Digestive System Disorders Research Articles

Supply and Consumption Methods of Medicinal Plants in Mazar-e-Sharif City, Afghanistan

Background: Balkh province is located in the northern part of Afghanistan, which is rich in medicine plant diversity. The large number of medicinal plant shops (Attari) in the center of Mazar-e-Sharif city indicates that the citizens of Balkh province are familiar with medicinal plants and their uses. In this study, an attempt has been made to investigate the socioeconomic characteristics, medicinal plant distribution patterns, consumption methods, and types of medicinal plants in the Mazar-e-Sharif city Attaris. Materials and Methods: The study focused on 102 Attaris in the research area, research factors such as the age of medicinal plant sellers, monthly income, education level, marital status, number of people working in Attaris, and work experience of Attaris were studied. Findings: The results revealed that the average age of people in Attaris is 49 years with a work experience of about 15 years, and only 3% have an education related to medicinal plants. About 20 % of medicinal plant sellers had a university education but not related to medicinal plants science, about 17 % had a diploma, 30 % had a high school education and the remaining 12 % had primary education. Unbelievably, about 50 % of consumers were rich people and the remaining 50 % of the clients were low-income. People visited Attari's to heal their digestive system disorders are the most common health issues, followed by issues with the kidneys, bladder, and urinary system, respiratory conditions, skin and wound issues, hypertension and cardiovascular conditions, liver issues, diabetes, typhoid, pain, helminthiasis, anorexia, gynecological complaints, sexual dysfunction, weakness, fever, and obesity. Lemon balm, Common jujube, Starflower, Black pepper, Cumin, and Turmeric were the high-selling medicinal plants offered in Mazar-e-Sharif Attaris'. Conclusion: A large number of medicinal plant shops (Attaris) in the center of Mazar-e-Sharif city indicates that the citizens of Balkh province are familiar with medicinal plants and their uses. However, unfavorable economic situation of some people who referred Attaris showed that the existence of traditional medicine systems (Attaris) is considered to be one of the urgent needs of the Mazar-e-Sharif.

Analysis of Adverse Drug Reactions to First-Line Anti-Tuberculosis Drugs Using the Korea Adverse Event Reporting System

BackgroundAdverse drug reactions (ADRs) to first-line anti-tuberculosis (TB) drugs are common; however, there have been few reports of nationwide epidemiologic studies on ADRs to anti-TB drugs in Korea. This study aimed to investigate the clinical characteristics of various ADRs to first-line anti-TB drugs using a nationwide database of ADRs.MethodsWe used the Korea Adverse Event Reporting System (KAERS) database (2009–2018). The study subjects were selected using the Korean Standard Classification of Diseases codes for pulmonary and extrapulmonary TB and electronic data interchange codes for isoniazid (INH), rifampicin (RIF), ethambutol (ETB), and pyrazinamide (PZA). The causality assessment of “possible,” “probable,” or “certain” by World Health Organization-Uppsala Monitoring Center System causality category was selected.ResultsA total of 1,562,024 ADRs were reported in the KIDS-KAERS database from 2009 to 2018, where ADRs to first-line anti-TB drugs were 17,843 cases (1.14%). The most common causative drugs were RIF (28.7%), INH (24.0%), ETB (23.4%), and PZA (23.9%) in that order. 48.5% of cases were reported in the older patients (≥ 60 years). According to organ system, gastro-intestinal system disorder was most common (32.0%), followed by skin and appendage (25.9%), liver and biliary system (14.2%). Nausea was the most common ADR (14.6%), followed by hepatic enzyme elevation (14.2%), rash (11.7%), pruritus (9.1%), vomiting (8.9%), and urticaria (4.2%). Most ADRs appeared within 1 month, but ADRs such as neuropathy, paresthesia, hematologic abnormalities, renal function abnormalities and liver enzyme abnormality were also often reported after 2 months.ConclusionOur data are clinically informative for recognizing and coping with ADRs of anti-TB drugs.

