Different Regions Of Poland Research Articles

Follow-up on the diagnostic level of children covered by the Universal Neonatal Hearing Screening Program in Poland, divided into voivodships.

Thanks to the Polish Universal Neonatal Hearing Screening Program (PUNHSP), all newborns in Poland undergo a free, screening hearing examination. Between 2006 and 2015, the average number of tested children per year was 373,477. According to the analysis of The Central Database (CDB), only 55.8% of the children attended the detailed hearing examinations at the second level of the Program. The aim of this study is to analyse the dates concerning the attendance of the children at the diagnostic level of PUNHSP in different regions of Poland. To conduct an analysis of this fact and find out the reasons for low attendance at the second level in 2015, a telephone survey questionnaire was developed for parents who had not registered their babies for further consultation - 3,239 randomly selected parents. The analysis revealed that the number of children examined at the second diagnostic level of the program is in fact much higher than the results of The Central Database show. The actual number is 83.6% as opposed to 55.8%. As a result of the telephone questionnaire some inaccuracies in the input data to the CDB were detected. The main errors in gathering the information for the CDB were incorrect OAE test result and no examination performed. C onclusion: In Poland the worst results (i.e. questionnaire results compared to CDB) for the attendance at the diagnostic level were shown in Pomorskie, Lubelskie, Mazowieckie and Podlaskie regions. In many cases there was a large discrepancy between the reality and the information in the CDB. The improvement of clarity concerning the CDB application is important in order to minimise the possibility of malformation in the CDB.

The Anti-Staphylococcal Potential of Ethanolic Polish Propolis Extracts.

The principal objective of this study was to determine the anti-staphylococcal potential of ethanol extracts of propolis (EEPs). A total of 20 samples of propolis collected from apiaries located in different regions of Poland were used in the study. The two-fold broth microdilution method revealed some important differences in the antimicrobial activity of investigated EEPs. Up to the concentration of 4096 µg/mL no activity was observed against Gram-negative bacteria (E. coli and P. aeruginosa). Staphylococci exhibited much higher susceptibility. The highest efficiency observed for EEP12 and EEP20 (MIC values ranged between 32 and 256 µg/mL). However, the achievement of bactericidal effect usually required higher concentrations. In the case of clinical isolates of S. aureus MBC values for EEP12 and EEP20 ranged from 512 to 1024 µg/mL. The HPLC analysis revealed that these two products contained a higher concentration of flavonoids (flavonols, flavones, and flavanones) compared to other investigated EEPs. In checkerboard test, a synergistic anti-staphylococcal effect was observed for the action of EEP20 in combination with amikacin, kanamycin, gentamycin, tetracycline, and fusidic acid (all these antibiotics inhibit protein synthesis). Moreover, the investigated EEPs effectively eradicated staphylococcal biofilm. The obtained results clearly confirm the high anti-staphylococcal potential of propolis harvested in Polish apiaries.

Increased Employment Rates and the Nature of the Economic Growth in Poland’s Voivodships

The purpose of the article is to study the impact of basic production factors (level of employment and value of fixed assets) on the economic growth rate in different regions of Poland. The hypothesis presented in the article is that in the process of economic growth, the human factor (level of employment) plays a greater role than the fixed assets. The hypothesis was verified using the data published by the Central Statistical Office in the Statistical Yearbooks of the Regions for the years 2000 and 2015. The results of the study confirm the validity of the formulated hypothesis. Also, the author of the article made the effort to identify the voivodships characterized by extensive and intensive economic growth.

Water Needs of the Ash-Leaved Maple (Acer negundo L.) at the Period Over Three Years after Reclamation in Different Regions of Poland

Water Needs of the Ash-Leaved Maple (Acer negundo L.) at the Period Over Three Years after Reclamation in Different Regions of Poland

Elemental composition of selected species of mushrooms based on a chemometric evaluation

