Detachment Events Research Articles

Adhesion Mechanics and Detachment Dynamics of Vanishing Surface Layers on Hydrogels.

Cross-linked hydrogel surfaces exhibit reduced stiffness when polymerized against polymeric hydrophobic surfaces. As such, these layers play a critical role in contact mechanics, particularly exhibiting strong relative adhesion with colloidal probes when the contact area is small. This prevents the use of continuum models of adhesive soft contact. To connect mechanisms of stretch to the force response, depth-controlled nanoindentation experiments were conducted on polyacrylamide (pAAM) hydrogel samples using colloidal probe atomic force microscopy (AFM). The pAAM sample had a high water content of >90% and was molded against polyoxymethylene (POM) to create a more dilute surface layer with thickness ∼0.5 μm. Indentations to multiple depths between 50 nm and 1.25 μm were repeated 10 times each. First, the force drops during the unloading, and separation segments of each indentation were characterized. This described the detachment progression for increasing areas of contact, revealing that the pull-off force for a single chain was in the single-pN range. Second, the stretched polymer network was modeled as an array of parallel, linear springs. Assuming a constant areal chain density of α = 100 chains/μm2, the maximum force of adhesion was plotted versus the volume of chains stretched upward, and the average chain stiffness was calculated from a linear fit to be 22.8 × 10-6 N/m. A Weibull distribution analysis of detachment events revealed a dependence of chain stiffness on maximum indentation depth (dmax), with higher stiffness at shallower depths approaching kchain ≈ 20 × 10-6 N/m. These findings on adhesion mechanics between a vanishing hydrogel surface and probe can guide the development of multifunctional hydrogels for various biomedical applications.

Dynamic and large field of view photonic resonator absorption microscopy for ultrasensitive digital resolution detection of nucleic acid and protein biomarkers

In this paper, we describe a biosensing instrument based on our previously developed photonic resonator absorption microscope (PRAM) that incorporates autofocus, digital representation of the gold nanoparticle (AuNP) accumulation, and the ability to gather time-series image sequences of AuNP attachment and detachment from the photonic crystal (PC) surface. The combined capabilities are used to fully automate PRAM image collection during biomolecular assays to enable tiling of PRAM images to provide millimeter-scale field of view. The instrument can also gather PRAM “movies” that enables digital showcasing and dynamic counting AuNPs as they arrive and depart from the PC surface. We utilize the capabilities in the context of two biomolecular assays for detection of protein biomarkers in a conventional AuNP-tagged sandwich format. Utilizing dynamic counting of AuNP attachment and detachment events during the assay we present a detection for microRNA-375 (miRNA-375) down to 1 aM with a 10-min, room temperature, enzyme-free approach, while revealing characteristics of the binding-rate and unbinding-rate of the biomolecular interactions. Our instrument can potentially find broad applications in multiplexed point-of-care diagnostic testing, and as a general-purpose tool for quantitative characterization of biomolecular binding kinetics with single-molecule resolution.

A Semi-Markov Approach to Study a Group of Kinesin Motors.

We propose a general mathematical and computational approach to study cellular transport driven by a group of kinesin motors. It is a framework for multi-scale modeling that integrates kinetic models of single kinesin motors, including detachment and reattachment events, to study group behaviors of several motors. By formulating the problem as a semi-Markov process and applying a central limit theorem, asymptotic velocity and diffusivity can be readily calculated, which offers considerable computational advantage over Monte Carlo simulations in tasks such as parameter sensitivity analysis and model selection. We demonstrate the method with some examples. The importance of incorporating the intrinsic microscopic-level dynamics of individual motors is illustrated by showing how changes at the microscopic level propagate to the motor-cargo complex at a mesoscopic level. Particularly, we showcase an example in which changes in the second moment of single-motor characteristics gives rise to different first moment characteristics of the motor group.

Wetting and strain engineering of 2D materials on nanopatterned substrates.

The fascinating realm of strain engineering and wetting transitions in two-dimensional (2D) materials takes place when placed on a two-dimensional array of nanopillars or one-dimensional rectangular grated substrates. Our investigation encompasses a diverse set of atomically thin 2D materials, including transition metal dichalcogenides, hexagonal boron nitride, and graphene, with a keen focus on the impact of van der Waals adhesion energies to the substrate on the wetting/dewetting behavior on nanopatterned substrates. We find a critical aspect ratio of the nanopillar or grating heights to the period of the pattern when the wetting/dewetting transition occurs. Furthermore, energy hysteresis analysis reveals dynamic detachment and re-engagement events during height adjustments, shedding light on energy barriers of 2D monolayer transferred on patterned substrates. Our findings offer avenues for strain engineering in 2D materials, leading to promising prospects for future technological applications.

