Effect Of Diet Research Articles

Intervention effects of multilayer core-shell particles on colitis amelioration mechanisms of capsaicin.

The intervention effects of delivery systems on the digestion and adsorption profiles and, thus, the pharmacological effects of bioactive compounds represent an intriguing scientific hypothesis that can be proven with research case studies. Delivery systems with tailor-made structures fabricating from the same building materials offer a new research strategy for deciphering the modulating effects of the digestive fate on the therapeutic efficacy of encapsulated bioactive compounds. Herein, we developed capsaicin-loaded core-shell nanoparticles (Cap NPs), microparticles (Cap MPs) and nano-in-micro particles (Cap NPs in MPs) and investigated their regulatory effects on the digestive fate and colitis-alleviating mechanisms of capsaicin. Results suggested that the small intestine dominant absorption of Cap NPs differed significantly with the colorectal dominated accumulation of Cap MPs and Cap NPs in MPs in terms of the colitis alleviating mechanisms. Cap NPs alleviated colitis mainly through promoting the colonization of short-chain fatty acid-producing bacteria, maintaining intestinal barrier homeostasis and partially inhibiting the activation of the NF-κB pro-inflammatory pathway. Whereas, better dietary intervention effects were achieved from Cap NPs in MPs via promoting the proliferation of mucus-related bacteria and enhanced triggering efficiency on the TRPV1-mucus-microbiotas cyclic cascade. This work confirmed that rationally designed biomaterial-based delivery vehicles can flexibly interfere with the therapeutic mechanisms of encapsulated cargos, representing a new horizon in the field of precise nutrition.

Dietary effects of sophorolipids on nutrient bioavailability and intestinal microenvironments in broiler chickens

Using antibiotics as growth promoter has been banned in poultry feed industry, thus various researchers try to seek an alternative to replace the growth-promoting antibiotics. In this study, we aimed to evaluate the growth performance via intestinal nutrient utilization and cecal microbial composition of broiler after dietary supplementation with most commonly using antibiotics, zinc bacitracin, and sophorolipid. A total of 180 1-day-old chicks were randomly assigned, and dietary treatment was as follow: CON, basal diet; ZB, 100 ppm of zinc bacitracin supplemented diet; and SPL, 250 ppm of sophorolipid supplemented diet. Their growth performance was evaluated and the samples of blood, small intestine, and ileal and cecal digesta were collected for biochemical, histological, and genomic analyses. The body weight and average daily gain of 7-day-old chicks were higher in ZB and those in overall experimental period were improved by ZB and SPL supplementation (p < 0.05). Their intestinal characteristics were not affected by dietary treatments in duodenum and ileum. Nonetheless, villus height was increased by SPL supplementation in jejunum (p < 0.05). Moreover, dietary SPL supplementation could down-regulate the expression level of pro-inflammatory cytokine, IL-1β (p < 0.05). mRNA levels of lipid and protein transporters did not differ among the treatments, however, relative expression levels of carbohydrate transporters, GLUT2 and SGLT1 were increased in broiler chicken's jejumum fed zinc bacitracin and sophorolipid supplemented diets (p < 0.05). Dietary zinc bacitracin supplementation could increase the population of Firmicutes in phylum level, and the portion of Turiciacter in genus level. On the other hands, the portion of Faecalibacterium was increased by dietary SPL supplementation compared to the other treatments. Our findings suggest that SPL supplementation improves growth performance through enhanced carbohydrate utilization capacity via improvement of gut morphological status and modulation of the cecal microbial population of broilers.

Low-FODMAP Diet for the Management of Irritable Bowel Syndrome in Remission of IBD.

