Detection Of Glucose Concentration Research Articles

Investigation of a highly sensitive vortex beam-shined optical sensor for the detection of glucose concentration in urine

The present work investigates the notion of a highly sensitive optical sensor for the detection of urinary glucose concentration by shining an optical Vortex Beam. The proposed sensing scheme relies on the theory of multimode interference where the transmitted output power varies due to the change in the refractive index of cladding as formed by various concentrations of glucose in urine. With a significant amount of energy at the beam’s periphery, Vortex beam can energize a massive number of higher order modes inside the sensing system. In support of theoretical analysis, an Eigenmode expansion solver is employed in Lumerical Mode solution software to visualize the full transmission spectrum of the sensor. In contrast to the conventional sensor, the proposed sensor proffers a 3.65 times superior sensitivity of around 1.116 dB/gm/dL over the glucose concentration range from 0-10 gm/dL. The presence of minimum 0.008 gm/dL glucose level in urine could be detected using the proposed technique; whereas the traditional Gaussian-beam based technology could detect the presence of around 0.032 gm/dL of the same. Due to the high sensing performance, the present paradigm can be a useful tool for monitoring the elevated glucose levels which is responsible for triggering Diabetes Mellitus.

A method for identification of analyte concentration in aqueous solution by using a mistuned fluid-structure coupled vibration

In this paper, a method is presented for measurement of analyte concentration in an aqueous solution, using the magnitude change of frequency response induced by mistuned fluid-structure coupled vibration of the suggested structure immersed in the solution. Firstly, the operating principle is explained by analyzing natural characteristics and mistuned responses of the suggested structure by using finite element method (FEM). Then added mass effect induced by the fluid-structure interaction is formulated, by which variations of the analyte concentration can be manifested into the change of the added mass acting on the suggested structure. Based on the operating principle, the resonant amplitudes of the structure are investigated under different fluid densities, and it shows much higher sensitivity than the frequency changes used in previous works. Lastly, the suggested structure is optimized and simulated for detecting glucose concentration at human blood glucose level and noticeable differences are observed for different glucose concentrations.

Non-Invasive Measurement of Glucose Concentration Using Red and Near-Infrared Light-Emitting Diodes

Many diabetic mellitus patients envision a non-invasive method of blood glucose measurement since they require periodic monitoring of their blood glucose levels to ensure that it is stable and within the normal range. In this study, we detected glucose concentration using commercial light emitting diodes (LEDs) with a wavelength of 700–1600 nm. Light of long wavelengths (e.g. NIR) infiltrate human skin and reach the blood vessel lining, thereby aiding in the non-invasive measurement of blood glucose concentration. To demonstrate this, the concentration of glucose solutions was measured using red and NIR-LEDs in a non-invasive manner. The sensitivity of glucose detection was greater when light of wavelength below 1000 nm was used, owing to the absorption of wavelengths above 1000 nm by water. Furthermore, we controlled the input current of the red and NIR-LEDs to confirm the light intensity deviation with increasing glucose concentrations and suggested the optimum wavelength of light using this in-vitro system. Among various LEDs, the 700 nm LED showed higher light intensity deviation with change in injection current compared to LEDs with the other wavelengths. In particular, compared to other LEDs, a stark difference was observed in the light intensity of the 700 nm LED while measuring glucose concentrations in the range of 50–100 mg/dl.

Liquid photoacoustic sensing with high sensitivity by temperature compensated differential detection method

In this letter, a temperature compensated differential (TCD) photoacoustic (PA) method is proposed to simultaneously suppress the side-effect of multiple interferences, significantly improving the accuracy and sensitivity of liquid PA detection. We firstly established a theoretical model to prove the validity of this method. Then a differential PA detection system was set up to detect glucose concentration, a typical weak absorbing substance in the near-infrared range. The experimental results demonstrate that a trace-level detection limit of () for glucose solution was obtained. The TCD-PA method provides a feasible way for on-line high-precision detection of liquid substances.