Evaluation of Pre-Diagnosis Dietary Habits of Patients with Colorectal Cancer

Purpose: The aim of this study was to evaluate the effects of lifestyle and obesity on colorectal cancer patients. Material and Methods: The study was conducted on 40 patients with colorectal cancer (Patient Group- PG) who received diagnosis in the past 6 months and 40 volunteer adult controls (Control Group- CG) who did not have any digestive system disorders and/or diagnosis of cancer. The questionnaire form, which was developed in order to determine the lifestyle and dietary habits of the individuals, was completed by the patients and the individuals in CG. Anthropometric measurements were taken by the researcher. Results: The general mean age of the individuals was 59.8 ± 10.3 years in the PG and 59.6 ± 10.6 years in the CG. It was found out that CRC history was present in 10% of the individuals with CRC and the history of other cancer types was present in the families of 32.5% of the individuals with CRC. The pre-diagnostic body weights of the individuals in the patient group were found to be higher than the body weights during the study. Sunflower oil consumption is 87.5% among the individuals in the PG and 67.5% among the individuals in the CG (p<0.05). The consumption of ready-made soups and meat and chicken bouillon is 37.5%, 40.0% in the PG and 10.0% and 12.5% in the CG, respectively (p<0.05). Conclusion: It can be said that there is a relationship between colorectal cancer and lifestyle factors, and that changes may result in a reduced risk of developing colorectal cancer.

Comparative analysis of population mortality in the cities of Severodvinsk and Arkhangelsk

Increasing use of ionizing radiation sources in different spheres of human life dictates the need for investigating the effects of low-dose radiation on mortality and morbidity. The aim of this study was to compare mortality from the most common non-communicable diseases in the cities of Severodvinsk and Arkhangelsk. We analyzed the rates of age- and sex-specific mortality from circulatory system diseases (CSD), malignancies, digestive system disorders, respiratory system diseases, and external causes. CSD-related mortality among men and women past working age was higher in Severodvinsk than in Arkhangelsk (median (Q1; Q3): 3,349 (3,271; 3,458) vs 2,651 (2,618; 2,756), p &lt; 0.012; 1,947 (1,890; 2,022) vs 1,753 (1,727; 1,809), p &lt; 0.012; 292 (281; 342) vs 265 (253; 274), p &lt; 0.025, respectively). For other causes of death, mortality rates in Severodvinsk did not exceed those in Arkhangelsk. Increased mortality from CSD in Severodvinsk cannot be linked to socioeconomic conditions or chemical air pollution because the standard of living is higher in Severodvinsk than in Arkhangelsk, whereas the level of chemical pollution is lower. At the same time, the presence of the nuclear shipyard and radioactive waste repository in Severodvinsk could cause chronic exposure to low-dose radiation. It is important to expand preventive measures aimed at early detection of vascular damage in nuclear workers and general groups of population residing in the vicinity of hazardous radiation sites.

TPH1 gene polymorphism rs211105 is associated with serotonin and tryptophan hydroxylase 1 concentrations in acute pancreatitis patients

BackgroundThe role of serotonin and its metabolic pathway in proper functioning of the pancreas has not been thoroughly investigated yet in acute pancreatitis (AP) patients. Tryptophan hydroxylase (TPH) as the rate-limiting enzyme of serotonin synthesis has been considered for possible associations in various diseases. Single-nucleotide polymorphisms (SNPs) in TPH genes have been already described in associations with psychiatric and digestive system disorders. This study aimed to explore the association of a rs211105 (T/G) polymorphism in TPH1 gene with tryptophan hydroxylase 1 concentrations in blood serum in a population of acute pancreatitis patients, and to investigate this association with acute pancreatitis susceptibility.ResultsOur data showed an association between the presence of the T allele at the position rs211105 (OR = 2.47, 95 % CI 0.94–6.50, p = 0.06) under conditions of a decreased AP incidence. For TT and GT genotypes in the control group, the lowest concentration of TPH was associated with higher serotonin levels (TT: Rs = − 0.415, p = 0.0018; GT: Rs = − 0.457, p = 0.0066), while for the AP group the highest levels of TPH among the TT genotype were associated with lower levels of serotonin (TT: Rs = − 0.749, p < 0.0001, and in the GG genotype higher levels of TPH were associated with higher levels of serotonin (GG: Rs = − 0.738, p = 0.037).ConclusionsHere, a new insight in the potential role of a selected genetic factor in pancreatitis development was shown. Not only the metabolic pathway of serotonin, but also factors affecting serotonin synthesis may be interesting and important points in acute pancreatitis.