Approximately 30% of patients with quiescent inflammatory bowel disease (IBD) meet the diagnostic criteria for irritable bowel syndrome (IBS). The aim of this study was to evaluate the effectiveness of a low-FODMAP diet in patients who meet the diagnostic criteria for IBS whilst in IBD remission. A total of 200 patients in remission of IBD were included in the study. Sixty-five of these patients (32.5%) were diagnosed with IBS according to the R4DQ. On the patients who met the IBS diagnostic criteria, anthropometric measurements, laboratory tests and lactulose hydrogen breath tests were performed. A low-FODMAP diet was introduced for 6 weeks. Of the 59 patients with IBS diagnosed at baseline for whom data were collected at the end of follow-up, after the low-FODMAP intervention IBS-like symptoms were not present in 66.1% (n = 39) (95% CI (53.4%; 76.9%)). The difference between the two groups (with SIBO at baseline (33 of 48 patients) and without SIBO at baseline (6 of 11 patients)) in the low-FODMAP diet's effectiveness was not statistically significant (p = 0.586). The low-FODMAP diet improved the gut symptoms of flatulence and diarrhea. It had no effect on the occurrence of constipation. In IBD patients in remission who meet the IBS criteria, the dietary intervention of a low-FODMAP diet is effective for a reduction in IBS-like symptoms, regardless of the coexistence of bacterial overgrowth.

Lesions of the biliary system in Atlantic salmon (Salmo salar L.).

Lesions of the biliary system in Atlantic salmon (Salmo salar L.).

Effect of feeding wormwood herb (Artemisia absinthium Linn.) added total mixed ration on wool production and quality in sheep

ABSTRACT Emphasis over organic livestock production has resulted in increased interest in natural products. This study aims to evaluate dietary incorporation effect of wormwood (Artemisia absinthium) herb on wool characteristics and economics in sheep. In vitro assay was conducted to determine effective inclusion levels of feed additives followed by in vivo feeding trial for three months on crossbred (15) sheep distributed into three groups: control (Con) fed basal diet, groups fed diet added with 4.50% herb either alone (Hrb) and with 0.60% exogenous fibrolytic enzymes (EFE) (HrbEz), the levels chosen on basis of the best results of in vitro assay. Toward end of feeding trial, six days digestibility trial was conducted after which animals were shorn. Wormwood herb inclusions had significant (P < .05) positive effect on digestible nutrient contents and their intakes. Greasy and clean wool yields were higher (P < .05) resulted in lowering cost of wool production to the tune of 11.3% in Hrb and 22.2% in HrbEz group compared to control with no difference for all fiber quality characteristics except fiber length. It was concluded that incorporation of 4.50% wormwood herb with 0.60% of EFE in diet of sheep resulted in better wool growth economically with no adverse effect on quality.

Does diet influence ejaculate expenditure under experimentally altered risk of sperm competition in guppies?

According to the sperm competition risk (SCR) model, males should increase their investment in ejaculates when there is a high chance that their sperm will compete for fertilization with those from another male. However, as ejaculates are energetically costly to produce, the extent to which males can adjust ejaculate investment may depend on the availability of metabolic resources. We tested these ideas using the guppy, Poecilia reticulata, a livebearing fish known to exhibit strong condition dependence in ejaculate traits and among the highest recorded polyandry rates in any vertebrate. Specifically, we manipulated SCR by visually exposing mature males to females either in the presence (high SCR) or absence (low SCR) of a rival male over 1 month, which comprises a full spermatogenic cycle. To test for condition dependence in male response to SCR, both groups were further subdivided into two diet quantity treatments (high and low), enabling us to test fixed and interactive effects of SCR and diet treatment on ejaculate expenditure (sperm production, sperm length and sperm velocity). Contrary to our predictions, we found no evidence that males increase ejaculate expenditure under high SCR, and indeed report the opposite pattern (higher expenditure under low SCR) for sperm velocity. Furthermore, we found no evidence that dietary restriction (when administered to mature adult males over 1 month) affected any ejaculate traits, leading us to suspect that ejaculates may only be susceptible to dietary restriction effects during a critical ontogenetic stage of development. We discuss these unexpected findings in the context of guppy biology, which may render the species less likely to exhibit long-term adjustments in ejaculates when perceiving short-term changes in SCR. Moreover, the high polyandry rates in this species may mean that the visual presence of a rival is a cue of altered sperm competition intensity, rather than risk.