A Nanoscale Structure Based on a Ring With Matchstick-Shape Cavity for Glucose Concentration and Temperature Detection

The design of micro-nano optical structure is the key problem in the improvement of integrated optical device. In this work, a compact nanosensor structure comprising metal-insulator-metal (MIM) waveguide and a ring with a matchstick-shape cavity (RMSC), is proposed and numerically simulated by using the finite element method. The transmission characteristics of this nanosensor structure are systematically analyzed. Moreover, the influences of the position of matchstick-shape structure inside the ring and the various structural parameters of RMSC on Fano resonance are discussed. The sensitivity and figure of merit (FOM) are used to describe the sensing performance. The maximum sensitivity can achieve 2162 nm/RIU with a FOM of 52.73. Furthermore, the applications of designed structure are investigated, which include biological detection and temperature sensing. The temperature sensitivity reaches as high as 1.525 nm/°C, and the best sensitivity of biosensor for detecting glucose concentration is 0.45 (nm · L)/g. The results pave a promising route toward ultra-compact plasmonic sensors for achieving high sensitivity and smaller scales, and applying in optical on-chip nanodevice in the future.

Micropillar based active microfluidic mixer for the detection of glucose concentration

Active microfluidic mixer has attracted remarkable interest towards the applications in the Lab on Chip (LoC) for fast and accurate fluid mixing. In this work, the standard MEMS processes have been successfully implemented to fabricate the active electromagnetic microfluidic mixer incorporating micropillar on flexible membrane for glucose measurement. Three main parts of the mixer, i.e., a planar electromagnetic coil with permanent magnet, a mechanical movable membrane with pillar and microfluidic channels that were fabricated separately and later bonded with corona discharging process to complete the construction of the microfluidic mixer. The mechanical movable membrane and microfluidic channels are made of polymer and fabricated using soft-lithography technique. Analysis results show that the fabricated microfluidic mixer operated using low electrical current of below 0.5 A produced the mixing index above 0.8 within 4 s. Mixture analysis of fluid samples tested at frequencies of 1.1 kHz, 2.3 kHz and 3.7 kHz shows much better mixing performance as compared to its passive mode condition. The functionality of the fabricated mixer was tested by mixing the DNS reagent and glucose solution. The detection of glucose concentration in the microfluidic mixer chip is indicated by comparing the correlation coefficient R2 with the conventional method. The evaluation result shows that the R2 of the mixture produced by the fabricated device shows a comparable value with that of conventional method showing a very promising approach for the diabetic patient diagnosis and food analysis.

Effect of sidt2 Gene on Cell Insulin Resistance and Its Molecular Mechanism.

Background Sidt2 (SID1 transmembrane family, member 2) is a multiple transmembrane lysosomal membrane protein newly discovered in our previous study. In the previous study, we used gene targeting technique to make a mouse model of sidt2 gene knockout (sidt2−/−). It was found that sidt2−/− mice showed elevated fasting blood glucose and impaired glucose tolerance, showing a disorder of glucose metabolism, suggesting that sidt2 may be closely related to insulin resistance. We used 3T3-L1 adipocytes, C2-C12 myoblasts, and HEPA1-6 hepatoma cells as subjects to observe the effects of sidt2 on insulin-stimulated glucose uptake and the abovementioned insulin signal transduction pathways, and then to explore the effect of sidt2 on peripheral tissue insulin resistance and its possible molecular mechanism. Methods (1) Lentiviruses with sidt2 gene knockout and puromycin resistance were constructed by Crispr/cas9 vector and transfected into 3T3-L1 adipocytes, C2-C12 myoblasts, and HEPA1-6 hepatoma cells to construct sidt2 knockout cell line model. (2) Glucose uptake of 3T3-L1 adipocytes, C2-C12 myoblasts, and HEPA1-6 hepatoma cells stimulated by insulin was detected by glucose detection kit, and the results were analyzed. (3) Sidt2 knockout group and control group of 3T3-L1 adipocytes, C2-C12 myoblast, and HEPA1-6 hepatoma cells were cultured according to the routine method. The total proteins of the above cells were extracted, and the expression of PAKT (thr308), PI3-K, and PIRS-1 (ser307) in the IRS-1 signaling pathway of the three groups was detected by western blot technique. Results (1) The sidt2 elimination models of 3T3-L1 adipocytes, C2-C12 myoblasts, and HEPA1-6 hepatoma cells were successfully constructed. (2) It was found that the glucose uptake of cells in the sidt2 knockout group was lower than that in normal group under insulin stimulation through the detection of glucose concentration in the cell culture medium. (3) It was found that the expression of PAKT (thr308) and PI3-K protein decreased and the expression of PIRS-1 (ser307) protein increased in sidt2−/− group compared to the control group. Conclusions sidt2 knockout can reduce glucose uptake in peripheral tissue under insulin stimulation, which may lead to peripheral tissue insulin resistance by affecting the IRS-1 signal pathway.

