Determination Of Phenolic Compounds Research Articles

Determination of Phenolic Compounds in Blue Corn Flour (Zea mays L.) Produced and/or Metabolized by Colletotrichum gloeosporioides in a Fermentation Process

Phenolic compounds are secondary metabolites produced by plants, and their study has been increased in recent years due to their ability to improve human health. The aim of this work was the determination of phenolic compounds presents in blue corn flour before and after a fermentation process, where different proportions were used of blue corn (Zea mays L.) flour and Czapek Dox culture medium (90 mL of culture medium with 10 g of blue corn flour, 80 mL of culture medium with 20 g of blue corn flour and 70 mL of culture medium with 30 g of blue corn flour) and were fermented at 3 different times (20, 25 and 30 days) with the Colletotrichum gloeosporioides fungus. A determination of the phenolic compounds was carried out with five standard solutions, which were cyanidin 3-glucoside (CYA), pelargonidin 3-glucoside (PEL), chlorogenic acid (CLA), quercetin (QRC) and cinnamic acid (CA). The obtained results showed the presence of CA and PEL. The most abundant phenolic compound in the fermented samples was CLA over the naturally occurring compounds in blue corn, which are CYA and PEL. QRC was the phenolic compound with the lowest concentration in blue corn flour samples fermented with Colletotrichum gloeosporioides.

A μ-QuEChERS method combined with UHPLC-MS/MS for the analysis of phenolic compounds in red pepper varieties

In this study, a method based on QuEChERS method miniaturization followed by UHPLC-MS/MS analysis was used for the first time to determine phenolic acids and flavonoids in red pepper (Capsicum spp.). Under the optimized extraction and analysis conditions, fourteen phenolic compounds in eleven varieties of red pepper belonging to four domesticated species, C. baccatum L., C. chinense Jacq., C. frutescens L., and C. annuum L., were evaluated. 4-Hydroxybenzoic, p-coumaric, vanillic and ferulic acids, and naringenin were the phenolic compounds found in all varieties evaluated. Among them, vanillic and ferulic acids and naringenin were found in higher amounts, accounting for 92.2% of the total phenolic compounds quantified in all varieties evaluated. Overall, this study reveals relevant and innovative data about the composition of the phenolic compounds of red peppers, especially for those varieties little or not yet studied, such as “Cambuci,” “Dedo-de-moça,” “Big Red Mama,” “Brazilian Moruga,” “Biquinho,” “Naga Morich,” “Fatalii Gourmet Jigsaw,” and “Bhut Jolokia Assam”, which will boost its potential uses in agro-food, cosmetic and pharma industry, as well as in health promotion. Furthermore, data support that the μ-QuEChERS method was more cost-effective, less time-consuming, and environmentally friendly, constituting a promising trend for phenolic compounds determination in plant matrices.

Field Study and Chemical Analysis of Plant Waste in the Fez-Meknes Region, Morocco

Throughout the entire world, the biomass plant remains an important source of renewable energy. However, in Morocco, the energy recovery of this biomass is little or badly exploited compared to other solar, hydraulic, and wind resources. The aim of this study is to know the extent to which Moroccan companies are involved in the valorization of green waste and to identify among the latter those that have great energy and industrial value. The field investigation was carried out with the use of a questionnaire to different sectors of activity. The chemical analyses of the waste samples were carried out by different methods: Van Soest to investigate the fiber content, dinitrosalicylic acid and phenol-sulfuric acid to determine sugars, while the Folin–Ciocalteu method was employed for the determination of phenolic compounds. These are the ASTM standard methods to determine elemental, proximate composition, and calorific value (CV). The results of this survey showed that solid vegetable waste is diverse and represents 68.4% of the total green waste, of which 98% is not treated. Moreover, the chemical analysis displayed that forestry waste (FW), extracted parts wastes (EPW), and unused parts wastes (UPW) of medicinal and aromatic plants have high contents of cellulose (respectively 34.75, 48.44, and 54.19%) and hemicelluloses (28.44, 27.19 and 28.50%) and containing low amounts of lignin and phenolic compounds compared to olive waste (OW), olive pomace (OP), and household waste (HW). Almost all biomass wastes, except HW, have a low moisture (<12%), ash content less than 5.1%, a significant percentage of C and H, and CV between 14.5 and 21.6 MJ/Kg. The PCA analysis showed a discrepancy in terms of components between the set formed by FW, UPW, and EPW with other solid waste. In conclusion, FW, UPW, and EPW, specially can be potentially energetic biomass and valorized together in the form of a mixture.