Dietary dragonhead effects on growth, immunity and antioxidant and related genes expression in zebrafish (Danio rerio)

The present study aimed to investigate the effects of the herbaceous plant, Dracocephalum kotschyi, on the immune response, antioxidant enzymes activity, growth performance, and related genes expression in zebrafish (Danio rerio). An in vivo trial was carried out by feeding zebrafish (initial weight of 0.47 ± 0.04 g) with different levels of powdered D. kotschyi: 0% (C-0%, control), 1% (Dk-1%), 2% (Dk-2%) and 3% (Dk-3%) for 10 weeks. The results show that total protein (TP) and total immunoglobulin (IG), were significantly increased in both skin mucus and whole body extract (WBE). Lysozyme (LYS) activity significantly increased in the WBE of zebrafish fed with D. kotschyi integrated diets compared to the control (P < 0.05). The superoxide dismutase (SOD) of WBE was significantly (P < 0.05) enhanced by dietary D. kotschyi. Additionally, this herb brought about a significant up-regulation in immune (LYS), pro-inflammatory (IL-1β) from intestine, and antioxidant (SOD and CAT) genes in liver, compared to the non-supplemented group (P < 0.05). Growth hormone (GH) and insulin growth factor (IGF) genes expression were upregulated significantly in zebrafish by the addition of D. kotschyi, although body weight did not change. The best results were obtained with the 3% inclusion of powdered D. kotschyi, except for LYS and IGF expressions that were best upregulated by 2% and 3% of D. kotschyi. Our results indicate that powdered D. kotschyi (suggested dose of 3%) could potentially improve zebrafish's immune response and antioxidant defense.

Dietary Interventions in Cancer Treatment and Response: A Comprehensive Review.

Simple SummaryChemotherapy and radiotherapy are essential components to the management of most solid malignancies. These modalities exert their effects primarily by mediating the DNA damage of malignant cells; however, healthy cells are also damaged by the same mechanisms and can incur acute and late side effects resulting in both morbidity and mortality. Dietary interventions have been shown to reduce cancer growth, progression, and metastasis in many different solid tumor models and they show promise for improving cancer outcomes in early phase clinical studies. Here, we review preclinical and clinical studies that examine how dietary interventions can impact cancer treatment toxicity and efficacy in patients who were undergoing chemotherapy and/or radiotherapy. This information can help clinicians tailor the dietary regimens to patients based on their treatment methods and promote larger clinical trials to test the dietary effects on cancer treatment safety and efficacy.Chemotherapy and radiotherapy are first-line treatments in the management of advanced solid tumors. Whereas these treatments are directed at eliminating cancer cells, they cause significant adverse effects that can be detrimental to a patient’s quality of life and even life-threatening. Diet is a modifiable risk factor that has been shown to affect cancer risk, recurrence, and treatment toxicity, but little information is known how diet interacts with cancer treatment modalities. Although dietary interventions, such as intermittent fasting and ketogenic diets, have shown promise in pre-clinical studies by reducing the toxicity and increasing the efficacy of chemotherapeutics, there remains a limited number of clinical studies in this space. This review surveys the impact of dietary interventions (caloric restriction, intermittent and short-term fasting, and ketogenic diet) on cancer treatment outcomes in both pre-clinical and clinical studies. Early studies support a complementary role for these dietary interventions in improving patient quality of life across multiple cancer types by reducing toxicity and perhaps a benefit in treatment efficacy. Larger, phase III, randomized clinical trials are ultimately necessary to evaluate the efficacy of these dietary interventions in improving oncologic or quality of life outcomes for patients that are undergoing chemotherapy or radiotherapy.

