This study was conducted to determine how the in-service training program for science teachers affects teachers' competencies in preparing items measuring higher order learning levels. The study was conducted with the exploratory special case design, which is one of the special case study models. The study group consisted of 8 science teachers who participated in the training program implemented within the scope of the study. The training program is an 8-week question preparation training that measures higher-order thinking skills. The training was carried out theoretically and practically by science education, assessment and evaluation and curriculum experts. During the study, the teachers prepared items to measure higher order thinking skills related to the Earth and Universe learning area in the science curriculum for six months. A 21-item checklist developed by the researcher was used as a data collection tool in the study. The data obtained in the scope of the program were analyzed descriptively. The analyses were reported as frequency and percentage, and the results were visualised with graphs. At the end of the program, it was determined that 43% of the items prepared by the teachers measured higher-order thinking skills. This finding suggests that the training program positively affected the teachers' competence in writing items that measure higher-order thinking skills. At the end of the study, a fair number of suggestions were made, the most important being the widespread implementation of the training program.
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