A system for reporting and evaluating adverse drug reactions of herbal medicine in Taiwan from 1998 to 2016

The Taiwan Adverse Drug Reaction Reporting System for Herbal Medicine (TADRRS-HM) has systematically documented suspected adverse events from adverse drug reaction (ADR) reports from 1998 (prior to its formal establishment in 2001) and evaluates safety profiles of herbal medicines. This article describes findings from 2079 ADR reports filed between 1998 and 2016: 941 reports involved single herbs and 87 involved folk herbals; 842 were generated from clinical trials, while 209 ADR reports involving foods, health foods, dietary supplement foods and herbal cuisine were grouped as Other. Severity assessments using the Modified Hartwig and Siegel scale classified 72.4% of ADRs as mild, 17.4% as moderate and 6.5% as severe. System Organ Class classification of the ADRs identified gastrointestinal system disorders as the most common (33.4%), followed by skin and subcutaneous tissue disorders (21.2%). The TADRRS-HM records indicate that herbal medicines may cause a wide range of ADRs. Aconiti Radix, Xiao-Qing-Long-Tang, and Datura suaveolens were the most commonly reported single herb, herbal formula, and folk herbal, respectively. The data indicate that herbal medicines may cause a wide range of ADRs. This system will confer long-term benefits for the development of Taiwan’s herbal medicines adverse reaction database and facilitate epidemiological analysis.

Intussuscepção cecocólica e torção de ceco em bovinos

This work aims to describe the clinical, laboratory, and anatomopathological findings of two bovines, one affected by cecocolic intussusception, and the other by dilation with cecal torsion. The clinical examination demonstrated metallic resonance from the right flank, ruminal and intestinal hypomotility, and abdominal distension, in addition to alterations in feces characteristics. Was observed in the two animals leukocytosis by neutrophilia with regenerative shift to the left, and hyperfibrinogenemia. The analysis of ruminal fluid revealed impairment of the microbiota and an increase in chloride levels. The laparoscopic examination performed on one of the animals showed dilation of the colon, the cecum with hyperemia and serous edema, with a dividing halo between affected and unaffected portions, in addition to reddish peritoneal fluid. In the laparotomy, an enlarged cecum was found, with gaseous and liquid contents, swollen and turgid colon, and peritonitis. In addition to the findings observed during surgery, the anatomopathological examination demonstrated, in bovine 01, intestinal intussusception in the region of the cecocolic valve, and, in bovine 02, twisting of the loop at the ileocecocolic junction. Despite the low occurrence of digestive system disorders in cattle, cecal torsion and intussusception represent serious intestinal clinical conditions. These reports take the attention to the importance of a multidisciplinary approach to provide a correct diagnosis of intestinal diseases.

The Use of Medicinal Plants in Digestive System Related Disorders- A Systematic Review

The most prevalent forms of ailments affecting a huge number of individuals throughout the world are digestive system problems. According to the World Health Organization (WHO), over 100 million people died globally as a result of digestive system diseases in 2012. Despite this, most scientific bodies disregard the relevance of a healthy digestive system and associated problems in health debates. Food and lifestyle have a direct influence on digestive health, and a healthy diet or a change in lifestyle can address a variety of issues. Plants provide a variety of phytochemicals and nutraceuticals that may aid in the treatment and prevention of a number of digestive problems. Plant-based remedies, including spices, are widely used by ordinary people across the world and can help digestion without causing adverse effects. Numerous ethnomedicinal studies show that the use of medicinal plants by traditional populations to treat digestive problems is widespread across the world. The biological activities of traditional herbal medicine used to treat digestive problems have been studied and generally indicate minimal side effects. However, as the demand for herbal medicines derived from plants grows across the world, more comprehensive clinical research on the advantages and toxicity of herbal medicines should be conducted. The goal of this study is to provide examples of research that have established and verified the use of plant-based medicines to treat digestive problems. In particular, traditional medicinal plant extracts or phytochemicals that have been shown to reduce symptoms of digestive system disorders along with mode of action and safety will be discussed.

