Determination Of Propylene Glycol Research Articles

Simultaneous Determination of Propylene Glycol, Glycerol and Lactic Acid in Rat Plasma and Serum by Gas Chromatography–mass Spectrometry

A simple and rapid gas chromatographic–mass spectrometric (GC–MS) method using selected ion monitoring mode was developed for simultaneous determination of propylene glycol, glycerol and lactic acid in rat plasma and serum. The method employed a single-step derivatization using bis(N,O-trimethylsilyl) trifluoroacetamide with trimethylchlorosilane and required only a small amount of plasma or serum (100 μL). The calibration curves showed linearity with the correlation coefficients of 0.996–0.999. To validate the method, the intra- and inter-day repeatability of the proposed method in rat plasma was evaluated. Recovery was assessed by the standard addition method. To test the applicability of the method, twenty plasma samples in two different time points were analyzed and levels of both propylene glycol and lactic acid in plasma were found to increase after exposure to the propylene glycol aerosol (500 mg/kg). With satisfactory repeatability and recovery the proposed GC–MS method can be successfully applied for analysis of plasma and serum samples.

Determination of Propylene Glycol in Low Volume Plasma and Urine Samples of Neonates by LC with Photodiode Array Detection

In order to enhance the sensitivity and to develop a method suitable for quantification of propylene glycol (PG) in low volume neonate plasma and urine samples, several steps in earlier described high performance liquid chromatographic methods were optimised. Chromatographic separation on a reversed-phase column and ultraviolet detection resulted in cleaner chromatograms without interfering compounds. Linearity of the standard curves was validated in the concentration range 0.25–50 mg L−1. The lower limit of quantification was 20 times lower than in earlier described methods. Presented method was suitable for quantification of PG concentrations in low volume neonate plasma (15–46 mg L−1) and urine samples (20–175 mg L−1) enabling us to document very low renal versus non-renal contribution of PG clearance in neonates.

Improvement of Sensitivity for the Determination of Propylene Glycol in Rat Plasma and Lung Tissue Using HPLC/Tandem MS and Derivatization with Benzoyl Chloride

Propylene glycol (PG), a simple alcohol, is a commonly used vehicle for aerosol dosage formulations. Quantification of PG in plasma and lung tissue is, therefore, important for new drug development. We describe a highly sensitive and selective method for the quantitative determination of PG in rat plasma and lung tissue, using liquid chromatography (LC) with positive atmospheric pressure chemical ionization (APCI) tandem mass spectrometry (MS) detection. Propylene glycol and the internal standard (IS) 1,4‐butanediol were derivatized with benzoyl chloride under alkaline conditions to enhance the sensitivity of detection. Ionization efficiency was improved following derivatization. The limits of detection (LOD) for rat plasma and rat lung tissue were 0.269 µg/mL and 1.12 µg/g, respectively. The LOQs for rat plasma and lung tissue were 0.448 µg/mL and 1.62 µg/g, respectively. Calibration curves were linear from 2 to 4000 µg/mL for rat plasma (r = 0.9990) and from 2 to 2400 µg/g for rat lung tissue (r = 0.9985). The assay showed intra‐assay and inter‐assay precision (%RSD) of 3.8–4.8% (n = 6) and 4.2–6.6% (n = 18) for rat plasma, 3.8–4.0% (n = 6) and 6.5–7.4% (n = 18) for rat lung tissue, respectively. The percent inaccuracy for inter‐assay results in rat plasma were 2.5–4.7% and in rat lung tissue were 1.3–6.8% for different concentrations. All plasma and lung tissue samples demonstrated acceptable freeze/thaw stability, bench stability, and prepared sample stability over a 24 hours period. An alternative derivatizing method using perfluorooctanoyl chloride with negative APCI/MS detection was investigated. Although strong fragment ions of the derivatives could be detected, the feasibility of this method was limited by sample preparation. Additionally, fragment ions produced from the perfluorooctanoyl moiety lacked selectivity. The benzoyl chloride method is proved to be more sensitive, selective, and robust.

Gas-liquid chromatographic determination of propylene glycol in plasma and urine.

We describe a gas-chromatographic assay for propylene glycol in human plasma and urine. The method is sensitive enough to allow quantification of the compound at concentrations observed clinically. It requires no derivative preparation and takes only 13 min of chromatographic time.

Collaborative Study of the Determination of Propylene Glycol, Glycerol, and Triethylene Glycol in Tobacco

Abstract Collaborative study was carried out on a method for determination of propylene glycol, glycerol, and triethylene glycol in tobacco. The method involves gas chromatography of the methanolic extract of tobacco on a 42 × 3/16″ column packed with 5% Carbo wax 20M-terephthalic acid on 60-80 mesh 1:1 Diatoport S-Chromosorb 101 mixed support. 1,3-Butylene glycol is used as an internal standard. The oven temperature is programmed from 90 to 240°C at 15°/min. Results for propylene glycol and glycerol gave coefficients of variation <10%. Results for triethylene glycol in cut tobacco at mean concentration levels of 0.95 and 1.30% gave coefficients of variation of 9.24 and 6.93%, respectively. At a mean concentration level of 0.54% triethylene glycol, the coefficient of variation was 20.8%. The method has been adopted as official first action.

Collaborative Study on the Determination of Propylene Glycol, Glycerine, and Triethylene Glycol in Tobacco

Abstract Propylene glycol, glycerine, and triethylene glycol were extracted from 10.0 g tobacco by shaking 1 hr with methanol. All three polyols were determined simultaneously by GLC of the supernatant extract, using a 42 × 3/16″ column of 1 + 1 Diatoport S-Chromosorb 101 coated with 5% Carbowax 20M-terephthalic acid. The column temperature was programmed at 90−240°C; anethole was used as the internal standard. The method was studied collaboratively by 11 laboratories. Systematic errors have been reduced to acceptable levels except in samples containing very low or high concentrations of a given humectant.

Collaborative Study on the GLC Determination of Propylene Glycol in Cosmetics

Abstract A method for the determination of propylene glycol in cosmetics by GLC, using Chromosorb 101 as a column packing, was submitted to collaborative study. Four samples were analyzed at two levels of concentration. Results for the six collaborators show recoveries of 95-108% for samples containing from 20.0 to 40.0 mg propylene glycol. The standard deviation varied from 0.54 to 1.67 and the coefficient of variance from 2.8 to 4.7%. It is recommended that the method be adopted as official first action.

Determination of Propylene Glycol in Cosmetics by Gas Chromatography

Abstract Propylene glycol in cosmetics can be determined by co-distillation in water followed by GC measurement of the distilled propylene glycol on a Porapak Q column and a hydrogen flame detector. The procedure has been successfully applied to a number of commercial samples; 14 of 15 sample recoveries were between 95 and 102%, the other recovery was 92%.

The determination of propylene glycol in tobacco by cellulose-column chromatography

The determination of propylene glycol in tobacco by cellulose-column chromatography

Report on the Determination of Propylene Glycol and Glycerine in Imitation Vanilla Flavors by a Chromatographic Procedure

Report on the Determination of Propylene Glycol and Glycerine in Imitation Vanilla Flavors by a Chromatographic Procedure

Report on the Determination of Propylene Glycol in Vanilla Extracts

Report on the Determination of Propylene Glycol in Vanilla Extracts