Disease In Beef Calves Research Articles

Effect of aerosolized bacterial lysate on development of naturally occurring respiratory disease in beef calves.

BackgroundBovine respiratory disease (BRD) is a major problem affecting beef cattle after arrival to feedlots. Alternatives to antibiotics are needed for prevention.HypothesisStimulation of pulmonary innate immune responses at the time of arrival to a feedlot reduces the occurrence and severity of BRD.AnimalsSixty beef steers at high risk of BRD.MethodsRandomized, double‐blinded, placebo‐controlled study. Calves received saline or a lysate of Staphylococcus aureus and Escherichia coli by aerosol, at 16 hours after feedlot arrival. Calves were monitored for 28 days for disease outcomes and levels of Mycoplasma bovis and Mannheimia haemolytica in nasal swabs.ResultsDeath from M bovis pneumonia was significantly greater in lysate‐treated animals (6/29, 24%) compared to controls (1/29, 3%; odds ratio = 10.2; 95% confidence interval [CI] = 1.1‐96.0; P = .04). By 28 days after arrival, 29/29 lysate‐treated calves had ultrasonographic pulmonary consolidation compared to 24/29 control calves (P = .05). Lysate‐treated calves had lower weight gain compared to control calves (−8.8 kg, 95% CI = −17.1 to −0.5; P = .04), and higher body temperatures on days 4, 7, and 21 (0.19°C; 95% CI = 0.01‐0.37; P = .04). Nasal M bovis numbers increased over time and were higher in lysate‐treated calves (0.76 log CFU, 95% CI = 0.3‐1.2; P = .001).Conclusions and Clinical ImportanceAerosol administration of a bacterial lysate exacerbated BRD in healthy high‐risk beef calves, suggesting that respiratory tract inflammation adversely affects how calves respond to subsequent natural infection with M bovis and other respiratory pathogens.

Sources of variation of antimicrobial use in Charolaise and Limousine beef breeds in Veneto region (Italy)

Sources of variation of antimicrobial use in Charolaise and Limousine beef breeds in Veneto region (Italy)

Bovine respiratory disease in beef calves supported long transport stress: An epidemiological study and strategies for control and prevention

BRD is associated with infectious agents, but management and transport-stress are trigger factors. Metaphylactic administration of antimicrobial reduces colonization of respiratory tract by pathogens, but the development of antibiotic-resistance raises public health concerns leading to propose new control strategies. The study analyzed nasopharyngeal swabs of 231 imported cattle, 10% of 49 trucks, transported from France to southern Italy and, through Real-time PCR identified the prevalence of the involved pathogens speculating on strategies to reduce the impact of BRD. The samples were tested by Real-time PCR, for the detection of bovine coronavirus (BCoV), bovine respiratory syncytial virus (BRSV), bovine parainfluenza virus (BPiV), bovine adenovirus (BAdV), Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, and Mycoplasma bovis. Yates-corrected chi squared, or Fisher's exact test were used to compare both animal-health status and positivity/negativity to pathogens, and the relationship between presence/absence of clinical signs and Real-time PCR-positivity.H. somni and BCoV were the most frequently identified pathogens. In BRD-diagnosed cattle, BAdV was detected in 13.8% (19/138), BRSV in 14.5% (20/138) and BPiV in 4.3% (6/138). Healthy cattle were mostly positive for H. somni (89.2%, 83/93). A statistically significant association was observed between clinical signs and positivity to M. haemolytica (p value = 0.016).Although mass-medication and vaccination are used for BRD control, it still remains a primary health problem. Our results highlight that the nasopharyngeal microbiota could be affected by transport and that strategies to enhance calf immunity for reducing BRD-risk development would be more effective if applied at farm of origin prior to loading.

Effects of innate immune stimulation on naturally occurring respiratory disease in beef calves

Stress- and virus-induced immunosuppression are considered major risk factors for the development of bovine respiratory disease (BRD) in beef calves. Although vaccines and the metaphylactic use of antibiotics are available to minimize BRD in herds, it continues to be the leading cause of morbidity in feedlot calves. Research in mice found that lethal bacterial pneumonia was prevented by stimulating the innate immune system. The purpose of this study was to determine if stimulation of innate immune responses on arrival to a feedlot could decrease the prevalence and severity of naturally occurring BRD in beef calves.

Bovine neutrophils in health and disease.