The aim of the study was to determine 16 elements by FAAS and ICP-AES in ca. 1500 samples of 22 species of mushrooms collected from different regions of Poland and the area around Umeå in Sweden. Chemometric techniques were applied to differentiate samples with respect to their geographical origin and interspecies differentiation. Samples of Cantharellus cibarius (Fr.), Boletus edulis (Bull.) and Leccinum scabrum (Bull.) Gray from Morąg, Augustów, the Zaborski Landscape Park, Tarnobrzeg and Umeå were discriminated by factor 1 and factor 2. Some species, i.e. Cantharellus cibarius, Boletus edulis, Boletus pinophilus (Pilát & Dermek), Leccinum aurantiacum (Bull.) Gray, Leccinum scabrum and Leccinum versipelle (Fr. & Hök) Snell from one region of Poland (Augustów or Morąg) were discriminated by K, Na, Mg, Ca, Fe, Zn, Cu, Mn and Cd. The results enabled an assessment of the hypothetical percentage realisation of the recommended dietary intake (RDA) for the bio-elements in question and of provisional tolerable weekly intakes (PTWI) of toxic metals from the consumption of 100 g of mushrooms. The most abundant element in all the mushroom samples was K, especially in Gomphidius glutinosus (Schaeff. ex Fr.) (Umeå - Sweden) and Cantharellus cibarius (Poland - Morąg). Lycoperdon perlatum (Pers.) from Poland and Sweden tended to accumulate the highest levels of Mg, Fe, Zn and Cu. The highest percentage of RDA was obtained for K, Mg and Cu. Based on the estimated PTWI, it can be concluded that no health hazard is associated with the consumption of these mushrooms.

Ambrosia pollen season in selected cities in Poland in 2018

Ambrosia causes most pollen allergies in North America. After several Ambrosia species were introduced to Europe, an increase in the incidence of allergy to pollen of these plants has been observed in many countries. The aim of this study was to compare Ambrosia pollen seasons in 2018 in 13 cities located in different regions of Poland: Bialystok, Bydgoszcz, Cracow, Drawsko Pomorskie, Lublin, Olsztyn, Opole, Piotrkow Trybunalski, Sosnowiec, Szczecin, Warsaw, Wroclaw and Zielona Gora. The study was conducted by the volumetric method using Burkard or Lanzoni pollen samplers. The pollen season was determined by the 98% method. The earliest pollen season start dates (the end of July) were recorded in Zielona Gora, Bydgoszcz, Opole and Szczecin, while the latest ones in Drawsko Pomorskie and Bialystok. The longest pollen seasons occurred in Opole, Szczecin and Zielona Gora (79 days). The highest average daily concentrations of Ambrosia pollen were recorded in Bialystok (129 P/m3) and Lublin (99 P/m3), while the lowest ones in Drawsko Pomorskie and Szczecin (4 and 10 P/m3, respectively). The annual pollen sum reached the highest value in Opole (567 pollen grains) and Zielona Gora (555 pollen grains). It can be concluded from the pattern of Ambrosia pollen seasons at the monitoring sites studied that pollen of this taxon originates not only from Ambrosia locations in Poland but also from long-distance transport.

Occurrence of ergot and its alkaloids in winter rye harvested in Poland

Ergot (Claviceps purpurea) is a fungal pathogen that infects various grass and small grain cereal species, most often open-pollinated grasses, including rye and triticale. We tested 122 samples of rye grains harvested in three different regions of Poland in 2016 and 2017 for ergot and its alkaloids. Ergot sclerotia were found in all samples. The mean content of ergot sclerotia in grain ranged between 0.74 and 1.06 g/kg, and the mean concentration of ergot alkaloids in grain ranged between 270.1 and 580. 4 μg/kg, depending on the region of cultivation. 37% of the samples were infected with ergot below the 0.5 g/kg level set by the European Commission as the maximum permissible level for ergot, and in those samples the mean ergot alkaloids concentration was 155.8 μg/kg (range 4.7-667.9 μg/kg). A statistically significant correlation (R2=0.6941) between ergot content and concentration of ergot alkaloids was found. Ergot alkaloids concentration in grain was re-calculated into ergot alkaloids concentration in sclerotia, and was found to vary widely from 114 to 3,167 mg/kg. Ergot alkaloids profiles were most frequently dominated by R-configured ergopeptides, such as ergocryptine, ergocornine and ergocristine (31, 29 and 16% of the samples, respectively).

The frequency of occurrence of pathogenic and saprotrophic fungi in pea seeds in different regions of Poland

Field pea seeds (cv. Tarchalska) grown in different regions of Poland (Warmia and Mazury, Wielkopolska, Kujawy) were subjected to mycological analyses. Simpson’s dominance index (DI) was used in the study to determine the abundance of each fungal species in the fungal communities isolated from seeds. PCR analyses were carried out with the use of species-specific primers for Fusarium fungi isolated from pea seeds to identify Tri genes responsible for trichothecene synthesis in the growing seasons of 2011, 2012 and 2013.Saprotrophic species predominated on pea seeds cv. Tarchalska grown in various Polish regions, whereas the isolated fungal pathogens were represented by species of the genus Fusarium and Botrytis cinerea. Genes encoding the synthesis of trichothecenes, the mycotoxins produced by Fusarium fungi, were not identified in the PCR analysis. In the group of peas grown in three Polish regions (Warmia and Mazury, Wielkopolska, Kujawy), the highest level of fungal colonization was noted in the pea seeds harvested in Warmia and Mazury.