The Influence of β-Alanine Supplementation on Recovery Biomarkers in Adults: A Systematic Review and Meta-Analysis

Background: Clinical studies, investigating the effect of β-Alanine (BA) supplementation on recovery biomarkers in physically active individuals, have generated inconsistent results. This systematic review and meta-analysis study aimed to clarify the clinically relevant dietary effects of BA supplementation. Methods: A comprehensive search was done in the electronic databases of Scopus, PubMed, ISI Web of Science and Embase from inception to 2022. Meta-analysis was done using the random-effects model. Pooled effect size was evaluated using standard mean difference (SMD) and 95% confidence intervals (CI). Heterogeneity of between-study was evaluated according to Cochran’s Q test and I2 . Subgroup analysis was conducted to identify the potential sources of heterogeneity. Results: Overall, 32 studies were included in the current study. The results suggested that BA supplementation increases carnosine level significantly (SMD: 0.22mmol/L, 95%CI: -0.17, 0.61, P=0.27) but no effect was shown about lactate, fatigue, VO2 , pH and bicarbonate (HCO3 - ) (P&gt;0.05). Subgroup analysis revealed a significant association of VO2, carnosine and fatigue with supplementation dosage, gender and duration of administration respectively. Conclusion: BA supplementation emerged its beneficial effects on enhancing carnosine level which highlights its ergogenic effects. In contrast, no significant effects had been shown in term of fatigue delay and blood levels of lactate, HCO3 - , pH, and VO2 value. These results warrant more investigation in a prospective design to clarify the exact mechanism in this way.

Multi-Omics-Based Functional Characterization of Hybrid Fermented Broussonetia papyrifera: A Preliminary Study on Gut Health of Laying Hens

More attention has been paid in recent times to the application of Broussonetia papyrifera (BP) silage in ruminants, owing to its nutritional value. This study aimed to characterize the functionality of fermented BP and preliminarily explore its dietary effects on the gut health of laying hens. In this study, we characterized the antioxidant and antibacterial activities, bioactive compound profile, and bacterial community in Lactobacillus plantarum-fermented BP (FBP), as well as its dietary effects on intestinal morphology, microbiota and gene expression of laying hens. Improved contents of protein, total polyphenol and flavonoids as well as antioxidant and antibacterial activities were found after fermentation of BP. Untargeted metabolomics displayed more abundant apigenin, luteolin, diosmetin, and quercetin within the FBP, which may contribute to its functionality. Microbiome demonstrated increased abundance of Firmicutes at the expense of Cyanobacteria phylum, accompanied with raised levels of Lactobacillus genus. The results of a feeding trial showed dietary FBP supplementation increased the serum superoxide dismutase, but down-regulated gene expression of aryl hydrocarbon receptor (AhR), mucin2, and ZO-2, without obviously affecting the intestinal morphology and colonic microbiota. These findings suggest that FBP warrants further investigation as it may serve as a functional dietary supplement in laying hen feed.

Comportamiento productivo de corderos y corderas alimentadas con un cofactor glucogénico bajo condiciones de estrés por calor

This study aimed to evaluate the dietary effect of the thiamine diphosphate (TD) glycogenic cofactor on the productive performance of male (M) and ewe (E) lambs exposed to outdoor conditions natural conditions of high summer temperatures in an arid region. Twenty 4-month-old Dorper x Katahdin sheep were used, which were assigned to the following treatment combinations (n=5) according to a 22-factorial arrangement under a randomized complete block design: 1) M without TD, 2) M with TD, 3) H without TD and 4) H with TD. No productive performance variable was affected (P>0.05) by the interaction or the DT main effect. M-lambs had higher (P>0.05) growth rate, feed intake and final weight than E-lambs. It is concluded that, regardless of the sex, DT does not improve productive performance of feedlot hair sheep under heat stress.

Dietary protein hydrolysate effects on growth, digestive enzymes activity, and expression of genes related to amino acid transport and metabolism of larval snakehead (Channa argus)