A Smartphone-Based Fluorescence Microscope With Hydraulically Driven Optofluidic Lens for Quantification of Glucose

The traditional method for early diagnosis of diabetes is to detect urine or blood glucose levels. However, the present clinical detection methods cause either cross-infection or require expensive instruments with complex procedures of operation. In this work, a handheld smartphone-based fluorescence microscope with the tunable optofluidic lens and microfluidic assay is demonstrated for the facile detection of glucose concentration for the first time to our knowledge. The tunability of the optofluidic lens is based on the dynamic deformation of an elastomeric membrane driven by adjustable hydraulic pressure. And the glucose sensor integrates a smartphone, a tunable optofluidic lens, a microfluidic chip, a customized holder and some easily accessible optics. A low detection limit of 0.33 mM of glucose was achieved subjected to a linear range of detection from 0 to 6 mM glucose, which is evaluated with an enzymatic fluoresce method. These results show that the cost-effective miniaturized system can be used for early screening and diagnosis of diabetes and potentially some other onsite applications of clinical diagnosis and environment monitoring.

A Novel Hybrid Plasmonic Resonator With High Quality Factor and Large Free Spectral Range

Whispering gallery (WG) mode resonators are experiencing a surge in the application of biosensing, attributing to high speed, flexibility, low cost, multi-analyte detection and compatibility with extra optical elements. The decoration of localized surface plasmon nanostructures to a dielectric WG mode resonator offers unprecedented sensitivity compared with a pure dielectric resonator. However, at the moment, the experimental quality (Q) factors of dielectric micro rings always achieve the value of 104 to 106, whereas the only reported hybrid counterpart possesses the Q factor of 300 and most studies are focused on the theoretical design. The resulting experimental detection limits (DLs) are in the range of 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-6</sup> 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-5</sup> RIU, which is one order of magnitude lower than the best value of hybrid resonators. The hybrid resonator should possess the experimental Q factor of 5000 to 10000 so as to match their purely dielectric rivals. We demonstrated a novel hybrid plasmonic WG mode resonator working on the multimode resonance mechanism. Under the condition of the multimode resonance, predominant field was distributed on the waveguide surface resulting in an improved bulk sensitivity. The multimode resonance promoted satisfied resonance frequency reduced and thus a high Q factor and a low value of DL could be achieved in the experiment. Afterwards, the resonator was made via micro-and nanofabrication. It was shown that the experimental Q factor of 5685 and the free spectral range larger than 35 nm, which were superior to the previously reported value to our best knowledge. Hypoglycemia and Hyperglycemia will produce noticeable organ damage over time. Early detection of glucose concentration is crucial for clinical diagnosis. The proposed resonator was used for the detection of glucose solution. The experimental sensitivity was 428 nm/RIU and the DL was 1.31 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-5</sup> RIU. The device with a footprint of 1500 μm × 12.7 μm, is able to integrate with other optical elements and developed as a commodity in the future.

Non-Invasive Fluidic Glucose Detection Based on Dual Microwave Complementary Split Ring Resonators With a Switching Circuit for Environmental Effect Elimination

In this paper, a microwave fluidic glucose sensor based on dual complementary split ring resonators (CSRRs) with a switching circuit is proposed for non-invasive and continuous glucose concentration detection. A CSRR has been designed to detect subtle changes in the dielectric constant of glucose solutions, and the electric field distribution is utilized to determine the effective sensing region. In a well-controlled environment, the glucose concentration can be detected by tracking the transmission coefficient of the designed CSRR. However, because the variations in dielectric constant with the glucose concentration are very small, the environmental factors (i.e., ambient temperature and relative humidity) can be dominant compared to the glucose concentration. To qualify the effect of the environmental factors, the variation in the transmission coefficient of the CSRR has been measured when the ambient temperature condition is varied from 293 K to 313 K and the relative humidity is varied from 30% to 90% inside temperature- and humidity-controlled chamber. Moreover, the environmental effects are calibrated out using two identical CSRRs with the switching circuit: one detects changes in the glucose concentration, while the other operates as a reference for environmental factors. After the elimination of the environmental effects, glucose concentrations in the range of 0 mg/dL to 400 mg/dL have been measured, and the sensitivity is 0.008dB/(100mg/dL). In addition, the reproducibility of the proposed sensor is verified by repeated measurements. It is demonstrated that the proposed sensor can detect glucose concentrations under practical environmental conditions.