Клінічні прояви гастроінтестинальної форми харчової алергії у дітей і підходи до її діагностики

Гастроінтестинальна харчова алергія обумовлена розвитком алергічного запалення в слизовій оболонці шлунково-кишкового тракту, механізмами якого є як імуноглобулін E (IgE)-опосередковані (оральний алергічний синдром, негайна гастроінтестинальна гіперчутливість.), так і не-IgE-опосередковані (білок-індукований ентероколітичний синдром, білок-індукована ентеропатія, білок-індукований алергічний проктоколіт) і змішані, IgE- та не-IgE-опосередковані реакції (еозинофільний езофагіт, еозинофільний гастрит, еозинофільний гастроентерит). Гастроінтестинальні прояви харчової алергії також поєднуються із симптомами атопічних захворювань, частіше з атопічним дерматитом, кропив’янкою й ангіонабряком. Клінічні прояви алергічного ураження шлунково-кишкового тракту різноманітні та не є специфічними. До загальних ознак гастроінтестинальної алергії належать: блювання (виникає від декількох хвилин до 4–6 годин після прийому їжі); коліки (одразу чи через декілька годин після вживання їжі); запори; діарея; відмова від їжі (від конкретного продукту або взагалі від прийому їжі); абдомінальний біль; метеоризм, наявність слизу та еозинофілів у випорожненнях; зниження апетиту; головний біль. Зважаючи на неспецифічність клінічних проявів гастроінтестинальної харчової алергії, диференціальну діагностику слід проводити з такими захворюваннями, як захворювання й аномалії розвитку травної системи, психічні та метаболічні порушення, інтоксикації, інфекційні хвороби, недостатність ендокринної функції підшлункової залози, целіакія, муковісцидоз, імунодефіцити, дисахаридазна недостатність, побічна дія лікарських засобів, ендокринна патологія, синдром подразненого кишечника. Існуючі методи діагностики гастроінтестинальної алергії є обмеженими та недосконалими, що вимагає подальших наукових пошуків і досліджень, спрямованих на вчасне виявлення цієї патології, профілактику виникнення у генетично схильних дітей, розробку оптимальних алгоритмів діагностики, запобігання прогресуванню клінічних проявів, вибору індивідуальної дієтотерапії й адекватного медикаментозного лікування. В огляді літератури наведені сучасні погляди щодо діагностики гастроінтестинальної харчової алергії у дітей. Розглянуто клінічні прояви та діагностичні підходи до окремих видів гастроінтестинальної алергії залежно від механізму їх виникнення.

Detergent waste treatment through the modification of biofilter reactors

The use of detergent in households is practiced by almost all people. These conditions make the quality of the environment, especially water bodies, more disturbed when the detergent waste disposal without prior processing. Hazard by detergent can interfere with human health such as digestive system disorders, skin irritation, and even one of the causes of cancer. The aimed of this research was to design a modified biofilter reactor to reduce levels of detergent and other organic substances in domestic wastewater. This research was a pre-experimental design research with designs used is pre-test, treatment, and post-test. The research media used were bioball because bioball has more space on its surface for bacterial growth that will form bacterial phylum, while activated carbon serves to increase efficiency in reducing levels of organic matter and to reduce the concentration of surfactants dissolved. The process is more effective if given a treatment with a process of coagulation, flocculation, and sedimentation. The results showed a decrease in detergent content from 14.56 mg/L to 0.86 mg/L (94.1%). These results indicate that the Bio Filter reactor was effective in reducing detergent levels to meet the detergent waste standard requirements based on East Java Governor Regulation No. 72/2013.

Ethnobotanical and agronomical survey of Brocchia cinerea (Delile) Vis. plant used by people in the Figuig and Draa-Tafilalet regions, Morocco

Context: As part of the development of the natural heritage of Morocco, an ethnobotanical and agronomic study was conducted in the regions of Figuig and Draa- Tafilalet. Aims: To collect detailed information about the usage of Brocchia cinerea plant in human therapy and its incorporation into the feed system of livestock. Methods: The survey was carried out over five months, using semi-structured and structured interviews. A total of 197 interviews with traditional health practitioners and educated villagers were conducted. Results: Interview results showed that Brocchia cinerea is used in indigenous pharmacopeia to alleviate common diseases symptoms of the digestive system (36%), nervous system (19%), respiratory system (17%), ear, nose and throat (ENT) disorders (8%), and rheumatism (7%). The aerial parts of this plant were the most commonly used (36%), and the most preferred method for the preparation of traditional drugs was a decoction (33%). The results obtained from the agronomical survey show that the milk yield and meat production in ruminants noticeably increase after consumption of this plant. Conclusions: The data collected may help prevent the loss of traditional knowledge on the use of plants characterizing the ecosystem of these study areas and may constitute preliminary information required for a future phytochemical study on the plants most frequently used. The agronomic survey carried out can help the range managers improve the forage quality by using Brocchia cinerea for the betterment of animal health and productivity.

