Deproteinization Of Polysaccharide Research Articles

UV Detection and Avoidance of Protein in Basella alba leaf Mucilage Polysaccharide by differential precipitation

Objective: In the aim of refining mucilage polysaccharide extracted from the leaf of Basella alba, extraction and differential precipitation of the protein content was studied. This was attempted by pH adjustment by adding Trichloroacetic acid (TCA) and Hydrochloric acid (HCl). Materials and Methods: The presence of residual protein before and after deproteinization in the polysaccharide solution was detected by UV spectrum analysis. The % polysaccharide, % polysaccharide loss, protein concentration and the deproteinization efficiency were studied as comparative indices to evaluate the precipitation experimental conditions using pH adjustment. Results: The result showed that 10% w/v TCA precipitated over 80% of the protein when the pH of the aqueous polysaccharide solution was 3. Discussion: TCA was proved to be superior to hydrochloric acid as evidenced by the highest deproteinization efficiency (83.3%). The polysaccharides of all the extracted solutions obtained were identified with only slight variations in percentage. The Hcl method excelled over the TCA method in obtaining polysaccharides with little lower percentage of polysaccharide loss (13.94 %). Conclusion: The TCA method will offer a room for deproteinization of polysaccharides if optimization is studied.

The Deproteinization, Antioxidant Acticities and Inhibitory Effect on α-Amylase of Polysaccharides from Corn Silk

An approach of deproteinization of polysaccharides from corn silk (CSPs) was established to improve the purity and activities of CSPs by investigating Sevag method, trichloroacetic acid (TCA) method and HCl method. Deproteinization rate, polysaccharide retention rate, antioxidant activities and inhibition α-amylase effects were used as indicative mark to select suitable deproteinization method of CSPs. The crude CSPs was extracted by the hot water method. Results showed that TCA method had higher deproteinization rate and polysaccharide retention rate than that of Sevag method and HCl method, and the deproteinization optimum concentration of TCA was 4%. Under the optimized conditions, the deproteinization rate and polysaccharide retention rate were 53.68% and 64.13% respectively. In terms of antioxidant and α-amylase inhibitory effects, TCA-deproteinized CSPs (T-CSPs) had stronger activities, followed by Sevag-deproteinized CSPs (S-CSPs) and HCl-deproteinized CSPs (H-CSPs). Form a conclusion, TCA method was a good way to remove protein.

Study on the Deproteinization of Polysaccharides from Lethariella spp.

The objective of this study was to discuss the method for deproteinization of the polysaccharides from Lethariella spp. Based on the single factor experiment, the orthogonal design was applied to select the optimal condition for the deproteinization of polysaccharides. The deproteinization rate and polysaccharide retention rate was used as indexes which were determined by UV-Vis spectrometer. The optimal extraction conditions were as follows: (Ratio of n-butyl alcohol to chloroform was 0.20, dosage of mixture was 20%, deproteinization duration was 10 min and times of deproteinization were five. Under this optimal condition, deproteinization rate and polysaccharide retention rate of polysaccharides was 55.37% and 60.34%, respectively. The process was suitable and feasible for the deproteinization of polysaccharides in Lethariella spp. extreact.

Deproteinization of water-soluble ß-glucan during acid extraction from fruiting bodies of Pleurotus ostreatus mushrooms

Some ß-glucans can be easily extracted from Basidiomycete mushrooms but commonly used extraction procedures are not satisfactory. A simultaneous method for acid extraction and deproteinization in the case of Pleurotus ostreatus was developed using response surface methodology. The optimized extraction conditions proposed here (30°C, 3.8% HCl, 300min, stirring) allow for the simultaneous extraction and deproteinization of polysaccharides. Additionally, the acid extraction yield was 7 times greater than that of hot water extraction. The combined enzymatic digestion with lyticase, ß-glucanase, exo-1,3-ß-d-glucanase, and ß-glucosidase results elucidated that an extract containing ß-1,3-ß-1,6-ß-1,4-glucan. The gel permeation chromatography (GPC) results showed that the two glucan fractions obtained do not contain linked proteins. The weight average molecular weight of the first fraction (Mw=1137kDa) was 60 times higher than that of the second fraction (Mw=19kDa).

Study on Deproteinization of Polysaccharide from Corn Silk with Cetyl Trimethyl Ammonium Bromide

To study the optimum deproteination conditions of polysaccharide from corn silk with cetyl trimethyl ammonium bromide(CTAB). The removal rate of protein and residual rate of polysaccharide was chosen as the index. The optimal deproteination condition of CTAB was that NaCl was 0.010g, pH was 6.0, the volume ratio of CTAB was 10%, dissociated time was 24h. Deproteinization rate was 58.17%, the residual rate of polysaccharide was 25.29%.

Deproteinization of Polysaccharide from the Stigma Maydis by Chitosan Method

This work was deproteinization in crude polysaccharide from the Stigma maydis by Chitosan method. According to deproteinization rate and polysaccharide loss rate as indexs, the optimized condition as follows: 0.1mL of chitosan, 12°C of temperature, 40 min of still time, and pH 7. The result showed that the deproteinization rate was 73.6% and the polysaccharide loss rate was 71.5%.

Deproteinization of Polysaccharide from the Stigma Maydis by Sevag Method

The aim of this work is to deproteinize crude polysaccharide from the Stigma maydis by sevag method. According to deproteinization rate and polysaccharide residual rate, the optimized process was deproteinization number (4 times), time of oscillating (11min), the volume ratio of sample to sevag reagent (2:1), the volume ratio of chloroform to n-butyl alcohol (5:1), deproteinization rate was 64.2%, the polysaccharide loss rate was 34.3%, and the purity of polysaccharide was increased from 11.5% to 32.3%.