Determination of Biochemical Characteristics, Antioxidant Activities, and Individual Phenolic Compounds of 13 Native Turkish Grape Juices

Determination of Biochemical Characteristics, Antioxidant Activities, and Individual Phenolic Compounds of 13 Native Turkish Grape Juices

Сравнительная оценка водных экстрактов прополиса, приготовленных разными способами

Abstract. In the course of the study, an updated database of the content of biologically active substances in aqueous extracts of propolis prepared at different temperatures and in different ways is presented. The purpose of the work was to select the optimal modes of preparation of aqueous propolis extracts, to determine the content of biologically active substances in freshly prepared propolis extracts and during storage. The studies were carried out in the laboratory of the Federal State Budgetary Scientific Institution “FNC of beekeeping”. The relevance of the work lies in the development of methods for extracting aqueous extracts of propolis at different temperatures (t = 20 °C, t = 93 °C), the use of ultrasound. Scientific novelty for the first time biologically active substances were determined by the method of determining antioxidant activity. Methods. The yield of extractives (mass fraction of solids) was determined by drying to constant weight according to GOST 28886-90. Determination of unsaturated compounds was performed according to GOST 28886-90. The determination of the hydrogen index (pH) was performed according to the Pharmacopoeia (1987) in our modification GOST 28886-90. Determination of flavonoid and other phenolic compounds was performed by photometry, according to GOST 28886-90 (total flavonoid compounds). Determination of flavan, flavanones, flavonols was carried out according to the method presented in the “Guidelines for methods of quality control and safety of biologically active food supplements” R 4.1.1672-0, 2004 in terms of routine. The antioxidant activity of propolis aqueous extracts was assessed by a method based on the reaction of KMnO4 in the presence of 0.24 M H2SO4 with the studied propolis solution, followed by conversion to quercetin in 1 ml or 1 g of the drug (patent 2170930). Results. The amount of solids during storage in the aqueous extract of propolis (method 1) decreased from 0.23 to 0.15 %; in an aqueous extract prepared using ultrasound (method 3) increased from 0.21 % to 0.23 %. The amount of solids in propolis aqueous extracts prepared by methods 2 and 4 decreased during storage from 0.55 to 0.49 % (method 2) and from 0.6 to 0.59 % (method 4).

Rapid Sample Screening Method for Authenticity Controlling of Vanilla Flavours Using Liquid Chromatography with Electrochemical Detection Using Aluminium-Doped Zirconia Nanoparticles-Modified Electrode.

A rapid and sensitive technique for frauds determination in vanilla flavors was developed. The method comprises separation by liquid chromatography followed by an electrochemical detection using a homemade screen-printed carbon electrode modified with aluminium-doped zirconia nanoparticles (Al-ZrO2-NPs/SPCE). The prepared nanomaterials (Al-ZrO2-NPs) were characterized by using X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy dispersive X-ray (EDX). This method allows for the determination of six phenolic compounds of vanilla flavors, namely, vanillin, p-hydroxybenzoic acid, p-hydroxybenzaldehyde, vanillyl alcohol, vanillic acid and ethyl vanillin in a linear range between 0.5 and 25 µg g−1, with relative standard deviation values from 2.89 to 4.76%. Meanwhile, the limits of detection and quantification were in the range of 0.10 to 0.14 µg g−1 and 0.33 to 0.48 µg g−1, respectively. In addition, the Al-ZrO2-NPs/SPCE method displayed a good reproducibility, high sensitivity, and good selectivity towards the determination of the vanilla phenolic compounds, making it suitable for the determination of vanilla phenolic compounds in vanilla real extracts products.

Extraction and determination of phenolic compounds in Chinese teas using a novel compound salt aqueous two-phase system coupled with multivariate chemometric methods

The phenolic compounds are the primary ingredients contributing to the color, fragrance, and health care functions of tea. The present study sought to extract the phenolic compounds from Chinese tea samples using a novel compound salt aqueous two phase system (ATPS) with iso-propanol and two inorganic salts (sodium citrate and ammonium sulfate). The results showed that the analytes were mainly distributed in the alcohol-rich top phase within the recovery range of 82.54–110.73%. Additionally, the alternating trilinear decomposition (ATLD) algorithm combined with HPLC-DAD was employed for the simultaneous rapid quantitative analysis of six phenolic compounds in four Chinese tea samples within 7.0 min. Based on the quantitative data obtained by the proposed ATPS-ATLD method, the principal component analysis (PCA) and hierarchical cluster analysis (HCA) methods were adopted to classify and analyze the four types of Chinese teas. Overall, the proposed ATPS-ATLD method could be a potential approach for distinguishing the types of Chinese teas.

