Detection Of Bisphenol A Research Articles

Polyaniline-nanofiber-modified screen-printed electrode with intermediate dye amplification for detection of endocrine disruptor bisphenol A

We develop a bisphenol A (BPA) detection sensor using a laccase-immobilized polyaniline (PANI)-doped screen-printed electrode (SPE). BPA detection is carried out by immobilizing laccase on a 4-mm-diameter working electrode. When laccase is immobilized on the PANI/SPE, the activity of the enzyme varies according to the immobilization method. After immobilizing the enzyme on the PANI/SPE, we perform cyclic voltammetry measurements with different BPA concentrations. The detection range and the response time for BPA detection are found to be 1 ppb to 1 ppm and ~100 s intervals, respectively. In the study, the electrode potential is varied from 2 to -2 V at a scan rate of 300 mV/s, and a potential of -1.175 V is applied. In order to increase the sensitivity of laccase/PANI/SPE, we perform experiments with the addition of dyes that are used to transfer electrons quickly at the stage of immobilization. Toluidine blue, Reactive Red 120, and bromothymol blue are used as electron transfer mediators. The linear detection range of the laccase/PANI/SPE is found to be 1 ppb to 10 ppm and the lower detection limit is 0.01 ppb. We use amperometry to observe the change in current with change in the BPA concentration over time.

Rapid determination of bisphenol A in environmental water by solid phase extraction-ultra performance convergence chromatography

A rapid method based on solid phase extraction-ultra performance convergence chromatography was established for the determination of bisphenol A (BPA) in environmental water. The chromatographic conditions and solid phase extraction conditions were investigated, and the rapid and effective analysis of BPA could be achieved in 4 min. The results showed that the method has a good linearity with BPA in the range of 0.20-100 mg/L with correlation coefficient (R2) of 0.9996. Furthermore, the limit of detection (LOD) was 0.01 mg/L. The spiked recovery (R) and relative standard deviation (RSD) were 89.7%∼109.2% and 2.78%∼4.66%, respectively. The method is rapid, simple, accurate and reliable, which can provide technical reference for the detection of BPA in environmental water.

Fluorometric detection of bisphenol A using β-cyclodextrin-functionalized ZnO QDs.

The estrogenic property of bisphenol A (BPA) leads to potential adverse health and ecological effects. A simple, selective, and cost-effective sensor capable of detecting BPA would have a noteworthy relevance for the environmental system. The present work illustrates the synthesis and characterization of β-cyclodextrin (β-CD) functionalized zinc oxide (ZnO) quantum dots (QDs) for the selective detection of BPA. BPA has a fluorescence quenching effect on functionalized ZnO QDs, and the decrease in fluorescence intensity is associated with the BPA concentration between 2 and 10μM. Under the optimum reaction condition, a good linear correlation was obtained between relative fluorescence-quenching intensity of β-cyclodextrin-functionalized ZnO QDs and BPA concentration (R2 = 0.9891). The lower detection limit of functionalized QDs for BPA was estimated to be 0.19μM, which is lower than the toxic limits in aquatic biota. The fluorescence-based detection of BPA may be ascribed to the electron transfer mechanism, which is elucidated with scientific details from the literature.

Electrochemical sensor for determination of bisphenol A based on MOF-reduced graphene oxide composites coupled with cetyltrimethylammonium bromide signal amplification

A sensitive electrochemical sensor was fabricated for the rapid determination of bisphenol A (BPA) based on cerium-centered metal-organic framework electrochemically reduced graphene oxide composite (Ce-MOF-ERGO) coupled with cetyltrimethylammonium bromide (CTAB) signal amplification. The Ce-MOF-ERGO was synthesized by electrochemical reduction of Ce-MOF-graphene oxide (GO) composite. Under the optimized conditions, the proposed electrochemical sensor exhibited a linear response for BPA in concentration range 3 nM to 10 μM with a detection limit of 1.9 nM. The excellent performance of the proposed sensor was attributed to the high conductivity, large surface area, and catalytic activity of Ce-MOF-ERGO composite. Moreover, the oxidation signals of BPA on Ce-MOF-ERGO-modified electrode were further enhanced significantly after addition of CTAB in electrolyte solution. Based on the dual signal amplification strategy, the sensor exhibited high sensitivity for BPA detection and was successfully applied to detect BPA in real samples with good recoveries.