The diet structure and body mass index among Polish preschool children in relation to their place of residence.

Introduction and aimProper nutritional status in early childhood makes it possible for children to achieve their genetically-determined growth potential and intelligence. A child’s nutritional status is due to economic, cultural, psychosocial and geographic factors. The present study aims to check whether the nutritional status of preschoolers differs depending on their place of residence.Materials and methodsIn order to achieve this goal we used 24-h dietary recalls to assess the children’s diet based on interviews with 530 mothers of preschool children from five different regions of Poland. Moreover, the children’s BMIs were calculated.ResultsWe found differences in the level of anthropometric indicators, diet structure and what percentage of the estimated average requirement for energy was met depending on the region where the children live. Youngsters from the western region of Poland had significantly higher BMIs than those from other regions. What is more, the percentage of meeting the estimated average requirement for energy and the percentage of calories from carbohydrates was higher among children from the western region, as compared with preschoolers from other areas of the country. The amount of fats and protein in the diet of children from western Poland, in turn, was significantly lower as compared with the diet of their peers from other regions.ConclusionsThe present study provides evidence that the nutritional status of children should be analyzed in the context of their place of residence. Thus, the differences between regions and their impact on the health status of their inhabitants should be taken into account when designing preventive or corrective measures.

Herd-level seroprevalence of Fasciola hepatica and Ostertagia ostertagi infection in dairy cattle population in the central and northeastern Poland

BackgroundFasciola hepatica and Ostertagia ostertagi infections are widespread in cattle population of Europe, however data on their prevalence in Poland are only fragmentary. Therefore, the cross-sectional study was carried out to determine the herd-level seroprevalence of F. hepatica and O. ostertagi infection in dairy cattle population in the central and north-eastern provinces Poland, and to identify basic local risk factors for these infections. In total, 598 herds were enrolled, 394 (65.9%) in the north-eastern province and 204 (34.1%) in the central province. In each herd the questionnaire survey was conducted and bulk-tank milk (BTM) sample was collected and screened using two indirect immunoenzymatic tests. Optical density ratio (ODR) was regarded as the quantitative proxy of exposure to either of the two parasites.ResultsBoth Fasciola and Ostertagia ELISA ODR in the north-eastern province was significantly higher than ODR in the central province. At the cut-off value of ODR = 0.27 the true herd-level seroprevalence of F. hepatica was 79.6% (95% CI: 74.0%, 84.3%) in the north-eastern province and 13.0% (95% CI: 5.3%, 21.7%) in the central province. At the cut-off of ODR = 0.50151 of 188 herds (80.3%, 95% CI: 74.1%, 85.4%) were seropositive for O. ostertagi in the north-eastern province and only 70 of 136 herds (51.5%, 95% CI: 43.1%, 59.7%) were seropositive in the central province. Location of a herd in the north-eastern province, longer grazing period practiced in a herd and > 50%-share of grazing grass in roughage were all positively related to the increase in exposure to both parasites. Moreover, the use of hay or haylage as main roughage proved to be positively related to the increase in exposure to F. hepatica.ConclusionsF. hepatica and O. ostertagi are widespread in cattle population in Poland, however their occurrence at a herd-level varies between different regions of Poland. This diversity can only partly be explained by different herd management, and appears linked to environmental and climate conditions typical for these regions.

An algorithm for selecting groups of factors for prioritization of land consolidation in rural areas

Large defects in the spatial structure of agricultural land are an issue in many countries. The main cause of these defects are hundreds of years of social, economic and historical transformations. The spatial structure of farmland can be improved using procedures such as land consolidation and exchange. However, given the fact that the funds for these activities are limited, it is necessary to select those areas in which land consolidation and exchange are a priority. To develop a prioritization plan for land consolidation and exchange interventions, it is necessary to carry out a series of studies and analyses which will provide a set of factors characterizing the region under investigation. Many years of research have shown that different regions in Poland are characterized by different parameters.Therefore, in the present study, an attempt was made to develop a universal algorithm for selecting groups of factors for purposes of prioritization of land consolidation which takes into account the geographic location of an investigated area. To test the proposed algorithm, analyses were carried out in two communes located in central and south-eastern Poland which differed significantly in terms of the spatial structure of farm holdings. A first test object was the commune of Paradyż, occupying an area of 8125.73 ha and divided into 15,941 cadastral plots. A second test object was the commune of Frysztak, located in south-eastern Poland, with an area of 9066.86 ha, divided into 21,506 cadastral plots.