Chromatographic analysis of water and water-alcohol extracts of Echinacea purpurea L. obtained by various methods

In phytotherapy, Echinacea purpurea L. is generally used in the form of water-alcohol extracts, which contain phenylpropanoids exhibiting anti-inflammatory, hypoglycemic and moderate antiproliferative effects. The relevance of using plants of the genus Echinacea as a phytomaterial is due to the high demand of consumers; nowadays, the level of sales of water-alcohol extracts in the world is quite high. Considering the importance of using these plants, the present work aimed to determine phenolic compounds in water-alcohol extracts of echinacea obtained by various extraction methods. The biological activity of echinacea is related to hydroxycinnamic acids and their derivatives (chlorogenic, caftaric and chicoric acids). In this study, conditions for the identification and determination of phenolic compounds using such chromatographic methods as HPLC-DAD-MS and GC–MS allowing to expand the range of target analytes in water-alcohol extracts of echinacea have been proposed. A number of unknown compounds of phenolic nature and monosaccharides have been identified in water extracts of echinacea by implementing the SPAD technique on a sorbent for solid-phase extraction - Strata C18-E. The effectiveness of various extraction methods and their influence on the composition of extracted phenolic compounds from plant raw materials was studied. Subcritical extraction at 120 °C was established to provide maximum extraction of rutin, caffeic and caftaric acids. Chlorogenic and chicoric acids are most effectively extracted by traditional methods described in the Pharmacopoeia. The influence of growing conditions on the total content of phenolic compounds in echinacea was studied; the increase in the altitude above sea level results in higher contents of phenolic compounds. The assessment of the content of phenolic compounds in industrially manufactured preparations based on echinacea was carried out. The possibility of using the ratio of chicoric to caftaric acids as an indicator of the quality and authenticity of extracts and preparations based on them was shown.

A Comprehensive Survey of Phenolic Constituents Reported in Monofloral Honeys around the Globe.

The aim of this review is to provide a comprehensive overview of the large variety of phenolic compounds that have to date been identified in a wide range of monofloral honeys found globally. The collated information is structured along several themes, including the botanical family and genus of the monofloral honeys for which phenolic constituents have been reported, the chemical classes the phenolic compounds can be attributed to, and the analytical method employed in compound determination as well as countries with a particular research focus on phenolic honey constituents. This review covers 130 research papers that detail the phenolic constituents of a total of 556 monofloral honeys. Based on the findings of this review, it can be concluded that most of these honeys belong to the Myrtaceae and Fabaceae families and that Robinia (Robinia pseudoacacia, Fabaceae), Manuka (Leptospermum scoparium, Myrtaceae), and Chestnut (Castanea sp., Fagaceae) honeys are to date the most studied honeys for phenolic compound determination. China, Italy, and Turkey are the major honey phenolic research hubs. To date, 161 individual phenolic compounds belonging to five major compound groups have been reported, with caffeic acid, gallic acid, ferulic acid and quercetin being the most widely reported among them. HPLC with photodiode array detection appears to be the most popular method for chemical structure identification.

Determination of phenolic compounds and antioxidant potential of aqueous extracts of Curcuma longa L., Piper nigrum L. and Cuminum cyminum: an experimental and a quantum-mechanical study

Objectives: Evaluation of phenolic compounds and antioxidant activities of aqueous extracts of C. longa, P. nigrum and C. cyminum. In addition to proposing a quantum-mechanical model to evaluate the antioxidant activity. Methods: The aqueous extracts were prepared using roots of the Curcuma longa L., seeds of the Piper nigrum L. and seeds of Cuminum cyminum. The extracts were subjected to tests to detect and quantify phenolic compounds and to assess their antioxidant capacity by different methods. Furthermore, in order to investigate the electronic nature of the antioxidant activity of the main compounds present in these extracts, frontier molecular orbitals (FMOs) were obtained by DFT/B3LYP/6-31G(d,p) level of theory. Results: After statistical analysis of the results, a greater number of phenolic compounds and better antioxidant activity was identified in the aqueous extracts of cumin (C. cyminum) in all three assays performed, when compared to the other extracts tested. The theoretical model based on the Pietro method is in agreement with the experimental results. Conclusion: This study has an innovative proposal with the trivial antioxidant activity combined with theoretical quantum-mechanical calculations that can serve to reduce costs and time and to predict the antioxidant activity of subsequent studies.