Functional state of the digestive organs in patients with celiac desease associatedFunctional state of the digestive organs in patients with celiac desease associated with connective tissue dysplasia with connective tissue dysplasia

The article presents the results of our own research devoted to the study of the functional characteristics of the digestive system in patients with celiac disease associated with connective tissue dysplasia (CTD).Materials and methods. The study included 90 patients from the register of adults with celiac disease. To assess CTD, patients underwent clinical and laboratory- instrumental examination, including ultrasound of the abdomen organs, kidneys and heart, EGDS. The assessment of the functional state of the gastrointestinal tract was carried out according to the ultrasound data of the abdomen organs, coprogram and PEGEG.Results. The results of the study showed that all patients had signs of CTD with involvement of at least two organ systems. The most common visceral stigmas of dysplasia were MVP (66.7%), gallbladder anomalies (53.3%), tracheobronchial dyskinesia (35.6%) and signs of nephroptosis (30.0%). Anomalies of the gallbladder included single bends in the body area — 26 people (28.9%) and in the neck area –5 people (5.6%), and double bend — S-shaped gallbladder — 17 people (18.9%). At the same time, an increase in the proportion of gallbladder anomalies was revealed as the severity of CTD increased, with significant differences in the groups in patients with an S-shaped gallbladder (p&lt;0.05), which correlated with biliary sludge and signs of maldigestion. According to PEGEG data, an increase in tonic activity (Pi / Ps) at the frequency of the duodenum with an increase in the degree of CTD was revealed, with the maximum values in group 3 patients being 3.84 ± 1.03, which reflects the presence of hypertensive dysfunction.Conclusion. The functional state of the digestive system in celiac patients with signs of CTD is characterized by disorders of the gastroduodenal complex and the biliary system. A high degree of CTD in celiac disease patients should be considered as an independent risk factor for motor disorders of the upper gastrointestinal tract and biliary system, which must be taken into account when carrying out therapeutic and prophylactic measures.

Ethnomedicinal study of plants used in the Uvira Territory (Democratic Republic of Congo)

This study aimed to investigate the ethnomedicinal plant knowledge among people living in The Uvira Territory. The data were collected using semi-structured interviews with and field observation in seven villages. The ethnomedicinal data was analyzed using the informant consensus factor (ICF), family importance value (FIV), and Jaccard index (JI). Sixty-nine medicinal plants belonging to 61 genera and 34 families were used to treat eight disease categories. Fabaceae was not only the dominant family but also a family with the high FIV. Decoction and pound were the most common methods of preparation, while leaves were the most used part. We compared this study with 24 other ethnomedicinal studies conducted in RD Congo and neighboring countries, and the results showed that the Jaccard index ranged from 0.57 to 10.94. The highest degree of similarity (10.94) was found with another study conducted in Congo, while the lowest degree of similarity (0.57) was found with a study conducted in Rwanda. The disease category for which there was the highest number of use (66) and plant species (39) was “diseases of the digestive system disorders and intestinal parasites” (ICF 0.42). The investigation of the plants used as drugs in the study area revealed that the population daily relies on medicinal plants to treat different diseases.