A feeding trial was conducted to investigate the effects of dietary protein hydrolysate on the growth, digestive enzyme activity, and expression of genes related to amino acid transport and metabolism in larval snakehead. Five isonitrogenous (crude protein, 59%) and isolipidic (crude lipid, 13%) diets were formulated using pre-mixed protein hydrolysates to replace approximately 0% (control, PH0), 25% (PH25), 50% (PH50), 75% (PH75) and 100% (PH100) un-hydrolyzed pre-mixed protein. Triplicate groups of snakehead larvae (17 days post-hatching, initial body weight 122 ± 0.10 mg) were randomly assigned to 15 fiberglass fish tanks with a density of 750 larvae per tank, and fed four times daily for 20 days. At the end of the feeding trial, the growth metric was monitored, and tissues, including intestine and muscle, were separated for physiology and molecular biology analysis. The results showed that dietary protein hydrolysate quadratically increased final body weight (FBW, 2.57–3.73 g) and survival rate (SR, 53.33–60.14%), with the maximum value being observed in the PH75 group (FBW, 3.73 g; SR, 60.14%). Meanwhile, the optimal level of dietary protein hydrolysate elevated the activity of digestive enzymes including trypsin (1558.24–2520.37 U/gpro), lipase (22.37–36.26 U/gpro) and alkaline phosphatase (3.05–3.98 U/mgpro). Gene expression results revealed that protein hydrolysate inclusion quadratically elevated the expression of intestinal amino acid transporter y+LAT2 (0.96–1.51) and peptide transporter PepT1 (0.68–1.22), with the maximum value observed in the PH75 (1.51) and PH50 (1.22) groups, respectively. Meanwhile, the expression of muscle PepT1 (0.97–1.73) was up-regulated linearly, and y+LAT2 expression (0.54–1.01) quadratically decreased with the inclusion of protein hydrolysate. Dietary protein hydrolysate appeared activate the target of rapamycin (TOR) pathway in the muscle through the up-regulation of TOR expression (1.00–1.20) and down-regulation of 4EBP1 expression (0.56–1.01). Additionally, the expression of genes involved in the amino acids response (AAR) pathway in muscle, eIF2α (0.50–1.03), CHOP (0.36–1.09) and REDD1 (0.27–0.98), were linearly inhibited with the inclusion of protein hydrolysate. In above, the optimal level of dietary protein hydrolysate (75%) elevated the growth performance and improved the digestive tract of snakehead larvae. The beneficial role of protein hydrolysates in growth and development is partly attributed to the regulation of genes related to amino acid transport and metabolism.

The effects of dietary supplementation of common mugwort (Artemisia vulgaris) meal on growth and excreted Eimeria spp. oocysts incidence in Dwarf Lop rabbit kits

The goal of the study was to evaluate the effect of dietary inclusion of common mugwort (Artemisia vulgaris) herb on selected growth and health traits of young dwarf rabbits. The mugwort species represent regular botanical species with proven favourable effects on health state of many animal species. However, a dietary effect of Artemisia vulgaris (AV) on health of dwarf rabbit genepool has not been studied yet. The study was performed on a total of 12 kits of the Dwarf Lop breed. These kits were divided into 2 groups (control, C; experimental, E). From the age of 21 days up to the age of 112 days the kits in the experimental group received a&nbsp;complete diet specifically designed for the dwarf rabbits with 10% supplementation of the AV meal. There was found no significant adverse effect on live weight and average daily weight gain in rabbits of the E group. Concerning the anticoccidial effect, the 112-day-old rabbits fed the E diet showed a significantly lower (P &lt;0.05) number of excreted oocysts (1,766 OPG) as compared to those in the C group (6,845 OPG). Based on the results of the present study, there can be suggested that 10% dietary inclusion of the AV meal represents a safe share and it showed a positive effect on the excreted coccidiosis count in dwarf rabbits. However, it would be suitable to perform further studies, when the optimization of a mugwort dietary level during specific life periods of dwarf kits and the selection of other Artemisia botanical species will take a place. In addition, monitoring of more growth traits and biological effects of particular substances contained in Artemisia sp. would be studied on dwarf pet rabbits as well.