A versatile micromodel technology to explore biofilm development in porous media flows.

Bacterial biofilms that grow in porous media are critical to ecosystem processes and applications ranging from soil bioremediation to bioreactors for treating wastewater or producing value-added products. However, understanding and engineering the complex phenomena that drive the development of biofilms in such systems remains a challenge. Here we present a novel micromodel technology to explore bacterial biofilm development in porous media flows. The technology consists of a set of modules that can be combined as required for any given experiment and conveniently tuned for specific requirements. The core module is a 3D-printed micromodel where biofilm is grown into a perfusable porous substrate. High-precision additive manufacturing, in particular stereolithography, is used to fabricate porous scaffolds with precisely controlled architectures integrating flow channels with diameters down to several hundreds of micrometers. The system is instrumented with: ultraviolet-C light-emitting diodes; on-line measurements of oxygen consumption and pressure drop across the porous medium; camera and spectrophotometric cells for the detection of biofilm detachment events at the outlet. We demonstrate how this technology can be used to study the development of Pseudomonas aeruginosa biofilm for several days within a network of flow channels. We find complex dynamics whereby oxygen consumption reaches a steady-state but not the pressure drop, which instead features a permanent regime with large fluctuations. We further use X-ray computed microtomography to image the spatial distribution of biofilms and computational fluid dynamics to link biofilm development with local flow properties. By combining the advantages of additive manufacturing for the creation of reproducible 3D porous microarchitectures with the flow control and instrumentation accuracy of microfluidics, our system provides a platform to study the dynamics of biofilm development in 3D porous media and to rapidly test new concepts in process engineering.

Vesicles driven by dynein and kinesin exhibit directional reversals without regulators

Intracellular vesicular transport along cytoskeletal filaments ensures targeted cargo delivery. Such transport is rarely unidirectional but rather bidirectional, with frequent directional reversals owing to the simultaneous presence of opposite-polarity motors. So far, it has been unclear whether such complex motility pattern results from the sole mechanical interplay between opposite-polarity motors or requires regulators. Here, we demonstrate that a minimal system, comprising purified Dynein-Dynactin-BICD2 (DDB) and kinesin-3 (KIF16B) attached to large unilamellar vesicles, faithfully reproduces in vivo cargo motility, including runs, pauses, and reversals. Remarkably, opposing motors do not affect vesicle velocity during runs. Our computational model reveals that the engagement of a small number of motors is pivotal for transitioning between runs and pauses. Taken together, our results suggest that motors bound to vesicular cargo transiently engage in a tug-of-war during pauses. Subsequently, stochastic motor attachment and detachment events can lead to directional reversals without the need for regulators.

Simulations suggest robust microtubule attachment of kinesin and dynein in antagonistic pairs

Intracellular transport is propelled by kinesin and cytoplasmic dynein motors that carry membrane-bound vesicles and organelles bidirectionally along microtubule tracks. Much is known about these motors at the molecular scale, but many questions remain regarding how kinesin and dynein cooperate and compete during bidirectional cargo transport at the cellular level. The goal of the present study was to use a stochastic stepping model constructed by using published load-dependent properties of kinesin-1 and dynein-dynactin-BicD2 (DDB) to identify specific motor properties that determine the speed, directionality, and transport dynamics of a cargo carried by one kinesin and one dynein motor. Model performance was evaluated by comparing simulations to recently published experiments of kinesin-DDB pairs connected by complementary oligonucleotide linkers. Plotting the instantaneous velocity distributions from kinesin-DDB experiments revealed a single peak centered around zero velocity. In contrast, velocity distributions from simulations displayed a central peak around 100 nm/s, along with two side peaks corresponding to the unloaded kinesin and DDB velocities. We hypothesized that frequent motor detachment events and relatively slow motor reattachment rates resulted in periods in which only one motor is attached. To investigate this hypothesis, we varied specific model parameters and compared the resulting instantaneous velocity distributions, and we confirmed this systematic investigation using a machine-learning approach that minimized the residual sum of squares between the experimental and simulation velocity distributions. The experimental data were best recapitulated by a model in which the kinesin and dynein stall forces are matched, the motor detachment rates are independent of load, and the kinesin-1 reattachment rate is 50 s−1. These results provide new insights into motor dynamics during bidirectional transport and put forth hypotheses that can be tested by future experiments.