Surface Plasmon Resonance Boosting Photoelectrochemical System for Ultrasensitive Detection of Bisphenol A

A semiconductor-metal-based photoelectrochemical (PEC) method is developed for bisphenol A (BPA) detection. The composite electrode is prepared by spin-coating of tin oxide (SnO2) nanoparticles on the surface of indium-tin oxide (ITO), followed by a controllable sputtering of gold nanoparticle (Au NPs). The introduction of Au NPs greatly improves the PEC response of the electrode, especially in the presence of tris (2,2′-bipyridine) ruthenium (II) hexafluorophosphate, Ru(bpy)3(PF6)2. Since the oxidation potential of BPA is lower than that of photo-generated Ru3+, BPA acts as an electron donor to reduce Ru3+ back to Ru2+ for the next round, which in turn establishes the relationship between the photocurrent and BPA concentration. Owing to the Plasmon effect of Au NPs, the energy level matching between SnO2 and Ru(bpy)32+, and the appropriate redox potential of BPA, the method exhibits high sensitivity and selectivity for BPA detection with a board linear range from 10−9 to10−5 M and a low detection limit of 1 fM. Moreover, the PEC method was applied to determine BPA in the real sample, finding the content of BPA in the cap of the infant feeding bottle is higher than that in the body.

A Very Simple Method for Detection of Bisphenol A in Environmental Water by Heme Signal Amplification

The detection of bisphenol A (BPA) was carried out using several simplified electrochemical sensors in which electroactive materials Heme, dopamine (DA), β-cyclodextrins (β-CD) or Fc-ECG were deposited on screen-printed electrodes (SPEs) through one-step. The preparation of the developed sensors were verified by SEM, CV and DPV. Results shown that Heme/SPE sensor demonstrated notable electro-catalytic capabilities and a greater current response than other sensors for BPA detection and optimal operating conditions were determined. The current response vs concentration of BPA showed a linear relationship in the range of 0.05–0.50 μmol·l−1, the concentration limit of detection (S/N = 3) as 5.0 nmol·l−1 and 0.0013 μA·μM−1·cm−2 as the sensitivity of detection. There were no interferences arising from common elements that relate to BPA. Heme/SPE was applied for BPA detection in bottle drinking water, tap water and lake water; findings showed a total R.S.D. of less than 3.1% and average recovery from 95.0 to 104.0%. These results showed that the actual application of BPA detection was rapid and precise when placed in environmental water and even useful for safety verification.

MnO2 Nanowires-Decorated Reduced Graphene Oxide Modified Glassy Carbon Electrode for Sensitive Determination of Bisphenol A

In this paper, MnO2 nanowires-decorated reduced graphene oxide composite (MnO2 NWs-rGO) was synthesized by physical adsorption and controlled potential reduction. Means of X-ray diffraction (XRD), scanning electron microscopy (SEM), and cyclic voltammetry (CV) were used to characterize this material. Glassy carbon electrode (GCE) modified with MnO2 NWs-rGO composite was fabricated by drop-coating method for the sensitive determination of bisphenol A (BPA). Electrochemical behaviors of BPA at the MnO2 NWs-rGO/GCE were studied using CV and chronocoulometry (CC). Experimental results found that MnO2 NWs-rGO/GCE exhibited excellent electrocatalytic activity, sensitivity, stability, and selectivity towards BPA. Under the optimized experimental conditions, quantitative analysis was carried out using the second derivative linear sweep voltammetry (SDLSV), and the MnO2 NWs-rGO/GCE revealed a good linear response to BPA concentration ranging from 0.02–20 μM and 20–100 μM, and the detection limit was 6.0 nM (S/N = 3). This new method possessed some obvious advantages, such as high determination sensitivity, simple preparation process, rapid response and low cost. The sensing performance of MnO2 NWs-rGO/GCE was demonstrated for the detection of BPA in plastic samples, and satisfactory results have been obtained. Therefore, an efficient determination method for monitoring trace levels of BPA was developed.

