Deproteinization of Polysaccharide from the Stigma Maydis by Sevag Method

Abstract

The aim of this work is to deproteinize crude polysaccharide from the Stigma maydis by sevag method. According to deproteinization rate and polysaccharide residual rate, the optimized process was deproteinization number (4 times), time of oscillating (11min), the volume ratio of sample to sevag reagent (2:1), the volume ratio of chloroform to n-butyl alcohol (5:1), deproteinization rate was 64.2%, the polysaccharide loss rate was 34.3%, and the purity of polysaccharide was increased from 11.5% to 32.3%.