Sensitivity enhancement of WS2-coated SPR-based optical fiber biosensor for detecting glucose concentration* *Project supported by the Natural Science Foundation of Jiangsu Province, China (Grant No. BK20171442), the China Postdoctoral Science Foundation (Grant No. 2018T110480), the Open Foundation of State Key Laboratory of Millimeter Waves, China (Grant No. K202003), the Open Foundation of State Key Laboratory of Luminescent Materials and Devices,

In this paper, we propose a theoretical model of the surface plasmon resonance-based optical fiber biosensor for detecting glucose concentration. The Au/ZnO/WS2 multilayer film is coated around optical fiber. Compared with the conventional surface plasmon resonance sensor, WS2 material can increase the sensitivity of the biosensor. The absorption capacity of WS2 is used to load glucose oxidase by forming a sensitive area to recognize glucose. Refractive index of the solution is calculated and then the concentration of the glucose can be obtained by the correspondence between refractive index and glucose concentration. The highest sensitivity of the SPR biosensor with a structure of 40-nm Au/5-nm ZnO/14 layers of WS2 is 4310 nm/RIU. The proposed WS2-based SPR fiber biosensor has a unique effect on the detection of glucose concentration. It is expected to have potential applications in future medical blood glucose concentration detection.

A Novel Design and Analysis of High-Sensitivity Biosensor Based on Nano-Cavity for Detection of Blood Component, Diabetes, Cancer and Glucose Concentration

In this study, a novel design of an optical biosensor based on resonance cavities and with high-resolution detection is proposed in a two-domains photonic crystal (2DPhCs) with free spectral range of around FSR = 630 nm. This sensor analyzes the internal components of the blood. It also detects a cancer patient from an ordinary person in the resonant wavelength range of 1.19760511 ~ 1.19760519 μm. Besides, it has the ability to detect the presence or absence of diabetes through human tear fluid with resonant wavelengths of 1.19760511 ~ 1.19760525 μm and measures the glucose concentration in urine. An analysis has been performed to determine the concentration of glucose in the normal state urine (0-15 mg/dL), urine with 0.625, 1.25, 2.5, 5 and 10 gm/dL of glucose in the wavelength range of 1.19760504 ~ 1.19760510 μm. Detection range is 0.2 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-7</sup> RIU. The primary function of the biosensor is the resonant cavity. The average bandwidth value of the output resonant wavelength is at least equal to 0.195 nm. The quality factor of the proposed sensor at worst is 6141.56. The percentage of transmission power is between 90 % and 100 %. The total area of the biosensor is 100 μm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . The sensitivity range is between 1250 nm/RIU and 10000 nm/RIU. The Figure of Merit (FOM) parameter at best is FOM = 48543 ± 118 RIU <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> . Detections of the disease through three methods (blood, urine, and tears fluid samples) is an important advantage of the proposed structure.

In-situ dual-channel surface plasmon resonance fiber sensor for temperature-compensated detection of glucose concentration.

External temperature variations inevitably affect the accuracy of surface plasmon resonance (SPR) biosensors. To that end, we propose an ultra-compact label-free dual-channel SPR fiber sensor (DSPRFS) that can simultaneously measure the glucose concentration and ambient temperature in real-time. The proposed sensor is based on a unique dual-channel structure fabricated by etching a side-hole fiber (SHF), and has significantly higher spatial sensitivity than traditional SPR biosensors. After coating with silver and zinc oxide films, one channel was filled with polydimethylsiloxane (PDMS) to sense the ambient temperature, and the other channel was immobilized with glucose oxidase (GOx) enzyme for glucose sensing. The proposed sensor is analyzed theoretically, fabricated and characterized. Glucose concentration sensitivity and temperature sensitivity of the manufactured sensor sample were found to be as high as 6.156 nm/mMand -1.604 nm/°C with limits of detection (LOD) of 16.24 µM and 0.06 °C, respectively. The proposed sensor has an extremely compact structure, enables temperature compensation, and is suitable for in-situ monitoring and high-precision sensing of glucose and other biological analytes.