Elaboration of an Imprinted Polymer Film Based on Chitosan Electrodeposition for the Voltammetric Detection of BPA

A voltammetric sensor for bisphenol A (BPA) detection was developed. It was based on a gold electrode modified by an electrodeposited molecularly imprinted chitosan (CS) film. Fourier Transformed Infrared spectroscopy, scanning electron microscopy and electrochemistry were used to characterize and compare the surface of the molecularly imprinted polymer (MIP) and non-molecularly imprinted polymer (NIP) films. The analytical performance of this biosensor was then established using square wave voltammetry (SWV) of the ferro/ferricyanide redox probe, in the range of −300 and 600 mV. The oxidation peak current was proportional to the logarithm of BPA concentration in the range between 10−3 M to 10−21 M with a limit of detection of 0.67 × 10−21 M. The elaborated sensor showed good repeatability (RSD 2.08%), reusability and recognition of BPA in the presence of similar structural moieties. The proposed sensors were successfully employed to determine BPA in real plastic bottles for drinking water.

3D paper-based microfluidic device: a novel dual-detection platform of bisphenol A.

A novel platform of microfluidic paper-based analytical devices (μPADs) for dual detection of bisphenol A (BPA), a model analyte, was fabricated using an electronic digital plotter to create the stacked layer of μPADs and generate the lateral-flow channel without using an external pump. Two detection techniques, including electrochemical detection and laser desorption ionization mass spectrometric detection (LDI-MS), were used complementarily to improve the precision in the detection of BPA. The fluid sample was delivered to both detection zones by the capillary action, automatically generated from the fabricated microfluidic device. For an electrochemical sensor, two ballpoint pens filled with silver nanoparticles (AgNPs) and multiwall carbon nanotube (MWCNT) ink were used to print onto the paper with a contact printing method using a digital plotter. To further improve the sensitivity, zinc oxide (ZnO) was used to modify both electrochemical and LDI-MS detection zones. For BPA detection, high electrocatalytic properties and strong UV absorption of ZnO promote the electron transfer in the electrochemical sensor and ionization efficiency in LDI-MS with low interferences compared with a conventional organic matrix. Under optimal conditions, this platform showed a dual detection capability for BPA with a detection limit of 0.35 μM for electrochemical detection and with an ultralow detection limit of 0.01 pM for LDI-MS. This novel platform might be very useful for trace analyses requiring high precision detection of various analytes.

Facet-energy inspired metal oxide extended hexapods decorated with graphene quantum dots: sensitive detection of bisphenol A in live cells.

The development of crystal-facet metal oxide heterostructures has been of great interest owing to their rational design and multifunctional properties at the nanoscale level. Herein, we report a facile solution-based method for the synthesis of single-crystal Cu2O nanostructures (i.e. Cu2O-CuO) as a core. Graphene quantum dots (GQDs) with varying concentrations are fabricated on the surface of Cu2O extended hexapods (EHPs) in ethanol solution at room temperature via self-assembly, where copper acts as a sacrificial model and a stabilizer as well. The Cu2O crystals displayed a good sensing activity toward BPA oxidation owing to their high energy facets, dangling bonds and great proportion of surface copper atoms. Structural, morphological, chemical and vibrational investigations were performed in detail, presenting high crystallinity of hybrid nanocomposites and Cu2O-CuO heterojunction positions along with the growth of GQDs on the core of Cu2O-CuO crystals. The electrochemical sensing performance of the as-fabricated Cu2O-CuO@GQD EHPs was monitored for the determination of bisphenol A (BPA) as an early diagnostic marker and environmental contaminant. The synergistic effects of the boosted surface area, exposed Cu {111} crystallographic planes and mixed copper valences enhance redox reaction kinetics by increasing the electron shuttling rate at the electrode-analyte junction. Benefitting from the improved electrocatalytic activity for BPA oxidation, the electrochemical sensor displayed the lowest limit of detection (≤1 nM), good chemical stability, a broad linear range (2 nM-11 mM), and high sensitivity (636 μA mM-1 cm-2). The Cu2O-CuO@GQD EHP-based sensing platform was used for BPA detection in water and human serum samples. We have also constructed a pioneering electrochemical sensing platform for BPA detection in live cells, which might be used as a marker for early disease diagnosis.

Rapid and Sensitive Detection of Bisphenol A Based on Self-Assembly.

Bisphenol A (BPA) is an endocrine disruptor that may lead to reproductive disorder, heart disease, and diabetes. Infants and young children are likely to be vulnerable to the effects of BPA. At present, the detection methods of BPA are complicated to operate and require expensive instruments. Therefore, it is quite vital to develop a simple, rapid, and highly sensitive method to detect BPA in different samples. In this study, we have designed a rapid and highly sensitive biosensor based on an effective self-assembled monolayer (SAM) and alternating current (AC) electrokinetics capacitive sensing method, which successfully detected BPA at nanomolar levels with only one minute. The developed biosensor demonstrates a detection of BPA ranging from 0.028 μg/mL to 280 μg/mL with a limit of detection (LOD) down to 0.028 μg/mL in the samples. The developed biosensor exhibited great potential as a portable BPA biosensor, and further development of this biosensor may also be useful in the detection of other small biochemical molecules.