Robust microtubule dynamics facilitate low-tension kinetochore detachment in metaphase.

During mitosis, sister chromatids are stretched apart at their centromeres via their attachment to oppositely oriented kinetochore microtubules. This stretching generates inwardly directed tension across the separated sister centromeres. The cell leverages this tension signal to detect and then correct potential errors in chromosome segregation, via a mechanical tension signaling pathway that detaches improperly attached kinetochores from their microtubules. However, the sequence of events leading up to these detachment events remains unknown. In this study, we used microfluidics to sustain and observe low-tension budding yeast metaphase spindles over multiple hours, allowing us to elucidate the tension history prior to a detachment event. We found that, under conditions in which kinetochore phosphorylation weakens low-tension kinetochore-microtubule connections, the mechanical forces produced via the dynamic growth and shortening of microtubules is required to efficiently facilitate detachment events. Our findings underscore the critical role of robust kinetochore microtubule dynamics in ensuring the fidelity of chromosome segregation during mitosis.

A microscale system for in situ investigation of immobilized microalgal cell resistance against liquid flow in the early inoculation stage.

In attached microalgae cultivation systems, cell detachment due to fluid hydrodynamic flow is not a subject matter that is commonly looked into. However, this phenomenon is of great relevance to optimizing the operating parameters of algae cultivation and feasible reactor design. Hence, this current work miniaturizes traditional benchtop assays into a microfluidic platform to study the cell detachment of green microalgae, Chlorella vulgaris, from porous substrates during its early cultivation stage under precisely controlled conditions. As revealed by time lapse microscopy, an increase in bulk flow velocity facilitated nutrient transport but also triggered cell detachment events. At a flow rate of 1000 μL min-1 of growth medium for 120 min, the algal cell coverage was up to 5% lower than those at 5 μL min-1 and 50 μL min-1. In static seeding, the evolution of attached cell resistance toward liquid flows was dependent on hydrodynamic zones. The center zone of the microchannel was shown to be a "comfortable zone" of the attached cells to sequester nutrients effectively at lower medium flow rates but there was a profile transition where outlet zones favored cell attachment the most at higher flow rates (1.13 times higher than the center zone for 1000 μL min-1). Besides, computational fluid dynamics (CFD) simulations illustrated that the focusing band varied between cross-sections and depths, while the streamline was the least concentrated along the side walls and bottom plane of the microfluidic devices. It was intriguing to learn that cell detachment was not primarily happening along the symmetry streamline. Insight gained from this study could be further applied in the optimization of operating conditions of attached cultivation systems whilst preserving laminar flow conditions.

Adhesion strength and anti-tumor agents regulate vinculin of breast cancer cells.

The onset and progression of cancer are strongly associated with the dissipation of adhesion forces between cancer cells, thus facilitating their incessant attachment and detachment from the extracellular matrix (ECM) to move toward metastasis. During this process, cancer cells undergo mechanical stresses and respond to these stresses with membrane deformation while inducing protrusions to invade the surrounding tissues. Cellular response to mechanical forces is inherently related to the reorganization of the cytoskeleton, the dissipation of cell–cell junctions, and the adhesion to the surrounding ECM. Moreover, the role of focal adhesion proteins, and particularly the role of vinculin in cell attachment and detachment during migration, is critical, indicating the tight cell–ECM junctions, which favor or inhibit the metastatic cascade. The biomechanical analysis of these sequences of events may elucidate the tumor progression and the potential of cancer cells for migration and metastasis. In this work, we focused on the evaluation of the spreading rate and the estimation of the adhesion strength between breast cancer cells and ECM prior to and post-treatment with anti-tumor agents. Specifically, different tamoxifen concentrations were used for ER+ breast cancer cells, while even concentrations of trastuzumab and pertuzumab were used for HER2+ cells. Analysis of cell stiffness indicated an increased elastic Young’s modulus post-treatment in both MCF-7 and SKBR-3 cells. The results showed that the post-treatment spreading rate was significantly decreased in both types of breast cancer, suggesting a lower metastatic potential. Additionally, treated cells required greater adhesion forces to detach from the ECM, thus preventing detachment events of cancer cells from the ECM, and therefore, the probability of cell motility, migration, and metastasis was confined. Furthermore, post-detachment and post-treatment vinculin levels were increased, indicating tighter cell–ECM junctions, hence limiting the probability of cell detachment and, therefore, cell motility and migration.

