for dairy farmers and industry. River buffalo plays an economically significant role in Egypt. Buffaloes with mastitis have reduced milk yield and change in milk composition. Genetic variations in the Toll-like receptor4 (TLR4) gene have been related to several diseases in farm animals and humans including mastitis. The present investigation aims to find the genotypic variations in the TLR4 gene and their relation to mastitis in the river buffalo, Egyptian breed.ResultsDNA from 30 buffaloes' blood samples (15 healthy and 15 with mastitis) were extracted and the TLR4 gene was sequenced. Twenty-one Single nucleotide polymorphisms (SNPs) were found from which four SNPs were associated with mastitis: one in 5'UTR (c.1-g27) and 3 SNPs in the coding region at c.87, c.575, and c.576. The nucleotide variations in SNPs c.1-g27(C > A) and c.87 (C > A) were only present in buffalo with mastitis, while buffaloes with genotype CC at both locations were healthy. The AA genotype at c.87 (P.29) results in a stop codon leading to an abnormally shortened protein. The nonsynonymous SNPs c.575 A > G and c.576 T > G shared amino acid 192 resulting in three amino acids (His192Arg/Gln). The dominant genotypes AA at c.575 and TT at c.576 were associated with mastitis resistance (OR < 1.00), while recessive genotype GG at c.575 was associated with mastitis susceptibility (OR > 1.00). These two SNPs may affect their role in ligand recognition since they were in the LRR4 domain (p.174–p.197) which is part of coreceptor binding region 1.ConclusionsThe present study confirms the relation between TLR4 genotypes and mastitis resistance or mastitis susceptibility in Egyptian dairy buffalo. The study suggested four SNPs (c1-g27C > A, c.87 C > A, c.575A > G, and c.576 T > G) have the potential to be markers for assisted buffalo selection to improve milk production.

Development of microbiological diffusion inhibition test for the determination of antibiotic residues in the milk

Antimicrobial agents are used in animal husbandry for the prevention and treatment of diseases in farm animals and have become an indispensable aspect of commercial livestock production. Antimicrobial therapy is well-established as a component of comprehensive preventive measures aimed at minimizing diseases in farm animals and has been incorporated into procedures directed at promoting livestock growth and productivity. Bacillus licheniformis strain T7 is susceptible to the antibiotics clidamycin, rifampicin, erythromycin, ciprofloxacin, tobramycin, tetracycline, penicillin, streptomycin, and chloramphenicol, has good sporogenic properties, grows rapidly on nutrient agar at 30-55 °C, pH 5.5–8.0, and can serve as a test culture in a microbial inhibitor test. Bacillus licheniformis T7 spores were obtained by growing the culture in Difco sporulation medium and then inactivating the vegetative cells at 90 °C for 20 minutes. Endospores of Bacillus licheniformis T7 co-polymerized with nutrient agar and germinated at 55 °C for 3–3.5 h, resulting in a pH shift from 5.5 to &gt;6.5, which can be measured with an acid-base indicator. Bromocresol purple was suggested as a pH indicator for use in a microbiological inhibition test, wherein the presence of antibiotics or other compounds impeding the development of a microbiological culture could be determined by a change in the agar's color. A microtube- and plate-based microbiological inhibitor test prototype has been developed.&#x0D;

DEVELOPMENT OF A METHOD FOR MANUFACTURING AND OBTAINING HYPERIMMUNE SERUM AGAINST STREPTOCOCCOSIS IN FARM ANIMALS

In the livestock farms of our country, infectious diseases are becoming more and more common in their etiology, the dominant role belongs to the opportunistic microflora. Streptococcus also belongs to this group of microorganisms [1].&#x0D; Streptococcosis is an infectious disease of young farm animals, characterized by severe septic phenomena, inflammation of respiratory system, gastrointestinal tract, joints.&#x0D; All types of young animals are susceptible to streptococcosis, but calves, lambs and foals get sick more often, starting from the first days of life, up to 3-4 months. The disease begins with a respiratory rate, the appearance of breath sounds and wheezing, and cough with the development of pneumonia. The first signs of the disease appear in foals at the age of 1.5-4 weeks. Clinical manifestations differ depending on the location and extent of metastatic abscesses. Mortality from complications reaches 10%. Factors contributing and predisposing to the emergence and development of this disease are inadequate balanced nutrition, violation of the rules for the care and maintenance of calves.&#x0D; The basis for the prevention of streptococcosis should be a complex of veterinary and sanitary measures and good nutrition. On dysfunctional farms, it is impossible to keep sick and recovered cows with newborns, as well as drink their colostrum and milk. In this regard, it is very important for veterinary medicine to investigate infectious diseases of cattle, as well as to develop effective means for protecting animals, improving measures related to the prevention and elimination of this disease[7,8].This work presents data on the isolation of a field isolate of bovine streptococcosis, an indication and identification of a bacterial culture. Bacterial mass obtained and prepared antigen for development hyperimmune serum. The scheme of immunization and obtaining hyperimmune serum on laboratory and industrial animals has been worked out. Carried out laboratory and industrial tests of hyperimmune serum.