Advanced sensing platform for electrochemical monitoring of the environmental toxin; bisphenol A.

Environmental xenoestrogen, Bisphenol A (BPA), is a vitally important industrial raw chemical which can bring about a wide variety of adverse impacts on our health and environment. Therefore, it is imperative to develop efficacious systems to measure BPA and improve the life quality. Herein, a mixture of titanium dioxide nanoparticles (TiO2NPs) and glutaraldehyde cross-linked chitosan (GA-CS) loaded into a carbon nanotubes paste matrix to construct a novel electrochemical sensor (TiO2NPs/GA-CS/CNTPE) with a synergetic intensified current signal for the quantitative analysis of BPA. The surface morphology of the modified sensor was assessed by field emission scanning electron microscope (FESEM), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). The prepared TiO2NPs/GA-CS/CNTPE displayed increased electrocatalytic activity toward BPA. The calibration curve of BPA shows linear response in the BPA concentration range of 0.01-6μM with the lowest limit of detection of 9.58nM (S/N=3). The sensor exhibited high sensitivity and selectivity, good reproducibility, desirable stability and excellent performance in detection of BPA in real samples, which prove that TiO2NPs/GA-CS/CNTPE is quite applicable for food, medical and environmental analyses.

Portable and automated fluorescence microarray biosensing platform for on-site parallel detection and early-warning of multiple pollutants

The portable and automated fluorescence microarray biosensing platform (FMB) that employed a compact hybrid optical structure, microfluidics, and microarray biosensors was constructed for on-site parallel detection of multiple analytes. In the FMB, a hybrid optical structure that composed of a 1 × 4 single mode fiber optic coupler, four fiber optic switches, a single-multi mode fiber optic bundle coupler was at the first time developed for the transmission of the excitation light and the collection and transmission of multi-channel fluorescence signals. Through the control of fiber optic switches, the parallel fluorescence assay of four channels could be achieved using only one excitation light and one photodiode detector on the basis of the time-resolved effect. This optical design not only greatly increased the efficiency of light transmission and fluorescence collection and detection sensitivity of the FMB, but also allows the miniaturization and portability of the whole system because of few optical separation elements used and no requirement of rigorous optical alignment. Taking Microcystin-LR (MC-LR), 2,4-D, atrazine (ATZ), and bisphenol A (BPA) for example, the application potential of the FMB to rapidly and parallelly detect four typical pollutants in real water with high sensitivity and specificity was demonstrated. The limits of detection of MC-LR, 2,4-D, ATZ, and BPA were 0.04 μg/L, 0.09 μg/L, 0.02 μg/L, and 0.03 μg/L, respectively. The FMB could also achieve early-warning of pollutants thanks to its ability of rapidity, high-frequency, and multiple-analyte detection. The FMB has significant implications as a multiplexable, portable, rapid, and quantitative detection platform for pollution accidents and water quality management to satisfy the increasing demands of alerting and protecting civilians.

Encapsulation of enzyme by metal-organic framework for single-enzymatic biofuel cell-based self-powered biosensor

Self-powered biosensor, as a new sensing platform, utilizes biofuel cells as synchronous power source and biosensor system, with the advantages of no external power source, simple instrumentation and easy miniaturization. Meanwhile, inspired by biomimics for the encapsulation of soft tissue within protective exteriors in nature, encapsulation of biological macromolecules by metal organic-frameworks (MOFs) have been proved to be an effective strategy for enhancing the biocatalytic activity in maintaining biological functions. Herein, we successfully encapsulated laccase (LAC) in zeolitic imidazolate framework-8 (ZIF-8) and combined it with bacterial cellulose (BC)/carboxylated multi-walled carbon nanotubes (c-MWCNTs) skeleton to prepare highly flexible BC/c-MWCNTs/ZIF-8@LAC electrode. Subsequently, we ingeniously designed an highly flexible self-powered sensing platform based on biofuel-cell driven by single-enzyme, harvesting bisphenol A (BPA) as a model analyte. The maximum power density (Pmax) of single-enzymatic biofuel cell (EBFC) exhibited an excellent linear dynamic range from 0.01 to 0.4 mM with a lower detection limit of 1.95 × 10−3 mM for BPA concentrations. Therefore, the designed single-EBFC-based self-powered sensor is feasible in BPA detection and has a broad application prospect in the field of water pollutants detection.