Abstract 2686: Preventive effects of saffron in a colitis mouse model

Abstract Background: Inflammatory bowel disease (IBD) is a chronic inflammation of the gastrointestinal tract with rising incidence and no effective cure. IBD increases the risk of colon cancer through a Colitis-Associated-Cancer (CAC) pathway. Despite available anti-inflammatory or immunosuppressive drugs, many patients fail or loose response over time and many others experience drug-induced adverse events. Natural plant products are increasingly used by IBD patients and reported to have some efficacy for IBD in experimental models and clinical trials. Saffron (Crocus sativus), has been reported to play a key role in treatment of different digestive system disorders but its preventive role in IBD has not been explored. Aim: To establish whether saffron treatment modulates immunity in intestinal inflammation and ameliorates experimental colitis. Methods: Mice were pre-treated with either saffron (10 or 20 mg/kg body weight) or vehicle through daily oral gavage for 4 days before dextran sulfate sodium (DSS) administration. Then, we induced acute colitis in C57BL/6 mice with 2.5% DSS. On day 11, mice were euthanized and analyzed for gross and microscopic inflammation. Distal colon segments were collected for protein expression from the colon for immunophenotyping using ELISA. TNF-a, IL-b, and IL6 expression was analyzed by immunoblot analysis. Results: Saffron Pre-treatment improved gross and histopathological characteristics of colonic mucosa in experimental colitis mice. Saffron dose 10 and 20 mg/kg body weight showed a significant improvement in body weight, disease activity index (DAI), colon length, and histology score, when compared to vehicle treated DSS mice group. Immunoblot analysis showed saffron pre-treatment significantly decreases pro-inflammatory TNF-a, IL-b, and IL-6 in the colon tissues indicating saffron mediates its preventive effect through anti-inflammatory pathways. Conclusion: These data suggest saffron therapeutic potential of saffron and its preventive role in treating experimental colitis in part via immune response modulation and a switch from pro- to anti-inflammatory profile. The study also gives insights in developing an alternative treatment for IBD-associated colitis. Citation Format: Suhrid Banskota, Waliul Khan, Gulshan Singh, Aida Habtezion, Hassan Brim, Hassan Ashktorab. Preventive effects of saffron in a colitis mouse model [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2686.

Large Scale Internet-based Survey of Patients With a Myeloproliferative Neoplasm: Opinions and Experiences Regarding SARS-CoV-2 (COVID-19) Vaccination Strategies in 2021.

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Clinical and immunological peculiarities of parasitic infections in children with digestive system disorders in Western Ukraine

Clinical and immunological peculiarities of parasitic infections in children with digestive system disorders in Western Ukraine

ETHNOBOTANICAL STUDY OF DIGESTIVE SYSTEMS DISORDERS IN BADUY ETHNIC, INDONESIA

Digestive disorders rank among the most common problems faced in Indonesia, especially for the Baduy people in Banten Province. Although the Baduy live in water-rich areas, their lack of sanitation facilities and unawareness of methods of disease prevention have prompted high morbidity and mortality rates in their communities, largely due to digestive system disorders that they continue to treat with medicinal plants. This survey was undertaken to document Baduy indigenous medicinal plants that were used to treat and prevent different types of digestive system disorders in their communities using quantitative ethnobotanical approaches. Ethno medicinal data were collected from 30 informants regarding their knowledge on medicinal plants. Quantitative approaches were used to determine the use value and informant consensus factor values of collected data. The results revealed that the Baduy currently use 54 medicinal plant species belonging to 30 families to treat digestive system disorders. Additional research is required, however, to validate the function of the medicinal plants and identify their active compounds.

Роль поліморфізму rs_7927894 гена FLG та загального IgЕ у прогнозуванні клінічних фенотипів атопічного дерматиту в дітей

Atopic dermatitis (AD) is the most common chronic allergic disease of childhood, the pathogenesis of which is based on endogenous genotype and which manifests by various clinical phenotypes — isolated or combined with other forms of atopy — allergic rhinitis/rhinoconjunctivitis (AR/ARC) and/or bronchial asthma (BA). Currently, one of the most studied genetic markers of AD developmental risk is the single nucleotide polymorphism of the filaggrin gene (SNP FLG), in particular, rs_7927894. The basic AD biomarker is total serum immunoglobulin E (IgE). But, so far, there has been no studies combining the mentioned predictors markers within different clinical AD phenotypes in children. Purpose — to detect the variants of SNP rs_7927894 of FLG gene and serum total IgE concentrations as personalized predictors panel for different AD clinical phenotypes developmental risk in children. Materials and methods. There were recruited 2 groups of patients into the study: the main comprised 39 children with phenotypes of AD isolated and combined with AR/ARC and/or BA; the control group comprised 47 children with disorders of digestive system (functional dyspepsia, chronic gastritis, peptic ulcer, functional biliary disorders) without clinical signs of atopy. The threshold level of statistical significance was set as p&lt;0.05. Results. There were detected the predictor genotype and biomarker for the AD developmental risk as per AD isolated phenotype: 4.11 (95% CI 1.28; 13.18, p&lt;0.05) within C/T SNP rs_7927894 of FLG gene variant and 8.98 (95% CI 2.53, 31.86, p&lt;0.001) for total serum IgE&gt;173 IU/ml. As well, predictor genotype and biomarker for the developmental risk of the AD combined with AR/ARC/BA phenotype were detected: 2.88 (95% 1.07; 8.54, p&lt;0.05) within the C/T SNP rs_7927894 of FLG gene variant and 8.98 (95% CI 2.53; 31.86, p&lt;0.001) for total serum IgE&gt;213 IU/ml. Additionally, the developmental risk for the phenotype of AD combined with AR/ARC/ BA in comparison with AD isolated at a cut-off serum total IgE&gt;1001 IU/ml was detected as 16.00 (95% CI 2.68; 95.44, p&lt;0.01). Conclusions. The C/T SNP rs_7927894 of FLG gene variant and cut(off serum IgE concentrations are significantly associated with the developmental risk of AD clinical phenotypes in children. Total IgE remains a significant predictor biomarker of AD risk in children aged 3 to 18 years at serum concentrations &gt;173 IU/ml for the AD isolated and at serum concentrations &gt;213 IU/ml for the AD combined with AR/ARC/AD phenotypes. The level of total serum IgE&gt;1001 IU/ml is a significant predictor biomarker for the developmental risk of AD phenotype combined with AR/ARC/BA in comparison to the AD isolated phenotype in children. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of all participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interest was declared by the authors. Key words: atopic dermatitis, children, phenotype, filaggrin gene, single(nucleotide polymorphism, total immune globulin E.