The role of MOF based nanocomposites in the detection of phenolic compounds for environmental remediation- A review

Phenolic compounds would be the emerging pollutant by 2050, because of their wide spread applicability in daily life and therefore the adoption of suitable detection methods in which identification and separation of isomers is highly desirable. Owing to the fascinating features, Metal-organic framework (MOF), a class of reticular materials holds a large surface area with tunable shape and adjustable porosity will provide strong interaction with analytes through abundant functional groups resulting in high selectivity towards electrochemical determination of phenolic isomers. Nevertheless, the sensing performance can still be further improved by building MOF network (intrinsic resistance) with functional (conducting) materials, resulting in MOF based nanocomposite. Herein, this review provides the summary of MOF based nanocomposites for electrochemical sensing of phenolic compounds developed from 2015. In this review, we discussed the demerits of pristine MOF as electrode materials, and the requirement of new class of MOF with functional materials such as nanomaterials, carbon nanotubes, graphene and MXene. The history and evolution of MOF nanocomposite-based materials are discussed and also featured the impressive physical and chemical properties. Besides this review discusses the factors influencing the conducting pathway and mass transport of MOF based nanocomposite for enhanced sensing performance of phenolic compounds with suitable mechanistic illustrations. Finally, the major challenges governing the determination of phenolic compounds and the future advancements required for the development of MOF based electrodes for various applications are highlighted.

Determination and Comparison of Phenolic Compound Content and Antimicrobial Activity of Some Propolis Samples in Turkey

Objective: Ethanol extraction is the most popular technique for the production of propolis extracts. However, this method may not be suitable for various clinical conditions. Based on it, we composed a trial product with an olive-oil extraction as an alternative method. Furthermore, we crafted combinations to reinforce and synergize the antimicrobial activity of the trial propolis product. Finally, we compared our trial products with the existing marketing products in Turkey. The present study aimed to determine chemical compounds and the antimicrobial activity of some propolis samples selected from Turkey and compare the mentioned features with the olive-oil- trial products we composed for the study.
 Material-Methods: Four different samples, as trial and final products, were crafted for the study. Trail products conducted as sample 1 to 4 (S1, S2, S3 and S4). The trial products were compared with the four other propolis and propolis-containing combined products currently exciting on the market. Four different trademarks were used, and the Trademarks (TM) was called TM1, TM2 TM3, and TM4. Determination of Total Phenolic Compound (TPC) was analyzed according to the Folin-Ciocalteau method. The antimicrobial activity test was determined according to the Kirby-Bauer method.
 Results: The highest TPC ratio was detected in the trademark 2 (TM2), and the lowest TPC ratio was determined in the TM4 samples, 19553.12 GAE mg/L and 740.9 GAE mg/L, respectively. The TPC ratio of the final trial product sample 4 (S4) was defined as 6519.3 GAE mg/L. The highest inhibitation zone against E. coli, K. pneumoniae, S. aureus strains was observed in S1 (the oleuropein-containing trial product). The highest inhabitation zone against C. albicans and C. krusei yeasts was observed in TM1 and S4 (the oleuropein and boron-containing trial propolis product) samples. 
 Conclusion: The S4 product, containing boron, oleuropein, and propolis, had a higher inhibitation zone diameters compared to the commercial brands. Furthermore, all the propolis products analyzed in this study had rich phenolic components; the curative and beneficial impacts of phenolic components on health merit further investigations.

Phenolic Compounds and Antimicrobial Activity of Olive (Olea europaea L.) Leaves

Background: Olive leaves are of great interest, especially in traditional medicine. The polyphenols contained in olive leaves play an important role in this respect, as they have anti-carcinogenic, anti-inflammatory and anti-microbial properties. Olive leaves share phenolic compounds with other plants, but they also contain phenolic compounds belonging to the Oleaceae family. Methods: We report the determination of phenolic compounds in olive leaves by HPLC and the evaluation of their in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Enterobacter cloacae, Proteus mirabilis and Salmonella typhimurium. Result: The results reveal that the olive leaves may constitute a good source of antimicrobial agents. The high performance liquid chromatography (HPLC) analysis showed the presence of five phenolic compounds: oleuropein, ascorbic acid, rutin, catechinand verbascoside and for the first time ascorbic acid. At low concentrations, olive leaf extracts showed an unusual antibacterial action, which suggests their great potential as nutraceuticals, particularly as a source of phenolic compounds.