Hybrid cellulose nanocrystal/magnetite glucose biosensors

There exists a high demand for simple and affordable blood glucose monitoring methods. For this purpose, new generations of biosensors are being developed for possible in vivo or dermal use. We present (non)sulphated cellulose nanocrystal/magnetite thin films to act as dermal and oral glucose biosensors. The biocompatible (N-CNC)-Fe3O4 and (S-CNC)-Fe3O4 hybrid systems exhibit peroxidase-like activity, indicated by an almost instant color change when in the presence of glucose and ABTS. Both types of biosensors detect glucose concentrations as low as 5 mM (which corresponds to the level of glucose in biological fluids), with (S-CNC)-Fe3O4 being 1.5 - 2 times as sensitive as (N-CNC)-Fe3O4. Hybrid catalytic activity is more pronounced at room temperature and in acidic environments. The hybrids can therefore be used to determine glucose levels by using sweat and saliva – non-blood bodily secretions which tend to be slightly to moderately acidic and have relatively low glucose levels.

A Glucose Concentration Detector With Wide-Range Converter Used on Livestock

In this study, a glucose concentration detector was used to detect the glucose concentration in the drinking solution of livestock for the first time. The detector comprised a glucose sensor and a proposed wide-range glucose concentration converter. The proposed wide-range glucose concentration converter can linearly convert the concentration of glucose into a duty cycle. To allow them to be used in various glucose sensors, scaling circuits were added to the glucose concentration detector to enable adaptation to the currents generated by glucose sensors. All functions of the glucose sensor and proposed wide-range glucose concentration converter were proved by conducting measurements. The measured input current range of the proposed converter was 30 nA to 3 mA. This ensured that a wide dynamic range of input currents of 100 dB was guaranteed. A glucose concentration in the range of 5%–30% could be detected, and the corresponding duty cycle of the proposed detector was in the range of 4.67%–89.5%. The maximum linear error was −0.75%. The sensitivity of the proposed detector was 3.39%/%, and the chip size was 1.13 $\times$ 1.13 mm2. Finally, an experiment to detect the glucose concentration in the drinking solution of livestock was conducted. The results revealed that the proposed converter was suitable for glucose concentration detection.

Automated Electrochemical Glucose Biosensor Platform as an Efficient Tool Toward On-Line Fermentation Monitoring: Novel Application Approaches and Insights.

Monitoring and control of fermentation processes remain a crucial challenge for both laboratory and industrial-scale experiments. Reliable identification and quantification of the key process parameters in on-line mode allow operation of the fermentation at optimal reactor efficiency, maximizing productivity while minimizing waste. However, state-of-the-art fermentation on-line monitoring is still limited to a number of standard measurements such as pH, temperature and dissolved oxygen, as well as off-gas analysis as an advanced possibility. Despite the availability of commercial biosensor-based platforms that have been established for continuous monitoring of glucose and various biological variables within healthcare, on-line glucose quantification in fermentation processes has not been implemented yet to a large degree. For the first time, this work presents a complete study of a commercial flow-through-cell with integrated electrochemical glucose biosensors (1st generation) applied in different media, and importantly, at- and on-line during a yeast fed-batch fermentation process. Remarkably, the glucose biosensor–based platform combined with the developed methodology was able to detect glucose concentrations up to 150 mM in the complex fermentation broth, on both cell-free and cell-containing samples, when not compromised by oxygen limitations. This is four to six-fold higher than previously described in the literature presenting the application of biosensors predominately toward cell-free fermentation samples. The automated biosensor platform allowed reliable glucose quantification in a significantly less resource and time (<5 min) consuming manner compared to conventional HPLC analysis with a refractive index (RI) detector performed as reference measurement. Moreover, the presented biosensor platform demonstrated outstanding mechanical stability in direct contact with the fermentation medium and accurate glucose quantification in the presence of various electroactive species. Coupled with the developed methodology it can be readily considered as a simple, robust, accurate and inexpensive tool for real-time glucose monitoring in fermentation processes.

Ni Hierarchical Structures Supported on Titania Nanowire Arrays as Efficient Nonenzymatic Glucose Sensor.