Bovine neutrophils have similarities to those of other species with respect to mechanisms of their activation and migration into tissue, modulation of immune responses and the balance between microbial killing and host tissue damage. However, bovine neutrophils have biochemical and functional differences from those of other species, which may yield insights about the comparative biology of neutrophils. Neutrophils play protective and harmful roles in the infectious diseases of cattle that occur at times of transition: respiratory disease in beef calves recently arrived to feedlots and mastitis and other diseases of postparturient dairy cows. An important research focus is the mechanisms by which risk factors for these diseases affect neutrophil function and thereby lead to disease and the prospect of genetic or pharmacologic improvement of disease resistance. Further, in keeping with the One Health paradigm, cattle can be considered a model for studying the role of neutrophils in naturally occurring diseases caused by host-adapted pathogens and are thus an intermediary between studies of mouse models and investigations of human disease. Finally, the study of bovine neutrophils is important for agriculture, to understand the pathogenesis of these production-limiting diseases and to develop novel methods of disease prevention that improve animal health and reduce the reliance on antimicrobial use.

Evaluation of the effect of serum antibody abundance against bovine coronavirus on bovine coronavirus shedding and risk of respiratory tract disease in beef calves from birth through the first five weeks in a feedlot.

OBJECTIVE To evaluate the effect of serum antibody abundance against bovine coronavirus (BCV) on BCV shedding and risk of bovine respiratory disease (BRD) in beef calves from birth through the first 5 weeks in a feedlot. ANIMALS 890 natural-service crossbred beef calves from 4 research herds. PROCEDURES Serial blood samples for measurement of serum anti-BCV antibody abundance by an ELISA and nasal swab specimens for detection of BCV and other viral and bacterial BRD pathogens by real-time PCR methods were collected from all calves or subsets of calves at predetermined times from birth through the first 5 weeks after feedlot entry. Test results were compared among herds, over time, and between calves that did and did not develop BRD. The associations of various herd and calf factors with test results were also evaluated. RESULTS At the calf level, serum anti-BCV antibody abundance was not associated with BCV shedding, but BCV shedding was positively associated with BRD incidence before and after weaning. The mean serum anti-BCV antibody abundance at weaning for a group of calves was inversely related with the subsequent incidence of BRD in that group; however, the serum anti-BCV antibody abundance at weaning for individual calves was not predictive of which calves would develop BRD after feedlot entry. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that serum anti-BCV antibody abundance as determined with ELISA were not associated with BCV shedding or risk of BRD in individual beef calves from birth through the first 5 weeks after feedlot entry.

Bayesian evaluation of clinical diagnostic test characteristics of visual observations and remote monitoring to diagnose bovine respiratory disease in beef calves

Accurate diagnosis of bovine respiratory disease (BRD) in beef cattle is a critical facet of therapeutic programs through promotion of prompt treatment of diseased calves in concert with judicious use of antimicrobials. Despite the known inaccuracies, visual observation (VO) of clinical signs is the conventional diagnostic modality for BRD diagnosis. Objective methods of remotely monitoring cattle wellness could improve diagnostic accuracy; however, little information exists describing the accuracy of this method compared to traditional techniques. The objective of this research is to employ Bayesian methodology to elicit diagnostic characteristics of conventional VO compared to remote early disease identification (REDI) to diagnose BRD. Data from previous literature on the accuracy of VO were combined with trial data consisting of direct comparison between VO and REDI for BRD in two populations. No true gold standard diagnostic test exists for BRD; therefore, estimates of diagnostic characteristics of each test were generated using Bayesian latent class analysis. Results indicate a 90.0% probability that the sensitivity of REDI (median 81.3%; 95% probability interval [PI]: 55.5, 95.8) was higher than VO sensitivity (64.5%; PI: 57.9, 70.8). The specificity of REDI (median 92.9%; PI: 88.2, 96.9) was also higher compared to VO (median 69.1%; PI: 66.3, 71.8). The differences in sensitivity and specificity resulted in REDI exhibiting higher positive and negative predictive values in both high (41.3%) and low (2.6%) prevalence situations. This research illustrates the potential of remote cattle monitoring to augment conventional methods of BRD diagnosis resulting in more accurate identification of diseased cattle.

Association of hematologic variables and castration status at the time of arrival at a research facility with the risk of bovine respiratory disease in beef calves

To determine the association of CBC variables and castration status at the time of arrival at a research facility with the risk of development of bovine respiratory disease (BRD). Retrospective cohort study. 1,179 crossbred beef bull (n = 588) and steer (591) calves included in 4 experiments at 2 University of Arkansas research facilities. Calves underwent processing and treatments in accordance with the experiment in which they were enrolled. Castration status and values of CBC variables were determined at the time of arrival at the facilities. Calves were monitored to detect signs of BRD during a 42-day period. The areas under the receiving operator characteristic curves for CBC variables with significant contrast test results ranged from 0.51 (neutrophil count) to 0.67 (eosinophil count), indicating they were limited predictors of BRD in calves. The only CBC variables that had significant associations with BRD in calves as determined via multivariable logistic regression analysis were eosinophil and RBC counts. The odds of BRD for bulls were 3.32 times the odds of BRD for steers. Results of this study indicated that low eosinophil and high RBC counts in blood samples may be useful for identification of calves with a high risk for development of BRD. Further research may be warranted to validate these variables for prediction of BRD in calves. Calves that were bulls at the time of arrival had a higher risk of BRD, versus calves that were steers at that time.