English

This study was designed to check the dietary effect of different levels of lysine on intestinal morphology, cecal tonsils and goblet cell count in Japanese Quails. For this Purpose, a total of 100, day old chicks were randomly divided in four groups. These groups were further sub-divided into five replicas per group, each replica having five birds. NRC has recommended 13g/kg of lysine in basal diet in 1994. The birds of control group (first) were fed with basal diet containing 13 g/kg of lysine, second experimental groups fed contained lysine at the rate of 11.7g/kg of lysine, third group 13.6 g/kg of lysine and fourth group 14.9 g/kg, respectively for 35 days. The results showed a significant increase (P&lt;0.05) in villus height (VH), villus width (VW), villus surface area (VSA) and muscular mucosa thickness (MMT) in all parts of the small intestine of 13.65 g/kg supplemented birds. In jejunum, VH, VW, VSA, lamina propria thickness (LPT) and MMT was increased at levels 13.65 g/kg and 14.95 g/kg. Crypt depth (CD) and muscularis externa thickness (MET) did not vary in both duodenum and jejunum, in ileum CD and MET was increased (P&lt;0.005) in treated groups 13.65 g/kg and 14.95 g/kg. LPT was increased (P&lt;0.005) in duodenum and jejunum, while in ileum it was insignificant. Total goblet cells count was increased in all duodenum, jejunum and ileum. In duodenum acidic mucin and total GC count was significant (P&lt;0.005) in lysine treated groups at 13.65 g/kg and 11.7 g/kg level while mixed mucin GC count was non-significant. In jejunum and ileum portion acidic mucin, mixed and total GC count was higher (P&lt;0.005) in 13.65 g/kg group. In cecal tonsils length, width, area and number of lymphatic nodule and was significant (P&lt;0.005) in 13.65 g/kg. Lysine supplementation of 13.65 g/kg is beneficial feed additive for Japanese Quails on intestinal morphology

Dietary enrichment with crude protein content and feed additives (Bacillus spp. and yeast strains) improves growth performance, survival and circulating hemocytes in juvenile White shrimp, Litopenaeus vannamei

In this study the enrichment dietary effect with different crude protein levels (CP) and feed additives on growth, survival, feed conversion ratio (FCR), feed consumption (FC) and total hemocyte count (THC) in juvenile white shrimp Litopenaeus vannamei were evaluated. The study covered two bioassays: in the first one, juveniles were daily fed for 45 days with four experimental diets containing: (1) Control, commercial feed (35% CP); (2) 29% CP; (3) 32% CP; (4) 35% CP. After the 29% CP diet was selected, juveniles in bioassay II were daily fed for 45 days with a single CP diet complemented with probiotics: (1) Control, commercial feed (35% CP); (2) 29% CP; (3) 29% CP + Bacillus mix at 1×106 CFUg–1 feed; and (4) 29% CP + yeast mix at 1×106 CFUg–1 feed. Juvenile shrimp fed with experimental diets gained significantly more weight and increased survival, FCR, FC and THC compared with control diet. However, differences among experimental diets were not significant. In bioassay II, juvenile shrimp fed with experimental diet + feed additives significantly increased survival, FCR, FC and THC compared with control diet. Growth of juveniles significantly increased with 29% CP and 29% CP + yeast mix diets, compared with control group. Complementing the diet with yeast mix showed higher survival and THC of juveniles compared with the other experimental treatments. Different CP levels in shrimp diet improved growth, survival and circulating hemocytes, and addition of mixed yeast as feed additive induced better survival and immune response in juvenile shrimp.

Effect of dried orange peel on egg production and quality of laying hen

Utilization of agricultural by-products in animal nutrition is a practice as old as the domestication of animals. The dried orange peel (DOP), by-product of citrus industry is a promising source of diversified bioactive ingredients with beneficial effects on health. Therefore, this study was carried out to assess the dietary effect of DOP on production and, internal and external egg quality characteristics of laying hens. A total of 60 layers of 25 weeks of age were segregated into three dietary treatment groups using a completely randomized design and reared up to 42 weeks. The dietary treatments were control (basal diet), 5% DOP (basal diet + 5% DOP), and 10% DOP (basal diet + 10% DOP). The study revealed that dietary DOP significantly decreased the feed consumption and feed conversion ratio of layers (p &lt; 0.05) without having any significant effects on egg production, egg weight and egg mass. None of the exterior egg quality parameters were significantly affected by the dietary supplementation of DOP (p &gt; 0.05). Among interior egg quality attributes, the albumen height, albumen index, yolk color, and Haugh unit were all considerably elevated through supplementation of 10% DOP (p &lt; 0.05). Nevertheless, the yolk index was raised by both 5% and 10% DOP supplementation (p &lt; 0.05). The relative weight of ovarian grape and oviduct did not affect by the supplementation of DOP with layer diet (p &gt; 0.05). In summary, it may be said that layer diets can be supplemented with DOP up to 10% level to improve FCR, albumen index, yolk index, yolk color and Haugh unit without compromising egg production or exterior egg quality. Further studies are recommended with layer diets supplemented with DOP at levels higher than 10% to determine the ideal DOP dose during other production periods in various layer strains.&#x0D; Bangladesh Journal of Animal Science 51 (3): 98-106, 2022