Recycling and segregation of used aluminium beverage cans according to the residents of Silesia voivodship

Abstract Aluminium is one of raw materials that can be practically continuously recycled. Thanks to the proper sorting of aluminium scrap it is possible to produce precisely the same products that it was made before Selective waste collection and an extensive network of waste collection points have significant impact on the recovery level of used aluminium beverage cans. The purpose of the article was to analyse the results of surveys on aluminium segregation in Silesia voivodship. According to literature people are increasingly interested in environmental protection and what is happening with waste generated by them. But there is lack of information about people from different regions of Poland. From the research presented in the paper it can be concluded that people realize that used aluminium beverage cans become packaging waste which can be easily recycled so most of them segregate them in everyday life. This is the result of changes in the legislation on municipal waste and their segregation, as well as the element of environmental education.

Development and validation of multi-residue analysis for tetracycline antibiotics in feed by high performance liquid chromatography coupled to mass spectrometry

ABSTRACTA new multi-residue method for the analysis of the tetracycline antibiotics oxytetracycline, tetracycline, chlortetracycline, and doxycycline was developed and validated for animal feed. After extraction with 0.1M Na2EDTA-McIlvaine buffer (pH 4), the samples were centrifuged, purified by SPE (Strata-X-CW cartridges) and analysed by HPLC-MS. Validation of the method was performed according to the guidelines indicated in European Commission Decision 2002/657/EC. The procedure was validated by spiking feed samples at three different levels (300, 1000, and 5000 µg/kg). Average recoveries for tetracyclines were in the range 79.70–98.8%, with RSD for repeatability and reproducibility in the ranges of 5.0–9.5% and 6.5–11.0%, respectively. The method was successfully validated and proved to be efficient, precise, and useful for quantification of tetracyclines in animal feed. The validated method was successfully applied to 65 suspect feed samples collected from different regions of Poland in 2015–16 and obtained from farms and feed manufactures. Of these 65 purportedly non-medicated feeds, eight (12.3%) were positive for the presence of doxycycline or chlortetracycline.

Isolation and phenotypic and morphological characterization of the first Podoviridae lytic bacteriophages \u03d5A38 and \u03d5A41 infecting Pectobacterium parmentieri (former Pectobacterium wasabiae)

Two bacteriophages, ϕA38 and ϕA41, infecting Pectobacterium parmentieri strain SCC 3193 (former Pectobacterium wasabiae strain SCC 3193) were isolated from arable soil samples collected in different regions of Poland. ϕA38 and ϕA41 have a typical morphology of the members of the family Podoviride and order Caudovirales, with a head diameter of ca. 60 nm and tail length of ca. 20 nm. Phages ϕA38 and ϕA41 exhibited a similar RFLP pattern with Csp6I restriction endonuclease. They were stable in a range of pHs, temperatures and osmolarities but were rapidly inactivated by UV light. During the first 20 min., 74 and 69% of ϕA38 and ϕA41 phages, respectively, were adsorbed to SCC 3193 cells. In one-step growth experiments, ϕA38 and ϕA41 showed latent period of ca. 20–30 min and burst size of 102 and 141 phages, respectively. The optimal multiplicity of infection (MOI) was calculated to be 0.01 for both bacteriophages. In the host range experiments, both phages were able to infect six from 21 of the tested P. parmentieri isolates but the phages were unable to infect other members of the Pectobacterium spp. or Dickeya spp. In the proof-of-concept experiments, ϕA38 and ϕA41 were able to inhibit the growth of P. parmentieri strain SCC 3193 and to protect potato tuber tissue maceration caused by the bacterium. The potential use of ϕA38 and ϕA41 bacteriophages for the biocontrol of P. parmentieri in potato is discussed.

Fungal colonization of seeds of three lupine species in different regions of Poland

The health status of seeds of three lupine species (white lupine, narrow-leaved lupine, and yellow lupine) from different regions of Poland was investigated. Seeds were analyzed by microscopic method and PCR. The examined lupine seeds were colonized by saprotrophic fungi of the genera <em>Alternaria</em>, <em>Cladosporium</em>, and <em>Penicillium</em>, and pathogenic fungi of the genera <em>Fusarium</em>, <em>Botrytis</em>, <em>Mycosphaerella</em>, and <em>Colletotrichum</em>. The relative frequency (RF) of fungi detected on lupine seeds from the regions of Kujawy, Wielkopolska, Lower Silesia, and Warmia and Mazury was determined. The highest RF values of pathogenic fungi were noted in Lower Silesia in 2012 and 2013, and in Warmia and Mazury in 2011. The RF values of pathogenic and saprotrophic fungi on lupine seeds harvested in different regions of Poland were affected by weather conditions. PCR analyses revealed the presence of <em>Tri</em> genes in the seeds of narrow-leaved lupine. The analyzed seeds were relatively free of pathogenic fungi and could be used for sowing and feed production.