Developing new advanced nonenzymatic electrochemical nano-sensors for glucose detection has attracted intensive attraction. In this work, we designed a novel nanocomposite nonenzymatic glucose sensor by fabricating hierarchically nanostructured metal nickel on titania nanowire arrays, which was loaded on a transparent conductive substrate (i.e., fluorine-doped tin oxide, FTO) surface by mild hydrothermal method. Due to the large surface area of the hierarchically nanostructured Ni and fast electron transfer of the TiO₂ nanowire arrays electrode, the nanocomposite electrode shows excellent electrochemical activity toward the oxidation of glucose. The electrode exhibits high sensitivity in detecting glucose concentration (1472 μA mM-1 cm-2) with a wide linear range from 2×10-4 M to 2×10-3 M, fast response time (within 5 s), and small detection limit (10 μM) (S/N = 3). The good analytical performance, low cost and simple preparation method make this novel electrode material promising for the development of effective glucose nonenzymatic glucose sensor.

All-Inkjet-Printed Flexible Nanobio-Devices with Efficient Electrochemical Coupling Using Amphiphilic Biomaterials.

Nanostructured flexible electrodes with biological compatibility and intimate electrochemical coupling provide attractive solutions for various emerging bioelectronics and biosensor applications. Here, we develop all-inkjet-printed flexible nanobio-devices with excellent electrochemical coupling by employing amphiphilic biomaterial, an M13 phage, numerical simulation of single-drop formulation, and rational formulations of nanobio-ink. Inkjet-printed nanonetwork-structured electrodes of single-walled carbon nanotubes and M13 phage show efficient electrochemical coupling and hydrostability. Additive printing of the nanobio-inks also allows for systematic control of the physical and chemical properties of patterned electrodes and devices. All-inkjet-printed electrochemical field-effect transistors successfully exhibit pH-sensitive electrical current modulation. Moreover, all-inkjet-printed electrochemical biosensors fabricated via sequential inkjet-printing of the nanobio-ink, electrolytes, and enzyme solutions enable direct electrical coupling within the printed electrodes and detect glucose concentrations at as low as 20 μM. Glucose levels in sweat are successfully measured, and the change in sweat glucose levels is shown to be highly correlated with blood glucose levels. Synergistic combination of additive fabrication by inkjet-printing with directed assembly of nanostructured electrodes by functional biomaterials could provide an efficient means of developing bioelectronic devices for personalized medicine, digital healthcare, and emerging biomimetic devices.

Development of Highly Sensitive Ag NPs Decorated Graphene FET Sensor for Detection of Glucose Concentration

In this research work, non-enzymatic FET sensor using silver nanoparticles (Ag NPs) decorated graphene has been fabricated for the detection of various glucose concentration levels. Graphene was deposited using chemical vapour deposition technique and Ag NPs was decorated on the surface of graphene via sputtering technique. FET glucose sensor was tested under optimal conditions (pH 7.4) in linear range 0.1 µM–0.25 µM of glucose concentration levels. It was observed that, developed FET glucose sensor delivers high sensitivity which was compared with previously reported electrodes. The reproducibility of FET based glucose sensor was tested using different interfering species along with glucose concentrations. On examining the behavioural characteristics of FET sensor towards glucose detection it was found to offer a potential device for further real-time clinical applications.

Polyethyleneimine-Stabilized Platinum Nanoparticles as Peroxidase Mimic for Colorimetric Detection of Glucose.

Colorimetric detection of glucose using enzyme-mimic nanoparticles (NPs) has been drawing great attention. However, many NPs lack good stability in solution, which results in reduced color change of substrates in colorimetric detection. Liner soluble macromolecules with high cationic density may be suitable candidates for the stabilization of NPs. Herein, we prepared polyethyleneimine-stabilized platinum NPs (Ptn-PEI NPs) for colorimetric detection of glucose. The platinum NPs (Pt NPs) used in this system had small size (from 3.21 to 3.70 nm) and narrow size distribution. Pt50-PEI NPs had high stability within one week with a hydrodynamic size of ∼25 nm and slightly positive zeta potential. Pt50-PEI NPs-catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2, generating blue oxidized TMB (oxTMB), which indicated the peroxidase-like property of Pt50-PEI NPs. The optimal condition for this reaction was pH = 4.0 at 30 °C. More importantly, Pt50-PEI NPs were successfully used to detect glucose concentration by a colorimetric method with high selectivity. The established method had a linear concentration range from 10 to 5000 μM with a detection limit of 4.2 μM. For example, the concentration of glucose in saliva was tested to be 0.15 mM using our method. The high stability of Pt50-PEI NPs enhanced the high accessibility of the active center of Pt NPs for substrates and consequent excellent catalytic property. This established method has great potential to be used in various applications for glucose detection in the future.