Increased annexin A1 and A2 levels in bronchoalveolar lavage fluid are associated with resistance to respiratory disease in beef calves

Strategies to control bovine respiratory disease depend on accurate classification of disease risk. An objective method to refine the risk classification of beef calves could be economically beneficial, improve welfare by preventing unexpected disease occurrences, refine and reduce the use of antibiotics in beef production, and facilitate alternative methods of disease control. The objective of this study was to identify proteins in bronchoalveolar lavage fluid (BALF) of stressed healthy calves that predict later disease outcome, serve as biomarkers of susceptibility to pneumonia, and play a role in pathogenesis. BALF was collected from 162 healthy beef calves 1–2 days after weaning and transportation. Difference in gel electrophoresis (DIGE) and mass spectrometry were used to compare proteins in samples from 7 calves that later developed respiratory disease compared to 7 calves that remained healthy. Calves that later developed pneumonia had significantly lower levels of annexin A1, annexin A2, peroxiredoxin I, calcyphosin, superoxide dismutase, macrophage capping protein and dihydrodiol dehydrogenase 3. Differences in annexin levels were partially confirmed by western blot analysis. Thus, lower levels of annexins A1 and A2 are potential biomarkers of increased susceptibility to pneumonia in recently weaned and transported feedlot cattle. Since annexins are regulated by glucocorticoids, this finding may reflect individual differences in the stress response that predispose to pneumonia. These findings also have implications in pathogenesis. Annexins A1 and A2 are known to prevent neutrophil influx and fibrin deposition respectively, and may thus act to minimize the harmful effects of the inflammatory response during development of pneumonia.

Association Between Serum Mineral and Vitamin A and E Concentration and Respiratory Disease in Beef Calves Entering Backgrounding Lots

The objectives of this study were 1) to measure morbidity and mortality rates attributable to respiratory disease in beef calves originating from southwestern Utah, 2) to evaluate herd of origin vitamin/mineral serum concentration at backgrounding lot entry, and 3) to evaluate a potential association between serum concentrations of 29 minerals and vitamins A and E at backgrounding lot entry with respiratory disease in recently weaned beef calves. This was a prospective longitudinal study with matched case-control and cross-sectional studies nested within the main study design. Pen-specific morbidity rates attributable to respiratory disease ranged from 1.2% to 81.8%. Serum concentrations of vitamins A and E in calves sampled (n=224) from eight southern Utah ranches were commonly less than published low-normal values. In these data, there was no detectable association between serum vitamin A and E or mineral imbalances and respiratory disease. Herds that experience a high rate of bovine respiratory disease (BRD) should be evaluated for mineral and vitamin deficiencies and supplemented as needed. If not deficient, it should not be expected that supplementation would reduce the rate of BRD in the face of mismanagement.

Genetic analysis of bovine respiratory disease in beef calves during the first year of life.

The prevalence of bovine respiratory disease (BRD) was assessed in a population of 10,142 beef calves representing nine pure breeds and three composite populations born in 1983 through 1988. Twenty-four percent of the calves experienced at least one episode of respiratory disease during the 1st yr of life; frequencies over the six birth years ranged from 14 to 38%. The timing of respiratory disease outbreaks differed among birth years; in 4 of the 6 yr, more illness occurred in the pasture before weaning than in the feedlot after weaning. Frequencies of BRD during preweaning and postweaning periods were analyzed separately. Pure breeds and composite populations within a single preweaning location differed in frequency of illness during the preweaning period. However, not all possible breed comparisons could be made because preweaning location differed for the breed groups, and preweaning location had a significant effect on the frequency of respiratory disease in the preweaning period. The preweaning location effect did not carry through to the postweaning period. Pinzgauers had the highest BRD frequency within the feedlot (24.6%). The heritability estimates of BRD during the preweaning and postweaning periods did not differ significantly from 0 (.10 +/- .02 and .06 +/- .07, respectively). Although it is likely that response to selection for resistance to BRD would be slight using the animal's history of BRD as the selection criterion, including information on relatives or additional immune traits may improve the accuracy of an estimated breeding value for BRD resistance.

Effect of vaccination against bovine myxovirus parainfluenza-3 before weaning and at the time of weaning on the natural history of respiratory disease in beef calves.

Earlier reports have described acute respiratory disease in native beef calves after weaning and attempts at prevention by inactivated bovine parainfluenza-3 (SF-4) tissue culture vaccines with and without an adjuvant (1, 2). The present report consists of a comparison of the effects of one and two doses of SF -4 adjuvant vaccine• in vaccinated and unvaccinated calves. Preparation of the vaccine was described previously (2).