Abstract

Highly crystalline poly-vinyl pyrrolidone (PVP) capped Sn nanocrystals with good size and shape uniformity was synthesized by a hydrothermal process. A highly sensitive amperometric biosensor for the detection of Bisphenol A (BPA) was developed by immobilizing Tyrosinase on to glassy carbon electrode (GCE) modified with Sn nanoparticles. The fabricated amperometric biosensor exhibited excellent electroactivity towards BPA oxidation catalysed by enzymatic reaction of tyrosinase together with good conductivity of Sn nanoparticles. The developed biosensor displayed linear range from 0.01 to 0.10 μmol L-1 and a detection limit (DL) of 1.8 nmol L-1 with a correlation coefficient of 0.989. Electrochemical impedance spectroscopy (EIS) obtained in buffer solution for Tyrosinase/SnNP/GCE had the lowest charge transfer resistance (Rct) value of 219 Ω, which indicated low charge transfer. There was an increase in Rct for Tyrosinase/GCE, SnNP/GCE and Bare GCE which was 316 Ω, 638 Ω and 598 Ω respectively. This indicated a strong resistance to charge transfer. It is reported for the first time the use of Sn nanoparticles modified on GCE and tyrosinase for detection of BPA.

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