Correlative Analysis of Intra‐ Versus Extracellular Cell Detachment Events via the Alignment of Optical Imaging and Detachment Force Quantification

AbstractIn recent decades, hybrid characterization systems have become pillars in the study of cellular biomechanics. Especially, Atomic Force Microscopy (AFM) is combined with a variety of optical microscopy techniques to discover new aspects of cell adhesion. AFM, however, is limited to the early‐stage of cell adhesion, so that the forces of mature cell contacts cannot be addressed. Even though the invention of Fluidic Force Microscopy (FluidFM) overcomes these limitations by combining the precise force‐control of AFM with microfluidics, the correlative investigation of detachment forces arising from spread mammalian cells has been barely achieved. Here, a novel multifunctional device integrating Fluorescence Microscopy (FL) into FluidFM technology (FL‐FluidFM) is introduced, enabling real‐time optical tracking of entire cell detachment processes in parallel to the undisturbed acquisition of force‐distance curves. This setup, thus, allows for entailing two pieces of information at once. As proof‐of‐principle experiment, this method is applied to fluorescently labeled rat embryonic fibroblast (REF52) cells, demonstrating a precise matching between identified force‐jumps and visualized cellular unbinding steps. This study, thus, presents a novel characterization tool for the correlated evaluation of mature cell adhesion, which has great relevance, for instance, in the development of biomaterials or the fight against diseases such as cancer.

Modeling of the Particle Build-Up Evolution on a Single-Wire Magnetic Capture from Axial Stream Flow

The kinetic equation of the accumulation of magnetic particles from axial flow on a magnetized ferromagnetic wire in an external homogeneous magnetic field has been developed in this study. A new differential equation of the evolution of the accumulation radius over time, which considers both the capture and the detachment of the particles in the accumulation profile on the wire, has been formulated. The evolution of the radius of the accumulation profile over time was obtained from both the differential kinetic equation based on population theory and from the stochastic Fokker–Planck equation. In the limit approach (t→∞), it was observed that the expressions of the saturation radius of the accumulation radius on the magnetized wire of the particles obtained from both models were the same. It is emphasized that the obtained results are valid for both the initial and steady-state build-up of the particle capture process. These results were compared with the experimental results from the literature, and it was observed that the theoretical and experimental results were in good agreement. The effects of both capture and detachment events on the accumulation of particles on the magnetized wire were evaluated.

Perspective on statistical effects in the adhesion of micropatterned surfaces

Bioinspired micropatterned adhesives have attracted extensive research interest in the past two decades. In modeling the performance of these adhesives, the common assumption has been that the adhesive strength of each sub-contact is identical. Recent experiments, however, have shown that interfacial defects of different characters lead to a distribution of the adhesive strength within a fibrillar array. Based on experimental observations of detachment events, a statistical model for the distribution of the local adhesive strength and the resulting performance of a micropatterned adhesive are presented. This approach constitutes a paradigm shift, providing better understanding of micropatterned adhesives under real conditions. Examples presented include the prediction of unstable detachments in compliant systems. Future directions are discussed, including the extension of the statistical approach to non-uniform loading and rate-dependent effects, the contribution of suction to adhesion and aging of contacts over specific time periods, as well as the necessity for a more in-depth understanding of defect formation considering surface roughness and other imperfections in the system.

Safety and efficacy of nurse led intravitreal injection service with Precivia® injection assist device.

Intravitreal anti-VEGF injections are the most frequently performed outpatient procedure in the UK. Ophthalmic allied healthcare professionals are replacing medical professionals in delivering injections nationwide. The use of injection assist devices such as Precivia® has been well established and increasingly adopted to aid in their safe delivery. We present outcomes of nurse-led intravitreal injections using the Precivia® injection assist device over a five-year period in the UK. A retrospective review was completed of all anti-VEGF intravitreal injections delivered at the Great Western Hospital between May 2015 and May 2020. Over the five-year study period, 2318 patients underwent a total of 26,923 intravitreal injections; 20,421 (75.8%) of which were delivered by appropriately trained ophthalmic nurses. The annual number of injections increased year-on-year from 2112 injections in 2015-2016 to 5410 injections in 2019-2020. The mean age of patients was 75.7±12.2 years with a female-to-male ratio was 1.17:1. Wet age-related macular degeneration represented the major indication for injections followed by retinal vein occlusion and diabetic maculopathy respectively. Three cases of post-injection endophthalmitis out of 20,421 (0.015%) injections in nurse injection group were identified during the study period. There were no cases of lens touch, retinal detachment or systemic thromboembolic events. Use of the Precivia® intravitreal injection assist device by trained ophthalmic allied health professionals is a safe and cost-effective way to deliver intravitreal injections service.