AVAILABILITY OF LAND RESOURCES IN INDIVIDUAL FARMS IN POLAND AND CONVERGENCE OF THE FARM’S INCOME AFTER POLAND’S ACCESSION TO THE EU

The aim of the article is to determine whether, after the Polish accession to the EU, there were trends for convergence/divergence income of individual farms from areas with different or similar equipment in the land factor (quantitative and qualitative). The study included individual farms FADN in different regions in Poland. The analysis were based on data from the statistical publications of the Central Statistical Office of Poland as well as accounting information on income from agricultural activity in individual farms covered by the Polish FADN. It has been proven that the equipment in the land factor is the determinant of leveling the value of agricultural income (convergence) between farms from areas with similar equipment in this factor.

An outbreak of leptospirosis imported from Germany to Poland.

Leptospirosis is a zoonotic disease caused by spirochetes of the Leptospiraceae family. In both humans and animals the main route of infection is indirect contact - through water or other products contaminated with urine containing spirochetes. Infection most commonly occurs through ingestion of water or food contaminated with Leptospira spp. The aim of the study was to characterize cases of leptospirosis imported to Poland from Germany in 2014 and to analyze methods that are helpful for making a diagnosis. The 10 patients examined were reported as suspected leptospirosis cases on the basis of clinical symptoms and epidemiological investigations. They originated from different regions of Poland and had been working together at a strawberry plantation in the Cloppenburg district of Lower Saxony in Germany. Blood and urine samples were tested by PCR and serum samples by serology. All ELISA positive and negative cases were examined using a reference microscopic agglutination test (MAT). In the tested group, 6 individuals (60%) were seropositive according to the ELISA, and 2 of them were confirmed by the MAT. The PCR results for the blood and urine samples were negative. Using the ELISA in the diagnosis of leptospirosis allowed the disease to be identified much faster, differentiating classes of antibodies and recognizing levels of them that are too low to be detectable by the MAT.

Selected Effects of Financing Agriculture from a Regional Perspective

AbstractThis paper contains an assessment of changes in the financial and property situation of agricultural holdings in different regions of Poland. In the years 2006-2013, growth of the level of income from a family-owned farm was registered in all regions, but capabilities of reproducing fixed assets and development capabilities varied. Favourable changes in the availability of fixed assets in farms were observed. Regional differences became apparent in the efficiency with which these assets were used. The greatest growth of the value of fixed assets per 1 ha of farmland was registered in the Pomorze and Mazury region. Farms in the Małopolska and Pogórze region were in the most difficult situation, characterized by a permanent inability to reproduce fixed assets and develop.

First detection of porcine circovirus type 3 on commercial pig farms in Poland.

Porcine circovirus type 3 (PCV3) is a novel circovirus species recently discovered in USA and China in cases of porcine dermatitis and nephropathy syndrome, reproductive failure, respiratory disease and multisystemic inflammation. This study reports on the first identification of PCV3 in Europe, in serum from pigs from Polish farms. A total of 1,050 serum samples were collected between 2014 and 2017 from sows and 3-20weeks old pigs from 14 commercial farms representing different regions of Poland, different size and health status. The samples were pooled by 4-6 and tested with real-time PCR for PCV3. PCV3 DNA was detected in 12 of 14 farms (85.7%). On the PCV3-positive farms, the virus was detected in 5.9% to 65% serum pools. PCV3 was most common among weaned pigs and finishers (26.1% and 28.0% of serum pools, respectively). Sequence analysis of 359 nucleotide fragment of ORF2 showed highest identity of 99.7% to PCV3-US/SD2016 from USA. Our results indicate that PCV3 is a common virus among Polish pigs but no links to unexplained disease conditions were established.

Hydrogen peroxide-dependent antibacterial action of Melilotus albus honey.

Melilot honey is used in traditional medicine as an anticoagulant agent due to the possibility of the presence of the coumarin compounds which are specific for Melilotus plant. Melilotus albus is rarely used to produce honey, and antibacterial properties of this variety of honey had not been studied yet. Nine samples of melilot honey produced in different regions of Poland were analysed according to their antibacterial activity which was correlated with physiochemical parameters and antioxidant activity. It was shown that antibacterial activity of melilot honey is created by hydrogen peroxide and other bioactive compounds.