Comparative Determination of Phenolic Compounds in Arabidopsis thaliana Leaf Powder under Distinct Stress Conditions Using Fourier-Transform Infrared (FT-IR) and Near-Infrared (FT-NIR) Spectroscopy.

The increasing interest in plant phenolic compounds in the past few years has become necessary because of their several important physicochemical properties. Thus, their identification through non-destructive methods has become crucial. This study carried out comparative non-destructive measurements of Arabidopsis thaliana leaf powder sample phenolic compounds using Fourier-transform infrared and near-infrared spectroscopic techniques under six distinct stress conditions. The prediction analysis of 600 leaf powder samples under different stress conditions (LED lights and drought) was performed using PLSR, PCR, and NAS-based HLA/GO regression analysis methods. The results obtained through FT-NIR spectroscopy yielded the highest correlation coefficient () value of 0.999, with a minimum error (RMSEP) value of 0.003 mg/g, based on the PLSR model using the MSC preprocessing method, which was slightly better than the correlation coefficient () value of 0.980 with an error (RMSEP) value of 0.055 mg/g for FT-IR spectroscopy. Additionally, beta coefficient plots present spectral differences and the identification of important spectral signatures sensitive to the phenolic compounds in the measured powdered samples. Thus, the obtained results demonstrated that FT-NIR spectroscopy combined with partial least squares regression (PLSR) and suitable preprocessing method has a solid potential for non-destructively predicting phenolic compounds in Arabidopsis thaliana leaf powder samples.

Comparative Extraction of Phenolic Compounds from Olive Leaves Using a Sonotrode and an Ultrasonic Bath and the Evaluation of Both Antioxidant and Antimicrobial Activity.

A sonotrode ultrasound-assisted extraction of phenolic compounds from olive leaves has been developed using a Box–Behnken design to optimize the effects of solvent composition and ultrasound parameters. The determination of single phenolic compounds was performed by HPLC–MS and the highest recovery in total compounds, oleuropein and hydroxytyrosol was achieved using EtOH/H2O (55:45, v/v), 8 min and 100% of amplitude. The optimal conditions were applied on leaves from seven olive cultivars grown under the same conditions and the results were compared with those found by using a conventional ultrasonic bath, obtaining no statistical differences. Moreover, antioxidant activity by FRAP, DPPH and ABTS in these olive leaf extracts was evaluated and they exhibited a significant correlation with oleuropein and total phenolic content. All cultivars of olive leaf extracts were found to be active against S. aureus and methicillin-resistant S. aureus with minimum bactericidal concentration (MBC) values) that ranged from 5.5 to 22.5 mg mL−1. No extracts showed antimicrobial activity against C. albicans. The percentages of mycelium reduction in B. cinerea ranged from 2.2 and 18.1%. Therefore, sonotrode could be considered as an efficient and fast extraction technique that could be easily scaled-up at industrial level, thus allowing for olive leaves to be revalorized.

Voltammetric Determination of Total Phenolic Compounds in Cotton Leaves of Gossypium barbadense and Gossypium hirsutum Challenged with Spodoptera cosmioides (Walker)

A voltammetric method for phenolic compounds determination detected differences between Gossypium hirsutum and Gossypium barbadense, injured or not by caterpillars. G. barbadense seeds collected in Amazon were multiplied and compared to a commercial Brazilian variety (G. hirsutum). Plants were conducted in a net protected house for two assays where: i) floral buds were inoculated with boll weevil eggs and ii) leaves were infested with Spodoptera cosmioides (Walker) and leaf phenolic compounds determined by a voltammetric method based on the differential pulse technique and glassy carbon electrode. The proportion of emerging boll weevils was greater in G. hirsutum (0.24) than in G. barbadense (0.14). G. hirsutum non infested older leaves presented a higher amount of total phenolics than the younger leaves. For the infested plants, total phenolic levels were similar in both developmental stages and to the not infested younger leaves. The most intense current peak in the voltammetric profile was at the oxidation potential of 0.5 V for all samples, and more intense for G. hirsutum older leaves. Another less intense current peak at 0.8 V, found at all profiles, was great for G. barbadense attacked by caterpillars. Compounds coincident with these peaks may be related to insect tolerance.