Effect of COVID-19-related lockdown on ophthalmic practice in Italy: A report from 39 institutional centers.

To compare the number of eye surgical procedures performed in Italy in the 2 months following the beginning of lockdown (study period) because of COVID-19 epidemic with those performed in the two earlier months of the same year (intra-year control) and in the period of 2019 corresponding to the lockdown (inter-year control). Retrospective analysis of surgical procedures carried out at 39 Academic hospitals. A distinction was made between elective and urgent procedures. Intravitreal injections were also considered. Percentages for all surgical procedures and incidence rate ratios (IRR) for rhegmatogenous retinal detachment (RRD) events were calculated. A p value <0.05 was considered significant. A total of 20,886 versus 55,259 and 56,640 patients underwent surgery during the lockdown versus intra-and inter-year control periods, respectively. During the lockdown, only 70% of patients for whom an operation/intravitreal injection was recommended, finally underwent surgery; the remaining patients did not attend because afraid of getting infected at the hospital (23%), taking public transportation (6.5%), or unavailable swabs (0.5%). Elective surgeries were reduced by 96.2% and 96.4%, urgent surgeries by 49.7% and 50.2%, and intravitreal injections by 48.5% and 48.6% in the lockdown period in comparison to intra-year and inter-year control periods, respectively. IRRs for RRDs during lockdown dropped significantly in comparison with intra- and inter-year control periods (CI: 0.65-0.80 and 0.61-0.75, respectively, p < 0.001 for both). This study provides a quantitative analysis of the reduction of eye surgical procedures performed in Italy because of the COVID-19 epidemic.

A Review of Single-Cell Adhesion Force Kinetics and Applications.

Cells exert, sense, and respond to the different physical forces through diverse mechanisms and translating them into biochemical signals. The adhesion of cells is crucial in various developmental functions, such as to maintain tissue morphogenesis and homeostasis and activate critical signaling pathways regulating survival, migration, gene expression, and differentiation. More importantly, any mutations of adhesion receptors can lead to developmental disorders and diseases. Thus, it is essential to understand the regulation of cell adhesion during development and its contribution to various conditions with the help of quantitative methods. The techniques involved in offering different functionalities such as surface imaging to detect forces present at the cell-matrix and deliver quantitative parameters will help characterize the changes for various diseases. Here, we have briefly reviewed single-cell mechanical properties for mechanotransduction studies using standard and recently developed techniques. This is used to functionalize from the measurement of cellular deformability to the quantification of the interaction forces generated by a cell and exerted on its surroundings at single-cell with attachment and detachment events. The adhesive force measurement for single-cell microorganisms and single-molecules is emphasized as well. This focused review should be useful in laying out experiments which would bring the method to a broader range of research in the future.

Water Distribution in Fuel Cell Gas Channels Using a Mechanistic Discrete Particle Model

Water removal and accumulation in fuel cell gas channels is a complex phenomenon which is a consequence of the strength of the capillary forces and inertial forces (1 < Re < 1000). The presence of water in the channel restricts the access of oxygen to diffuse through the gas diffusion layers to the catalyst layer. The air supplied exchanges momentum to the droplets and water clusters, causing detachment when the adhesion force is overcome.Water emerging from discrete locations as droplets interact with both the channel walls and each discrete water cluster, depending on the configuration and operating conditions. Complexity increases as multiple detachment and coalescence events occur over large channel lengths and time scales. Modelling these systems with traditional method computational fluid dynamic (CFD) methods is computationally expensive so we have developed a new mechanistic discrete particle model to handle the liquid water two-phase flow.Taking inspiration from interface resolving volume of fluid (VoF) simulations of water flow in gas channels, we use analytically equations determined by the equilibrium contact angle in different configurations on the channel wall to predict detachment times and coalescence events. We compare the prediction of water area coverage ratio and channel saturation to the VoF simulations and can also estimate dynamic pressure drop.Using this model, we are able to investigate the dynamic effects of liquid water accumulation and distribution in the channel, with often periodic detachment events arising from the formation of liquid plugs. Consequently, the model is able to investigate the effect of the channel wall wettability, from hydrophilic to hydrophobic and the impact that has on the distribution and accumulation of water in the system.We compare the speed of this model against the VoF simulations to show that this type of modelling of two-phase flow in the channel can enable higher resolution accuracy when coupled with continuum scale approaches. Furthermore, with this approach it is possible to investigate the effect of: channel dimensions, liquid water flow rate and spatial location of liquid water emergence. This should allow for designed fuel cell structures to be evaluated. Figure 1

Effective behavior of cooperative and nonidentical molecular motors.