Activating ZnWO4 nanorods for efficient electroanalysis of bisphenol A via the strategy of In doping induced band gap change

The design and development of an ideal method for treating and monitoring endocrine disruptors have become one of the greatest challenges for solving the problems of environment and human health. In this study, different proportions of In were doped into ZnWO4 nanorods by hydrothermal methods. The effect of the doped In element on its structure, morphology, electrocatalytic performance was systematically studied. Modified carbon paste electrode by different ratios of In-doped ZnWO4 nanorods a simple, rapid and sensitive electrochemistry sensor for bisphenol A(BPA) was developed. The results show that the catalytic efficiency of In-doped ZnWO4 nanorods for BPA was 3.6 times higher than that of pure ZnWO4. The proposed sensor produced a wide linear range of 0.01–20 μM and a low detection limit (3.4 nM, S/N = 3) by differential pulse voltammograms measurements. Satisfactory repeatability, stability and anti-interference performance are also achieved on In-doped ZnWO4/CPE. Meanwhile, the proposed sensor realizes the detection of BPA spiked in local tap water samples successfully. Moreover, the possible mechanism of In-doped ZnWO4 nanorods for BPA catalysis and the feasibility of ZnWO4 doping modification were clarified, which provides a novel and effective strategy for the design and synthesis of high performance electrocatalysts.

An ultrasensitive fluorescent aptasensor based on truncated aptamer and AGET ATRP for the detection of bisphenol A.

Given the gigantic harmfulness of bisphenol A (BPA), a novel and ultrasensitive aptasensor, which employs the truncated BPA aptamer, click chemistry, and activators generated by electron transfer for atom transfer radical polymerization (AGET ATRP), was developed herein for the quantitative determination of BPA. Firstly, hairpin DNAs (hairpins) with a thiol at the 5' end and an azide group at the 3' end were conjugated with aminated magnetic beads (MBs) through heterobifunctional cross-linkers. BPA truncated aptamer (ssDNA-A) hybridizes with its complementary single-stranded DNA (ssDNA-B) to form double-stranded DNA. In the presence of BPA, ssDNA-A specifically captures BPA, and then ssDNA-B is released. Subsequently, the ssDNA-B hybridizes with hairpins to expose the azide group near the surface of the MBs. Then, propargyl-2-bromoisobutyrate (PBIB), the initiator of AGET ATRP containing alkynyl group, was conjugated with azide group of hairpins via the Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). Consequently, a large number of fluorescein-o-acrylate (FA) were introduced to the MBs through AGET ATRP, resulting in that the fluorescence intensity was increased dramatically. Obviously, the fluorescence intensity was especially sensitive to the change of BPA concentration, and this method can be used in quantitative determination of BPA. Under optimal conditions, a broad liner range from 100fM to 100nM and a low limit of detection (LOD) of 6.6fM were obtained. Moreover, the method exhibits not only excellent specificity for BPA detection over BPA analogues but high anti-interference ability in real water sample detection, indicating that it has huge application prospect in food safety and environment monitoring.

Integration of black phosphorus and hollow-core anti-resonant fiber enables two-order magnitude enhancement of sensitivity for bisphenol A detection

Hollow core anti-resonant fiber (HARF) has found a handful applications in optical communications, nonlinear optics and high power delivery. The intrinsic property of the fiber also renders it an ideal candidate for biosensing, which has not been explored intensively. Herein, we demonstrate an optical fiber sensing platform, taking advantages of the state-of-the-art HARF technology and superior physicochemical properties of 2D material black phosphorus, for ultra-sensitive detection of bisphenol A (BPA) in blood and environmental samples. The specially designed HARF can not only achieve broadband transmission of light, but also confine light in the low refractive-index liquid core, ensuring maximum overlap of light and liquid core. Modification of the inner surface of HARF with 2D black phosphorus nanoflakes functionalized with fluorescently labeled BPA-specific aptamer provides a smart sensing interface enabling highly selective detection of BPA via measuring the fluorescence. The limit of detection is 1.69pM, which is more than two orders of magnitude enhancement compared to the conventional plate assay. The proposed assay is not interfered with the BPA analogues BPB and BPS. The long optical path with tight optical confinement greatly enhances the analyte-light interaction and improves the sensitivity of the sensing platform. The proposed sensing platform can be further developed for versatile applications.