Autoimmune profile of rat blood in experimental ulcerative colitis

Worldwide incidence of digestive system disorders doubles each decade, thus representing a significant medical and social problem. Despite lacking knowledge in pathogenesis of inflammatory bowel diseases, it is clear that serum cytokine imbalance, and lesions in the walls of gastrointestinal tract are observed in experimental colitis. Pathogenesis of UC remains controversial due to a large set of etiological factors that initiate activation of cellular and humoral mechanisms of the immune response upon development of inflammatory changes in the large intestine. At the same time, cytokine secretion and expression have not been studied in details. The aim of the work was to study the cytokine profile of blood in rats using the experimental oxazolone model of ulcerative colitis. The work was performed in 40 white Wistar rats; ulcerative colitis was induced by rectal administration of a 3% alcohol solution of oxazolone. For anesthesia, Zoletil-100 (INN: tiletamine hydrochloride, VirbacSanteAnimale; France) was used at a dose of 20 mg/kg. The studies were carried out on the 2nd, 4th and 6th days. Serum concentration of IL-6, IL-8, IL-17 and IL-23 was determined by means of automatic ELISA analyzer “Personal LAB” using a specific test system for rats. For immunohistochemistry of Treg cells, we used anti-FoxP3 antibody (Arigo Biolaboratories, Тайвань) followed by immunhistostaining in VENTANA BenchMark XT (USA). Statistical evaluation was performed by non-parametric Mann-Whitney and Wald-Wolfowitz criteria. The difference was considered significant at р ≤ 0.05. In rats with experimental colitis, an increase of proinflammatory IL-17 which acts by attraction of neutrophils and other cells of innate immunity, supporting chronic inflammation and autoimmune reactions. We have found an increase of serum IL-23 concentration in rats with experimental ulcerative colitis on days 2, 4 and 6 of the experiment. This cytokine induces and maintains the inflammatory process in the wall of the large intestine. Significant decrease of FoxP3+Т-lymphocytes was revealed in colonic tissues, thus suggesting appropriate local autoimmune disorders.

ETHNOBOTANICAL STUDY OF DIGESTIVE SYSTEMS DISORDERS IN BADUY ETHNIC, INDONESIA

Digestive disorders rank among the most common problems faced in Indonesia, especially for the Baduy people in Banten Province. Although the Baduy live in water-rich areas, their lack of sanitation facilities and unawareness of methods of disease prevention have prompted high morbidity and mortality rates in their communities, largely due to digestive system disorders that they continue to treat with medicinal plants. This survey was undertaken to document Baduy indigenous medicinal plants that were used to treat and prevent different types of digestive system disorders in their communities using quantitative ethnobotanical approaches. Ethno medicinal data were collected from 30 informants regarding their knowledge on medicinal plants. Quantitative approaches were used to determine the use value and informant consensus factor values of collected data. The results revealed that the Baduy currently use 54 medicinal plant species belonging to 30 families to treat digestive system disorders. Additional research is required, however, to validate the function of the medicinal plants and identify their active compounds.