What happens to Hoplias malabaricus fed on live prey (Astyanax altiparanae) previously exposed to copper? A multiple biomarker approach

Copper waterborne toxicity is well understood in aquatic organisms. However, the dietary copper effects are much less known, especially in tropical fish. The toxicity of copper via the trophic route could be influenced by the composition of the food, and diets naturally impregnated with copper seem to have greater toxicity at lower concentrations than artificially impregnated ones. Thus, our objective was to investigate the effects of copper on juveniles of the Neotropical fish Hoplias malabaricus fed on live prey (Astyanax altiparanae) previously exposed to the metal (20 µg L − 1) for 96 h. The prey fish were given to H. malabaricus every 96 h, totaling 10 doses at the end of the experiment. Thus, after 40 days fish were killed and tissues were sampled. Blood showed to be the only tissue in which copper accumulated. Anemia was found and there was damage to the DNA of erythrocytes. Furthermore, ionic imbalances were observed in plasma. There was an increase in the concentration of Na+ and Cl− and a decrease in Ca2+, which were associated with increased copper uptake in the gastrointestinal tract of fish fed on copper exposed prey. All the antioxidant enzymes evaluated in the gills showed decreased activity compared to the control group. Copper seems to have interfered in the energy metabolism of H. malabaricus, since a lower condition factor and feed conversion efficiency rate were observed in fish fed with copper diet. The present study confirms the trophic route as an important copper toxicity pathway for H. malabaricus and reinforces the idea that metal toxicity can be increased when it is naturally impregnated in the prey tissues, even if the prey has been exposed to the metal only for a short period of time.

PSXV-17 Effects of Dietary Supplementation with Spray Dried Plasma, Dried Intestinal Hydrolysates or Bovine Colostrum on the Growth and Intestinal Health of Weanling Pigs

Abstract In this study, we evaluated the potential of supplementing weaning diets with spray dried porcine plasma, dried porcine intestinal hydrolysates or bovine colostrum to modulate piglet growth, intestinal morphology, antioxidant status, and immunity. At weaning, 96 piglets were housed three piglets per pen in 32 pens. Each pen was assigned to one of the following dietary treatments: 1) basal diet (CTRL); 2) basal diet containing spray dried porcine plasma at 50 g/kg feed (PP); 3) basal diet containing dried porcine intestinal hydrolysates at 50 g/kg feed (PIH); 4) basal diet containing bovine colostrum at 50 g/kg feed (BC). At d 0 and 14, piglets were weighed and feed intake was daily recorded. Eight pigs per treatment (one per pen) were euthanized at d 14 and intestinal samples were collected to evaluate the antioxidant status, intestinal morphology and concentration of TNFα, interleukin (IL) 4, IL17, occludin, caspase 3 and proliferating cell nuclear antigen. Our results showed that PP and BC supplementation increased the final weight and G:F ratio compared with the other dietary treatments, whereas the ADG was greater in the BC group compared with PIH animals (P&amp;lt; 0.05). Furthermore, feeding pigs with the PP diet partially affected the intestinal antioxidant status, as evidenced by the increased concentration of glutathione peroxidase compared with the BC group (P&amp;lt; 0.05). BC supplementation also increased intestinal villus height compared with CTRL and PIH animals, as well as villus to crypt ratio compared with the CTRL group (P&amp;lt; 0.05). No other dietary effect on immunity or intestinal morphology was recorded. This study highlights the potential of dietary supplementation, such as plasma proteins and bovine colostrum, to act as a valid alternative to in-feed antibiotics, to reduce effect of weaning stress and maintain gut health and performance in in the post-weaning period.