Plasmonic cellulose textile fiber from waste paper for BPA sensing by SERS.

Flexible plasmonic Surface-enhanced Raman scattering (SERS) substrates were fabricated using cellulose textile fibers, in which the textile fibers were recycled from waste paper in an eco-friendly way. The Glycidyltrimethylammonium chloride (GTAC) with positive charges was grafted onto the surface of the cellulose textile fibers through cationization. Plasmonic silver nanoparticles (Ag NPs) with negative charges were decorated onto the cellulose textile fibers via electrostatic interactions. After cationization, the variation range of the diameter of the cellulose textile fibers was significantly increased because part of the cellulose was dissolved under alkaline condition, leading to more 'hot spots' for SERS during the shrinking process. The cellulose textile fiber-Ag NPs nanocomposite was employed for monitoring bisphenol A (BPA) in water and soft drink by SERS and the sensitivity of BPA detection achieved 50 ppb. The recovery values of BPA in soda water samples were from 96% to 105%. These results illustrate that the cellulose textile fiber-Ag NPs nanocomposite can be used as flexible, high sensitivity SERS substrates for detecting harmful ingredients in food or environment.

Development of a Novel Tyrosinase Amperometric Biosensor Based on Tin Nanoparticles for the Detection of Bisphenol A (4.4-Isopropylidenediphenol) in Water

Highly crystalline poly-vinyl pyrrolidone (PVP) capped Sn nanocrystals with good size and shape uniformity was synthesized by a hydrothermal process. A highly sensitive amperometric biosensor for the detection of Bisphenol A (BPA) was developed by immobilizing Tyrosinase on to glassy carbon electrode (GCE) modified with Sn nanoparticles. The fabricated amperometric biosensor exhibited excellent electroactivity towards BPA oxidation catalysed by enzymatic reaction of tyrosinase together with good conductivity of Sn nanoparticles. The developed biosensor displayed linear range from 0.01 to 0.10 μmol L-1 and a detection limit (DL) of 1.8 nmol L-1 with a correlation coefficient of 0.989. Electrochemical impedance spectroscopy (EIS) obtained in buffer solution for Tyrosinase/SnNP/GCE had the lowest charge transfer resistance (Rct) value of 219 Ω, which indicated low charge transfer. There was an increase in Rct for Tyrosinase/GCE, SnNP/GCE and Bare GCE which was 316 Ω, 638 Ω and 598 Ω respectively. This indicated a strong resistance to charge transfer. It is reported for the first time the use of Sn nanoparticles modified on GCE and tyrosinase for detection of BPA.

Graphdiyne: A new promising member of 2D all-carbon nanomaterial as robust electrochemical enzyme biosensor platform

Graphdiyne (GDY), a new two-dimensional all-carbon allotrope composed of benzene rings and alkyne unites, was successfully synthesized via a crossing-coupling reaction with much intriguing properties especially highly π-conjugated structure, attractive electronic and chemical properties, good biocompatibility and dispersion in aqueous solution. The as-prepared graphdiyne was explored for the first time as an extremely attractive matrix for tyrosinase (a model enzyme) immobilization to construct a mediator-free GDY-based biosensor for rapid detection of bisphenol A (BPA). The response of GDY-based tyrosinase biosensor is linear over the range of 1.0 × 10−7 to 3.5 × 10−6 mol L−1 with a high sensitivity of 2990.8 mA cm−2 M−1 and a low detection limit of 24 nmol L−1. The proposed GDY-based tyrosinase biosensor exhibited better analytical performances for BPA detection than CNTs and graphene based biosensors. The excellent performance of the biosensor should partially be ascribed to the strong π-π interactions between graphdiyne and BPA, which could enrich available BPA concentration on the electrode to react with tyrosinase. The robust GDY-based tyrosinase biosensor was used for BPA detection in drinking bottles and tap water with satisfactory results. As a new 2D all-carbon nanomaterial, graphdiyne is proved to be a powerful electrochemical enzyme biosensor platform for biomolecules (as recognition elements) immobilization and biosensor fabrication, and provides great application prospect for biomedical detection and environmental analyses.