Analytical formulas for effective drift, diffusivity, run times, and run lengths are derived for an intracellular transport system consisting of a cargo attached to two cooperative but not identical molecular motors (for example, kinesin-1 and kinesin-2) which can each attach and detach from a microtubule. The dynamics of the motor and cargo in each phase are governed by stochastic differential equations, and the switching rates depend on the spatial configuration of the motor and cargo. This system is analyzed in a limit where the detached motors have faster dynamics than the cargo, which in turn has faster dynamics than the attached motors. The attachment and detachment rates are also taken to be slow relative to the spatial dynamics. Through an application of iterated stochastic averaging to this system, and the use of renewal-reward theory to stitch together the progress within each switching phase, we obtain explicit analytical expressions for the effective drift, diffusivity, and processivity of the motor-cargo system. Our approach accounts in particular for jumps in motor-cargo position that occur during attachment and detachment events, as the cargo tracking variable makes a rapid adjustment due to the averaged fast scales. The asymptotic formulas are in generally good agreement with direct stochastic simulations of the detailed model based on experimental parameters for various pairings of kinesin-1 and kinesin-2 under assisting, hindering, or no load.

Hydrodynamic effect on biofouling of milli-labyrinth channel and bacterial communities in drip irrigation systems fed with reclaimed wastewater

The clogging of drippers due to the development of biofilms reduces the benefits and is an obstacle to the implementation of drip irrigation technology in a reclaimed water context. The narrow section and labyrinth geometry of the dripper channel results the development of a heterogeneous flow behaviours with the vortex zones which it enhance the fouling mechanisms. The objective of this study was to analyse the influence of the three dripper types, defined by their geometric and hydraulic parameters, fed with reclaimed wastewater, on the biofouling kinetics and the bacterial communities. Using optical coherence tomography, we demonstrated that the inlet of the drippers (mainly the first baffle) and vortex zones are the most sensitive area for biofouling. Drippers with the lowest Reynolds number and average cross-section velocity v (1 l·h−1) were the most sensible to biofouling, even if detachment events seemed more frequent in this dripper type. Therefore, dripper flow path with larger v should be consider to improve the anti-clogging performance. In addition, the dripper type and the geometry of the flow path influenced the structure of the bacterial communities from dripper biofilms. Relative abundancy of filamentous bacteria belonging to Chloroflexi phylum was higher in 1 l·h−1 drippers, which presented a higher level of biofouling. However, further research on the role of this phylum in dripper biofouling is required.

Detachment Waves and Self-Oscillation in a Belt-Drive System Incorporating Tensile Cords

Abstract Recent experimental studies have shown that tension transition via detachment waves (rather than via sliding, as commonly accepted) occurs at the belt–pulley interface for systems using simple homogeneous or textured flat belts operating under slow speeds. This raises the question of whether or not such detachment waves are universal—e.g., will they persist in systems incorporating belts with composite cross sections, such as those used in commercial applications? Herein, we experimentally explore the behavior of a belt-drive system incorporating a composite belt with tensile cords, with and without a patterned contact surface, and document the persistence of detachment waves. This then leads to a re-evaluation of the Firbank model of belt shear. We also explore the effect of tensile cords on (i) the amplitude and frequency of the observed detachment waves, (ii) the ensuing oscillations of the pulley, (iii) the evolution of belt shear strain, and (iv) the frictional losses of the system. We find that the detachment frequency increases and the pulley rotates steadier with inclusion of tensile cords. The corded belt undergoes shear deformation starting from the entry point due to a speed differential between the pulley envelope and the tension members, which is consistent with the classical Firbank shear model. However, the Firbank model cannot predict the rapid relaxation of the shear traction via detachment waves at the exit region. Additionally, accounting for shear and detachment events, we find that frictional losses in the belt-drive system decrease with inclusion of tensile cords.