342 Effects of Direct fed Microbial Mixtures on Beef Cattle Ruminal Fermentation Characteristics, Intake, and Nutrient Digestibility

Abstract Direct fed microbials (DFM) dietary mixture effects on beef cattle ruminal variables, intake, and apparent total tract digestibility were evaluated. Six ruminally cannulated beef steers (BW = 520 ± 30 kg) were used in a duplicated 3 × 3 Latin square design and offered a steam-flaked corn-based finishing diet ad libitum for three 28-d periods (21-d adaptation and 7-d collection). Treatments assigned were: 1) Control (no DFM mixture; lactose only); 2 and 3) DFM mixtures at distinct concentrations [Mixture A and B, at 2 g/animal-daily (lactose used as carrier)]. Ruminal pH and temperature were measured every 6 min (wireless probes). Ruminal samples were collected at 0, 2, 4, 8, 16, and 23 h post-feeding on d28. Feed and fecal samples (collected once and twice daily, respectively) were composited by period and analyzed. Fecal output was estimated with a dietary internal marker (288-h indigestible-NDF). Data were analyzed using the GLIMMIX procedure of SAS. No treatment × time interactions (P ≥ 0.20) were observed. Steers offered mixture-A experienced 300 min/d less (P = 0.04) time under the ruminal pH 5.6, tended (P = 0.13) for a lesser area under the curve of ruminal pH 5.6, while showing a greater (P = 0.04) ruminal pH average (5.67 vs. 5.50), and tended (P = 0.06) for a lesser ruminal temperature (39.2 vs. 39.4°C) compared to control. The ruminal concentration of NH3-N was greater (P = 0.02) for mixture-A compared with control (10.78 vs. 4.35 mg/dL), while animals offered mixture-B tended (P = 0.07) to be greater (9.11 mg/dL) than control. Steers offered DFM mixture-A increased (P = 0.04) ADF digestibility compared with control (39.8 vs. 54.0%), while not affecting DM intake (P = 0.61). The DFM mixture-A induced a safer ruminal pH environment and encouraging fiber degradation and NH3-N release, while not affecting animal intake

Association between the empirical dietary inflammatory index and cardiorespiratory fitness in Tehranian adults in 2017-2018.

BackgroundInflammatory-related chronic diseases are increasing in Iran with high consumption of a diet containing pro-inflammatory potential and a sedentary lifestyle. The empirical dietary inflammatory index (EDII) was developed as a tool to assess dietary effects on systemic inflammation. We examined the hypothesis that specific dietary patterns reflecting systemic inflammation are associated with cardiorespiratory fitness (CRF) in Tehranian adults.MethodsThis cross-sectional study was carried out on 270 adults who are residents of Tehran. Dietary intake was assessed using a 168-item valid and reliable food frequency questionnaire. The EDII score was developed according to participant dietary intakes of 21-item pre-defined food groups. CRF was assessed by using a graded exercise treadmill test. Anthropometric measurements were assessed using standard methods. To discover the association between CRF and EDII, we used multivariable logistic regression analysis.ResultsThose who were in the third tertile of the EDII had 57% lower odds of having better VO2Max (ml/kg/min) than those in the first tertile (OR: 0.43; 95% CI: 0.16, 1.12, p = 0.01). There were no significant differences between tertiles of the EDII score in terms of VO2 (L·min) and VO2 (LBM) before and after adjusting for confounders. There was a significant decrease in VO2Max (ml/kg/min) across tertiles of the EDII after controlling for covariates (p-value = 0.04). There was a significant inverse association between the EDII score and VO2Max (ml/kg/min) (β = −0.35, p = 0.001).ConclusionsOur finding demonstrated that a higher EDII might be associated with lower CRF in Tehranian adults. Prospective studies are needed to shed light on the causal link between the EDII and CRF.