Determination of Both Total Phenolic Compound and Antioxidant Capacity of Herbal Teas Using Smartphone Digital Imaging

In this work, a low cost and portable optical sensor using a smartphone camera for antioxidant analysis was demonstrated. A platform was designed and installed on a smartphone as an attachment for the assay solution, a macro lens, and a white LED. The proposed confi guration allowed the illumination from the white LED to pass through the solution before entering the smartphone camera. After the color of the solution was captured by the camera, the image was sent to a laptop by Bluetooth and the Red, Green and Blue (RGB) intensity values were determined using ImageJ software. The proposed platform was used to assess the antioxidant capacity of standard Trolox, gallic acid, and a range of herbal teas using 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazine (DPPH) assay, and then calculated as an IC50 value. The total phenolic compounds (TPC) of these samples were also evaluated using Folin-Ciocalteu (FC) assay, and presented as gallic acid equivalents (GAE). The results of the three assays when using the smartphone were in good agreement with those from a commercial UV-Vis spectrophotometer. For the three assays, a good linearity and precision were obtained R2 > 0.995, and % RSD was lower than 2% (n = 5), respectively. For FC assay, the linear range and the limit of detection were 0.0-7.0 mg/L and 0.20 mg/L, consequently.

Determination of Phloridzin and Other Phenolic Compounds in Apple Tree Leaves, Bark, and Buds Using Liquid Chromatography with Multilayered Column Technology and Evaluation of the Total Antioxidant Activity.

Apples are known to be a rich source of phenolic compounds, however detailed studies about their content in the individual parts of apple trees are reported rarely. For this purpose, we tested various stationary phases for the determination of phenolic compounds in leaves, bark, and buds. Phloridzin, phloretin, chlorogenic acid, rutin, and quercitrin were analyzed with high performance liquid chromatography coupled with diode array detection. A YMC Triart C18-ExRS 150 × 4.6 mm, 5 µm particle size analytical column with multilayered particle technology was used. The separation was performed with a mobile phase that consisted of acetonitrile and 0.1% phosphoric acid, according to the gradient program, at a flow rate of 1 mL/min for 12.50 min. The concentration of phenolic compounds from 13 cultivars was in the range of 64.89–106.01 mg/g of dry weight (DW) in leaves, 70.81–113.18 mg/g DW in bark, and 100.68–139.61 mg/g DW in buds. Phloridzin was a major compound. The total antioxidant activity was measured using flow analysis and the correlation with the total amount of phenolic compounds was found. This finding can lead to the re-use of apple tree material to isolate substances that can be utilized in the food, pharmaceutical, or cosmetics industries.

Development of a Polyphenol Oxidase Biosensor from Corn Tassel Extract for Determination of Phenolic Compounds in Industrial Waste Water

Development of a Polyphenol Oxidase Biosensor from Corn Tassel Extract for Determination of Phenolic Compounds in Industrial Waste Water

Determination of Phenolic Compounds Using HPLC-UV Method in Wild Fruit Species

Plant parts of some spontaneous fruit species were analyzed by HPLC-UV method to determine their content in phenolic compounds. Buds, leaves, flowers, and fruits were harvested from 11 wild fruit species of medicinal, food, and therapeutic interest: European crab apple, European wild pear, blackthorn, dog rose, elder, dewberry, wild blackberry, cornelian cherry, red hawthorn, black hawthorn, and green strawberry. The studied species were analyzed for the presence and amount of phenolic compounds: flavonoids from the subclasses flavanols (catechin hydrate and epicatechin) and flavonols (rutin, myricetin, quercetin, and kaempferol) together with phenolic acids belonging to the hydroxybenzoic acid subclass (ellagic, gallic, syringic, vanillic, and salicylic) and hydroxycinnamic acids (caffeic, ferulic, sinapic, p-coumaric, neochlorogenic, and chlorogenic). The obtained results confirm the presence of bioactive compounds in different plant parts in wild fruit species. The variability identified from one species to another, the different compositions of phenols within the species, provides useful data on the role of these plant parts in the human body.